Labshake search
Citations for PromoCell :
651 - 700 of 700 citations for Recombinant Human Programmed Cell Death 1 Ligand 2 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Physiology 2023Quote: ... were seeded at 4000 cells/cm2 density and cultured in the respective Fibroblast Growth Medium (C-23025, PromoCell).
-
bioRxiv - Cell Biology 2020Quote: ... Isolated TECs were seeded onto collagen-coated Petri dishes and cultured in renal epithelial cell medium (C-26130, PromoCell) supplemented with growth factors for TEC growth.
-
bioRxiv - Bioengineering 2021Quote: ... The epithelial cells were cultured and expanded in Airway Growth Medium (Airway Epithelial Growth Medium Kit, Promocell, C-21160) supplemented with 1% penicillin-streptomycin ...
-
bioRxiv - Developmental Biology 2021Quote: ... These cells were cultured in 15% FBS (GemCell, 100-500) and 5% Growth Medium Supplement Mix (PromoCell, C-39365) in Skeletal Muscle Cell Basal Medium (PromoCell ...
-
bioRxiv - Microbiology 2020Quote: ... The culture medium was changed to remove detached cells and treatments as indicated in endothelial basal medium (EBM, PromoCell) in the presence of 2 mM hydroxyurea to prevent cell proliferation ...
-
bioRxiv - Cancer Biology 2021Quote: Cell cultures tested for mycoplasma using a commercially available kit (PCR Mycoplasma Test Kit I/C, PromoCell, Heidelberg, Germany) shows absence of a positive band at ∼270bp ...
-
bioRxiv - Molecular Biology 2021Quote: ... Cells were resuspended and cultured in 0.5% gelatin-coated culture well (12-well format) in EGM2 medium (PromoCell, C30140) supplemented with 10% FBS ...
-
bioRxiv - Cell Biology 2021Quote: ... were seeded at a density 1.5 × 105 cells/well in 12-well plates and cultured in a myocyte growth medium (C-22170, PromoCell) containing 0.5 ng/ml epidermal growth factor ...
-
bioRxiv - Cell Biology 2022Quote: ... were purchased from PromoCell and cultured in 0.5% gelatin-coated dishes in in endothelial cell growth medium MV2 supplemented with C-39225 supplement mix (PromoCell) (Tornavaca et al. ...
-
bioRxiv - Cell Biology 2022Quote: ... University Medical Center of the Johannes Gutenberg University Mainz) and were cultured on 0.2% gelatin coating in Endothelial Cell Growth Medium (Cat# C-22010, PromoCell). HT-29 cells were purchased from ATCC (ATCC HTB-38 ...
-
bioRxiv - Microbiology 2023Quote: ... were obtained from Promocell and maintained as per the manufacturer’s instructions in 75 cm2 flasks at 37°C with 5% CO2 in endothelial cell growth medium (Promocell) with 100 U/mL penicillin ...
-
bioRxiv - Molecular Biology 2022Quote: ... and left for 3 days until 100 % confluency in full supplemented ECM MV2 cell media (Promocell, cat. #C-22022). 15 h before transfection was placed in serum-reduced ECM MV2 media (containing 10 % of originally cell growth supplement MV2) ...
-
bioRxiv - Cell Biology 2023Quote: ... Isolated TECs were seeded onto collagen-coated Petri dishes and cultured in renal epithelial cell medium (C-26130, PromoCell) supplemented with growth factors for TEC growth27.
-
bioRxiv - Cell Biology 2020Quote: ... into the vein for 20 minutes at 37°C and subsequently cultured in endothelial cell growth medium purchased from PromoCell supplemented with 1% penicillin/streptomycin and used up to passage 4 for all indicated experiments.
-
bioRxiv - Cell Biology 2021Quote: ... containing 5% heat-inactivated fetal calf serum (FCS) with supplements (endothelial cell growth supplement: epidermal growth factor, basic fibroblast growth factor, heparin, and hydrocortisone, from PromoCell). Primary human adipose microvascular endothelial cells (HAMEC ...
-
bioRxiv - Cancer Biology 2020Quote: ... Mycoplasma status was defined negative for all cell lines by PCR (PCR Mycoplasma Test Kit I/C, PromoCell GmbH, Germany) and cell lines were authenticated by Short Tandem Repeat genotyping (Eurofins Genomics ...
-
bioRxiv - Developmental Biology 2022Quote: ... Isolated HBE cells were maintained and expanded (one passage) in T75 flasks in hormone- and growth factor-supplemented airway epithelial cell growth medium (AEGM, ready-to-use; PromoCell) at 37 °C in a 5% CO2 incubator ...
-
bioRxiv - Cancer Biology 2020Quote: ... Kyse30 and FaDu cells were stably transfected with SLUG-Myc in the 141-pCAG-3SIP vector with MATra transfection reagent (PromoCell) using 1 μg/ mL puromycin (Sigma ...
