Labshake search
Citations for MP Biomedicals :
351 - 400 of 408 citations for Semicarbazide Hydrochloride Sem 13C 99%; 15N2 98% 100 Ug Ml In Methanol since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2022Quote: Total RNA was prepared from 10 mL of 8 h culture following the procedure described for the FastRNA pro blue kit (MP Biomedicals) with the Fastprep apparatus (MP Biomedicals) ...
-
bioRxiv - Microbiology 2022Quote: ... bacteria were spotted onto BHI solidified with 1.5% agar (Oxoid Technical No. 3) supplemented with 500 µg/mL streptomycin (MP Biomedicals, USA) or 25 µg/mL rifampicin (Sigma-Aldrich ...
-
bioRxiv - Neuroscience 2022Quote: ... 293T cells were seeded at 40-60% confluency into 24-well plates coated for 30 min with a 0.1 mg/mL solution of poly-D-lysine (MP Biomedicals Inc.) and washed with PBS (Gibco ...
-
bioRxiv - Biophysics 2023Quote: ... All culture media were supplemented with 4 mg/ml low fatty acid bovine serum albumin (BSA, MP Biomedicals, AlbumiNZ, Auckland, NZ) unless specified otherwise ...
-
bioRxiv - Microbiology 2023Quote: CFU enumeration of bacterial burden was performed by collecting samples in 1 mL PBS followed by homogenization in a FastPrep-24 (MP Biomedicals) at 5.5 m/s for 2 minutes ...
-
bioRxiv - Biophysics 2023Quote: ... All culture media were supplemented with 4 mg/ml low fatty acid bovine serum albumin (BSA, MP Biomedicals, AlbumiNZ, Auckland, NZ) unless specified otherwise ...
-
bioRxiv - Bioengineering 2023Quote: ... The cell suspension was kept on ice until it was mixed with ice-cold 150 µL solutions of 6 mg/mL bovine fibrinogen (MP Biomedicals) to give a final concentration of 3 mg/mL fibrinogen per construct ...
-
bioRxiv - Cancer Biology 2023Quote: ... Stably transfected or lentivirally transduced cells were selected and maintained in the same medium supplemented with 0.5 to 1 µg/mL puromycin (MP Biomedicals 194539) or 4 µg/mL blasticidin (US Biological Life Sciences B2104-30) ...
-
bioRxiv - Neuroscience 2023Quote: ... the medium was changed to N2 medium supplemented with 1:2 of the day 1 supplements as well as 5 μg/ml puromycin (100552, MP biomedicals) to select for infected cells ...
-
bioRxiv - Microbiology 2023Quote: ... with 1 min of cooling on ice in between each cycle) in 2 ml bead beating tubes (Lysing Matrix E, MP Biomedicals) using a Bead Ruptor Elite 24 (Omni International) ...
-
Higher meiotic chromosome condensation: a potential function of kinetochore through polo-like kinasebioRxiv - Cell Biology 2023Quote: ... The cells were resuspended in 0.5 ml of spheroplasting solution supplemented with 5 µl of β-mercaptoethanol and 12.5 µl of zymolyase 20T (10 mg/ml, MP Biomedicals) and incubated at 30⁰C for 1.5 hrs ...
-
bioRxiv - Bioengineering 2023Quote: Stock sodium alginate solutions were prepared at a concentration of 30 mg/ml alginic acid sodium salt (low viscosity, MP Biomedicals) in PBS ...
-
bioRxiv - Microbiology 2024Quote: ... Bacterial burden was determined by collecting colon samples into 1 mL of reduced PBS and homogenized in a FastPrep-24 (MP Biomedicals) at 5.5 m/s for 2 minutes ...
-
bioRxiv - Microbiology 2024Quote: ... We removed the preservation solution and resuspended the fecal pellets in 1 mL of InhibitEX Buffer in Matrix E (MP Biomedicals) bead beating tubes ...
-
bioRxiv - Microbiology 2023Quote: ... Gut samples were homogenized in 1 ml of sterile PBS in bead-beating tubes containing zirconia beads using a FastPrep-25 5G apparatus (MP Biomedicals) set at 6 m s-1 for 30 sec ...
-
bioRxiv - Molecular Biology 2024Quote: ... which were then scraped off of the dishes and transferred to 2 ml microcentrifuge tube containing Lysing Matrix-D (MP Biomedicals). Cells were lysed by vortexing 6 times (20 seconds each ...
-
bioRxiv - Evolutionary Biology 2020Quote: ... Purified polyclonal antibody against NveAGO1 or NveAGO2 was added to 5 ml of TBST containing 5% BSA (1:1000) (MP Biomedicals, USA), to the membrane in a sealed sterile plastic bag and incubated at 4°C overnight ...
