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Citations for Amresco :
1 - 50 of 82 citations for Dengue Virus Serotype 2 Envelope Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: Total protein extraction from cells was performed using 1.5x Laemmli buffer [15% glycerol (Amresco #M152), 3% SDS (Sigma #L5750) ...
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bioRxiv - Immunology 2020Quote: ... Native cell wall proteins were precipitated from the supernatant using 80% w/v (NH4)2SO4 (Amresco, OH) at 4 °C overnight ...
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bioRxiv - Cell Biology 2022Quote: ... 2%DMSO (Amresco), 200μM Butylated hydroxyanisole (Sigma-Aldrich) ...
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bioRxiv - Immunology 2021Quote: ... 50uM 2-mercaptoethanol (Amresco), 1% MEM vitamin solution (Gibco) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Cells were inoculated into 5 mL of YP (10 g/L yeast extract, 20 g/L peptone) + 2% galactose (Amresco) and grown to mid-log phase (48 – 55 hours depending on the strain ...
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bioRxiv - Cell Biology 2019Quote: ... 0.1 mM 2-Mercaptoethanol (AMRESCO), 1000 U/mL mouse recombinant Leukemia inhibitory factor (LIF ...
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bioRxiv - Immunology 2020Quote: ... and 50 μM 2-mercaptoethanol (Amresco) at 37°C with 5% CO2 ...
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bioRxiv - Immunology 2020Quote: ... and 50 μM 2-mercaptoethanol (Amresco).
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bioRxiv - Immunology 2020Quote: ... and 50 μM 2-mercaptoethanol (Amresco) for 3 days with 0.5 μg/mL LPS (Invivogen ...
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bioRxiv - Immunology 2020Quote: ... and 50 μM 2-mercaptoethanol (Amresco) and 5 μg/mL puromycin (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2021Quote: ... and 2% SDS (Amresco cat#; M112-500ML) was added ...
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bioRxiv - Molecular Biology 2019Quote: ... 2 mM L-Dithiothreitol (DTT, M109, Amresco), 5% glycerol ...
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Mck1 defines a key S-phase checkpoint effector in response to various degrees of replication threatsbioRxiv - Molecular Biology 2019Quote: ... then with 2 mg/ml Proteinase K (Amresco) in 100 mM EDTA ...
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bioRxiv - Cell Biology 2019Quote: ... total protein lysates was isolated using NP-40 buffer (Amresco, J619) supplemented with protease/phosphatase inhibitor cocktail (Cell Signaling Technology ...
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bioRxiv - Biochemistry 2019Quote: ... and 0.2 mM tris(2-carboxyethyl)phosphine (Amresco, K831-26) for structural studies.
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bioRxiv - Biophysics 2024Quote: ... Protein estimation was done using the Bradford’s method using Bradford reagent (Amresco) [1-3] ...
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bioRxiv - Bioengineering 2021Quote: ... Gels were stained with 2 µg/mL ethidium bromide (Amresco, Solon, OH) in 1 X TBE and rocked for 15 min ...
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bioRxiv - Microbiology 2022Quote: ... samples were stored in PBS containing 2 % formaldehyde (VWR Life Science AMRESCO). For the intracellular staining of infected U87.ACE2+ cells ...
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bioRxiv - Microbiology 2022Quote: ... samples were stored in PBS containing 2 % formaldehyde (VWR Life Science AMRESCO).
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bioRxiv - Microbiology 2022Quote: ... samples were stored in PBS containing 2 % formaldehyde (VWR Life Science AMRESCO). Acquisition of all samples was done on a BD FACSCelesta flow cytometer (BD Biosciences ...
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bioRxiv - Cancer Biology 2019Quote: ... which was blocked in 2% bovine serum albumin (Amresco, Solon, Ohio, USA) and then incubated overnight at 4° ...
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bioRxiv - Microbiology 2022Quote: ... samples were stored in PBS containing 2 % formaldehyde (VWR Life Science AMRESCO). Acquisition of all samples was done on a BD FACSCelesta flow cytometer (BD Biosciences ...
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bioRxiv - Biochemistry 2020Quote: ... Equal amounts of protein were run on a SDS-PAGE (10% NEXT Gel, Amresco) and transfered to a PVDF membrane ...
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bioRxiv - Neuroscience 2021Quote: ... the tissue was submerged in a diaminobenzidine (DAB) solution (2 DAB tablets, Amresco E733 containing 5 mg DAB per tablet ...
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bioRxiv - Molecular Biology 2021Quote: ... YPRaf medium uses YP medium components supplemented with raffinose (2% w/v, Amresco) and antimycin A (1 mg/ml ...
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bioRxiv - Cancer Biology 2020Quote: ... 2% Sodium deoxycholate and 0.2% SDS) with 1% protease inhibitor (Amresco M250-1ML). Cell lysates were sonicated using Diagenode Bioruptor Plus for 15 total minutes (30 seconds on and 30 seconds off) ...
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bioRxiv - Biochemistry 2020Quote: ... and protein expression was induced with final concentration of 0.2% (w/v) L-arabinose (AMRESCO) at 37°C when OD600 reached 0.4~0.6 ...
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bioRxiv - Physiology 2021Quote: ... lysed in protein loading buffer (50 mM Tris, pH 6.8, 100 mM dithiothreitol (Amresco, 0281), 2% SDS (Sigma-Aldrich ...
