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Citations for MP Biomedicals :
1 - 50 of 62 citations for Crimean Congo Haemorrhagic Fever virus CCHFV glycoprotein C Gc sheep Fc tag since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... Congo red plates were KB agar plates containing 1.0% agar (MP Biomedicals, UK) and rifampicin at 25 μg/ml ...
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bioRxiv - Cell Biology 2023Quote: ... Sheep Red blood cells (10% suspension) and rabbit anti-Sheep antibodies (IgG fraction) were purchased from MP biomedicals.
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bioRxiv - Microbiology 2022Quote: Sheep red blood cells (sRBCs) (MP Biomedicals) were opsonized with iC3b in gelatin veronal buffer ...
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bioRxiv - Microbiology 2021Quote: ... Infection proceeded for 1 hour at 37°C followed by overlay with virus growth medium containing 0.1% (w/v) immunodiffusion agarose (MP Biomedicals) and incubation was continued for 96 hours ...
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bioRxiv - Cell Biology 2021Quote: ... Sheep red blood cells (SRBC; 10% suspension) were from MP Biomedicals. Anti-sheep red blood cell antibodies were from Cedarlane Laboratories ...
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bioRxiv - Microbiology 2019Quote: ... 2 % FCS (MP Biomedicals, Santa Ana, CA), 100 U/mL penicillin ...
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bioRxiv - Microbiology 2020Quote: ... followed by incubation with horseradish peroxidase (HRP) conjugated sheep anti-mouse secondary antibody (MP Biomedicals) 1:5000 diluted in blocking buffer for 30 min at room temperature ...
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bioRxiv - Microbiology 2020Quote: ... 2 ml/well of virus growth medium containing 0.1% (w/v) immunodiffusion agarose (MP Biomedicals) was added and incubation was continued for 96 hours ...
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bioRxiv - Microbiology 2020Quote: ... the membrane was incubated in 1:5000 dilution of the peroxidase-conjugated sheep anti-mouse IgG secondary antibody (MP Biomedicals) for 30 min at room temperature ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Fc receptors were blocked for 30 min with Protein A (MP Biomedicals, OH) in Stain Buffer (554656 ...
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bioRxiv - Immunology 2022Quote: ... resuspended in 200 μl of the virus-containing supernatant supplemented with 10 μg/ml protamine sulfate (MP Biomedicals), and plated in u-bottom 96-well plates ...
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bioRxiv - Immunology 2023Quote: ... propagation of the virus was carried out in Vero (African green monkey kidney epithelial – ATCC: CCL-81) cells cultured in MEM (MP Biomedicals), supplemented with 1 µg/mL TPCK-Trypsin (Trypsin-Worthington) ...
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bioRxiv - Biochemistry 2022Quote: ... Affinity-purification using TeV-ProA tag to Rpn11 was conducted by incubating the cleared lysates with 30 μL IgG resin (MP Biomedicals, 855961) for 2 hrs at 4°C ...
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bioRxiv - Genetics 2020Quote: ... Cells were homogenized with Matrix C (MP Biomedicals) using a bead beater (MP Biomedicals ...
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bioRxiv - Genetics 2020Quote: ... Cell lysis was performed in Lysematrix C tubes (MP Biomedicals) using the FastPrep (MP Biomedicals ...
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bioRxiv - Genetics 2020Quote: ... and equal volume of glass beads (lysing matrix C, MP Biomedicals). Cell lysates were generated by homogenizing cells with a FastPrep-24 5G homogenizer (MP Biomedicals ...
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bioRxiv - Cell Biology 2023Quote: ... for 4 hr at 4°C over a 30% sucrose (MP Biomedicals) cushion ...
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bioRxiv - Microbiology 2021Quote: Cells were harvested at 4 °C and lysed using a Fastprep (MP biomedicals) in 50 mM Tris (pH 8.0) ...
