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Citations for MP Biomedicals :
51 - 100 of 372 citations for Yeast Expression Cloning kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... cultures were grown in low fluorescence media (1.7 g/L Yeast Nitrogen Base without ammonium sulfate, without folic acid, without riboflavin [MP Biomedicals # 114030512] ...
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bioRxiv - Genomics 2021Quote: ... Composition of the nitrogen-depleted media was as follows: 0.67% Yeast Nitrogen Base without amino acids and ammonium sulfate (MP Biomedicals), 2% D-glucose ...
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bioRxiv - Genetics 2022Quote: ... Yeast cells were grown in YPD medium (2%) or in SC medium [containing yeast nitrogen base (1.7 g/liter; MP Biomedicals), ammonium sulfate (5 g/liter ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... SY media contained 10 g of Grade J3 agar (Gelita Australia Pty Ltd.; order code: A-181017, 100 g of autolysed Brewer’s Yeast (MP Biomedicals; order code ...
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bioRxiv - Cell Biology 2022Quote: Yeast cells were grown in liquid to exponential growth phase in SC medium (1.7 g/l yeast nitrogen base (#1545, CONDA) 5 g/l ammonium sulfate (#4808211, MP Biomedicals), 20 g/l glucose (#1422 ...
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bioRxiv - Biochemistry 2023Quote: ... supplemented with 2 % glucose or 2 % galactose as the carbon source or in synthetic medium (0.17 % yeast nitrogen base (MP Biomedicals), 0.5 % (NH4)2SO4 ...
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bioRxiv - Cell Biology 2021Quote: ... pombe cultures were grown at 30 °C either in rich medium (yeast extract supplemented with 0.15 g/L adenine; YEA) or in Edinburgh minimal medium (EMM, MP Biomedicals, 4110012) supplemented with 0.2 g/L leucine ...
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bioRxiv - Microbiology 2022Quote: ... cerevisiae S288C was used as the heterologous expression host to probe the roles of host proteins in the function of OspB and was routinely cultured at 30 °C in yeast extract-peptone-dextrose (YPD) broth or in synthetic selective media (MP Biomedicals) lacking histidine ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... yeast were selected for on plates lacking leucine and grown in SCD media lacking leucine (5 g/L ammonium sulfate, 1.7 g/L yeast nitrogen base without amino acids (MP Biomedicals; 4510522), 1 NaOH pellet (VWR ...
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bioRxiv - Biochemistry 2024Quote: ... cerevisiae transformed with WT and mutant construct were grown in yeast synthetic drop-out medium supplemented with Uracil (CSM-URA; MP Biomedicals) to an OD600 of 0.8 and expression was induced with 2% (w/v ...
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bioRxiv - Bioengineering 2021Quote: ... Total proteome was obtained using the Y-PER reagent with yeast cells in a glass beads-containing tube and by disruption of the cells in a FastPrep-24 homogenizer (MP Biomedicals, USA). For cell disruption ...
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bioRxiv - Genetics 2020Quote: ... Specific nitrogen source and nitrogen depletion media was prepared immediately before use from 2X S+Uri (3.4 g/L Yeast Nitrogen Base without Amino Acids or ammonium sulfate (MP Biomedicals, #4027-032), 200 µg/mL uridine) ...
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bioRxiv - Microbiology 2022Quote: ... 10 μg ml-1 uracil and 0.73 g l-1 yeast methionine/uracil drop-out medium (CSM-Met-Ura, MP Biomedicals, USA, Solon) and lacked NZ-amine ...
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bioRxiv - Evolutionary Biology 2021Quote: ... diet was created using 50 g/l of sucrose (Bundaberg Sugar, Melbourne Distributors) and 100 g/l whole yeast autolysate (MP Biomedicals, LLC, #903312), previously described in Mair et al ...
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bioRxiv - Cell Biology 2023Quote: Yeast cell pellets (30 OD) were snap frozen and lysed by bead disruption using a FastPrep-24 cell homogenizer (MP Biomedicals; cat# 116004500) for 20s in NP-40 lysis buffer (6 mM Na2HPO4 ...
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bioRxiv - Cancer Biology 2024Quote: ... The expression of PLXNB2 was induced by addition of 1 μg/ml Dox (MP Biomedicals) to the culture medium.
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bioRxiv - Evolutionary Biology 2023Quote: For each experiment we put approximately 200 age-matched parental beetles on fresh beetle media (whole-wheat flour from MP Biomedicals and 5% yeast) to lay eggs for 24 hours and then we removed the parents onto new media ...
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bioRxiv - Biophysics 2021Quote: ... Strains carrying GEM expression vectors were grown in EMM3S – Edinburgh Minimum Media (#4110-32, MP Biomedicals) supplemented with 0.225 g/L of uracil ...
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bioRxiv - Cancer Biology 2021Quote: ... Expression of DN-Maml1 can be genetically induced following treatment with ß-napthoflavone (BNF, MP Biomedicals Cat# 156738) and tamoxifen (TAM ...
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bioRxiv - Molecular Biology 2024Quote: ... and ectopic protein expression in Flp-In T-REx HeLa cells was induced with 1 μg/mL doxycycline (MP Biomedicals, 195044, Dox) minimum three hours post-seeding ...
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bioRxiv - Biophysics 2023Quote: ... pombe strains carrying GEMs expression plasmids were grown in similar conditions in EMM3S – Edinburgh Minimum media (#4110-32, MP Biomedicals, Burlingame CA, USA) supplemented with 0.225 g/L of uracil ...