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bioRxiv - Developmental Biology 2019Quote: ... The supernatant was transferred to another tube and 2% SDS (Amresco cat# M112-500ML) was added ...
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bioRxiv - Genetics 2021Quote: ... DNA fragments between 400-700 bp were selected on a 2% agarose gel (Amresco) and purified using a QIA quick Gel Extraction Kit (Fermentas ...
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bioRxiv - Biochemistry 2019Quote: ... For unlabeled protein expression was induced with 1 mM isopropylthio-β-D-galactoside (IPTG; Amresco, 97061) and cultures were grown for 18 hours at 18°C ...
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bioRxiv - Cell Biology 2021Quote: ... Recombinant proteins were checked and quantified in a polyacrylamide gel stained with Comassie Brilliant Blue (Amresco, 0472). The proteins were stored at −80°C ...
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bioRxiv - Molecular Biology 2021Quote: ... one-fifth of each sample was then electrophoresed on a 2% high-resolution blend agarose (Amresco) gel in 1x Tris-acetate-EDTA buffer (40 mM Tris-acetate ...
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bioRxiv - Developmental Biology 2019Quote: ... the fish were fixed with 2% PFA and 0.05% Glutaraldehyde in 80% HistoChoice (H120, Amresco Inc.) with 1% sucrose and 1% CaCl2 at 4□ for 24h ...
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bioRxiv - Cell Biology 2019Quote: ... 24-well plates with each well containing 2 mL NGM medium with 1 mM IPTG (Amresco) and 75 mg/L ampicillin were prepared in advance and stored at 4°C until needed ...
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bioRxiv - Biochemistry 2020Quote: ... PBS containing 2 μg/mL Hoechst 33342 and 4 μg/mL pyronin Y (Amresco, cat# 0207) was added to the cells to stain DNA and RNA ...
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bioRxiv - Immunology 2020Quote: ... ELISA plates were thoroughly washed 2 times each using 1× PBS+0.05% Tween 20 (AMRESCO, USA) and 1× PBS ...
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bioRxiv - Molecular Biology 2024Quote: ... Membranes were then blocked for 1-2 hours in 5% powdered milk (Amresco, M203-10G-10PK) in PBST ...
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bioRxiv - Microbiology 2020Quote: ... approximately equal loading and transfer was confirmed by total protein staining of the nitrocellulose membrane with Ponceau S (Amresco). Chemiluminescent detection followed incubation with polyclonal antiserum or monoclonal antibody ...
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bioRxiv - Immunology 2020Quote: Supernatant proteins in the native form were precipitated from the supernatant using 80% w/v (NH4)2SO4 (Amresco, OH) at 4 °C overnight ...
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bioRxiv - Molecular Biology 2023Quote: ... Transformants scraped from densely plated transformation plates were inoculated into fresh SC-His medium with 2% raffinose (Amresco, J392) at 0.25 x 107 cells/ml and grown until 0.5-0.8 x 107 cells/ml ...
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bioRxiv - Biochemistry 2021Quote: ... the beads were washed with lysis buffer and proteins were eluted in 2x Laemmli loading buffer containing 1 mM dithiothreitol (DTT; Amresco, Solon, OH) for 30 min ...
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The formation of a fuzzy complex in the negative arm regulates the robustness of the circadian clockbioRxiv - Molecular Biology 2022Quote: ... and the percentage of FRH in the final product was visually estimated based on the percentage of the FRH band relative to other bands (∼30% FRH) when the eluted protein was visualized on a Coomassie-stained gel (Amresco, 0472-25G). Expression and purification of FRHΔ100 was verified by SDS-PAGE analysis (ThermoScientific ...
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bioRxiv - Cancer Biology 2019Quote: ... Proteins were transferred from the gel to 0.45 μm pore size nitrocellulose membrane (Maine Manufacturing, Sanford, ME; 1215471) and total protein visualized using Ponceau S (Amresco, Radnor, PA; K793). The membrane was blocked with 2.5% (wt/vol ...
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bioRxiv - Immunology 2021Quote: ... Antibodies were diluted in PBS with 2% FBS and samples were stored in PBS containing 1% formaldehyde (VWR Life Science AMRESCO). For intracellular staining ...
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bioRxiv - Cell Biology 2023Quote: ... and B103 cells were incubated in 6 mL of medium containing 0.5% 3-(4,5-dimethylthiazol-2-yl)-2,5-di-phenyltetrazolium bromide (MTT; Amresco Inc., OH, USA) at 37 °C for 90 min and ASCs for 3 h ...
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bioRxiv - Microbiology 2024Quote: ... Cytotoxicity was assessed using the MTT assay (3-[4,5-methylthiazol-2-yl]-2,5-diphenyl-tetrazolium bromide, Amresco, Solon, OH, USA), following the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2020Quote: ... Proteins were transferred from the gel to 0.45 m pore size μ nitrocellulose membrane (Maine Manufacturing, Sanford, ME; 1215471) and total protein visualized using Ponceau S (Amresco, Radnor, PA; K793). The membrane was blocked with 2.5% (wt/vol ...
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bioRxiv - Microbiology 2022Quote: ... Cells were grown in standard LB medium (Amresco) with antibiotics and inducers (if necessary) ...
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bioRxiv - Genetics 2022Quote: ... Cells were counterstained with nuclear fast red (Amresco, 1B1369) and dehydrated before mounting.