Labshake search
Citations for MP Biomedicals :
101 - 150 of 175 citations for Surfactant Protein D SFTPD Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... Fc receptors were blocked for 30 min with Protein A (MP Biomedicals, OH) in Stain Buffer (554656 ...
-
bioRxiv - Biochemistry 2020Quote: ... Lysing Matrix Tubes with 0.5 g Lysing Matrix D (1.4 mm ceramic spheres) were purchased from MP Biomedicals (Seven Hills, NSW, Australia). PDA was purchased from Sigma-Aldrich.
-
bioRxiv - Microbiology 2020Quote: ... 293T cells were seeded at 65-75% confluency into 24-well plates coated for 30 min with a 1 mg/mL solution of poly-D-lysine (MP Biomedicals Inc.) and washed with PBS (Gibco ...
-
bioRxiv - Molecular Biology 2019Quote: ... with protease inhibitors (25 μg/mL aprotinin and 10 μg/mL leupeptin) in a FastPrep-24 homogenizer with specifically designed Lysing Matrix D tubes (MP Biomedicals, USA). The homogenates were centrifuged and the supernatants were aliquoted ...
-
bioRxiv - Genomics 2022Quote: Approximately 1mm thick tissue section with pellet was sectioned from SPF colons in OCT and put in dry ice-cold Lysis matrix D tube (MP Biomedicals, USA). 400μl of RLT buffer (Qiagen ...
-
bioRxiv - Cell Biology 2022Quote: ... that were either poly-D-lysine coated or had been treated with 50 μl of 0.2 mg/ml concanavalin A (MP Biomedicals, Solon, OH). Cells were imaged by confocal microscopy using a Nikon Eclipse Ti2-E A1R inverted microscope (Nikon ...
-
bioRxiv - Neuroscience 2023Quote: ... five biological replicates each containing five larva or flies per genotype were homogenised in 500 μL 10% methanol using Lysing Matrix D tubes (MP Biomedicals, OH). Fly homogenates or cell extracts (10 μg total protein ...
-
bioRxiv - Microbiology 2023Quote: ... and fetuses were weighed and homogenized in 1:10 weight per volume of PBS using Lysing Matrix D tubes (MP Biomedicals #6913100) in a MP Fast-prep 24 5G instrument ...
-
bioRxiv - Neuroscience 2021Quote: ... Frozen material was placed into a 2mL RNASE/DNASE free Lysing Matrix D tube filled with beads (MP Biomedicals Catalog No. 6913-500) for disruption ...
-
bioRxiv - Cancer Biology 2022Quote: ... The epidermis was dissociated from the dermis using a fresh scalpels with stainless steel surgical blades (Bard- Paker®, #371610) and transferred the epidermis to Lysing Matrix D (MP biomedicals, #6913100). For histone protein extraction ...
-
bioRxiv - Immunology 2020Quote: ... the collected cells were vortexed for 30 seconds and were transferred to FastPrep® lysing matrix D tubes (MP Biomedicals; Auckland, New Zealand). To achieve cell lysis ...
-
bioRxiv - Molecular Biology 2024Quote: ... tissue pieces after collection were snap frozen in 2 ml screw cap tubes containing Lysing Matrix D (Cat# 6540-434, Lot# 99999, MP Biomedicals, Irvine, USA). Second ...
-
bioRxiv - Neuroscience 2019Quote: ... Corticosterone double-antibody radioimmunoassay kits (MP biomedicals, Solon, OH) were used according to manufacturer’s instructions and the Inter- and intra-assay coefficients of variation were < 10%.
-
bioRxiv - Molecular Biology 2023Quote: ... using the following antibodies: anti-actin (MP Biomedicals, 691001), anti-NF-YA (Santa Cruz Biotechnology ...
-
bioRxiv - Physiology 2023Quote: ... Antibodies used included Actin (MP Biomedicals 691001, 1:100) and ALDH1A3 (Novus Biologicals NBP2-15339 ...
-
bioRxiv - Neuroscience 2020Quote: Samples of gray and white matter were collected from frozen frontal cortex sections using a 3mm biopsy punch and placed into individual 2mL FastPrep Lysing Matrix D RNA tubes (116913050 MP Biomedicals, Santa Ana, CA). 1 mL of RNA STAT-60 (Amsbio LLC ...