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bioRxiv - Microbiology 2023Quote: ... DNA was extracted using a DNA extraction kit (FastDNA™ SPIN Kit for Soil, MP Biomedicals). DNA concentration of each extract was determined fluorimetrically following the Picogreen assay (Quant-iT™ PicoGreen® dsDNA Reagent ...
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bioRxiv - Zoology 2021Quote: ... Plasma samples were assayed in duplicate using a corticosterone radioimmunoassay kit (MP Biomedicals CortiCote RIA kit, #06B256440); further details of this plasma assay are published in Holekamp and Smale (1998).
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bioRxiv - Microbiology 2023Quote: ... The DNA was extracted with the FastDNA™ SPIN Kit for Soil DNA isolation kit (MP Biomedicals) according to the protocol.
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bioRxiv - Plant Biology 2020Quote: ... purified (Geneclean turbo kit, MP Biomedicals, USA) and sent for sequencing (Eurofins Genomics ...
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bioRxiv - Microbiology 2021Quote: ... using a FastDNA SPIN Kit (MP Biomedicals) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... or FastRNA PRO BLUE kit (MP Biomedicals) (see (36 ...
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bioRxiv - Microbiology 2021Quote: ... FastDNA™ Spin Kit (MP Biomedicals™) was used to extract DNA from 0.5 g of sediment and 0.5 L of water (0.22 μm PES filter) ...
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bioRxiv - Microbiology 2019Quote: ... using a FastDNA SPIN Kit (MP Biomedicals) following the manufacturer’s instructions ...
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bioRxiv - Genomics 2024Quote: ... in a FastPrep-24 kit (MP Biomedicals) (6.5 m/s for 15 s) ...
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bioRxiv - Plant Biology 2019Quote: Metagenomic DNA (Mg-DNA) was isolated from the samples using the kit FastDNA™ SPIN Kit for Soil (MP Biomedicals). In brief ...
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bioRxiv - Microbiology 2022Quote: ... and FastDNA Spin Kit for Soil (MP Biomedicals), following the instructions provided by the manufacturer ...
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bioRxiv - Developmental Biology 2023Quote: ... The samples were centrifuged at 3,600 g for 15 min at 4°C and the plasma was stored in the freezer at -20°C for subsequent measurements by a solid-phase RIA kit following the manufacture’s protocol (ImmuchemTM Double Antibody Progesterone Kit; Cat. 07e170105, MP Biomedicals, NY, USA). The intra-assay CV was 4.37% ...
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bioRxiv - Evolutionary Biology 2022Quote: ... or the FastDNA kit (MP Biomedicals, Irvine, California, USA). Amplification of waxy followed Levin et al ...
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bioRxiv - Neuroscience 2019Quote: ... Corticosterone double-antibody radioimmunoassay kits (MP biomedicals, Solon, OH) were used according to manufacturer’s instructions and the Inter- and intra-assay coefficients of variation were < 10%.
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bioRxiv - Bioengineering 2020Quote: ... FastDNA™ SPIN Kit (MP Biomedicals, Irvine, CA, USA) was used for DNA extraction according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... then with the GeneClean Turbo kit (MP Biomedicals, France) according to the manufacturer’s protocol (30) ...
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bioRxiv - Microbiology 2022Quote: ... using the FastRNA Pro Soil-Direct Kit (MP Biomedicals) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... A FastDNA SPIN Kit (MP Biomedicals, Solon, OH, USA) was used to extract community DNA following the manufacturer’s protocol with extra wash steps to maximize removal of environmental contaminants ...
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bioRxiv - Bioengineering 2023Quote: ... using FastDNA SPIN Kits for Soil (MP Biomedicals, USA). The pair of forward 27F (5’-AGAGTTTGATCCTGGCTCAG-3’ ...
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bioRxiv - Neuroscience 2022Quote: ... Corticosterone levels were determined by ImmuChem Double Antibody Corticosterone 125I-corticosterone Radioimmunoassay Kit for Rats and Mice according to kit instructions (MP Biomedicals, Santa Ana CA). A standard curve was generated for each run of RIA for 0-1000ng/ml concentration ...
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bioRxiv - Plant Biology 2022Quote: ... thus we used FastRNA PRO™ BLUE KIT (MP Biomedicals), which involves mechanical cell lysis ...
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bioRxiv - Bioengineering 2021Quote: ... DNA was purified using GENECLEAN® Turbo Kit (MP Biomedicals). The purified template DNA was mixed with an in vitro transcription mixture [T7 RNA polymerase (NEB) ...
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bioRxiv - Microbiology 2021Quote: ... DNA was extracted using the FastDNA SPIN Kit (MP Biomedicals) (48)
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bioRxiv - Bioengineering 2020Quote: The commercial kit FastRNA Pro™ Soil-Direct (MP Biomedicals) was used to extract the total RNA following the manufacturer’s specifications ...
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bioRxiv - Evolutionary Biology 2020Quote: ... mixed and purified using GENECLEAN® Turbo Kit (MP Biomedicals).
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bioRxiv - Microbiology 2020Quote: ... using FastDNA spin kit for DNA extraction (MP Biomedicals, USA), according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... monocytogenes was extracted using the FastDNA™ Kit (MP Biomedicals) and libraries for sequencing were prepared using the Illumina Nextera DNA kit ...
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bioRxiv - Microbiology 2020Quote: ... RNA was extracted using a FastRNA Blue Kit (MP Biomedicals) and ethanol-precipitated ...
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bioRxiv - Microbiology 2022Quote: ... using the FastDNA™ Spin Kit for Soil (MP Biomedicals). We used 2× Luna® Universal qPCR Master Mix (New England Biolabs [NEB] ...