Labshake search
Citations for PromoCell :
151 - 200 of 201 citations for 8 Chloro 2 methylimidazol 1 2 a pyrazine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2022Quote: ... HLFs were seeded in four wells of a 24-well plate in antibiotic free medium containing 2 % (Lonza, PromoCell) or 10 % (DZL ...
-
bioRxiv - Cell Biology 2023Quote: ... with added (TGF) β-inhibitory molecule SB431542 (10 μM) and (2) Smooth Muscle Growth Medium (SM2) (PromoCell, Cat # C22062) with added SB431542 (10uM) ...
-
bioRxiv - Immunology 2023Quote: Human keratinocytes (K2; gift from Dr. Miosge, University Göttingen) were cultured in Keratinocyte Growth Medium 2 (Promocell, Heidelberg, Germany) at 37°C 5% CO2 and 95% humidity following the manufacturer protocol ...
-
bioRxiv - Physiology 2023Quote: ... were seeded at 6000 cells/cm2 density and culture in the respective Pericyte Growth Medium 2 (C-28041, PromoCell). Upon reaching 90% confluency ...
-
bioRxiv - Cell Biology 2021Quote: ... cell viability was evaluated by trypan blue dye exclusion and an appropriate number of cells was resuspended in Renal Epithelial Cell Growth Medium 2 (Promocell). This suspension was seeded into collagen I-coated cell culture dishes (Corning ...
-
bioRxiv - Cell Biology 2022Quote: ... Cells were cultured on Corning CellBind or collagen-coated dishes in an equal mixture of ECGM-2 (Promocell, C-22111) and M-199 (Pan Biotech ...
-
bioRxiv - Microbiology 2022Quote: ... and cultured in 1% gelatine coated cell cultures flasks with Endothelial Cell Growth Medium MV-2 kit according to the manufacturer’s protocol (#C-22121, PromoCell-PromoKine), LMECs were only used up to passage 10 to ensure organ specificity ...
-
bioRxiv - Physiology 2023Quote: ... Complete SMC medium was replaced with serum free SMC basal media (SMC Basal Medium 2; C-22262, PromoCell. Heidelberg, Germany) and allowed to rest for 6 hours at 37°C in 5% CO2 ...
-
bioRxiv - Microbiology 2023Quote: ... Primary cultures were established by thawing the cryovial in a 37°C water bath for 2 minutes and resuspending cells into warmed airway culture media (Airway Epithelial Cell Growth Basal Media, #C-21260, Promocell; supplemented with Growth Medium SupplementPack ...
-
bioRxiv - Cell Biology 2023Quote: ... HDMECs and HCMECs were routinely cultured in Endothelial Cell Growth Medium MV 2 (EGM MV2; C-22022) with supplement mix (C-39226, all PromoCell), NHDFs and HCFs in Fibroblast Growth Medium (FGM ...
-
bioRxiv - Cell Biology 2023Quote: ... were cultured in HDF growth media supplemented with FBS (2% v/v) basic fibroblast growth factor (recombinant human; 1ng/ml) and insulin (5 μg/ml) (all from PromoCell). All cells were grown in a humidified environment at 37°C with 5% CO2 ...
-
bioRxiv - Immunology 2023Quote: Epidermal single-cell suspensions were isolated as previously described for fluorescence activated cell sorting and cultured on fibronectin-coated dishes in keratinocytes growth medium 2 (Promo2) supplemented with 0.02 mM CaCl2 (Promo Cell), growth supplements (1:40 ...
-
bioRxiv - Pathology 2024Quote: ... HUVECs (passage 2-4; isolated from donors were provided by Dr Siow) cultured in endothelial cell growth medium (EGM; PromoCell) were resuspended at 3 x 104 cells/ml and plated on top of the NHDF (500 μl/well for 24 well plates) ...
-
bioRxiv - Immunology 2024Quote: NHEK-svTERT3-5 (Evercyte, Cat: CLHT-011-0026-5) keratinocytes were cultured in KGM-2 media (Promocell, Cat: C-20111). Keratinocytes were plated at 2.5×105/mL in a 48-well plate and left to attach overnight ...
-
bioRxiv - Cancer Biology 2022Quote: ... for 30 min at 37 °C before seeding tumor-derived endothelial cells (30,000 cells/insert; 100 μl endothelial cell growth medium MV-2, PromoCell Heidelberg, Germany). Then ...
-
bioRxiv - Cell Biology 2023Quote: ... were cultured in Endothelial Cell Growth (ECG) Medium supplemented with 2% v/v fetal calf serum (Cat. #: C-22111, PromoCell, Germany). Subculturing and maintenance cultures were performed using 3 × 105 cells at 37°C in a 5% CO2 atmosphere ...