-
bioRxiv - Cancer Biology 2022Quote: ... Viral infections were carried out over 72 hours and transduced cells were selected by their resistance to 2 μg/mL puromycin (MP Biomedicals 0219453910).
-
bioRxiv - Evolutionary Biology 2022Quote: ... Tissue samples were placed into tubes containing 1 ml of TRIzol with a ceramic bead (1.4mm) and homogenized using a FastPrep-24 Instrument (MP Biomedicals, CA, USA). RNA extraction followed the protocol of TRIzol RNA extraction from Thermo Fischer Scientific ...
-
bioRxiv - Microbiology 2019Quote: Filter membranes were aseptically removed from the Sterivex cartridge and transferred to 2 ml Lysing Matrix E tubes (SKU 116914100, MP Biomedicals, USA) and DNA extraction and purification were performed in a Maxwell® 16 DNA extraction system (Promega ...
-
bioRxiv - Cancer Biology 2020Quote: ... Viral transfections were carried out over 72 hours ID8 parental cells and transduced cells were selected by their resistance to 2 μg/mL puromycin (MP Biomedicals 0219453910). Prior to use in this experiment ...
-
bioRxiv - Cell Biology 2019Quote: ... Cells were then resuspended in the buffer (1 M sorbitol, 25 mM KH2PO4, 50 mM β-mercaptoethanol) containing Zymolase −20T (20mg/ml - MP biomedicals). pH of the buffer was adjusted to 6.5 using NaOH ...
-
bioRxiv - Immunology 2021Quote: ... 2×107 cells of HAP1.MR1 clone D9 were seeded in a T175 flask on Day 0 and transduced with concentrated gene-trap retrovirus in the presence of 8 µg/ml of protamine sulphate (MP Biomedicals #P4380) for three consecutive rounds of transduction on Days 1-3 before expansion and freezing ...
-
bioRxiv - Microbiology 2020Quote: ... 293T cells were seeded at 65-75% confluency into 24-well plates coated for 30 min with a 1 mg/mL solution of poly-D-lysine (MP Biomedicals Inc.) and washed with PBS (Gibco ...
-
bioRxiv - Plant Biology 2022Quote: ... A total of 100 mg of mixed leaf tissue from each sample were placed in a 2 mL FastPrep® tube containing a 6.35 mm ceramic bead (MP BIOMEDICALS, USA) for homogenization and DNA extraction ...
-
bioRxiv - Plant Biology 2019Quote: ... with 1.5 mL KH2PO4 buffer and ground in a FastPrep at 6.0 m/s for 40 s (MP Biomedicals, Santa Ana, CA). Homogenized samples were poured into 15 mL centrifuge tubes and mixed with 1.5 mL sample diluent ...
-
bioRxiv - Physiology 2020Quote: ... Whole-body tissues from each sample were mechanically homogenized in 1 mL of Acetonitrile solution (50%, 50% ddH2O) using sterile glass beads and a homogenizer (FastPrep-24™ -MP Biomedicals) for 2 x 35 sec at 3800 rpm ...
-
bioRxiv - Cell Biology 2022Quote: ... cells were resuspended in 20 µL of [1 mg/mL] 100T zymolyase (cat. no. 08320932, MP Biomedicals or cat. no. 320932, VWR) and incubated at room temperature for 6-7 minutes ...
-
bioRxiv - Cancer Biology 2022Quote: ... LNCaP and 22Rv1 cells were transduced at a multiplicity of infection (MOI) of 0.5 and selected with 1 µg/ml puromycin (MP Biomedicals Cat #194539) for 7 days ...
-
bioRxiv - Microbiology 2022Quote: ... 10 μg ml-1 uracil and 0.73 g l-1 yeast methionine/uracil drop-out medium (CSM-Met-Ura, MP Biomedicals, USA, Solon) and lacked NZ-amine ...
-
bioRxiv - Genomics 2022Quote: ... a small fragment of Porites lobata was placed in 2 ml of Lysing Matrix A beads (MP Biomedicals, Santa Ana, CA, USA) in presence of 1.5 ml of DNA/RNA shield (Zymo Research ...
-
bioRxiv - Genomics 2022Quote: ... ~10 mg fresh urediniospores were placed in 2 mL screw-gap tubes filled with Lysing Matrix C and ground twice in MP FastPrep-24 TM 5G (Mp Biomedicals, USA) with a speed setting of 4 for 20 seconds ...
-
bioRxiv - Cell Biology 2022Quote: ... that were either poly-D-lysine coated or had been treated with 50 μl of 0.2 mg/ml concanavalin A (MP Biomedicals, Solon, OH). Cells were imaged by confocal microscopy using a Nikon Eclipse Ti2-E A1R inverted microscope (Nikon ...
