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Citations for MP Biomedicals :
251 - 300 of 304 citations for Galectin 1 LGALS1 Human His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: Strains were grown overnight and 1 ml of culture was transferred to a 2 ml Lysing matrix B tube (MP Biomedicals) and bead-beaten for 1 minute at 5 m/s twice ...
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bioRxiv - Molecular Biology 2023Quote: Metabolites were extracted via addition of 500 μL acetonitrile:methanol:water (2:2:1, v/v) and homogenized on dry ice with a bead beater (FastPrep-24; MP Biomedicals, CA, USA) at 6.0 m/s (3 x 30 s ...
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bioRxiv - Cancer Biology 2023Quote: ... Stably transfected or lentivirally transduced cells were selected and maintained in the same medium supplemented with 0.5 to 1 µg/mL puromycin (MP Biomedicals 194539) or 4 µg/mL blasticidin (US Biological Life Sciences B2104-30) ...
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bioRxiv - Microbiology 2023Quote: ... with 1 min of cooling on ice in between each cycle) in 2 ml bead beating tubes (Lysing Matrix E, MP Biomedicals) using a Bead Ruptor Elite 24 (Omni International) ...
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bioRxiv - Microbiology 2023Quote: ... Gut samples were homogenized in 1 ml of sterile PBS in bead-beating tubes containing zirconia beads using a FastPrep-25 5G apparatus (MP Biomedicals) set at 6 m s-1 for 30 sec ...
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bioRxiv - Microbiology 2024Quote: ... We removed the preservation solution and resuspended the fecal pellets in 1 mL of InhibitEX Buffer in Matrix E (MP Biomedicals) bead beating tubes ...
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bioRxiv - Microbiology 2024Quote: ... Bacterial burden was determined by collecting colon samples into 1 mL of reduced PBS and homogenized in a FastPrep-24 (MP Biomedicals) at 5.5 m/s for 2 minutes ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Purified polyclonal antibody against NveAGO1 or NveAGO2 was added to 5 ml of TBST containing 5% BSA (1:1000) (MP Biomedicals, USA), to the membrane in a sealed sterile plastic bag and incubated at 4°C overnight ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Tissue samples were placed into tubes containing 1 ml of TRIzol with a ceramic bead (1.4mm) and homogenized using a FastPrep-24 Instrument (MP Biomedicals, CA, USA). RNA extraction followed the protocol of TRIzol RNA extraction from Thermo Fischer Scientific ...
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bioRxiv - Systems Biology 2019Quote: ... at the ratio of 4:1) of 80% methanol (MS-grade) and lysed on a FastPrep-24 Instrument (MP Biomedicals, Burlingame, CA), using the lysing matrix D and suggested settings (speed 6 and 4 × 30 s pulses ...
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bioRxiv - Cell Biology 2019Quote: ... Cells were then resuspended in the buffer (1 M sorbitol, 25 mM KH2PO4, 50 mM β-mercaptoethanol) containing Zymolase −20T (20mg/ml - MP biomedicals). pH of the buffer was adjusted to 6.5 using NaOH ...
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bioRxiv - Neuroscience 2020Quote: ... were randomly assigned to either LA1 (Leukadherin-1; S8306, Selleckchem, TX) or) or vehicle (6% DMSO (Dimethyl Sulfoxide, ACS; 191418, MP Biomedicals, PA) in sterile saline with 1% Tween-20 (BP337500 ...
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bioRxiv - Cell Biology 2021Quote: ... we used the following primary and secondary antibodies at the indicated dilutions: rabbit anti-β-Gal (1:500, MP Biomedicals, cat. #55976), Rabbit anti-GFP (1:500 Invitrogen #A6455) ...
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bioRxiv - Microbiology 2020Quote: ... 293T cells were seeded at 65-75% confluency into 24-well plates coated for 30 min with a 1 mg/mL solution of poly-D-lysine (MP Biomedicals Inc.) and washed with PBS (Gibco ...
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bioRxiv - Biochemistry 2019Quote: ... which were exactly accord with the structure described (SI Appendix). DSS (white powder; CAS NO. 9011-18-1; LOT: Q6182; M.W. = 36000 – 50000 Da) was purchased from MP Biomedicals (Shanghai, China). Mesalazine (#A600043-0050 ...
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bioRxiv - Microbiology 2020Quote: ... The protocols used for soil DNA extraction were as follows: 1) Fast DNA™ SPIN kit for soil (MP Biomedicals, California, USA) by the manufacturer’s protocol ...
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bioRxiv - Physiology 2021Quote: ... Metabolic fluxes were assessed through the use of distinct radiolabeled tracers: 1) [U-14C]-glucose (0.12 mCi/L from MP Biomedicals; glucose oxidation); and 2 ...
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bioRxiv - Physiology 2020Quote: ... Whole-body tissues from each sample were mechanically homogenized in 1 mL of Acetonitrile solution (50%, 50% ddH2O) using sterile glass beads and a homogenizer (FastPrep-24™ -MP Biomedicals) for 2 x 35 sec at 3800 rpm ...
