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Citations for MP Biomedicals :
51 - 100 of 105 citations for 6 Methyloctanoic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... The mixture was homogenized at 6 m/second for 45 seconds in a Fast-Prep24TM5G homogenizer (MP Biomedicals) and centrifuged for 2 minutes at 2000 rpm ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Guts were homogenized at 6 ms−1 for 45 s using a FastPrep-24 5G homogenizer (MP Biomedicals). The five gut homogenates were pooled by hive of origin and serial dilutions of these pools from 10−3 to 10−12 were plated onto BHIA ...
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bioRxiv - Synthetic Biology 2020Quote: ... Cultures for flow cytometry were diluted into SDC (0.67% w/v Difco yeast nitrogen base without amino acids; 0.2% complete supplement mixture (MP Biomedicals); and 2% w/v dextrose) ...
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bioRxiv - Genomics 2019Quote: ... containing 100 µl 10% SDS and 500 µl acid phenol and beads beaded using a FastPrep instrument (MP Biomedicals) for 45 seconds at 5.5 m/s ...
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bioRxiv - Microbiology 2023Quote: ... 1mL of sample was homogenised with 250mg of fine (0.25 - 0.5mm) acid-washed glass beads in a FastPrep-24 Classic (MP Biomedicals). 4 x 35s cycles of homogenisation at 6.5m s−1 were used ...
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bioRxiv - Microbiology 2019Quote: ... followed by bead beating for 40 s at 6 m/s using a FastPrep 24 instrument (MP Biomedicals, USA), and centrifugation at 14,000g for 10 min ...
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bioRxiv - Molecular Biology 2020Quote: ... Cell pellets were lysed with 1 mL of acid-treated glass beads in a bead beater (FastPrep-5; MP Biomedicals) at level 7.5 for 15 s ...
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bioRxiv - Molecular Biology 2022Quote: ... cultures were grown in low fluorescence media (1.7 g/L Yeast Nitrogen Base without ammonium sulfate, without folic acid, without riboflavin [MP Biomedicals # 114030512] ...
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bioRxiv - Microbiology 2020Quote: ... then adding 90 µL to each well such that the final concentration of the bile acid after the addition of cells (10 µL) ranged from 0.04 mM to 10 mM for CA (102897, MP Biomedicals), and 0.01 mM to 2.5 mM for DCA (D6750 ...
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bioRxiv - Genomics 2021Quote: ... Composition of the nitrogen-depleted media was as follows: 0.67% Yeast Nitrogen Base without amino acids and ammonium sulfate (MP Biomedicals), 2% D-glucose ...
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bioRxiv - Physiology 2022Quote: ... Hearts were washed trice with PBS for 20 minutes each followed by antigen retrieval by incubating whole hearts in retrieval buffer (4% SDS (CN30.3, Carl Roth GmbH & Co. KG) and 200 mM boric acid (191411, MP Biomedicals)) for 1h at room temperature followed by overnight incubation at 54°C ...
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bioRxiv - Microbiology 2023Quote: ... and 600 μL was added to each PowerBead tube with 6 μL of ß-mecaptoethanol (MP Biomedicals, Irvine, CA, USA). Each tube was vortexed and homogenized on a FastPrep 24 Bead Beating Instrument for 20 seconds at 4.5 M/s for four rounds ...
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bioRxiv - Biochemistry 2022Quote: ... samples were mixed with 1 mL 80% methanol and 0.5 mL of 200- to 300-μm acid-washed glass beads (MilliporeSigma) and homogenized using a FastPrep-24 bead beater (MP Biomedicals), with intermittent cooling in dry ice/ethanol bath ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... yeast were selected for on plates lacking leucine and grown in SCD media lacking leucine (5 g/L ammonium sulfate, 1.7 g/L yeast nitrogen base without amino acids (MP Biomedicals; 4510522), 1 NaOH pellet (VWR ...
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bioRxiv - Molecular Biology 2023Quote: ... Pelleted cells were dissolved in 20 % (v/v) trichloroacetic acid (TCA) and lysed with glass beads by vortexing for 40 seconds on a Fastprep-24 5G (MP Biomedicals). Glass beads were removed and protein was pelleted by centrifugation ...
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bioRxiv - Biophysics 2023Quote: ... All culture media were supplemented with 4 mg/ml low fatty acid bovine serum albumin (BSA, MP Biomedicals, AlbumiNZ, Auckland, NZ) unless specified otherwise ...
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bioRxiv - Biophysics 2023Quote: ... All culture media were supplemented with 4 mg/ml low fatty acid bovine serum albumin (BSA, MP Biomedicals, AlbumiNZ, Auckland, NZ) unless specified otherwise ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were lysed with 500 μl acid-washed glass beads in 500 μl ALFA pull-down buffer using the FastPrep (MP biomedicals). After a centrifugation step at 1,000 g and 4 °C for 10 min ...
