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Citations for MP Biomedicals :
251 - 300 of 330 citations for Zika Virus Vero Cell Lysate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... The cells were then added to a 2 mL tube containing 0.1 mm Lysing Matrix B beads (MP Biomedicals) and were lysed using a Fastprep-24 bead beater(MP Biomedicals) ...
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bioRxiv - Microbiology 2020Quote: ... Cells were mechanically lysed three times for 30 sec with 0.1 mm silica beads (Lysing Matrix B, MP Biomedicals). The samples were then spun ...
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bioRxiv - Microbiology 2021Quote: ... Cells were lysed by bead beating (3 cycles, 40s each, 6.0 m/s) using a FastPrep homogenizer (MP Biomedicals). Material was centrifuged at 13,000×g for 2 min to remove unlysed cells and insoluble debris ...
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bioRxiv - Microbiology 2023Quote: ... The cell resuspension was lysed with 3 V of zirconia beads in a FastPrep-24TM 5G homogenizer (MP Biomedicals) for two cycles of 40 seconds at 6.5 m/sec with 1-minute rest in between ...
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bioRxiv - Microbiology 2024Quote: ... and resuspended cells were disrupted using Lysing Matrix B tubes in a FastPrep-24 homogenizer (MP Biomedicals, Irvine, CA) at 6 M/s ...
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bioRxiv - Microbiology 2019Quote: ... DNA was extracted from purified colonies using the phenol chloroform extraction method with the following modifications: glass beads (0.4mm) were used to lyse cells in a FastPrep-24 homogenizer (MP Biomedicals) with two cycles of 20 seconds at 4 m/s ...
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bioRxiv - Microbiology 2021Quote: ... Cells were resuspended in TBSI and broken using 0.1 mm silica spheres (Lysing Matrix B) and FastPrep Homogenizer (MP Biomedicals) in 12 cycles of 30 s ...
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bioRxiv - Molecular Biology 2020Quote: ... Cell pellets were lysed with 1 mL of acid-treated glass beads in a bead beater (FastPrep-5; MP Biomedicals) at level 7.5 for 15 s ...
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bioRxiv - Molecular Biology 2019Quote: ... 200 μl of 0.5 mm zirconia/silica beads were added to samples and cells were lysed using the FastPrep-24 (MP Biomedicals) on max speed (6.5 m/s) ...
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bioRxiv - Molecular Biology 2019Quote: ... 200 μl of 0.5 mm zirconia/silica beads were added to samples and cells were lysed using the FastPrep-24 (MP Biomedicals) on max speed (6.5 m/s) ...
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bioRxiv - Biochemistry 2019Quote: ... 10 to 25 mL cultures of exponentially growing cells were homogenized by glass bead-beating in a FastPrep (MP Biomedicals). Proteins extracted using either standard lysis buffer (WEB ...
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bioRxiv - Microbiology 2020Quote: ... then adding 90 µL to each well such that the final concentration of the bile acid after the addition of cells (10 µL) ranged from 0.04 mM to 10 mM for CA (102897, MP Biomedicals), and 0.01 mM to 2.5 mM for DCA (D6750 ...
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bioRxiv - Genetics 2020Quote: ... 0.3 g glass beads were added and the cells in the samples were lysed using a FastPrep-24 (MP Biomedicals). 100 µL of 3x SDS loading buffer was added ...
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bioRxiv - Microbiology 2020Quote: ... using the Qiagen AllPrep DNA/RNA Mini Kit according to the manufacturer’s instructions with cell lysis by a bead beating step in Lysing Matrix E tubes (MP Biomedicals) in a FastPrep homogenizer (MP Biomedicals ...
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bioRxiv - Immunology 2020Quote: ... and peripheral mononuclear cells (PBMCs) were isolated using standard density gradient centrifugation over lymphocyte separation media (MP Biomedicals, Solon, OH) and any contaminating red blood cells were lysed using hypotonic ammonium chloride solution ...
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bioRxiv - Microbiology 2021Quote: ... Cell lines were routinely assessed for Mycoplasma contamination by PCR using a Myco-Sniff Mycoplasma PCR Detection Kit (MP Biomedicals). All parasite lines used and generated in this study are listed in Table S1.
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bioRxiv - Genetics 2022Quote: ... Yeast cells were grown in YPD medium (2%) or in SC medium [containing yeast nitrogen base (1.7 g/liter; MP Biomedicals), ammonium sulfate (5 g/liter ...