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bioRxiv - Cancer Biology 2021Quote: ... Low phosphate media was a 9:1 ratio of (RPMI-1640 without L-Glutamine and Phosphate +10% FBS; MP Biomedicals cat#: 09162975) with normal growth media (RPMI-1640 +10% Fetal Bovine Serum) ...
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bioRxiv - Biochemistry 2021Quote: ... and water in a ratio 1:0.85 (w/v) at 4°C and homogenized in Fastprep®-24 Instrument (MP Biomedicals, USA). Subsequently ...
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bioRxiv - Cell Biology 2022Quote: ... cells were resuspended in 20 µL of [1 mg/mL] 100T zymolyase (cat. no. 08320932, MP Biomedicals or cat. no. 320932, VWR) and incubated at room temperature for 6-7 minutes ...
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bioRxiv - Cancer Biology 2022Quote: ... LNCaP and 22Rv1 cells were transduced at a multiplicity of infection (MOI) of 0.5 and selected with 1 µg/ml puromycin (MP Biomedicals Cat #194539) for 7 days ...
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bioRxiv - Microbiology 2022Quote: DNA was extracted from environmental samples (1 mL) and mesocosm samples (pellet from 0.5 mL) using the FastDNA Spin Kit for Soil (MP Biomedicals, Irvine, CA) following the manufacturer’s guidelines ...
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bioRxiv - Microbiology 2023Quote: ... which was immediately emulsified at 4 ms−1 for 45 s using the FastPrep-24 bead beating system (MP Biomedicals, United States). The water-in-oil emulsion was aliquoted into 8 portions ...
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bioRxiv - Plant Biology 2023Quote: ... spiked with stable isotope labeled internal standards (1 pmol/sample) and were homogenized with 1.5 mM zirconium beads using a FastPrep-24 homogenizer (MP Biomedicals, CA, USA), and incubated at 4°C for 30 min ...
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bioRxiv - Bioengineering 2023Quote: Synthetic dextrose containing 6.7 g·L-1 of yeast nitrogen base without amino acids (DifcoLaboratories, Sparks, MD, USA), 0.77 g·L-1 of complete supplement mixture (CSM, without uracil and histidine) (MP Biomedicals, Solon, OH, USA), and 2% glucose were used on strains carrying HIS3 and URA3 marker(s) ...
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bioRxiv - Genomics 2023Quote: ... 30 mg of tissue were resuspended in a lysis buffer containing 1% β-mercaptoethanol and immediately homogenised using ceramic beads (MP Biomedicals) on a Bead Ruptor homogeniser (Omni Inc) ...
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bioRxiv - Microbiology 2023Quote: ... and fetuses were weighed and homogenized in 1:10 weight per volume of PBS using Lysing Matrix D tubes (MP Biomedicals #6913100) in a MP Fast-prep 24 5G instrument ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were resuspended in 5 ml/g cells Zymolyase buffer (5 mM β–Mercaptoethanol, 1 M Sorbitol) and 2 mg/g cells of Zymolyase (MP Biomedicals) was added followed by incubation at 30°C for 30 minutes with constant agitation ...
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bioRxiv - Immunology 2023Quote: ... Lipids were resuspended in 1 ml of 5 µg/ml butylated hydroxytoluene in 6:1 isopropanol:methanol.(Isopropanol, Fisher ChemicalTM; A461-4; Methanol, Fisher ChemicalTM; A456-4; butylated hydroxytoluene, MP Biomedicals™, 0210116280).
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bioRxiv - Molecular Biology 2024Quote: ... and ectopic protein expression in Flp-In T-REx HeLa cells was induced with 1 μg/mL doxycycline (MP Biomedicals, 195044, Dox) minimum three hours post-seeding ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... the female C57BL6/J mice were exposed to 0% (control; receiving water only) or 1% DSS (colitis; mol wt 36000–50000; MP Biomedicals, Eschwege, Germany) in drinking water for 5 consecutive days to induce intestinal inflammation ...
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bioRxiv - Molecular Biology 2020Quote: ... Plaque assays were performed as previously described [17] except that plates were either fixed for two days (1% gum tragacanth overlay-MP BIOMEDICALS catalog 0210479280) or three days (1.5% methylcellulose overlay-Spectrum Chemical catalog ME136-100GM ...
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bioRxiv - Immunology 2020Quote: ... ICs for M2b polarization were generated by mixing 1 µL of a 1.32 mg/mL solution of bovine serum albumin (BSA; MP Biomedicals; Auckland, New Zealand) with 50 µL anti-albumin (bovine serum) ...
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bioRxiv - Immunology 2021Quote: ... 2-3 cm pieces of ilea were homogenized in 1 ml TriFast™ twice for 18 sec with 6 m/s using a FastPrep®-12 homogenizer (MP Biomedicals). Homogenates were centrifuged with 12,000 g for 10 min at 4 °C ...
