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Citations for MP Biomedicals :
301 - 350 of 367 citations for Urothelial Cell Culture Supplements since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... using the Qiagen AllPrep DNA/RNA Mini Kit according to the manufacturer’s instructions with cell lysis by a bead beating step in Lysing Matrix E tubes (MP Biomedicals) in a FastPrep homogenizer (MP Biomedicals ...
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bioRxiv - Immunology 2020Quote: ... and peripheral mononuclear cells (PBMCs) were isolated using standard density gradient centrifugation over lymphocyte separation media (MP Biomedicals, Solon, OH) and any contaminating red blood cells were lysed using hypotonic ammonium chloride solution ...
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bioRxiv - Microbiology 2021Quote: ... Cell lines were routinely assessed for Mycoplasma contamination by PCR using a Myco-Sniff Mycoplasma PCR Detection Kit (MP Biomedicals). All parasite lines used and generated in this study are listed in Table S1.
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bioRxiv - Genetics 2022Quote: ... Yeast cells were grown in YPD medium (2%) or in SC medium [containing yeast nitrogen base (1.7 g/liter; MP Biomedicals), ammonium sulfate (5 g/liter ...
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bioRxiv - Immunology 2023Quote: ... Cells were subsequently washed with PBS and resuspended in serum- free RPMI with 3ug/mL Beta-2-microglobulin (MP Biomedicals) and the indicated concentration of peptide for 3 hours at room temperature followed by 3 hours at 37C in 5% CO2 ...
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bioRxiv - Immunology 2022Quote: ... MDCK cells expressing IAV-NS1 (MDCK-NS1 cells) were infected with IAVdelNS1 in EMEM containing 0.35% bovine serum albumin (BSA, MP Biomedicals), 4 mM L-glutamine ...
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bioRxiv - Microbiology 2023Quote: Genomic DNA was extracted from cell pellets using the Fast DNA SPIN Kit for Soil (MP Biomedicals, Santa Ana, CA) according to the manufacturers’ protocol with a minor modification in which the gDNA was eluted in 1.0 mM Tris with 0.01 mM EDTA at pH 8 ...
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bioRxiv - Biochemistry 2023Quote: ... Resuspended cells were placed in tubes containing 0.1-mm silica beads (Lysing matrix B tubes, catalog no. 6911-100; MP Biomedicals) and lysed in a bead beater (Bead Ruptor 24 Elite ...
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bioRxiv - Microbiology 2023Quote: Genomic DNA was extracted from cell pellets using the Fast DNA SPIN Kit for Soil (MP Biomedicals, Santa Ana, CA) as previously published (61) ...
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bioRxiv - Microbiology 2024Quote: ... Total RNA are extracted after cell wall mechanical lysis with 0.1 µm beads (Precellys Evolution, Bertin Technologies) in RNApro reagent (MP Biomedicals), and purified by chloroform extraction and ethanol precipitation ...
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bioRxiv - Microbiology 2024Quote: ... 1 µL of cells were then spotted onto PBS agarose (1%) pads in 10-well multitest microscope slides (MP Biomedicals). Microscopy acquisition was performed using a Zeiss M1 upright microscope with a 100x oil-immersion objective ...
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bioRxiv - Immunology 2024Quote: Primary peripheral blood mononuclear cells (PBMCs) were isolated from human blood by density centrifugation using Lymphocyte Separation Medium (MP Biomedicals). CD4+ T cells were negatively selected from PBMCs using CD4+ T cell isolation kit (Miltenyi Biotec ...
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bioRxiv - Immunology 2024Quote: ... Primary peripheral blood mononuclear cells (PBMCs) were isolated from human blood by density centrifugation using Lymphocyte Separation Medium (MP Biomedicals). Human monocytes were negatively selected from PBMCs using the monocytes Isolation Kit (Miltenyi Biotec) ...
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bioRxiv - Microbiology 2024Quote: ... Cells were cultured overnight from frozen glycerol stock in Luria-Bertani (LB) broth and 10 µg/mL tetracycline (MP Biomedicals).
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bioRxiv - Microbiology 2023Quote: ... the cells were assessed for complement deposition by staining with goat anti-guinea pig C3-FITC (MP Biomedicals; catalog #0855385). After fixing ...
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bioRxiv - Cell Biology 2020Quote: Yeast cells were grown either in rich medium (YPD) or in enriched complete synthetic medium (2x final concentration of CSM, MP Biomedicals) supplemented with 2% glucose or 2% galactose (wt/vol) ...
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bioRxiv - Developmental Biology 2021Quote: ... Membrane potentials (Vm) were monitored using the perforated whole-cell patch-clamp method with β-escin (MP Biomedicals, Santa Ana, CA) as the perforating agent (6 to 8 μM) ...