-
bioRxiv - Immunology 2022Quote: ... Airway epithelial cells were taken using 2mm diameter cytology brushes from 3rd to 5th order bronchi and cultured in Airway Epithelial Cell medium (PromoCell) in submerged culture.
-
bioRxiv - Cell Biology 2022Quote: ... Once expanded, all endothelial cells (HUVEC, hCMEC/D3, HDLEC, HDBEC, and BOECs) were maintained in either ECGM or ECGM MV2 (Promocell) and endothelial phenotype regularly assessed by flow cytometry using anti-CD31-AF488 and anti-VE-cadherin-Biotin antibodies ...
-
bioRxiv - Microbiology 2022Quote: ... were cultured in Airway Epithelial Cell Growth Medium (basal medium supplemented with BPE, EGF, insulin, hydrocortisone, epinephrine, triiodo-L-thyronine, transferrin and retinoic acid; PromoCell) at 37 °C in a 5% CO2 atmosphere ...
-
bioRxiv - Microbiology 2023Quote: ... and cells were resuspended in 12 mL warm supplemented airway culture media (Airway Epithelial Cell Growth Basal Media, Promocell; supplemented with Growth Medium SupplementPack [#C-39160, Promocell] ...
-
bioRxiv - Microbiology 2023Quote: ... Primary cultures were established by thawing the cryovial in a 37°C water bath for 2 minutes and resuspending cells into warmed airway culture media (Airway Epithelial Cell Growth Basal Media, #C-21260, Promocell; supplemented with Growth Medium SupplementPack, #C-39160, Promocell), and plating at a density of 1.5 × 103 cells/cm2 into a T25 flask ...
-
bioRxiv - Cancer Biology 2023Quote: A single suspension containing 1000 cells per 100 μL was plated in 96-well ultra-low attachment plates (corning) in 3D tumorsphere media XF (PromoCell). Media was added to the well every 3 days and growth was monitored microscopically ...
-
bioRxiv - Cell Biology 2024Quote: ... Differentiation of the AB1190 myoblasts was induced when culture was near full confluence (>90%) by changing to Skeletal Muscle Cell Differentiation Medium (Promocell) and culturing for a further 12-14 days ...
-
bioRxiv - Microbiology 2024Quote: ... Trypsin was inactivated with 5ml of FBS and 5ml of cell-type specific culture medium (Astrocyte medium; AM, Sciencell. Pericyte medium; PM, Sciencell. BMEC medium; MV2, Promocell). Cell suspensions were spun at 120g for 5mins and resuspended in their respective medium prior to passage into a pre-coated flask ...
-
bioRxiv - Developmental Biology 2020Quote: ... were obtained from PromoCell (PC-C-12285 HCMEC-c) and cultured according to manufacturer’s instructions using Endothelial Cell Growth Medium MV (PromoCell, #PC-C-22020). For Smart-Seq2 analysis ...
-
bioRxiv - Bioengineering 2021Quote: ... and the pellet was resuspended in pre-warmed 10 mL Endothelial Cell Growth Medium (ECGM; C-22010, PromoCell GmbH, Heidelberg, Germany) with 10 mg/mL Gentamicin (mvEC expansion medium) ...
-
bioRxiv - Cell Biology 2020Quote: ... hSkMCs were seeded at a high density in the device and cultured in skeletal muscle cell growth medium (PromoCell, C-23060) for 2 days ...
-
bioRxiv - Bioengineering 2022Quote: ... were cultured in tissue culture flasks coated with 0.1% gelatin prior to cell seeding in endothelial growth media (PromoCell, C-C22121). RAW264.7 (ATCC ...
-
bioRxiv - Immunology 2021Quote: ... HUVECs were seeded at a density of 2.7 x 105 / cm2 on the endothelial side of the chip and grown in endothelial cell growth medium (PromoCell, Heidelberg, Germany). On the opposite chamber ...
-
bioRxiv - Cell Biology 2022Quote: ... Positive cells were seeded on mouse Collagen IV (Biotechne, 3410-010-02) coated plate in EGM2-MV medium (Promocell, C-22011) supplemented with 20% FBS (Thermo ...
-
bioRxiv - Cell Biology 2022Quote: ... were plated in collagen-coated plastic and cultured in cell-specific basal media supplemented with a growth media kit (PromoCell; Lonza) and 5% fetal bovine serum (FBS ...
-
bioRxiv - Microbiology 2022Quote: ... derived from a 26-year old adult were grown on collagen coated flasks using the Airway Epithelial Cell Growth Medium Kit (Promocell, Germany) supplemented with bovine pituitary extract (0.004ml/ml) ...
-
bioRxiv - Cancer Biology 2022Quote: ... Breast epithelial organoids were collected from the top of the 40µM filter in mammary epithelial cell growth medium with added supplements (PromoCell #C-21010). Isolated mammary epithelial organoids were snap frozen in liquid nitrogen for RNA extraction and RNA-sequencing or plated for in vitro studies.
