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Citations for MP Biomedicals :
1 - 50 of 85 citations for PCR Buffers since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2020Quote: ... 1x PCR Direct Loading Buffer (MP Biomedicals, CA), 0.4 μM of each pair of primers (Martín-Hernández et al. ...
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bioRxiv - Animal Behavior and Cognition 2021Quote: ... PCRs were performed with the Taq Polymerase Direct Loading Buffer (5 U/μL; MP Biomedicals, CA) following manufacturer’s instructions ...
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bioRxiv - Bioengineering 2022Quote: ... and PCR products on 1% agarose gel made with Tris-Acetate-EDTA buffer diluted to 1X (TAW50X01, MP Biomedicals) at 100V for 45 min.
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bioRxiv - Microbiology 2022Quote: 45μl PCR product was purified with Gene Clean Turbo for PCR kit (MP Biomedicals) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... 122 μL MT buffer (MP Biomedicals) in 978 μL was added to 200 mg of fecal pellets in a sterile microcentrifuge tube ...
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bioRxiv - Neuroscience 2024Quote: ... in 1X TAE buffer (MP Biomedicals). TrackIt 100 bp DNA Ladder (Thermo Fisher ...
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bioRxiv - Microbiology 2019Quote: ... in a 50 μl PCR reaction with lx Taq-&GO Ready-to-use PCR Mix (MP Biomedicals, Germany) (98°C – 4 min ...
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bioRxiv - Microbiology 2021Quote: ... Cell lines were routinely assessed for Mycoplasma contamination by PCR using a Myco-Sniff Mycoplasma PCR Detection Kit (MP Biomedicals). All parasite lines used and generated in this study are listed in Table S1.
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bioRxiv - Genetics 2023Quote: ... 1.2 M sorbitol) and resuspended in digestion buffer (Buffer B, 200 mM Vanadyl ribonucleoside complex [VRC from NEB], zymolyse [zymolase 100T, MP Biomedicals]). Cells were digested at 30°C for 20 min and then gently washed with 1 mL of cold Buffer B and resuspended in 1 mL of 70% ethanol for 3.5-5 h.
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bioRxiv - Immunology 2022Quote: ... Live cells were purified using lymphocyte isolation buffer (MP Biomedicals), followed by stimulation with plate bound anti-CD3 (2 μg/ml ...
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bioRxiv - Microbiology 2020Quote: ... PCR were performed either with Taq Core DNA polymerase (MP Biomedicals), or with Phusion Hot Start DNA polymerase (Finnzymes ...
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bioRxiv - Biochemistry 2019Quote: ... Ub-tauK18 or tauK18 were diluted in PBS Buffer (MP Biomedicals) containing 0.01% sodium azide to 10 µM final concentration and incubated at 37°C ...
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bioRxiv - Neuroscience 2022Quote: Brains were dissected in Phosphate Buffer Solution (PBS; MP Biomedicals LLC). Adult optic lobes (OL ...
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bioRxiv - Immunology 2019Quote: ... PCRs were performed on cDNA using the Taq Core Kit (MP Biomedicals) and appropriate primer pairs (primer pairs are provided in Supplementary table 1) ...
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bioRxiv - Microbiology 2019Quote: ... PCR products were cleaned using a GENECLEAN (MP Biomedicals, Santa Ana, CA) and resuspended in sterile water.
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bioRxiv - Biochemistry 2020Quote: ... per 50 mL of buffer) and disrupted using FastPrep homogeniser (MP Biomedicals) with 0.1 mm Zirconium beads (Techtum ...
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bioRxiv - Systems Biology 2022Quote: ... using a BSA calibration curve (MP BIomedicals #160069, diluted in lysis buffer) on a Bio-Rad SmartSpec 3000 spectrophotometer instrument ...
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bioRxiv - Microbiology 2022Quote: ... per 50 mL of buffer) and disrupted using FastPrep homogeniser (MP Biomedicals) with 0.1 mm Zirconium beads (Techtum ...