-
bioRxiv - Molecular Biology 2023Quote: ... Human Umbilical Vein Endothelial Cells were purchased and seeded onto 24-well Nunc plates at 25 000 cells per well in Endothelial Cell Growth Medium 2 (PromoCell, Germany). Macrophage supernatant experiments were conducted by preparing 2X Growth Medium and mixing 50:50 with THP-1 supernatant ...
-
bioRxiv - Developmental Biology 2021Quote: ... Epinephrine (0.5 μg/ml), Triiodo-L-thyronine (4pg/ml), and Transferrin (recombinant human, 5 μg/ml) (Growth Medium 2 SupplementPack, PromoCell, C-39605).
-
bioRxiv - Cell Biology 2021Quote: ... Fibroblasts were purified by plating on plastic tissue culture dishes for 2 hours in Fibroblast Growth Medium 3 (PromoCell Cat #c-23130), then media was refed to eliminate cells that had not stuck ...
-
bioRxiv - Pathology 2022Quote: ... minced finely and passed through a 70 µm cell strainer in RPTEC media (Renal Epithelial Cell Growth Medium 2, PromoCell, # C-26130) supplemented with Penicillin-Streptomycin Solution (100U/mL ...
-
bioRxiv - Cell Biology 2021Quote: ... Primary mouse proximal tubule epithelial cells were isolated from mouse renal cortices as previously described (Legouis et al, 2015) and were cultured in Basal Medium 2 phenol red-free (PromoCell, #C-22216) supplemented in the same way as HK-2 cells ...
-
bioRxiv - Bioengineering 2020Quote: GFP-labeled human umbilical vein endothelial cells (GFP-hUVECs) (Angioproteomie, MA, USA) were cultured in Endothelial Growth Medium 2 (PromoCell, Heidelberg, Germany). Adipose derived human mesenchymal stem cells (hAMSCs ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... Isolated TECs were cultured under sterile conditions at 37°C and 5% CO2 in conditioned Renal Epithelial Cell Growth Medium 2 (PromoCell, Heidelberg, Germany). The TECs isolated by this method have been reported to be mainly proximal tubule epithelial cells (65).
-
bioRxiv - Immunology 2020Quote: ... HPMEC-M or HPMEC-F (primary human pulmonary microvascular endothelial cells-male or female, used at passage 2–6, Promocell, Heidelberg, Germany). Human lung alveolar macrophages (AM ...
-
bioRxiv - Physiology 2023Quote: Primary VSMC lines from C57Bl/6 and SGK-1flox+/+ aortic biopsies were established using an accepted outgrowth technique as previously described.15 Isolated VSMCs were maintained in SMC specific growth media with added supplement pack (SMC Growth Medium 2; C-22062, PromoCell, Heidelberg, Germany) at 37°C in 5% CO2 ...
-
bioRxiv - Cell Biology 2023Quote: ... were seeded within appropriate cell densities into tissue culture plates in assay medium – Fibroblast Basal medium-2 (phenol red-free; PromoCell, C-23225) containing 2% FBS ...
-
bioRxiv - Developmental Biology 2023Quote: ... were grown in ECGM-2 medium supplemented with VEGF for 24 hours before the addition of 100,000 human pericytes (Promocell, Cat# C-12980), with or without BMP9 ...
-
bioRxiv - Biochemistry 2023Quote: ... were disseminated and cultivated for 96 hours in 4-well chamber slides in 90% EMEM medium supplemented with 10% FBS (Wi-38 cells) and 98% Fibroblast Growth Medium/2% supplement mix (Promocell, NHDF cells), respectively ...
-
bioRxiv - Cell Biology 2023Quote: ... epinephrine (0.5 µg/ml), Triiodo-L-thyronine (4 pg/ml), and transferrin (recombinant human, 5 µg/ml) (Growth Medium 2 Supplement Pack, PromoCell, C-39605). Cells were allowed to grow until 80-90% confluent and culture medium promotes epithelial cell survival ...
-
bioRxiv - Microbiology 2024Quote: ... HUVEC cells were cultured in cell culture flasks coated with gelatin in endothelial cell basal medium supplemented with 2% FCS and growth factors (Endothelial Cell Growth Medium kit) (PromoCell, Heidelberg, Germany). For infection experiments ...
-
bioRxiv - Cell Biology 2020Quote: ... Culture medium for h-iECs was prepared by adding Endothelial Cell Growth medium 2 kit supplements into basal medium (except for hydrocortisone, PromoCell, Cat No. C22111) with 1x GlutaMax supplement and 10 μM SB431542.
-
bioRxiv - Cell Biology 2020Quote: ... 1% P/S in fibronectin (1 µg/mL, Promocell) coated T75 flasks (Sarstedt ...
