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Citations for MP Biomedicals :
401 - 441 of 441 citations for B Cell Activating Factor BAFF TNFSF13B Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... Transduction was carried out two times for 24 hours and transduced cells were selected with appropriate antibiotics (5 μg/ml blasticidin, MP Biomedicals, Santa Ana ...
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bioRxiv - Molecular Biology 2022Quote: ... were added to the pellet and bead-beaten at 6m/s for 30 seconds using an MP Fast Prep-24 Tissue and Cell Homogenizer (MP Biomedicals).
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bioRxiv - Microbiology 2022Quote: ... Harvested cells suspended in 100 μl of lysis buffer (74) were mechanically disrupted in FastPrep Lysis Matrix A tubes (MP Biomedicals) at 30 Hz for 10 min ...
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bioRxiv - Molecular Biology 2022Quote: ... and lysis was carried out by 6 x 1min maximum speed cycles on a FastPrep-24™ cell homogeniser (MP Biomedicals), with the samples being returned to ice for 5min between each cycle ...
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bioRxiv - Microbiology 2022Quote: ... the larvae/embryos were mixed with 100 μL PBS in 1.5 mL tubes and homogenized with 3 cycles of 15 s at 6,500 rpm with rest intervals of 60 s by FastPrepTM 24-5G tissue-cell homogenizer (MP Biomedicals, USA). The homogenates were clarified by centrifugation at 9,000 × g for 5 min and the supernatants were used as the virus suspension for RT-qPCR analysis or further investigation (passaging of viruses by being injected to new zebrafish embryos ...
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bioRxiv - Cell Biology 2023Quote: ... 10 μM ZnSO4 and 2 mM PMSF and cells were lysed by glass bead lysis in the FastPrep-24 (MP Biomedicals). Pho8 activity was monitored by incubation of 200 μl lysate with 50 μl buffer 250 mM Tris–HCl ...
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bioRxiv - Microbiology 2023Quote: ... Cells were disrupted by bead beating for 40 seconds at 6.5m/s in a FastPrep-24 instrument (MP Biomedicals, United states). The supernatant was transferred to an Eppendorf tube and chloroform (Sigma-Aldrich ...
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bioRxiv - Immunology 2023Quote: ... propagation of the virus was carried out in Vero (African green monkey kidney epithelial – ATCC: CCL-81) cells cultured in MEM (MP Biomedicals), supplemented with 1 µg/mL TPCK-Trypsin (Trypsin-Worthington) ...
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bioRxiv - Cell Biology 2023Quote: ... 500 µl acid-washed glass beads were added and cells were mechanically lysed twice in a FastPrep device (6m/sec for 40 seconds; MP Biomedicals), with a 5-minute incubation on ice in between ...
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bioRxiv - Microbiology 2023Quote: ... One mL of the culture was centrifuged at 10,000 × g for 10 minutes and the cell pellet was used for DNA extraction using the FastDNA SPIN Kit (MP Biomedicals).
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bioRxiv - Cell Biology 2023Quote: ... 300 µl acid-washed glass beads were added and cells were mechanically lysed twice with a FastPrep device (6m/sec for 40 seconds; MP Biomedicals) with a 5-minute incubation on ice in between ...
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bioRxiv - Cancer Biology 2023Quote: ... Stably transfected or lentivirally transduced cells were selected and maintained in the same medium supplemented with 0.5 to 1 µg/mL puromycin (MP Biomedicals 194539) or 4 µg/mL blasticidin (US Biological Life Sciences B2104-30) ...
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bioRxiv - Cell Biology 2023Quote: ... 200 µl of acid-washed beads were added and cells were mechanically lysed twice in a FastPrep device (6m/sec for 40 seconds; MP Biomedicals), with a 5-minute incubation on ice in between ...
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bioRxiv - Microbiology 2023Quote: ... Only the initial cell lysis step was carried out in a FastPrep-24 instrument in lysing matrix A tubes (both MP Biomedicals) under different settings for the supernatant and cellular fraction samples ...
