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Citations for The Nest Group :
51 - 100 of 120 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... SP3 beads were magnetically isolated and the digested peptides were desalted for LC-MS/MS analysis using BioPureSPN MINI column (The Nest Group Inc.) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... Digestion was quenched by addition of 3% (v/v) of 100% formic acid (pH ∼2.0 ) and peptides were desalted in a BioPureSPN MACRO spin columns (The Nest Group, Inc.) as described above (Tryptic in-gel digestion) ...
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bioRxiv - Cell Biology 2022Quote: ... The resulting peptides were loaded in pre-equilibrated C18 BioPureSPN mini columns (The Nest Group, Inc.), washed and desalted 3 times with Buffer A (0.1% formic acid in HPLC-grade water) ...
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bioRxiv - Neuroscience 2022Quote: ... and then desalted on C18 columns (The Nest Group), and the desalted peptides were dried by vacuum centrifugation.
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bioRxiv - Microbiology 2022Quote: ... Peptide mixture was purified using C18 column (The Nest Group, Southborough, MA) according to the manufacturer’s protocol and dried under vacuum ...
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bioRxiv - Cancer Biology 2022Quote: ... a pooled digest (3 µg of protein from the fifteen 15 µg samples) was applied to an SCX MicroSpin column (The Nest Group, Inc.) according to manufacturer’s instructions and fractionated using 50 mM ...
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bioRxiv - Cell Biology 2022Quote: ... Fractions were desalted with a MicroSpin C18 column (The Nest Group, Inc) and dried by vacuum centrifugation11.
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bioRxiv - Cancer Biology 2022Quote: ... Digested peptides were then desalted with macro spin column TARGA C18 (The Nest Group) and eluted with 60% acetonitrile (ACN ...
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bioRxiv - Biochemistry 2022Quote: ... Peptides were desalted by solid phase extraction (SPE) using C-18 Micro Spin columns as described by the manufacture’s instruction (The Nest Group). Samples eluted from Micro Spin columns were dried down in SpeedVac concentrator (Thermo Scientific ...
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bioRxiv - Biophysics 2022Quote: ... the precipitated detergent was removed by centrifugation at 10,000g for 15 min and the peptides were separated from the reaction mixture using a C18 spin column (BioPureSPN MINI, The Nest Group, Inc.) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: ... Peptides were purified and desalted using C18 reverse phase columns (The Nest Group, Inc.) following the manufacturer’s recommendations ...
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bioRxiv - Molecular Biology 2022Quote: ... Sample purification was performed over C18 resin (100 µg capacity columns or plates; The Nest Group) using 2% to 80% acetonitrile ...
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bioRxiv - Microbiology 2022Quote: ... Tryptic peptides (50 μg) were loaded onto a BioPureSPN MACRO PolyHYDROXYETHYL A™ column (The Nest Group) in 90% acetonitrile and 150 mM ammonium formate ...
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bioRxiv - Synthetic Biology 2022Quote: ... Peptides were desalted on C18 UltraMicroSpin columns (The Nest Group Inc.) essentially following the instructions provided by the manufacturer but using 300 μl elution solvent consisting of 80% ACN ...
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bioRxiv - Neuroscience 2022Quote: ... pH < 2.0) and desalted at room temperature using a BioPureSPE Mini 96-well plate (20mg PROTO 300 C18; The Nest Group). Briefly ...
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bioRxiv - Systems Biology 2022Quote: ... The peptides were desalted and cleaned-up using C18 columns (The Nest Group, #SEM SS18V) and were resuspended in 5% acetonitrile ...
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bioRxiv - Systems Biology 2022Quote: ... the peptide mix was acidified with formic acid and desalted with a MicroSpin C18 column (The Nest Group, Inc) prior to LC-MS/MS analysis ...
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bioRxiv - Molecular Biology 2022Quote: ... Peptides were desalted using C18 solid phase extraction spin columns (The Nest Group). After desalting ...
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bioRxiv - Immunology 2022Quote: ... and MHC proteins were removed using a Vydac C18 microspin column (The Nest Group, Ipswich, MA). The mixture of MHC and peptides were bound to the column ...
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bioRxiv - Molecular Biology 2022Quote: ... The supernatant was de-salted on ultra-microspin column silica C18 (The Nest Group). De-salted peptides were dried using a SpeedVac vacuum centrifuge concentrator (Thermo Fisher ...
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bioRxiv - Cancer Biology 2022Quote: ... Peptides were desalted using MicroSpin Columns (The Nest Group) and resuspended in 4% formic acid and 3% acetonitrile ...
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bioRxiv - Developmental Biology 2021Quote: ... Digestion was quenched with 0.1% formic acid during the de-salting step with C18 MacroSpin columns (The Nest Group). The effluents from the de-salting step were dried and re-dissolved in 5 µl 70% FA and 35 µl 0.1% TFA ...
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bioRxiv - Evolutionary Biology 2021Quote: ... desalted with a MicroSpin C18 column (The Nest Group, Inc) and dried by vacuum centrifugation ...
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bioRxiv - Evolutionary Biology 2021Quote: ... desalted with a MicroSpin C18 column (The Nest Group, Inc), and dried by vacuum centrifugation.
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bioRxiv - Evolutionary Biology 2021Quote: ... Fractions were desalted with a MicroSpin C18 column (The Nest Group, Inc) and dried by vacuum centrifugation.
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bioRxiv - Neuroscience 2021Quote: ... The resulting peptides purified with C18 MicroSpin columns (The Nest Group, Inc.); for samples with > 60 μg of total protein ...
