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Citations for PromoCell :
551 - 600 of 628 citations for Primary Human Aortic Smooth Muscle Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... The cell pellet was washed and resuspended in Keratinocyte Growth Medium-2 (KGM2, PromoCell, Heidelberg, Germany) containing bovine pituitary extract (4 μl/mL) ...
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bioRxiv - Bioengineering 2023Quote: ... and cryopreserved at 10 million cells/mL in Cryo SFM freezing medium (C-29910; PromoCell GmbH).
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bioRxiv - Cancer Biology 2023Quote: ... or serum-free Cancer Cell Line Media XF (CCLM XF, C-28077, PromoCell GmbH, Heidelberg, Germany), depending on the individual cell line as follows ...
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bioRxiv - Cell Biology 2024Quote: ... USA (ACBRI 376) and cultured in Endothelial Cell Growth Medium 2 (EGM2) media (C-22111, Promocell) supplemented with 2% foetal calf serum (FCS) ...
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bioRxiv - Biochemistry 2024Quote: ... Cells were split at a 1:2 ratio every 3 days by trypsinization (Promocell cat# C41210), followed by centrifugation at 220 x g for 3 min before resuspending and reseeding in SMC Basal medium 2 supplemented with Growth Medium 2 supplement Mix ...
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bioRxiv - Cancer Biology 2022Quote: All cell lines were validated and tested negative for mycoplasma (PCR Mycoplasma Test Kit, Promocell, Heidelberg, Germany). Cells were cultured in a humidified incubator at 37 °C and 5% CO2according to the recommendations and protocols of ATCC ...
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bioRxiv - Cell Biology 2023Quote: ... were purchased from Lonza (Walkersville, MD) and cultured in endothelial cell growth medium MV2 (ECG MV2, C-22121) (PromoCell, Heidelberg, Germany) and 100U/mL penicillin-streptomycin (Gibco ...
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bioRxiv - Molecular Biology 2023Quote: ... this serum-free conditioned medium was collected and Mesenchymal Stem Cell Growth Medium 2 (#C-28009, PromoCell) added to cells for further cultivation ...
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bioRxiv - Molecular Biology 2023Quote: Small extracellular vesicles (sEVs) were isolated from umbilical cord matrix derived mesenchymal stem cells (#C-12971, PromoCell)) via differential ultracentrifugation ...
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bioRxiv - Microbiology 2020Quote: ... and 672447) were expanded in 75 cm2 tissue culture flasks using airway epithelial cell growth medium (Promocell, Germany) until 60-70% confluent ...
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bioRxiv - Cell Biology 2022Quote: ... HBECs were expanded for 1 to 4 passages in airway epithelial cell growth media (AEGM, Promocell, Heidelberg, DE) and transitioned to Pneumacult Ex+ (Stem Cell Technologies ...
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bioRxiv - Cell Biology 2020Quote: ... C-12281 and C-12212 respectively) and cultured in Endothelial Cell Gowth medium MV2 with supplementMix (PromoCell, C22022). For rosettes formation ...
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bioRxiv - Cancer Biology 2022Quote: HPK cells were freshly isolated from neonatal foreskin and cultured in Keratinocyte Growth Medium 2 (PromoCell, Heidelberg, Germany).
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bioRxiv - Bioengineering 2023Quote: ... Primary TCs were obtained from Amsbio (#MBE-F-TM and #MLE-F-TM) and cultured in Mammary Epithelial Cell Growth Medium (#C-21010, Promocell). Human umbilical vein ECs (HUVECs ...
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bioRxiv - Microbiology 2023Quote: ... Becton Dickinson) using PneumaCult™-Ex Plus Medium (Stemcell) or the Airway Epithelial Cell Grow Medium Kit (Promocell). Hereafter ...
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bioRxiv - Cell Biology 2023Quote: ... were maintained in Endothelial Cell Basal Medium (EBM-2) supplemented with Endothelial Growth Medium-2 Supplement Pack (PromoCell). Pooled HAECs (PromoCell ...
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bioRxiv - Immunology 2024Quote: ... was applied to HUVECs before seeding 15,000 cells resuspended in low-serum media (0.5% FBS and VEGF-negative, Promocell #C-22010 diluted 1:3 in basal media # C-22010B ...
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bioRxiv - Physiology 2023Quote: ... were seeded at 4000 cells/cm2 density and cultured in the respective Fibroblast Growth Medium (C-23025, PromoCell).