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A GID E3 ligase assembly ubiquitinates an Rsp5 E3 adaptor and regulates plasma membrane transportersbioRxiv - Cell Biology 2021Quote: ... and transferred to a 2 mL tube containing lysing matrix C (MP Biomedicals). Cells were lysed by 3 rounds of 20 seconds in a Fast-Prep24 instrument (MP Biomedicals) ...
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bioRxiv - Cell Biology 2022Quote: ... chilled to -20°C and transferred to lysing matrix Y tubes (MP Biomedicals) containing 0.5 mm diameter yttria-stabilized zirconium oxide beads ...
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bioRxiv - Microbiology 2023Quote: ... Bacteria were grown at 37 °C in tryptic soy broth (TSB) (MP Biomedicals) and shaking at 200 rotations per minute ...
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bioRxiv - Cell Biology 2022Quote: Cells were grown overnight at 30°C in complete synthetic medium (CSM, MP Biomedicals) with 2% dextrose to mid-log phase (106-107 cells/mL) ...
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bioRxiv - Cell Biology 2022Quote: ... Worms were lysed with lysing matrix C tubes (#6912-100, MP Biomedicals, CA, USA) and the TissueLyser II (Qiagen ...
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bioRxiv - Molecular Biology 2022Quote: ... protease inhibitor cocktail) and lysed in lysing Matrix C tubes by FastPrep (MP Biomedicals; 6.5 M/s ...
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bioRxiv - Microbiology 2019Quote: ... The chlamydospore suspension was transferred to a Matric C grinding tube (MP Biomedicals, Santa Ana, California, USA) followed by RNA/DNA extraction as it was done for mycelia from pure cultures.
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... and incubated for 15 minutes at 37 °C in a 12% Proteinase K solution (MP Biomedicals, 0219350490). The enzymatic digestion was stopped by the addition of 20% FBS (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2021Quote: ... slices were treated for 30 minutes at 60°C in 1% ethanolic phosphotungstic acid (PTA; MP Biomedicals). Slices were washed with pure ethanol and subsequently with pure acetone ...
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bioRxiv - Molecular Biology 2022Quote: ... The cell suspension was then transferred to a 2ml FastPrep™ tube (lysing matrix C, MP Biomedicals) and lysis was carried out by 6 x 1min maximum speed cycles on a FastPrep-24™ cell homogeniser (MP Biomedicals) ...
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bioRxiv - Bioengineering 2023Quote: ... Bead beating was carried out at 4 °C on a FastPrep-24 5G machine from MP Biomedicals using their 2mL tubes pre-filled with Lysing Matrix Y ...
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bioRxiv - Developmental Biology 2019Quote: ... Plasma was collected and stored at −20 °C until radioimmunoassay (MP Biomedicals, The Netherlands; sensitivity 3 ng/ml).
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bioRxiv - Neuroscience 2019Quote: ... Plasma was kept at −20°C until processed with a I-125 radioimmunoassay (RIA) kit from MP Biomedicals. Duplicates were run for each sample for technical replication.
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bioRxiv - Physiology 2022Quote: ... Bacteria were stored at -80°C in 25% glycerol in autoclaved Luria-Bertani (LB) broth media (MP Biomedicals) and propagated on LB-agar plates containing chloramphenicol (10 ug/mL) ...
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bioRxiv - Microbiology 2022Quote: ... aliquot stocks were made and frozen at −80°C with 16% sterile glycerol (MP Biomedicals, cat. no. 76019-966). To enumerate CFU/mL in stocks ...
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bioRxiv - Microbiology 2022Quote: ... aliquot stocks were made and frozen at −80°C with 16% sterile glycerol (MP Biomedicals, cat. no. 76019-966). To enumerate colony forming units (CFU)/mL in stocks ...
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bioRxiv - Microbiology 2023Quote: ... the sample was added to a 2 mL tube containing lysis matrix C (MP Biomedicals, Santa Ana, CA, USA) and homogenized using a Vortex mixer ...