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bioRxiv - Bioengineering 2023Quote: Stock sodium alginate solutions were prepared at a concentration of 30 mg/ml alginic acid sodium salt (low viscosity, MP Biomedicals) in PBS ...
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bioRxiv - Bioengineering 2020Quote: ... The cells were lysed with acid-washed glass beads (400–650μm) in the FastPrep homogeniser (3x, 4m/s for 20 s) (MP Biomedicals, CA, USA). After centrifugation at 15,000g for 5min ...
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bioRxiv - Genetics 2020Quote: ... Specific nitrogen source and nitrogen depletion media was prepared immediately before use from 2X S+Uri (3.4 g/L Yeast Nitrogen Base without Amino Acids or ammonium sulfate (MP Biomedicals, #4027-032), 200 µg/mL uridine) ...
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bioRxiv - Bioengineering 2023Quote: Synthetic dextrose containing 6.7 g·L-1 of yeast nitrogen base without amino acids (DifcoLaboratories, Sparks, MD, USA), 0.77 g·L-1 of complete supplement mixture (CSM, without uracil and histidine) (MP Biomedicals, Solon, OH, USA), and 2% glucose were used on strains carrying HIS3 and URA3 marker(s) ...
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bioRxiv - Systems Biology 2024Quote: ... The vial was then added with 200 μL of 12 M acetic acid and 5 mL Ecolume scintillation liquid (MP Biomedicals, 0188247001) and vigorously vortexed ...
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bioRxiv - Biophysics 2021Quote: ... 50 mg of fecal pellet was subjected to bead beating (4x40 s at 6 m/s with 5 min incubations in between) in a Lysing Matrix E tube (MP Biomedicals) using a FastPrep-24 instrument ...
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bioRxiv - Microbiology 2020Quote: ... homogenised in Lysing Matrix F tubes using a FastPrep 24G instrument (MP Biomedicals, 4°C, speed 6, 40 s, 2 cycles), and centrifuged (10,000 x g ...
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bioRxiv - Microbiology 2022Quote: ... for 40 seconds at 6 m/s for 2 cycles in a FastPrep 24 5G bead grinder and lysis system (MP Biomedicals) for plaque assay or RNA isolation ...
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bioRxiv - Immunology 2022Quote: ... were subsequently added followed by an additional lysis step with matrix B at 6 m/s for 45 s on a FastPrep (MP Biomedicals). Following this ...
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bioRxiv - Microbiology 2021Quote: ... containing silica glass beads and homogenized for 40 s at 6 m/s using a FastPrep-24 5G homogenizer (MP Biomedicals) in the presence of 15 ml of preheated (65°C ...
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bioRxiv - Genomics 2021Quote: ... and bead beat at setting 6 for 40 sec using the FastPrep-24™ Classic Instrument (MP Biomedicals, Cat. No.: 116004500). The resulting homogenized mixture was then subjected to centrifugation at 14 000 x g for 10 min at 4°C using the Eppendorf® Centrifuge 5424R (Cat ...
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bioRxiv - Molecular Biology 2022Quote: ... and lysis was carried out by 6 x 1min maximum speed cycles on a FastPrep-24™ cell homogeniser (MP Biomedicals), with the samples being returned to ice for 5min between each cycle ...
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bioRxiv - Physiology 2022Quote: ... The samples were extracted 3 times for 20 seconds with 6 m/sec with 30 seconds breaks in using a FastPrep-24 5G bead beating grinder (MP Biomedicals) supplied with a CoolPrep adapter.
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bioRxiv - Physiology 2023Quote: Approximately 20 mg of mouse cecal content was weighed extracted by bead beating (3 times of 20s 6 m/s with 30s breaks) with FastPrep-24 5G bead beating grinder (MP Biomedicals) supplied with a CoolPrep adapter ...
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Dual RNA-seq identifies proteins and pathways modulated during Clostridioides difficile colonisationbioRxiv - Microbiology 2023Quote: ... in a FastPrep-24 5G (6.5 m/s for 20 seconds with 180 seconds rest on ice for 6 cycles) (MP Biomedicals, USA). RNA was extracted using the RNeasy RNA isolation kit ...
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bioRxiv - Microbiology 2023Quote: ... Cell lysates were disrupted by two rounds of lysis at 6 m/s for 60s (MP FastPrep-24 5G Tissue Homogenizer; MP Biomedicals). In between runs ...
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bioRxiv - Bioengineering 2023Quote: ... The cell suspension was kept on ice until it was mixed with ice-cold 150 µL solutions of 6 mg/mL bovine fibrinogen (MP Biomedicals) to give a final concentration of 3 mg/mL fibrinogen per construct ...