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bioRxiv - Cell Biology 2020Quote: ... Isolated TECs were seeded onto collagen-coated Petri dishes and cultured in renal epithelial cell medium (C-26130, PromoCell) supplemented with growth factors for TEC growth.
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bioRxiv - Bioengineering 2021Quote: ... The epithelial cells were cultured and expanded in Airway Growth Medium (Airway Epithelial Growth Medium Kit, Promocell, C-21160) supplemented with 1% penicillin-streptomycin ...
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bioRxiv - Developmental Biology 2021Quote: ... These cells were cultured in 15% FBS (GemCell, 100-500) and 5% Growth Medium Supplement Mix (PromoCell, C-39365) in Skeletal Muscle Cell Basal Medium (PromoCell ...
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bioRxiv - Bioengineering 2022Quote: ... HUVECs were obtained from Lonza™ and cultured in Endothelial Cell Growth Medium-2 (EGM-2, PromoCell® C-22111 or Lonza™ CC-3162). Human primary pericytes were purchased from PromoCell® (PromoCell® ...
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bioRxiv - Microbiology 2020Quote: ... The culture medium was changed to remove detached cells and treatments as indicated in endothelial basal medium (EBM, PromoCell) in the presence of 2 mM hydroxyurea to prevent cell proliferation ...
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bioRxiv - Immunology 2020Quote: ... Treatment protocols (except for those using MucilAir™ cells) were conducted using ‘treatment media’ (EGM-2 medium (Promocell, Germany) supplemented with 2% FBS (Promocell ...
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bioRxiv - Cancer Biology 2021Quote: Cell cultures tested for mycoplasma using a commercially available kit (PCR Mycoplasma Test Kit I/C, PromoCell, Heidelberg, Germany) shows absence of a positive band at ∼270bp ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were resuspended and cultured in 0.5% gelatin-coated culture well (12-well format) in EGM2 medium (PromoCell, C30140) supplemented with 10% FBS ...
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bioRxiv - Cell Biology 2021Quote: ... were seeded at a density 1.5 × 105 cells/well in 12-well plates and cultured in a myocyte growth medium (C-22170, PromoCell) containing 0.5 ng/ml epidermal growth factor ...
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bioRxiv - Cell Biology 2022Quote: ... were purchased from PromoCell and cultured in 0.5% gelatin-coated dishes in in endothelial cell growth medium MV2 supplemented with C-39225 supplement mix (PromoCell) (Tornavaca et al. ...
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bioRxiv - Cell Biology 2022Quote: ... University Medical Center of the Johannes Gutenberg University Mainz) and were cultured on 0.2% gelatin coating in Endothelial Cell Growth Medium (Cat# C-22010, PromoCell). HT-29 cells were purchased from ATCC (ATCC HTB-38 ...
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bioRxiv - Microbiology 2023Quote: ... were obtained from Promocell and maintained as per the manufacturer’s instructions in 75 cm2 flasks at 37°C with 5% CO2 in endothelial cell growth medium (Promocell) with 100 U/mL penicillin ...
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bioRxiv - Cell Biology 2022Quote: ... Lung endothelial cells were seeded on 0.1% gelatine-coated wells and cultured in a 1:1 mixture of EGM2 (PromoCell) and fully supplemented Dulbecco’s modified Eagle medium (DMEM ...
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bioRxiv - Molecular Biology 2022Quote: ... and left for 3 days until 100 % confluency in full supplemented ECM MV2 cell media (Promocell, cat. #C-22022). 15 h before transfection was placed in serum-reduced ECM MV2 media (containing 10 % of originally cell growth supplement MV2) ...
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bioRxiv - Cell Biology 2023Quote: ... Isolated TECs were seeded onto collagen-coated Petri dishes and cultured in renal epithelial cell medium (C-26130, PromoCell) supplemented with growth factors for TEC growth27.
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bioRxiv - Physiology 2023Quote: ... were seeded at 6000 cells/cm2 density and culture in the respective Pericyte Growth Medium 2 (C-28041, PromoCell). Upon reaching 90% confluency ...
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bioRxiv - Cell Biology 2020Quote: ... into the vein for 20 minutes at 37°C and subsequently cultured in endothelial cell growth medium purchased from PromoCell supplemented with 1% penicillin/streptomycin and used up to passage 4 for all indicated experiments.