-
bioRxiv - Bioengineering 2023Quote: ... To assist the elution of biomolecules from nNs and glass chips ultrasonication or ceramic beads (MP Biomedicals, Lysing matrix D, 2 mL tube, 6913050) with 20 s ...
-
bioRxiv - Immunology 2023Quote: ... the colon tissue pieces were further minced before adding to a Lysing Matrix D tube that contains beads (Cat#: 6913050, MP Biomedicals, Santa Ana, CA). After quick mix by inverting the tube several times ...
-
bioRxiv - Genomics 2022Quote: ... All sections (∼11-16 sections per block) throughout 2-3 embedded worm pieces were collected in Lysing Matrix D tubes (MP Biomedicals, 2mL, Cat Ref. 6913100). The extraction was done with replicates of the originally embedded blocks and re-embedded blocks where the worm piece was re-embedded onto flattened OCT on a cryo chuck from the same batch as those used for Spatial Transcriptomics ...
-
bioRxiv - Neuroscience 2020Quote: ... to 1/1000 dilution or anti-Actin antibody (MP Biomedicals) to 1/5000 dilution or anti-TOM20(Cell Signaling ...
-
bioRxiv - Molecular Biology 2022Quote: ... Membranes were incubated overnight with SERF2 (1/1500, Protein tech) or Actin (1/10 000, MP biomedicals) antibody ...
-
bioRxiv - Developmental Biology 2021Quote: ... rabbit anti-β-galactosidase antibody 1:2000 (Cappel, 55976, MP Biomedicals), rabbit anti-Laminin A antibody at 1:200 (a gift from Herwig O ...
-
bioRxiv - Developmental Biology 2021Quote: ... We used anti-β-galactosidase antibody (MP Biomedicals (Irvine, CA, USA), #02150039 ...
-
bioRxiv - Neuroscience 2022Quote: ... and 1:30,000 mouse anti-actin antibody C4 (MP Biomedicals, catalog #08691001). Antibodies were diluted in 5% BSA in TBST + 0.05% sodium azide ...
-
bioRxiv - Molecular Biology 2020Quote: ... Anti-AGE monoclonal antibodies for western blotting were obtained from MP Biomedicals, USA ...
-
bioRxiv - Neuroscience 2020Quote: ... The following antibodies were used: rabbit anti-βgal (1:40K, MP Biomedicals) and rabbit anti-RFP (1:500 ...
-
bioRxiv - Neuroscience 2019Quote: ... CORT was measured using ImmuChem Double Antibody 125I Radioimmunoassay Kit (MP Biomedicals). The cross-reactivity with other steroids is less than 0.4% ...
-
bioRxiv - Genetics 2020Quote: ... or mouse monoclonal anti-actin antibody 1:5000 (MP Biomedicals, catalog 08691001), followed by incubation with goat anti-mouse horseradish peroxidase–conjugated secondary antibodies 1:10000 (Jackson Immuno Research ...
-
bioRxiv - Neuroscience 2020Quote: Antibodies used in Drosophila stainings were anti-HRP-FITC (MP Biomedicals, 0855977, RRID:AB_2334736), phalloidin-rhodamine (abcam ...
-
bioRxiv - Immunology 2022Quote: ... Immune complexes were stained with anti-guinea pig C3 fluorescent antibody (MP Biomedicals). Excess staining antibody was removed by washing and immune complexes were quantified on the iQue 3 VBR using Forecyt software (Intellicyt) ...
-
bioRxiv - Immunology 2020Quote: ... and stained with an anti-guinea pig C3 antibody conjugated to FITC (MP Biomedicals) for 15 min at ambient temperature ...
-
bioRxiv - Cell Biology 2019Quote: ... Horseradish peroxidase-conjugated goat anti-rabbit antibodies were from MP Biomedicals (Santa Ana, CA) or Jackson ImmunoResearch (West Grove ...
-
bioRxiv - Microbiology 2022Quote: ... or stained with a FITC-conjugated anti-mouse C3 antibody (MP Biomedicals, 1:200) for 1h at RT ...
-
bioRxiv - Cancer Biology 2023Quote: ... β-actin antibody was purchased from MP Biomedicals (Cat. No. 8691001, 1:20 000).