-
bioRxiv - Microbiology 2023Quote: ... and the peripheral blood mononuclear cells (PBMCs) were isolated on the density gradient of lymphocyte separation medium 1077 (PromoCell, Heidelberg, Germany) using SepMate 50 ml tubes (StemCell Technologies ...
-
bioRxiv - Microbiology 2020Quote: ... were isolated as described before (Dehio et al., 1997) and cultured at 37°C with 5% CO2 in Endothelial Cell Growth Medium (ECGM, Promocell, C-22010) supplemented with Endothelial Cell Growth Medium SupplementMix (Promocell ...
-
bioRxiv - Genomics 2021Quote: ... HUVEC were seeded on 6-cm BTF glass plates coated with cross-linked gelatin and cultured to confluence (approximately 105 cells/cm2) in EC growth medium (Promocell, Heidelberg, Germany). The maximum flow of 18 dynes/cm2 was achieved by computer-controlled cone rotation in culture medium containing 3% polyvinylpyrrolidone (PVP ...
-
bioRxiv - Cancer Biology 2022Quote: ... were purchased from Cell Biologics (C57-6023) and subcultured on plates coated with attachment factor protein (Life Techonologies, S006110) in Endothelial Cell Growth Medium (Promocell, C-22111).
-
bioRxiv - Cell Biology 2022Quote: ... human PSC lines were differentiated to myogenic progenitor cells (MPCs) within 3∼4 weeks and subcultured in skeletal muscle growth medium (Promocell, C-23260). Cryopreserved MPCs were thawed in skeletal muscle growth medium (GM ...
-
bioRxiv - Cancer Biology 2022Quote: ... The pieces were weighed and placed on a 10cm dish with 10mL of basal (phenol red free, serum free, and supplement mix free) mammary epithelial cell growth media (PromoCell #C-21215) containing 0.1% BSA ...
-
bioRxiv - Cell Biology 2023Quote: 14 eEPC lines and 15 human adult endothelial cell (AEC) lines were cultured in endothelial growth medium (EGM-MV2, Cat# C-39226, Lot# 25 x 461M089, PromoCell, GmbH, Germany) on gelatin-coated plates ...
-
bioRxiv - Cell Biology 2023Quote: ... passage 8-10 immortalized myoblasts were plated into 15 cm tissue culture dishes (5×105 cells/dish) within the following growth medium: PromoCell Skeletal Muscle Growth Medium Kit (PromoCell C-23160) supplemented with 15% fetal bovine serum (FBS ...
-
bioRxiv - Cell Biology 2020Quote: ... The immortalized myoblast cell lines were maintained on 10 cm tissue culture dishes containing 7 mL of skeletal muscle cell growth medium (79 % PromoCell media (C-23160) supplemented with 15 % fetal bovine serum (Gibco ...
-
bioRxiv - Cancer Biology 2020Quote: ... The Emory Genomics Core authenticated cell lines for use and Mycoplasma testing was performed every 3 - 6 months using the Mycoplasma test kit (PromoCell, PK-CA91-1024). Cells were cultured in a humid incubator at 37°C with 5% CO2 ...
-
bioRxiv - Bioengineering 2022Quote: Primary human umbilical vein endothelial cells (HUVECs) that were isolated from the vein of the umbilical cord of a single donor (PromoCell GmbH, C-12200, Germany) were cultured in endothelial cell growth medium (ECGM ...
-
Tracer-based metabolomics for profiling nitric oxide metabolites in a 3D microvessel-on-a-chip modelbioRxiv - Cell Biology 2023Quote: Human coronary artery endothelial cells (HCAECs) (PomoCell, C-12221,) were resuspended in 10 ml fresh EGM MV2 medium with supplements (PromoCell,C-22022, C-39216) and cultured in T75 flasks (Nunc Easyflask ...
-
Long-Range Electrostatic Interactions Significantly Modulate the Affinity of Dynein for MicrotubulesbioRxiv - Biophysics 2021Quote: ... We washed out excess antibody with BRB80 and then incubated the flow channel with 1% Pluronic F-127 (PK-CA707-59000, PromoCell Inc., Heidelberg, Germany) for 5 min to passivate the surface ...
-
bioRxiv - Immunology 2020Quote: ... + 50 ug/mL fetuin + 10 ng/mL hEGF + 1 ng/mL bhFGF + 10 ug/mL insulin + 0.4 ug/mL dexamethasone (Promocell, Heidelberg, Germany, C-39360), and cells were maintained at 37 °C ...
-
bioRxiv - Microbiology 2022Quote: ... the supernatant was discarded and the cells were resuspended in 10 ml of adhesion medium for macrophages and fibroblasts (22.5 mL DMEM/F-12, 22.5 mL unsupplemented small airway epithelial cell growth medium, Promocell, 10% mL FBS and 160 U/ml DNase I). Cells were transferred to a T75 tissue culture flask and incubated at 37°C for 60-90 minutes to allow macrophages and fibroblasts to attach to the cell culture flask ...