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bioRxiv - Cell Biology 2021Quote: ... containing 5% heat-inactivated fetal calf serum (FCS) with supplements (endothelial cell growth supplement: epidermal growth factor, basic fibroblast growth factor, heparin, and hydrocortisone, from PromoCell). Primary human adipose microvascular endothelial cells (HAMEC ...
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bioRxiv - Cancer Biology 2020Quote: ... Mycoplasma status was defined negative for all cell lines by PCR (PCR Mycoplasma Test Kit I/C, PromoCell GmbH, Germany) and cell lines were authenticated by Short Tandem Repeat genotyping (Eurofins Genomics ...
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bioRxiv - Developmental Biology 2022Quote: ... Isolated HBE cells were maintained and expanded (one passage) in T75 flasks in hormone- and growth factor-supplemented airway epithelial cell growth medium (AEGM, ready-to-use; PromoCell) at 37 °C in a 5% CO2 incubator ...
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bioRxiv - Cancer Biology 2020Quote: ... Kyse30 and FaDu cells were stably transfected with SLUG-Myc in the 141-pCAG-3SIP vector with MATra transfection reagent (PromoCell) using 1 μg/ mL puromycin (Sigma ...
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bioRxiv - Immunology 2022Quote: ... Airway epithelial cells were taken using 2mm diameter cytology brushes from 3rd to 5th order bronchi and cultured in Airway Epithelial Cell medium (PromoCell) in submerged culture.
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bioRxiv - Cancer Biology 2019Quote: ... UWB1-SyR12 and UWB1-SyR13 cell lines were cultured in RPMI 1640/Mammary Epithelial Growth Media (1:1; Hyclone/PromoCell) supplemented with 3% FBS ...
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bioRxiv - Cell Biology 2022Quote: ... Once expanded, all endothelial cells (HUVEC, hCMEC/D3, HDLEC, HDBEC, and BOECs) were maintained in either ECGM or ECGM MV2 (Promocell) and endothelial phenotype regularly assessed by flow cytometry using anti-CD31-AF488 and anti-VE-cadherin-Biotin antibodies ...
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bioRxiv - Microbiology 2022Quote: ... were cultured in Airway Epithelial Cell Growth Medium (basal medium supplemented with BPE, EGF, insulin, hydrocortisone, epinephrine, triiodo-L-thyronine, transferrin and retinoic acid; PromoCell) at 37 °C in a 5% CO2 atmosphere ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were cultured on Corning CellBind or collagen-coated dishes in an equal mixture of ECGM-2 (Promocell, C-22111) and M-199 (Pan Biotech ...
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bioRxiv - Microbiology 2023Quote: ... and cells were resuspended in 12 mL warm supplemented airway culture media (Airway Epithelial Cell Growth Basal Media, Promocell; supplemented with Growth Medium SupplementPack [#C-39160, Promocell] ...
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bioRxiv - Microbiology 2023Quote: ... Primary cultures were established by thawing the cryovial in a 37°C water bath for 2 minutes and resuspending cells into warmed airway culture media (Airway Epithelial Cell Growth Basal Media, #C-21260, Promocell; supplemented with Growth Medium SupplementPack, #C-39160, Promocell), and plating at a density of 1.5 × 103 cells/cm2 into a T25 flask ...
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bioRxiv - Cell Biology 2023Quote: ... HDMECs and HCMECs were routinely cultured in Endothelial Cell Growth Medium MV 2 (EGM MV2; C-22022) with supplement mix (C-39226, all PromoCell), NHDFs and HCFs in Fibroblast Growth Medium (FGM ...
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bioRxiv - Pathology 2023Quote: HMEC-1 cells (ATCC: CRL-3243) were cultured in EGM-2 MV (EBM-2 added with growth factors and other supplements, PromoCell). Lipofectamine RNAiMAX (Thermo Fisher Scientific ...
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Shear stress and very low levels of ligand synergize to activate ALK1 signaling in endothelial cellsbioRxiv - Cell Biology 2023Quote: ... were cultured in a sterile humidified incubator in complete endothelial cell growth medium (EC Growth Medium 2/EGM2, containing 2% FBS; Cat: C-22011, Promocell) and 1 × antibiotic-antimycotic (Cat ...
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bioRxiv - Immunology 2023Quote: Epidermal single-cell suspensions were isolated as previously described for fluorescence activated cell sorting and cultured on fibronectin-coated dishes in keratinocytes growth medium 2 (Promo2) supplemented with 0.02 mM CaCl2 (Promo Cell), growth supplements (1:40 ...