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bioRxiv - Immunology 2020Quote: ... ATCC 51459) were culture aerobically at 37°C overnight at 200 rpm in Luria-Bertani (LB) broth medium (MP Biomedicals) and then diluted 1:10 in fresh LB medium until the concentration of bacteria reached optical density 600 ...
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bioRxiv - Cell Biology 2019Quote: ... They were digested for 3 h at 37°C in a moist chamber with a digestion mix (0.3% (w/v) pectolyase Y-23 (MP Biomedicals), 0.3% (w/v ...
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bioRxiv - Cell Biology 2022Quote: ... Cell lysis was carried out at 4°C using a FastPrep-24™ Cell Disruptor (Cat. No. 6004500, MP Biomedicals) set at 6.5 for 8 cycles of 1 min each ...
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bioRxiv - Microbiology 2022Quote: ... water bathed for 30 min at 60 °C before bead beating (speed 6.0 for 45 seconds) using a FastPrep-24 instrument (MP Biomedicals). A blank extraction (without sediment addition ...
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bioRxiv - Genetics 2023Quote: ... buds were digested for 20 min at 37 °C after fixation in 0.3% (w/v) Pectolyase Y-23 (MP Biomedicals), 0.3% (w/v ...
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bioRxiv - Plant Biology 2024Quote: ... Samples were digested for 1 hour 45 min at 37°C (digestionmix: 0.3% (w/v) Pectolyase Y-23 (MP Biomedicals), 0.3% (w/v ...
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bioRxiv - Microbiology 2020Quote: ... homogenised in Lysing Matrix F tubes using a FastPrep 24G instrument (MP Biomedicals, 4°C, speed 6, 40 s, 2 cycles), and centrifuged (10,000 x g ...
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bioRxiv - Synthetic Biology 2021Quote: ... The cell pellets were resuspended into BMM-C at an OD600 of approximately 1 and then inoculated into BMM-xylose (5 g L-1 xylose, MP Biomedicals) to an OD600 of 0.025 in a 96-well plate (Greiner Bio-One) ...
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bioRxiv - Microbiology 2022Quote: ... cerevisiae S288C was used as the heterologous expression host to probe the roles of host proteins in the function of OspB and was routinely cultured at 30 °C in yeast extract-peptone-dextrose (YPD) broth or in synthetic selective media (MP Biomedicals) lacking histidine ...
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bioRxiv - Microbiology 2022Quote: ... was cultivated for 24-48 h at 28 °C on tryptic soy agar (TSA, Oxoid), in tryptone soy broth (TSB, Oxoid) or M9 minimal medium (MP Biomedicals) supplemented with glucose (Sigma-Aldrich ...
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bioRxiv - Microbiology 2022Quote: ... followed by centrifugation at 5000 rpm for 10 minutes at 4°C and finally RNA extraction using the FastRNA Pro Blue Kit (MP Biomedicals) following the instruction of the supplier ...
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bioRxiv - Microbiology 2023Quote: ... solani was cultivated for 24-48 h at 28 °C on tryptone soya agar (TSA, Oxoid), in tryptone soya broth (TSB, Oxoid) or M9 medium (MP Biomedicals) supplemented with 0.4 % glucose (final concentration 0.4%) ...
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bioRxiv - Microbiology 2023Quote: ... 10 mg/mL lysozyme treatment at 37°C for 10 min and 2x 30 sec bead beat in Lysing Matrix B tubes (MP Biomedicals) at setting 6 on a FastPrep24 (MP Bio ...
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bioRxiv - Developmental Biology 2022Quote: ... Maternal serum samples collected at 0.6 G were analyzed for C-reactive protein (CRP) using an hsCRP ELISA kit (MP Biomedicals, Solon, OH) according to the manufacturer’s protocol with 1:100 serum dilution and were analyzed for IL-6 using an old world monkey IL-6 ELISA kit (U-CyTech Biosciences ...
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bioRxiv - Biochemistry 2021Quote: ... and water in a ratio 1:0.85 (w/v) at 4°C and homogenized in Fastprep®-24 Instrument (MP Biomedicals, USA). Subsequently ...