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bioRxiv - Molecular Biology 2021Quote: ... Peptides were desalted on Ultra Micro Spin Columns C18 (The Nest Group Inc., Ipswich, Massachusetts) (54 ...
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bioRxiv - Molecular Biology 2021Quote: ... The pooled sample was then purified using a silica C18 macrospin column with loading capacity of 30-300 μg of peptide material (The Nest Group, Southborough, MA). Briefly ...
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bioRxiv - Molecular Biology 2021Quote: ... 300A silica (The Nest Group, MA, USA) according to the manufacturer instructions ...
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bioRxiv - Immunology 2021Quote: ... Rounds of solid phase extraction clean-up steps were performed with the use of C18 96-well plates (BioPureSPE Macro 96-Well, 100mg PROTO C18, The Nest Group) as described previously in Messner et al ...
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bioRxiv - Cancer Biology 2021Quote: ... The acidified peptide mixtures were loaded for cleanup into MacroSpin C18 96-well plates (The Nest Group), desalted ...
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bioRxiv - Synthetic Biology 2021Quote: ... Peptides were desalted on C18 UltraMicroSpin columns (The Nest Group Inc.) essentially following the instructions provided by the manufacturer but using 300 μl elution solvent consisting of 80% ACN ...
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bioRxiv - Plant Biology 2021Quote: Peptide samples were purified using Silica C18 Macrospin columns (The Nest Group) according to instructions provided by the manufacturer and peptides were eluted with 200 µL of 70% (v/v ...
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bioRxiv - Cell Biology 2021Quote: ... 50 μl 2 M hydrochloric acid and 50 μl of 5% trifluoroacetic acid were added and peptides collected using BioPureSPN Mini C18 spin columns (Part #HUMS18V from The Nest Group) and analyzed with an Orbitrap Elite Mass Spectrometer (Thermo Scientific ...
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bioRxiv - Systems Biology 2021Quote: ... The samples were then split into six fractions and cleaned by C18 spin columns (The Nest Group, United States).
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bioRxiv - Neuroscience 2021Quote: ... and desalted by MicroSpin C18 Silica columns (The Nest Group. Inc.) according the manufacturers protocol.
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bioRxiv - Microbiology 2021Quote: ... and the peptides were subsequently purified with C18 reverse-phase spin columns according to the manufactures protocol (Microspin Column, SS18V, The Nest Group, Inc). Peptides were then dried in a speedvac and reconstituted in 2% acetonitrile ...
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bioRxiv - Cell Biology 2021Quote: ... peptide mix was acidified with formic acid and desalted with a MicroSpin C18 column (The Nest Group, Inc) prior to LC-MS/MS analysis.
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The human sperm basal body is a complex centrosome important for embryo pre-implantation developmentbioRxiv - Developmental Biology 2021Quote: ... peptide mix was acidified with formic acid and desalted with a MicroSpin C18 column (The Nest Group, Inc, MA, USA) prior to LC-MS/MS analysis ...
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bioRxiv - Systems Biology 2021Quote: ... The column was preceded by a precolumn filter (The Nest Group, Inc ...
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bioRxiv - Plant Biology 2021Quote: ... The samples were acidified to 1% (v/v) with formic acid and solid-phase extraction cleaned using Silica C18 Macrospin columns (The Nest Group). After each of the following steps ...
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bioRxiv - Cancer Biology 2021Quote: Then the digested peptides were cleaned in micro spin columns (The Nest Group Inc.) and dried in CentriVap DNA Vacuum Concentrators (LABCONCO) ...
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bioRxiv - Biochemistry 2021Quote: ... The digested peptides were purified on microspin C18 columns (The Nest Group Inc., HEM S18V) and eluted using 2 × 400 µL of 80% H2O acidified by 0.1% formic acid followed by 2 × 400 µL of 50% acetonitrile (ACN)/H2O acidified by 0.1% formic acid ...
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bioRxiv - Biophysics 2021Quote: ... pH < 2.0) Acidified peptides were desalted using a BioPureSPE Mini 96-Well Plate (20mg PROTO 300 C18; The Nest Group, Inc.) according to standard protocols and dried in a CentriVap Concentrator (Labconco ...
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bioRxiv - Cell Biology 2021Quote: ... The filtrates were then desalted using a reversed-phase C18 column (UltraMicro Spin C18, The Nest group, Southborough, MA) according to the manufacturer’s instructions.
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bioRxiv - Cell Biology 2021Quote: ... desalted using UltraMicroSpin Columns (PROTO 300 C18 300Å; The NEST Group) according to manufacturer’s specifications ...
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bioRxiv - Systems Biology 2020Quote: ... We desalted the clear peptide solution using C18 MicroSpin columns (The Nest Group, 30-300 ug loading capacity). Next ...
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bioRxiv - Cell Biology 2020Quote: ... Supernatants were desalted on stage-tips (The Nest Group). Samples were dried down and solubilized in 5% ACN-0.2% formic acid (FA) ...
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bioRxiv - Molecular Biology 2020Quote: ... Peptides were then separated from beads using a magnetic rack and desalted using C18 silica MicroSpin Columns (The Nest Group). Column elution was performed in 300 μL 80% acetonitrile 0.1% trifluoroacetic acid and peptides were dried by centrifugal vacuum.
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bioRxiv - Genetics 2020Quote: ... 60 µl of Fe-NTA resin slurry was transferred to individual Silica C18 MicroSpin columns (The Nest Group) pre-equilibrated with 100 µl of 80% ACN ...