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bioRxiv - Immunology 2021Quote: ... Two-three cm pieces of ilea were homogenized in 1 ml TriFast™ twice for 18 sec with 6 m/s using a FastPrep®-12 homogenizer (MP Biomedicals). Homogenates were centrifuged at 12,000 g for 5 min and supernatant were diluted 1:10 in TriFast™ ...
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bioRxiv - Cell Biology 2022Quote: The following chemicals and compounds were used with doses enumerated in the figures and figure legends: Choline chloride (MP Biomedicals #67-48-1), Phosphocholine chloride calcium salt tetrahydrate (Sigma Aldrich #P0378) ...
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bioRxiv - Genomics 2022Quote: ... These tubes were then incubated at 65°C overnight (12-18 hours) in 1 ml of PW1 solution and then homogenized using a FastPrep 24TM 5G Bead Beater (MP BioMedicals, Irvine, CA) using the following settings ...
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bioRxiv - Genomics 2022Quote: Approximately 35 mg of frozen tissues were homogenized by bead beating for 20 s at a speed of 4.5 ms-1 on a FastPrep24 sample disruption system with Lysing Matrix S tubes (MP Biomedicals, Santa Ana, CA). Total RNA was isolated with the RNeasy Plus Mini Kit (Qiagen ...
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bioRxiv - Microbiology 2023Quote: ... cells were subjected first to hyperosmotic shock with 1 M sorbitol in LB and then treated with 5% N-lauroylsarcosine sodium salt (MP biomedicals, Cat. #190289) to remove the OM ...
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bioRxiv - Developmental Biology 2022Quote: ... the gel was blotted on methanol-activated PVDF membrane and anti-β-actin (1:5000; 08-691002; MP Biomedicals, Santa Ana, CA, USA) was used to detect the G- and the F-actin fractions ...
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bioRxiv - Immunology 2023Quote: ... portion of lung tissues in a screw cap vial containing 1 ml of DMEM media and 0.3 ml (wt/vol) of 1mm Zirconia/silica beads (MP Biomedicals, LLC, CA, USA). Tissues were lysed by using a FastPrep homogenizer (MP Biomedicals ...
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bioRxiv - Microbiology 2023Quote: ... washed three times in PBS with 0.05% tween 20 and incubated with a commercial anti-SARS-CoV-2 Spike antibody (1/1000) (MP Biomedicals, Irvine, CA, USA) or the in-house anti-SARS-CoV-2 mAb (1/400) ...
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bioRxiv - Microbiology 2020Quote: ... following manufacturer’s instructions and bead-beating samples for 45 s at 6 m s−1 on a FastPrep 5G homogenizer (MP Biomedicals, Santa Ana, CA). Extracted DNA was amplified in triplicate PCR ...
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bioRxiv - Microbiology 2020Quote: ... Aerobic growth assays conducted in M9 minimal media containing 1% NaCl (M9) supplemented with 20mM glucose (M9-Glucose) or M9 containing 2% (w/v) casamino acids (M9CAS) (MP Biomedicals, LLC. Solon, OH) supplemented with 20mM glucose (M9CAS-Glucose ...
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bioRxiv - Microbiology 2022Quote: ... 50 μl of 0.1 M ammonium aluminum sulfate and 0.5 mL of phenol:chloroform:isoamyl alcohol (25:24:1) were bead-beaten at 5.5 m/s for 45 s in a FastPrep instrument (MP Biomedicals, Solon, OH, United States). Following bead-beating ...
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bioRxiv - Microbiology 2020Quote: ... pH 8.0] in 1:4 w/v ratio and transfer into a 2 ml screw-cap tube with FastPrep Lysing matrix A (MP Biomedicals, Irvine, California, USA). Cells were mechanically disrupted by bead-beating for 2 × 3 min at 30 Hz (TissueLyser II ...
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bioRxiv - Microbiology 2023Quote: ... were added to a 2 ml microcentrifuge tube and the mixture was homogenized at 4 m/s for 1 minute using the MP Bio Fastprep-24TM (MP Biomedicals, Santa Ana, CA). The homogenized aliquots were then centrifuged for 5 min at 5,250 x g and 200 µl of the supernatant was transferred to a 2 ml collection tube.This aliquot contains viral targets from the solid fraction of the wastewater sample ...
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bioRxiv - Microbiology 2023Quote: ... A small fraction of about 4×4 mm from each tissue was transferred to a 2 mL vial with a 1/4” ceramic sphere (MP Biomedicals, Solon OH, USA) and 300 μL MagNA Pure 96 DNA Tissue Lysis Buffer (Roche Diagnostics GmbH ...
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bioRxiv - Pathology 2024Quote: ... Some of the cells incubated in the 10%-FBS-DMEM culture medium, cells were further treated with thrombin (0, 0.5, 1, 2, or 4 U/mL) (MP Biomedicals Inc., Irvine, CA, USA) or hemin (0 ...