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bioRxiv - Immunology 2022Quote: ... MDCK cells expressing IAV-NS1 (MDCK-NS1 cells) were infected with IAVdelNS1 in EMEM containing 0.35% bovine serum albumin (BSA, MP Biomedicals), 4 mM L-glutamine ...
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bioRxiv - Immunology 2023Quote: ... Cells were subsequently washed with PBS and resuspended in serum- free RPMI with 3ug/mL Beta-2-microglobulin (MP Biomedicals) and the indicated concentration of peptide for 3 hours at room temperature followed by 3 hours at 37C in 5% CO2 ...
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bioRxiv - Microbiology 2023Quote: Genomic DNA was extracted from cell pellets using the Fast DNA SPIN Kit for Soil (MP Biomedicals, Santa Ana, CA) according to the manufacturers’ protocol with a minor modification in which the gDNA was eluted in 1.0 mM Tris with 0.01 mM EDTA at pH 8 ...
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bioRxiv - Biochemistry 2023Quote: ... Resuspended cells were placed in tubes containing 0.1-mm silica beads (Lysing matrix B tubes, catalog no. 6911-100; MP Biomedicals) and lysed in a bead beater (Bead Ruptor 24 Elite ...
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bioRxiv - Immunology 2024Quote: ... Primary peripheral blood mononuclear cells (PBMCs) were isolated from human blood by density centrifugation using Lymphocyte Separation Medium (MP Biomedicals). Human monocytes were negatively selected from PBMCs using the monocytes Isolation Kit (Miltenyi Biotec) ...
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bioRxiv - Microbiology 2023Quote: Genomic DNA was extracted from cell pellets using the Fast DNA SPIN Kit for Soil (MP Biomedicals, Santa Ana, CA) as previously published (61) ...
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bioRxiv - Immunology 2024Quote: Primary peripheral blood mononuclear cells (PBMCs) were isolated from human blood by density centrifugation using Lymphocyte Separation Medium (MP Biomedicals). CD4+ T cells were negatively selected from PBMCs using CD4+ T cell isolation kit (Miltenyi Biotec ...
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bioRxiv - Microbiology 2023Quote: ... the cells were assessed for complement deposition by staining with goat anti-guinea pig C3-FITC (MP Biomedicals; catalog #0855385). After fixing ...
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bioRxiv - Microbiology 2024Quote: ... Total RNA are extracted after cell wall mechanical lysis with 0.1 µm beads (Precellys Evolution, Bertin Technologies) in RNApro reagent (MP Biomedicals), and purified by chloroform extraction and ethanol precipitation ...
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bioRxiv - Microbiology 2024Quote: ... 1 µL of cells were then spotted onto PBS agarose (1%) pads in 10-well multitest microscope slides (MP Biomedicals). Microscopy acquisition was performed using a Zeiss M1 upright microscope with a 100x oil-immersion objective ...
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bioRxiv - Microbiology 2024Quote: ... Cells were cultured overnight from frozen glycerol stock in Luria-Bertani (LB) broth and 10 µg/mL tetracycline (MP Biomedicals).
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bioRxiv - Cell Biology 2020Quote: ... a 50-μL aliquot of cells from sporulation culture was pelleted and resuspended in 1 mg/mL Zymolyase-20T (#320921, MP Biomedicals) dissolved in water and incubated at 30°C for 10 minutes.
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bioRxiv - Cell Biology 2020Quote: Yeast cells were grown either in rich medium (YPD) or in enriched complete synthetic medium (2x final concentration of CSM, MP Biomedicals) supplemented with 2% glucose or 2% galactose (wt/vol) ...
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bioRxiv - Developmental Biology 2021Quote: ... Membrane potentials (Vm) were monitored using the perforated whole-cell patch-clamp method with β-escin (MP Biomedicals, Santa Ana, CA) as the perforating agent (6 to 8 μM) ...
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bioRxiv - Cell Biology 2022Quote: ... The following day the media on the cells were replaced with media containing a combination of manganese chloride (MP Biomedicals, 155334) and Actinonin (Sigma ...
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bioRxiv - Microbiology 2019Quote: ... A 0.5 mL aliquot of the cell suspension was transferred to a FastPrep Lysing Matrix B (MP Biomedicals, Santa Ana, CA) tube containing 0.5 mL of PBS (Invitrogen ...
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bioRxiv - Microbiology 2019Quote: For protein extraction, cells were lysed (in 1 % sodium deoxycholate, 50 mM triethylammonium bicarbonate) using the FastPrep-96 Instrument (MP Biomedicals) for 1 min at 1600 rpm and the All FastDNA-96 (MP Biomedicals ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were transduced with lentiviral particles at MOI of 10 in a 150 μL of cell culture media supplemented with 10 μg/mL of protamine sulfate (#02194729, MP Biomedicals) and saved as ReN-CAG-BirA ...