-
bioRxiv - Biochemistry 2023Quote: ... Cells were resuspended in 5 ml/g cells Zymolyase buffer (5 mM β–Mercaptoethanol, 1 M Sorbitol) and 2 mg/g cells of Zymolyase (MP Biomedicals) was added followed by incubation at 30°C for 30 minutes with constant agitation ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... Mycelial tissue was scraped off from plates and homogenized in 2 ml Lysing Matrix E tubes (MP Biomedicals, Santa Ana, CA, USA) on a FastPrep-24 (MP Biomedicals ...
-
bioRxiv - Cell Biology 2024Quote: HEK293A or NG2-OSBP cells were seeded at approximately 50% confluence on 20 µg/ml entactin-collagen-laminin coated 8-well glass slides (MP biomedicals ICN6040805) and returned to the incubator ...
-
bioRxiv - Systems Biology 2024Quote: ... The vial was then added with 200 μL of 12 M acetic acid and 5 mL Ecolume scintillation liquid (MP Biomedicals, 0188247001) and vigorously vortexed ...
-
bioRxiv - Molecular Biology 2024Quote: ... and ectopic protein expression in Flp-In T-REx HeLa cells was induced with 1 μg/mL doxycycline (MP Biomedicals, 195044, Dox) minimum three hours post-seeding ...
-
bioRxiv - Microbiology 2021Quote: ... After heating the samples for 5 min at 95 °C we transferred the liquid to a 2 mL Lysis Matrix E tube (MP Biomedicals, Lucerne, Switzerland) and bead beated 2-times for 40 sec at 6 m/sec using a FastPrep™ 24 5G Bead Beating System (MP Biomedicals) ...
-
bioRxiv - Molecular Biology 2020Quote: ... 0 or 125 U/mL benzonase) with glass beads three times for 30 s in a Fastprep instrument (MP Biomedicals, Santa Ana, CA). The lysate was incubated 1 h at 4°C ...
-
bioRxiv - Immunology 2020Quote: ... ICs for M2b polarization were generated by mixing 1 µL of a 1.32 mg/mL solution of bovine serum albumin (BSA; MP Biomedicals; Auckland, New Zealand) with 50 µL anti-albumin (bovine serum) ...
-
bioRxiv - Immunology 2021Quote: ... 2-3 cm pieces of ilea were homogenized in 1 ml TriFast™ twice for 18 sec with 6 m/s using a FastPrep®-12 homogenizer (MP Biomedicals). Homogenates were centrifuged with 12,000 g for 10 min at 4 °C ...
-
bioRxiv - Immunology 2021Quote: ... Two-three cm pieces of ilea were homogenized in 1 ml TriFast™ twice for 18 sec with 6 m/s using a FastPrep®-12 homogenizer (MP Biomedicals). Homogenates were centrifuged at 12,000 g for 5 min and supernatant were diluted 1:10 in TriFast™ ...
-
bioRxiv - Genomics 2021Quote: ... the bead constituents (i.e., ceramic and silica spheres, and glass bed) contained within the 2 ml Lysing Matrix E tubes (MP Biomedicals, Cat. No.: 116914100) were aseptically transferred into sterile 1.5 ml microcentrifuge tubes (Eppendorf ...
-
bioRxiv - Genomics 2022Quote: ... These tubes were then incubated at 65°C overnight (12-18 hours) in 1 ml of PW1 solution and then homogenized using a FastPrep 24TM 5G Bead Beater (MP BioMedicals, Irvine, CA) using the following settings ...
-
bioRxiv - Biochemistry 2022Quote: ... the cell lysates were scraped off the wells and collected into scintillation vials containing 7 mL EcoLite Liquid Scintillation Cocktail (MP Biomedicals: 01882475-CF). [14C] radioactivity in counts-per-minute (CPM ...
-
bioRxiv - Microbiology 2024Quote: ... insects were placed in 2 mL sterile tubes containing 500 µL of PBS and 1.4 mm ceramic lysing beads (MP Biomedicals, Solon, OH, USA) and crushed using a Fast Prep instrument (MP Biomedicals ...
-
bioRxiv - Microbiology 2024Quote: ... Mice were sacrified by cervical dislocation after 24 h and the left lung was placed in 2 mL sterile tubes containing 600 μL of PBS and 1.4 mm ceramic lysing beads (MP Biomedicals, Solon, OH, USA). Organs were homogenized using a FastPrep instrument (MP Biomedicals ...
-
bioRxiv - Molecular Biology 2024Quote: ... tissue pieces after collection were snap frozen in 2 ml screw cap tubes containing Lysing Matrix D (Cat# 6540-434, Lot# 99999, MP Biomedicals, Irvine, USA). Second ...
-
bioRxiv - Microbiology 2024Quote: ... Pellets were disrupted with 0.1 mm ZrO2 beads (CarlRoth, GmbH) in 15 mL plastic tubes using a bead beater (MP Biomedicals, FastPrep-24 5G). A total of 6 cycles of beating were applied (3 times of application of the pre-installed program for M ...