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bioRxiv - Immunology 2023Quote: ... DMEM (for labeling HeLa cells) or RPMI minus leucine (RPMI without L-leucine, L-glutamine, and sodium pyruvate from MP Biomedicals, supplemented with Glutamax and 1mM sodium pyruvate ...
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bioRxiv - Immunology 2024Quote: To further label viral RNA, cells were washed twice in Hybridization Wash Buffer (HWB) (2X SSC, 10% formamide (MP Biomedicals FORMD002), 0.2 mg/mL Bovine Seric Albumin (BSA ...
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bioRxiv - Molecular Biology 2024Quote: ... cells were distributed to two screw-cap microtubes containing 300 µl glass beads and lysed in a FastPrep instrument (MP Biomedicals) at 3 x 45 s 6 m/s ...
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bioRxiv - Cancer Biology 2022Quote: ... Viral infections were carried out over 72 hours and transduced cells were selected by their resistance to 2 μg/mL puromycin (MP Biomedicals 0219453910).
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bioRxiv - Neuroscience 2021Quote: ... 50,000/well freshly dispersed anterior pituitary cells were plated on poly-L-lysine-coated 8-well multitest slides (MP Biomedicals, Aurora, OH), bathed in Earle’s medium 199 supplemented with 2.2 g/L sodium bicarbonate ...
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bioRxiv - Cell Biology 2021Quote: ... for SA14-14-2 endogenous pools of cysteine and methionine were depleted by treating cells for 2 h with Cys− Met− MEM (MP Biomedicals #1641454) containing 7.5 μg/ml Actinomycin-D (AMD ...
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bioRxiv - Microbiology 2020Quote: The zebrafish larvae collected in Lysing Matrix Y tubes were homogenized with 3 cycles of 15 s at 6,500 RPM with rest intervals of 60 s by FastPrep™ 24-5G tissue-cell homogenizer (MP Biomedicals, USA). The homogenates were clarified by centrifugation at 9,000 × g for 5 min and RNA was extracted using RNeasy Mini Kit (Qiagen ...
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bioRxiv - Microbiology 2020Quote: The cells were fixed in 4% paraformaldehyde (Wako) and then permeabilized using 0.3% Triton X-100 (MP Biomedicals; Santa Ana, CA, USA). After blocking the cells by incubation in PBS containing 1% bovine serum albumin (BSA ...
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bioRxiv - Cell Biology 2022Quote: ... cells were resuspended in 20 µL of [1 mg/mL] 100T zymolyase (cat. no. 08320932, MP Biomedicals or cat. no. 320932, VWR) and incubated at room temperature for 6-7 minutes ...
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bioRxiv - Cancer Biology 2022Quote: ... LNCaP and 22Rv1 cells were transduced at a multiplicity of infection (MOI) of 0.5 and selected with 1 µg/ml puromycin (MP Biomedicals Cat #194539) for 7 days ...
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bioRxiv - Cancer Biology 2022Quote: ... This negative selection approach results in isolation of untouched CD8 or CD4 T cells and stored frozen with 10% DMSO (MP Biomedicals, 196055). In total ...
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bioRxiv - Cell Biology 2024Quote: HEK293A or NG2-OSBP cells were seeded at approximately 50% confluence on 20 µg/ml entactin-collagen-laminin coated 8-well glass slides (MP biomedicals ICN6040805) and returned to the incubator ...
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bioRxiv - Molecular Biology 2024Quote: ... and ectopic protein expression in Flp-In T-REx HeLa cells was induced with 1 μg/mL doxycycline (MP Biomedicals, 195044, Dox) minimum three hours post-seeding ...
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bioRxiv - Physiology 2021Quote: For pulse labeling of newly translated proteins MIN6 cells were incubated in complete DMEM media but without cysteine and methionine (MP Biomedicals, #SKU 091646454) for 1hr ...