-
bioRxiv - Cell Biology 2023Quote: ... Horseradish peroxidase-conjugated goat anti-rabbit antibodies were from MP Biomedicals (Santa Ana, CA). Anti-phosphoserine/threonine antibodies were from ECM Biosciences (catalog # PM3801).
-
bioRxiv - Bioengineering 2024Quote: ... mice were intradermally injected with rabbit anti-BSA antibody (6 mg/ml, MP Biomedicals) in 25 μl of PBS ...
-
bioRxiv - Microbiology 2019Quote: For protein extraction, cells were lysed (in 1 % sodium deoxycholate, 50 mM triethylammonium bicarbonate) using the FastPrep-96 Instrument (MP Biomedicals) for 1 min at 1600 rpm and the All FastDNA-96 (MP Biomedicals ...
-
bioRxiv - Microbiology 2021Quote: ... and the insoluble inclusion body fraction was collected (fraction determined to contain recombinant proteins) and solubilized in 4 ml PBS containing 10% (w/v) sarkosyl (MP Biomedicals) with rocking overnight at 4°C ...
-
bioRxiv - Developmental Biology 2020Quote: ... and stained with the following primary antibodies: rabbit anti–β-galactosidase (1:10000; MP Biomedicals), mouse 4D9 anti-Engrailed (1:5 Developmental Studies Hybridoma Bank) ...
-
bioRxiv - Microbiology 2020Quote: ... followed by incubation with horseradish peroxidase (HRP) conjugated sheep anti-mouse secondary antibody (MP Biomedicals) 1:5000 diluted in blocking buffer for 30 min at room temperature ...
-
bioRxiv - Immunology 2020Quote: ... and stained with an FITC-conjugated anti-guinea pig C3 antibody (MP Biomedicals, Cat # 855385). C3 deposition onto beads was analyzed by flow cytometry ...
-
bioRxiv - Immunology 2024Quote: ... cells were incubated with goat anti-rabbit polyclonal antibody against C3-FITC conjugated (MP Biomedicals) for 1 h on ice ...
-
bioRxiv - Developmental Biology 2022Quote: ... Maternal serum samples collected at 0.6 G were analyzed for C-reactive protein (CRP) using an hsCRP ELISA kit (MP Biomedicals, Solon, OH) according to the manufacturer’s protocol with 1:100 serum dilution and were analyzed for IL-6 using an old world monkey IL-6 ELISA kit (U-CyTech Biosciences ...
-
bioRxiv - Immunology 2021Quote: ... The saliva sample containing 100 µg protein in Tris-HCl buffer (90 µL in total) was mixed with 5 µL of 100 mM DL-dithiothreitol (MP Biomedicals, LLC) for the reduction solution and heated at 56°C for 45 min ...
-
bioRxiv - Molecular Biology 2024Quote: ... and ectopic protein expression in Flp-In T-REx HeLa cells was induced with 1 μg/mL doxycycline (MP Biomedicals, 195044, Dox) minimum three hours post-seeding ...
-
bioRxiv - Physiology 2021Quote: For pulse labeling of newly translated proteins MIN6 cells were incubated in complete DMEM media but without cysteine and methionine (MP Biomedicals, #SKU 091646454) for 1hr ...
-
bioRxiv - Developmental Biology 2020Quote: ... Primary antibodies used were: rabbit and mouse anti-βGal (1/1000; Promega and MP Biomedicals, respectiveley), rat anti-Ser (1/1000 ...
-
bioRxiv - Cell Biology 2023Quote: ... for thiAp-arfA-GFP and an anti-actin monoclonal (C4) antibody (SKU0869100-CF, MP Biomedicals, Europe). Blots were developed using the Lumi Sensor Chemiluminescent HRP Substrate kit (Genscript ...
-
bioRxiv - Microbiology 2020Quote: ... and -actin (monoclonal antibody raised against actin from chicken gizzard, MP Biomedicals, Eschwege, Germany; 1:500 dilution). Secondary -mouse IgG-HRP conjugate (Promega ...
-
bioRxiv - Cell Biology 2019Quote: Commercial HRP-conjugated polyclonal goat anti-rabbit or goat anti-mouse secondary antibodies (MP Biomedicals, Solon, OH) were utilized at 2 μg/ml ...