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bioRxiv - Cell Biology 2022Quote: ... The following day the media on the cells were replaced with media containing a combination of manganese chloride (MP Biomedicals, 155334) and Actinonin (Sigma ...
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bioRxiv - Microbiology 2019Quote: ... A 0.5 mL aliquot of the cell suspension was transferred to a FastPrep Lysing Matrix B (MP Biomedicals, Santa Ana, CA) tube containing 0.5 mL of PBS (Invitrogen ...
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bioRxiv - Microbiology 2019Quote: For protein extraction, cells were lysed (in 1 % sodium deoxycholate, 50 mM triethylammonium bicarbonate) using the FastPrep-96 Instrument (MP Biomedicals) for 1 min at 1600 rpm and the All FastDNA-96 (MP Biomedicals ...
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bioRxiv - Systems Biology 2020Quote: ... peripheral blood mononuclear cells (PBMCs) were isolated by density gradient centrifugation using LSM Lymphocyte Separation Medium (MP Biomedicals, Santa Ana, CA). PBMCs were enriched for total monocytes using the Pan Monocyte Isolation Kit (Miltenyi Biotech ...
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bioRxiv - Microbiology 2022Quote: ... 600 μl of Trizol reagent was added and cells were then mechanically disrupted using a MP Biomedicals FastPrep Instrument and Lysing Matrix B tubes (MP Biomedicals). Black carbon particles were removed by centrifugation at 12 ...
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bioRxiv - Microbiology 2021Quote: ... pPol-I firefly Luc (NP gene; 100ng) and SV40 Renilla (50ng) reporter into HEK293T cells using polyethylenimine reagent (PEI, MP Biomedicals) at a ratio of 1:5 (DNA:PEI) ...
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bioRxiv - Microbiology 2021Quote: ... 1 x protease inhibitor (HALT cocktail, ThermoScientific) after the lysate was prepared following cell lysis via bead-beating (Fastprep Pro, MP Biomedicals). Immunoprecipitations were performed on 2-3 biological replicates.
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bioRxiv - Immunology 2020Quote: Peripheral blood mononuclear cells (PBMC) were isolated from heparinized blood samples of human healthy donors using Ficoll-Paque density centrifugation (MP biomedicals), frozen and then stored in liquid nitrogen ...
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bioRxiv - Immunology 2020Quote: ... resuspended at 5×108 bacteria /mL and lysed using a FastPrep-24 Tissue and Cell Homogenizer in Lysing Matrix B tubes (MP Biomedicals) with a setting of 4.0 m/s for 20s ...
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bioRxiv - Immunology 2022Quote: ... Transduction was carried out two times for 24 hours and transduced cells were selected with appropriate antibiotics (5 μg/ml blasticidin, MP Biomedicals, Santa Ana ...
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bioRxiv - Molecular Biology 2022Quote: ... were added to the pellet and bead-beaten at 6m/s for 30 seconds using an MP Fast Prep-24 Tissue and Cell Homogenizer (MP Biomedicals).
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bioRxiv - Microbiology 2022Quote: ... Harvested cells suspended in 100 μl of lysis buffer (74) were mechanically disrupted in FastPrep Lysis Matrix A tubes (MP Biomedicals) at 30 Hz for 10 min ...
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bioRxiv - Molecular Biology 2022Quote: ... and lysis was carried out by 6 x 1min maximum speed cycles on a FastPrep-24™ cell homogeniser (MP Biomedicals), with the samples being returned to ice for 5min between each cycle ...
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bioRxiv - Microbiology 2022Quote: ... the larvae/embryos were mixed with 100 μL PBS in 1.5 mL tubes and homogenized with 3 cycles of 15 s at 6,500 rpm with rest intervals of 60 s by FastPrepTM 24-5G tissue-cell homogenizer (MP Biomedicals, USA). The homogenates were clarified by centrifugation at 9,000 × g for 5 min and the supernatants were used as the virus suspension for RT-qPCR analysis or further investigation (passaging of viruses by being injected to new zebrafish embryos ...
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bioRxiv - Cell Biology 2023Quote: ... 10 μM ZnSO4 and 2 mM PMSF and cells were lysed by glass bead lysis in the FastPrep-24 (MP Biomedicals). Pho8 activity was monitored by incubation of 200 μl lysate with 50 μl buffer 250 mM Tris–HCl ...
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bioRxiv - Microbiology 2023Quote: ... Cells were disrupted by bead beating for 40 seconds at 6.5m/s in a FastPrep-24 instrument (MP Biomedicals, United states). The supernatant was transferred to an Eppendorf tube and chloroform (Sigma-Aldrich ...