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bioRxiv - Microbiology 2021Quote: ... viral-induced CPE and cytotoxicity of the compound were determined by measuring the viable cells using the methylthiazolyldiphenyl-tetrazolium bromide (MTT) assay (MP Biomedicals, NSW, Australia). Absorbance was measured at 540-650 nm on a plate reader.
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bioRxiv - Microbiology 2021Quote: ... Approximately 400 mg of 0.1 mm zircon/silica beads were added to the tube and subjected to mechanical cell lysis using a FastPrep-24 Classic bead beater (MP Biomedicals, Irvine, CA) at 4.0 m/s for 24 s ...
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bioRxiv - Immunology 2020Quote: ... the collected cells were vortexed for 30 seconds and were transferred to FastPrep® lysing matrix D tubes (MP Biomedicals; Auckland, New Zealand). To achieve cell lysis ...
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bioRxiv - Biochemistry 2022Quote: ... the cell lysates were scraped off the wells and collected into scintillation vials containing 7 mL EcoLite Liquid Scintillation Cocktail (MP Biomedicals: 01882475-CF). [14C] radioactivity in counts-per-minute (CPM ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were grown to semi-confluence in 35-mm Petri dishes containing HEK cell media [Dulbecco’s modified Eagle’s medium containing 10% fetal bovine serum (MP Biomedicals, Solon, OH, United States), 2.0 mM L-glutamine ...
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bioRxiv - Microbiology 2020Quote: ... were added and the cells were lysed for 5 min at 2000 rpm in a FastPrep VR-96 instrument (MP Biomedicals, Santa Ana, CA). The beads were then precipitated by centrifugation for 5 min at 30 130 g and the supernatant was collected ...
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bioRxiv - Cancer Biology 2019Quote: ... Treated cells were fixed in formalin (10% buffered formalin phosphate, Fischer Scientific, Hampton NH) and permeabilized with Triton-X100 (MP Biomedicals, St. Ana CA). A primary conjugated antibody against CD44 (BioLegend ...
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bioRxiv - Immunology 2020Quote: ... Ice cold 6% (v/v) PCA was added to the cells and the mixture was homogenized using a FASTPREP-24 (MP Biomedicals, Solon, Ohio, USA) and centrifuged at 4 °C ...
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bioRxiv - Bioengineering 2020Quote: ... Three 5 mm glass balls and 300 μL 10 mM MgCl2 were added to each tube and the plant cells were lysed at a speed of 4.0 m/s for 20 seconds in a FastPrep-24™ Instrument (MP Biomedicals, Illkirch-Graffenstaden, France) to release the live bacteria from the leaves ...
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bioRxiv - Immunology 2021Quote: ... Isolated PBMCs were then cryopreserved in a cryovial in cell recovery freezing medium containing 10% Dimethyl Sulfoxide (DMSO) (MP Biomedicals, LLC, Irvine, CA, USA) and 90% heat inactivated fetal bovine serum (FBS ...
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bioRxiv - Pathology 2024Quote: ... Some of the cells incubated in the 10%-FBS-DMEM culture medium, cells were further treated with thrombin (0, 0.5, 1, 2, or 4 U/mL) (MP Biomedicals Inc., Irvine, CA, USA) or hemin (0 ...
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bioRxiv - Microbiology 2020Quote: ... were added to the colon digesta slurry and cells were disrupted in 3 × 60 second cycles using a FastPrep24 (MP Biomedicals, Santa Ana, CA, USA). Debris were removed by centrifugation at 16.600 x g for 20 minutes and proteins were precipitated overnight in 16% ice-cold TCA ...
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bioRxiv - Biochemistry 2020Quote: ... 100 μL glass beads (ø=0.1-0.11 mm) was added and the cells were then disrupted at 4 °C by a FastPrep®-24 instrument (MP Biomedicals, 5 m/s, 20 sec, 3x with 5 min break). To remove the cell debris a centrifugation step was performed (25.000 × g ...