-
bioRxiv - Bioengineering 2021Quote: ... and the adherent cells (somatic cells) were expanded on PLL-coated plates in a 1:1:1 mixture of Endothelial Cell Growth Medium (Promocell, C-22010), Sertoli Cell Medium (Sciencell ...
-
bioRxiv - Bioengineering 2021Quote: ... Cells were sorted into somatic and germ fractions by overnight plating: the adherent cells were expanded in a 1:1:1 mixture of Endothelial Cell Growth Medium (Promocell, C-22010), Sertoli Cell Medium (Sciencell ...
-
bioRxiv - Bioengineering 2019Quote: DiR (1, 1-dioctadecyl-3, 3, 3, 3-tetramethylindotricarbocyanine iodide) (PromoCell GmbH, Heidelberg, Germany) loaded BSA (Seracare ...
-
bioRxiv - Cancer Biology 2022Quote: ... 1× Lipofuscin Autofluorescence Quencher (Promocell) was applied for 30 seconds prior to fluorescence labelling ...
-
bioRxiv - Immunology 2021Quote: ... mixed 1:1 (v/v) with 2x Monolayer medium (Airway Epithelium Cell Basal Medium, PromoCell, supplemented with 2 packs of Airway Epithelial Cell Growth
-
bioRxiv - Microbiology 2021Quote: ... cells were washed once with 1:1 plain DMEM and Airway Epithelial Cell Growth medium (Promocell) supplemented with BSA and supplement kit (ALI culture medium ...
-
bioRxiv - Cell Biology 2022Quote: ... Lung endothelial cells were seeded on 0.1% gelatine-coated wells and cultured in a 1:1 mixture of EGM2 (PromoCell) and fully supplemented Dulbecco’s modified Eagle medium (DMEM ...
-
bioRxiv - Cancer Biology 2019Quote: ... UWB1-SyR12 and UWB1-SyR13 cell lines were cultured in RPMI 1640/Mammary Epithelial Growth Media (1:1; Hyclone/PromoCell) supplemented with 3% FBS ...
-
bioRxiv - Biophysics 2024Quote: ... mAir: a 1:1 mix of Dulbecco’s modified eagle medium and Airway epithelial cell growth medium (AECGM, PromoCell, C-21160) with AECGM supplements and 50CnM EC23 (previously described in 46. ...
-
bioRxiv - Cancer Biology 2022Quote: ... was diluted 1:10 in Endothelial Cell Basal Medium MV (PromoCell, Heidelberg, Germany). Ten microliters of the diluted dye was added to each upper chamber and incubated for 20 min at RT ...
-
bioRxiv - Cell Biology 2023Quote: ... 1 µg/ml ascorbic acid and 0.2 µg/ml hydrocortisone (Promocell, Heidelberg, Germany). HEK293T cells and HUVECs were cultivated at 37°C in a humidified incubator supplied with 5% CO2
-
bioRxiv - Bioengineering 2023Quote: ... 1 ng/mL bFGF (all from the Growth Medium SupplementPack C-39210; PromoCell GmbH) – this will be referred to as “co-culture medium” in the following.
-
bioRxiv - Cell Biology 2022Quote: ... each gel was supplied with 1 ml of Mammary Epithelial Cell Growth Medium (Promocell, c-21010) supplemented with Mammary Epithelial Cell Growth supplement (Sciencell ...
-
bioRxiv - Cell Biology 2022Quote: ... HBECs were expanded for 1 to 4 passages in airway epithelial cell growth media (AEGM, Promocell, Heidelberg, DE) and transitioned to Pneumacult Ex+ (Stem Cell Technologies ...
-
bioRxiv - Bioengineering 2022Quote: ... a co-culture of 7.5×103 Red Fluorescent Protein-tagged HUVEC (RFP-HUVEC, 2Bscientific, UK) and 1×105 Normal Human Dermal Fibroblasts (NHDF, Promocell) or Human Mesenchymal Stem cells (MSC ...
-
bioRxiv - Microbiology 2021Quote: ... Primary human bronchial epithelial cells (BEpCs) from a 73 year-old female donor (donor 1) were purchased from Promocell (#C-12640). BEpCs from a 53 year-old male donor (donor 2 ...
-
bioRxiv - Cell Biology 2023Quote: ... the differentiated cells were divided into two growth medium as: (1) Microvascular endothelial growth medium (EGM-MV2, Cat# C-39226, PromoCell, GmbH, Germany) with added (TGF ...
-
Long-Range Electrostatic Interactions Significantly Modulate the Affinity of Dynein for MicrotubulesbioRxiv - Biophysics 2021Quote: ... We washed out excess antibody with BRB80 and then incubated the flow channel with 1% Pluronic F-127 (PK-CA707-59000, PromoCell Inc., Heidelberg, Germany) for 5 min to passivate the surface ...