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bioRxiv - Systems Biology 2020Quote: ... peripheral blood mononuclear cells (PBMCs) were isolated by density gradient centrifugation using LSM Lymphocyte Separation Medium (MP Biomedicals, Santa Ana, CA). PBMCs were enriched for total monocytes using the Pan Monocyte Isolation Kit (Miltenyi Biotech ...
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bioRxiv - Microbiology 2022Quote: ... 600 μl of Trizol reagent was added and cells were then mechanically disrupted using a MP Biomedicals FastPrep Instrument and Lysing Matrix B tubes (MP Biomedicals). Black carbon particles were removed by centrifugation at 12 ...
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bioRxiv - Microbiology 2021Quote: ... pPol-I firefly Luc (NP gene; 100ng) and SV40 Renilla (50ng) reporter into HEK293T cells using polyethylenimine reagent (PEI, MP Biomedicals) at a ratio of 1:5 (DNA:PEI) ...
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bioRxiv - Immunology 2020Quote: Peripheral blood mononuclear cells (PBMC) were isolated from heparinized blood samples of human healthy donors using Ficoll-Paque density centrifugation (MP biomedicals), frozen and then stored in liquid nitrogen ...
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bioRxiv - Immunology 2020Quote: ... resuspended at 5×108 bacteria /mL and lysed using a FastPrep-24 Tissue and Cell Homogenizer in Lysing Matrix B tubes (MP Biomedicals) with a setting of 4.0 m/s for 20s ...
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bioRxiv - Immunology 2022Quote: ... Transduction was carried out two times for 24 hours and transduced cells were selected with appropriate antibiotics (5 μg/ml blasticidin, MP Biomedicals, Santa Ana ...
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bioRxiv - Molecular Biology 2022Quote: ... were added to the pellet and bead-beaten at 6m/s for 30 seconds using an MP Fast Prep-24 Tissue and Cell Homogenizer (MP Biomedicals).
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bioRxiv - Microbiology 2022Quote: ... Harvested cells suspended in 100 μl of lysis buffer (74) were mechanically disrupted in FastPrep Lysis Matrix A tubes (MP Biomedicals) at 30 Hz for 10 min ...
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bioRxiv - Molecular Biology 2022Quote: ... and lysis was carried out by 6 x 1min maximum speed cycles on a FastPrep-24™ cell homogeniser (MP Biomedicals), with the samples being returned to ice for 5min between each cycle ...
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bioRxiv - Cell Biology 2023Quote: ... 10 μM ZnSO4 and 2 mM PMSF and cells were lysed by glass bead lysis in the FastPrep-24 (MP Biomedicals). Pho8 activity was monitored by incubation of 200 μl lysate with 50 μl buffer 250 mM Tris–HCl ...
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bioRxiv - Microbiology 2023Quote: ... Cells were disrupted by bead beating for 40 seconds at 6.5m/s in a FastPrep-24 instrument (MP Biomedicals, United states). The supernatant was transferred to an Eppendorf tube and chloroform (Sigma-Aldrich ...
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bioRxiv - Microbiology 2022Quote: ... the larvae/embryos were mixed with 100 μL PBS in 1.5 mL tubes and homogenized with 3 cycles of 15 s at 6,500 rpm with rest intervals of 60 s by FastPrepTM 24-5G tissue-cell homogenizer (MP Biomedicals, USA). The homogenates were clarified by centrifugation at 9,000 × g for 5 min and the supernatants were used as the virus suspension for RT-qPCR analysis or further investigation (passaging of viruses by being injected to new zebrafish embryos ...
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bioRxiv - Cell Biology 2023Quote: ... 200 µl of acid-washed beads were added and cells were mechanically lysed twice in a FastPrep device (6m/sec for 40 seconds; MP Biomedicals), with a 5-minute incubation on ice in between ...
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bioRxiv - Microbiology 2023Quote: ... One mL of the culture was centrifuged at 10,000 × g for 10 minutes and the cell pellet was used for DNA extraction using the FastDNA SPIN Kit (MP Biomedicals).
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bioRxiv - Cell Biology 2023Quote: ... 300 µl acid-washed glass beads were added and cells were mechanically lysed twice with a FastPrep device (6m/sec for 40 seconds; MP Biomedicals) with a 5-minute incubation on ice in between ...