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bioRxiv - Immunology 2023Quote: ... propagation of the virus was carried out in Vero (African green monkey kidney epithelial – ATCC: CCL-81) cells cultured in MEM (MP Biomedicals), supplemented with 1 µg/mL TPCK-Trypsin (Trypsin-Worthington) ...
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bioRxiv - Cell Biology 2023Quote: ... 500 µl acid-washed glass beads were added and cells were mechanically lysed twice in a FastPrep device (6m/sec for 40 seconds; MP Biomedicals), with a 5-minute incubation on ice in between ...
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bioRxiv - Microbiology 2023Quote: ... One mL of the culture was centrifuged at 10,000 × g for 10 minutes and the cell pellet was used for DNA extraction using the FastDNA SPIN Kit (MP Biomedicals).
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bioRxiv - Cell Biology 2023Quote: ... 300 µl acid-washed glass beads were added and cells were mechanically lysed twice with a FastPrep device (6m/sec for 40 seconds; MP Biomedicals) with a 5-minute incubation on ice in between ...
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bioRxiv - Cancer Biology 2023Quote: ... Stably transfected or lentivirally transduced cells were selected and maintained in the same medium supplemented with 0.5 to 1 µg/mL puromycin (MP Biomedicals 194539) or 4 µg/mL blasticidin (US Biological Life Sciences B2104-30) ...
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bioRxiv - Cell Biology 2023Quote: ... 200 µl of acid-washed beads were added and cells were mechanically lysed twice in a FastPrep device (6m/sec for 40 seconds; MP Biomedicals), with a 5-minute incubation on ice in between ...
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bioRxiv - Microbiology 2023Quote: ... Only the initial cell lysis step was carried out in a FastPrep-24 instrument in lysing matrix A tubes (both MP Biomedicals) under different settings for the supernatant and cellular fraction samples ...
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bioRxiv - Immunology 2023Quote: ... DMEM (for labeling HeLa cells) or RPMI minus leucine (RPMI without L-leucine, L-glutamine, and sodium pyruvate from MP Biomedicals, supplemented with Glutamax and 1mM sodium pyruvate ...
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bioRxiv - Immunology 2024Quote: To further label viral RNA, cells were washed twice in Hybridization Wash Buffer (HWB) (2X SSC, 10% formamide (MP Biomedicals FORMD002), 0.2 mg/mL Bovine Seric Albumin (BSA ...
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bioRxiv - Molecular Biology 2024Quote: ... cells were distributed to two screw-cap microtubes containing 300 µl glass beads and lysed in a FastPrep instrument (MP Biomedicals) at 3 x 45 s 6 m/s ...
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bioRxiv - Cancer Biology 2022Quote: ... Viral infections were carried out over 72 hours and transduced cells were selected by their resistance to 2 μg/mL puromycin (MP Biomedicals 0219453910).
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bioRxiv - Neuroscience 2021Quote: ... 50,000/well freshly dispersed anterior pituitary cells were plated on poly-L-lysine-coated 8-well multitest slides (MP Biomedicals, Aurora, OH), bathed in Earle’s medium 199 supplemented with 2.2 g/L sodium bicarbonate ...
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bioRxiv - Cell Biology 2021Quote: ... for SA14-14-2 endogenous pools of cysteine and methionine were depleted by treating cells for 2 h with Cys− Met− MEM (MP Biomedicals #1641454) containing 7.5 μg/ml Actinomycin-D (AMD ...
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bioRxiv - Genetics 2022Quote: ... lysis of cells and subsequent isolation of total RNA was carried out using the lysing matrix B/FastPrep® sample preparation system (MP Biomedicals) and the miRNeasy Mini Kit (Qiagen) ...
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bioRxiv - Microbiology 2020Quote: The zebrafish larvae collected in Lysing Matrix Y tubes were homogenized with 3 cycles of 15 s at 6,500 RPM with rest intervals of 60 s by FastPrep™ 24-5G tissue-cell homogenizer (MP Biomedicals, USA). The homogenates were clarified by centrifugation at 9,000 × g for 5 min and RNA was extracted using RNeasy Mini Kit (Qiagen ...
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bioRxiv - Microbiology 2020Quote: The cells were fixed in 4% paraformaldehyde (Wako) and then permeabilized using 0.3% Triton X-100 (MP Biomedicals; Santa Ana, CA, USA). After blocking the cells by incubation in PBS containing 1% bovine serum albumin (BSA ...
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bioRxiv - Cell Biology 2022Quote: ... cells were resuspended in 20 µL of [1 mg/mL] 100T zymolyase (cat. no. 08320932, MP Biomedicals or cat. no. 320932, VWR) and incubated at room temperature for 6-7 minutes ...