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bioRxiv - Molecular Biology 2023Quote: ... using a BSA calibration curve (MP BIomedicals #160069, diluted in lysis buffer) on a Bio-Rad SmartSpec 3000 spectrophotometer instrument ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... bead-beat in buffer RLT using Lysing Matrix E tubes (MP Biomedicals), and extracted using AllPrep DNA/RNA/Protein Mini kit (Qiagen) ...
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bioRxiv - Biochemistry 2024Quote: ... using a BSA calibration curve (MP Biomedicals #160069, diluted in lysis buffer) on a Bio-Rad SmartSpec 3000 spectrophotometer instrument ...
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bioRxiv - Cell Biology 2022Quote: The hMIGA2 sample was buffer exchanged to 200 mM ammonium acetate (MP Biomedicals), 2 mM DTT with Zeba Spin Desalting Columns (Thermo-Fisher Scientific) ...
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bioRxiv - Microbiology 2021Quote: ... Triplicate PCR products were pooled and purified using the Geneclean Turbo kit (MP Biomedicals). Partial adapters were added by ligation at the sequencing facility (Genewiz ...
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bioRxiv - Microbiology 2019Quote: Spleens were homogenised in Cell Lytic buffer using Lysing matrix E beads (MP Biomedicals) and homogenates cleared by centrifugation at 10,000 rpm for 15 minutes ...
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bioRxiv - Microbiology 2019Quote: ... the buffer was replaced with 0.007% Triton X-100 (MP Biomedicals, Santa Ana, CA) containing 1 mM ATP and 0.01 mM ADP or 0 mM ATP or 1 mM ADP or 1 mM ATP and 0.5 mM Na3VO4 ...
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bioRxiv - Microbiology 2020Quote: ... cells were broken up in lysis buffer 73 using a FastPrep instrument (MP Biomedicals; settings ...
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bioRxiv - Biochemistry 2023Quote: ... except the cells were inoculated in Terrific Broth II buffer (TBII powder, MP Biomedicals) supplemented with kanamycin ...
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bioRxiv - Cancer Biology 2019Quote: ... cells were washed with 1X PBS buffer and stained with 0.5% crystal violet (MP Biomedicals) for 20 minutes ...
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bioRxiv - Microbiology 2021Quote: ... and blocked for 60 min in blocking buffer (10% filtered normal goat serum (MP Biomedicals) in PBS ...
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bioRxiv - Cell Biology 2021Quote: ... The cells were then incubated in blocking buffer 1% Bovine Serum Albumin (MP Biomedicals, #160069) and 5% goat-serum (Gibco #6210-064 ...
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bioRxiv - Cancer Biology 2019Quote: ... Frozen tibiae were homogenized in RIPA buffer (50) using a FastPrep-24 tissue homogenizer (MP Biomedicals). Prostate cancer cell lines were lysed using RIPA buffer as described previously (50) ...
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bioRxiv - Genomics 2022Quote: ... cordatum were resuspended in solubilization buffer and disrupted by bead beating (FastPrep-24 5G, MP Biomedicals) for 10 s at 6 m/s followed by 90 s on ice (in three repetitions ...
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bioRxiv - Cell Biology 2022Quote: ... cell pellets were lysed in the kit’s assay buffer using lysing matrix Y tubes (MP Biomedicals) containing 0.5 mm diameter yttria-stabilized zirconium oxide beads and a cell disruptor ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were resuspended in chilled extraction buffer and lysed using lysing matrix Y tubes (MP Biomedicals) containing 0.5 mm diameter yttria-stabilized zirconium oxide beads and a cell disruptor ...
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bioRxiv - Plant Biology 2020Quote: Cells were lysed in RIPA buffer containing protease inhibitors with glass beads using FastPrep (MP Biomedicals, CA) for 20s ...