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bioRxiv - Microbiology 2023Quote: ... and samples underwent two bead-beating steps of 4 seconds at 6 m/s on a FastPrep-24 5G bead-beater (MP Biomedicals). Gut samples with total weight over 220 mg were diluted to reach a concentration of 220 mg in 650 μl lysis buffer + 25 μl DDT ...
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bioRxiv - Microbiology 2024Quote: ... using FastPrep lysing matrix A tubes at 6 m/s for 3 x 20 s in a Fast-Prep-24 homogenizer (MP Biomedicals). Serial dilutions of the homogenates were plated on TSA and incubated at 37°C to determine viable CFUs ...
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bioRxiv - Microbiology 2019Quote: ... at 4 m/s (fermented milk) and 6 m/s (kefir grains) for five times 20 seconds using a FastPrep®-24 instrument (MP Biomedicals). 150 µl 20 % SDS was added and after 5 min incubation at room temperature the tubes were centrifuged at maximum speed for 2 min ...
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bioRxiv - Neuroscience 2020Quote: ... were randomly assigned to either LA1 (Leukadherin-1; S8306, Selleckchem, TX) or) or vehicle (6% DMSO (Dimethyl Sulfoxide, ACS; 191418, MP Biomedicals, PA) in sterile saline with 1% Tween-20 (BP337500 ...
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bioRxiv - Plant Biology 2021Quote: ... Mycelium was homogenized for 40 seconds at 6 m/sec in a FastPrep-24™ 5G bead beating grinder and lysis system (MP Biomedicals). Genomic DNA was extracted using a DNA extraction kit (Wizard Genomic DNA Purification Kit ...
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bioRxiv - Microbiology 2021Quote: ... and bead beated 2-times for 40 sec at 6 m/sec using a FastPrep™ 24 5G Bead Beating System (MP Biomedicals). After the bead beating step ...
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bioRxiv - Microbiology 2020Quote: ... Samples were subjected to two rounds of bead beating with a speed setting 6 for 30 s using a FastPrep®-24 bead beater (MP Biomedicals). In between the beat beating steps ...
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bioRxiv - Immunology 2021Quote: ... mice were sacrificed and colon samples with content were homogenized twice for 18 sec at 6 m/s using a FastPrep®-12 homogeniser (MP Biomedicals). The homogenate was cleared by centrifugation at 5,000 rpm for 15 min ...
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bioRxiv - Immunology 2023Quote: ... Lipids were resuspended in 1 ml of 5 µg/ml butylated hydroxytoluene in 6:1 isopropanol:methanol.(Isopropanol, Fisher ChemicalTM; A461-4; Methanol, Fisher ChemicalTM; A456-4; butylated hydroxytoluene, MP Biomedicals™, 0210116280).
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bioRxiv - Microbiology 2020Quote: ... Aerobic growth assays conducted in M9 minimal media containing 1% NaCl (M9) supplemented with 20mM glucose (M9-Glucose) or M9 containing 2% (w/v) casamino acids (M9CAS) (MP Biomedicals, LLC. Solon, OH) supplemented with 20mM glucose (M9CAS-Glucose ...
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bioRxiv - Biophysics 2023Quote: ... Two-cell embryos were released from the oviducts by gently flushing the oviduct using pre-warmed Research Wash medium (ART Lab Solutions, SA, Australia) supplemented with 4 mg/ml low fatty acid bovine serum albumin (BSA, MP Biomedicals, AlbumiNZ, Auckland, NZ) using a 29-gauge insulin syringe with needle (Terumo Australia Pty Ltd ...
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bioRxiv - Cell Biology 2019Quote: Worm Lysate from synchronous L3 worm cultures was prepared in Laemmli buffer with 6 M urea with glass beads in a FastPrep machine (MP Biomedicals, Irvine, CA) for 2x 30 sec ...
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bioRxiv - Immunology 2021Quote: ... 2-3 cm pieces of ilea were homogenized in 1 ml TriFast™ twice for 18 sec with 6 m/s using a FastPrep®-12 homogenizer (MP Biomedicals). Homogenates were centrifuged with 12,000 g for 10 min at 4 °C ...
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bioRxiv - Immunology 2021Quote: ... Two-three cm pieces of ilea were homogenized in 1 ml TriFast™ twice for 18 sec with 6 m/s using a FastPrep®-12 homogenizer (MP Biomedicals). Homogenates were centrifuged at 12,000 g for 5 min and supernatant were diluted 1:10 in TriFast™ ...
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bioRxiv - Genomics 2023Quote: ... bead beating was performed three times for 30 s at 6 m/s using one tube of lysing matrix for each fraction (Cat.#6914-800, MP Biomedicals, Ohio, USA) and an MP Bio Fast Prep®-24 homogenizer (MP Biomedicals ...