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bioRxiv - Cell Biology 2020Quote: Cells were lysed by agitation in SDS sample buffer with glass beads using FastPrep (MP Biomedicals, OH) for 20 seconds ...
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bioRxiv - Microbiology 2020Quote: ... 300 µL of lysis buffer was added to lysing matrix E tubes (MP Biomedicals, Cat. No. 116914500). Samples were homogenized using the FastPrep-24 instrument at 6 m/s for 40s prior to centrifugation at maximum speed for 5 min ...
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bioRxiv - Molecular Biology 2022Quote: ... Cells were pelleted and resuspended in 0.5 ml digestion buffer + 0.5 mg/ml 100T Zymolase (MP biomedicals), and then incubated on a shaker (30°C ...
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bioRxiv - Cell Biology 2023Quote: ... Cell pellets were then resuspended in NAD+/NADH extraction buffer with lysing matrix Y tubes (MP Biomedicals), containing 0.5 mm diameter zirconium oxide beads ...
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bioRxiv - Developmental Biology 2021Quote: ... Blocking was carried out in wash buffer supplemented with 1% bovine serum albumin (BSA; MP Biomedicals; Cat. #160069). Primary antibodies were diluted and incubated overnight at 4°C in wash buffer supplemented with 0.3 % BSA ...
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bioRxiv - Plant Biology 2021Quote: ... The tissue was ground with Dellaporta isolation buffer in the Fast Prep®-24 Instrument (MP Biomedicals, USA) and extracted with chloroform before DNA precipitation ...
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bioRxiv - Cancer Biology 2020Quote: GBM cell lines were washed twice with cold 1X PBS before being collected in RIPA buffer (50 mM Tris pH 7.4, 150 mM NaCl, 0.1% SDS, 0.5% sodium deoxycholate(#102906, MP Biomedicals), 1% Nonidet™ P-40 substitute (#74385 ...
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bioRxiv - Microbiology 2023Quote: ... Cells were resuspended in Lysis buffer and were disrupted with silica beads by bead-beating machine (MP Biomedicals™ FastPrep-24™ 5G Instrument ...
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bioRxiv - Pathology 2021Quote: ... The PCR product was assessed by 1% agarose gel electrophoresis and cleaned using GeneClean Turbo Kit (MP biomedicals, USA) silica gel columns.
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bioRxiv - Microbiology 2020Quote: ... using the lysis buffer SL1 and a bead-beating mechanical lysis step performed on a FastPrep-24 (MP Biomedicals) tissue homogenizer at 6 m/s for 30 sec ...
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bioRxiv - Cell Biology 2023Quote: ... 250 μL supernatant was transferred to a new sterile tube which containing equal volume of PPS buffer (MP Biomedicals), and centrifuged at 14,000 ×Lg for 5 min ...
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bioRxiv - Microbiology 2019Quote: ... 125 mM pH 7.4 Tris buffer) and transferred to a Lysing Matrix E tube (MP Biomedicals, Santa Ana CA, USA), with 500 µl 25:24:1 phenol/chloroform/isoamyl alcohol ...
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bioRxiv - Molecular Biology 2020Quote: ... 5 μM B-ME) then spheroplasted using Zymolyase in wash buffer (10mg/ml final concentration, 20.000 U/g, MP Biomedicals) and left at 30°C 4 min ...
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bioRxiv - Microbiology 2021Quote: ... The cell lysis was performed in the lysis buffer of the kit with 50 mg glass beads (0.1 mm silica spheres, MP Biomedicals) in a bead beater (5G-FastPrep ...
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bioRxiv - Molecular Biology 2020Quote: ... Spheroblasts were resuspended in lysis buffer (50mM Tris-HCl, pH 8.0, 50mM EDTA, 100mM NaCl, 1.5% sarkosyl) and digested with proteinase K (MP Biomedicals 219350480) at 42° for 2-2.5h ...