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Citations for MP Biomedicals :
601 - 650 of 663 citations for PI 3065 CAS 955977 50 1 98% since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... 70 μL substrate solution (250 mM Tris-HCl pH 9, 25 mM MgSO4, 1% Triton, 2.7 mM p-nitrophenyl phosphate (MP Biomedicals)) was then added to each well ...
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bioRxiv - Plant Biology 2024Quote: ... Samples were digested for 1 hour 45 min at 37°C (digestionmix: 0.3% (w/v) Pectolyase Y-23 (MP Biomedicals), 0.3% (w/v ...
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bioRxiv - Microbiology 2023Quote: ... Cells were lysed by bead beating (2 cycles, 40 s each, 6.0 m s-1) using a FastPrep homogenizer (MP Biomedicals) and 0.1-mm silica glass beads (MP Biomedicals) ...
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bioRxiv - Cell Biology 2024Quote: ... the lysate was made from 1∼2 ml packed worms with 3∼4 ml of 0.5-mm diameter glass beads using FastPrep-24 (MP Biomedicals) in lysis buffer [pH 7.4 ...
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bioRxiv - Molecular Biology 2024Quote: ... One mL of each TRIzol cell suspensions was transferred into 2 mL tubes with 0.5 mL zirconium beads (0.1 – 0.2 µm in diameter) and cells were disrupted by shaking employing a FastPrep-24 bead beating instrument (MP Biomedicals) 2 x 30 s at 6 m/s ...
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bioRxiv - Immunology 2024Quote: ... samples were blocked with 5% milk and incubated with primary antibody anti-mouse C3 (1:500, cat#55463, MP Biomedicals) or anti-mouse C4 (1:200 ...
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bioRxiv - Microbiology 2021Quote: ... Lung samples were taken from the upper left lung lobe and were homogenized in 1 ml DMEM with ceramic and glass beads (Lysing Matrix H 500, 2 ml tube, MP Biomedicals) in a mixer mill (Retsch Schwingmühle MM 400 ...
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bioRxiv - Microbiology 2020Quote: ... The pellet was resuspended in 1 ml acetonitrile:methanol:water (4:4:2; v/v/v) mixture in lysing matrix tubes (MP Biomedicals, Eschwege, Germany) with glass beads ...
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bioRxiv - Microbiology 2020Quote: ... after all cells were completely broken using with bead-beating (0.5mm mixed 0.1 mm diameter ceramic beads at 3:1) on the Fastprep®-24 Sample Preparation System (MP Biomedicals, USA), run for 3 cycles each at the rate of 6M/s for 1min ...
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bioRxiv - Microbiology 2020Quote: ... for 15 min at 30°C with shaking (100 rpm) and then collected and suspended in buffer A with 1 mM DTT plus 100 mg of Zymolyase 20T (MP Biomedicals) per 10 g of pelleted cells ...
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bioRxiv - Cancer Biology 2021Quote: Metabolites were extracted from cell pellets using 3 mL 2:1 methanol-water solvent and 0.5 ml of lysing beads (Lysing Matrix D from MP Biomedicals, LLC). The mixture was vortexed and freeze-thawed to extract the metabolites ...
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bioRxiv - Cancer Biology 2022Quote: ... animals were exposed to 1∼3 cycles of 2% dextran sulfate sodium salt (DSS, MW=36,000-50,000, MP Biomedicals, CAT# 216011050). For in vivo inhibition of VPS9D1-AS1 ...
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bioRxiv - Cancer Biology 2022Quote: ... 1x pre-lysis buffer was added to the epidermis in the Lysing Matrix D tube and program 1 of FastPrep-24 homogenizer (MP Biomedicals) was used for homogenization of the epidermis ...
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bioRxiv - Microbiology 2023Quote: CFU enumeration of bacterial burden was performed by collecting samples in 1 mL PBS followed by homogenization in a FastPrep-24 (MP Biomedicals) at 5.5 m/s for 2 minutes ...
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bioRxiv - Microbiology 2023Quote: Strains were grown overnight and 1 ml of culture was transferred to a 2 ml Lysing matrix B tube (MP Biomedicals) and bead-beaten for 1 minute at 5 m/s twice ...
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bioRxiv - Microbiology 2024Quote: ... Bacterial burden was determined by collecting colon samples into 1 mL of reduced PBS and homogenized in a FastPrep-24 (MP Biomedicals) at 5.5 m/s for 2 minutes ...
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bioRxiv - Microbiology 2023Quote: ... with 1 min of cooling on ice in between each cycle) in 2 ml bead beating tubes (Lysing Matrix E, MP Biomedicals) using a Bead Ruptor Elite 24 (Omni International) ...
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bioRxiv - Microbiology 2023Quote: ... Gut samples were homogenized in 1 ml of sterile PBS in bead-beating tubes containing zirconia beads using a FastPrep-25 5G apparatus (MP Biomedicals) set at 6 m s-1 for 30 sec ...
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bioRxiv - Biochemistry 2022Quote: ... An equal number of cells (1-2×108 cells) was harvested and lysed using glass beads and a FastPrep Bead Beating System (MP Biomedicals). For denaturing cell lysis ...
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bioRxiv - Cell Biology 2022Quote: ... 0.093% of the total choline was radioactive ([Methyl-3H]-Choline Chloride; Perkin Elmer #NET109001MC)(Choline Chloride; MP Biomedicals #67-48-1). For example ...
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bioRxiv - Genetics 2022Quote: ... About 1 mm of tissue from the tips of roots were incubated in 2% cellulase Onozuka R-10 (Research Products Internationall) and 1% pectolyase Y-23 (MP Biomedicals) 37°C for 20-50 minutes to digest cell walls ...
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bioRxiv - Plant Biology 2022Quote: ... and glass beads of 0.75 to 1 mm diameter (Carl Roth GmbH) were added to each sample and bead milling was performed for 3 × 1 min in a homogenizer (FastPrep24, MP Biomedicals). The combined extracts were centrifuged and stored on ice until measurement on the same day.
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bioRxiv - Cancer Biology 2023Quote: ... Stably transfected or lentivirally transduced cells were selected and maintained in the same medium supplemented with 0.5 to 1 µg/mL puromycin (MP Biomedicals 194539) or 4 µg/mL blasticidin (US Biological Life Sciences B2104-30) ...
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bioRxiv - Cell Biology 2024Quote: ... protease inhibitor cocktail FY (Serva) 1/100) and lysed twice in a FastPrep device (6 m/s for 40 s; MP Biomedicals), with a 5-min incubation on ice in between ...
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bioRxiv - Plant Biology 2024Quote: ... Non-transformed yeast cells were cultivated in Yeast Peptone Dextrose complete medium (yeast extract 1%, peptone 2% and glucose D 2% w/v, YPD Broth Powder, MP Biomedicals). Transformed strains were grown in Yeast Nitrogen Base (YNB 6.7 g L-1 ...
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bioRxiv - Genomics 2024Quote: ... with 3 cycles of 1 min at 6m/s with 5 min interval on ice on the FastPrep-24 (MP Biomedicals) homogenizer ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Purified polyclonal antibody against NveAGO1 or NveAGO2 was added to 5 ml of TBST containing 5% BSA (1:1000) (MP Biomedicals, USA), to the membrane in a sealed sterile plastic bag and incubated at 4°C overnight ...
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bioRxiv - Neuroscience 2020Quote: ... were randomly assigned to either LA1 (Leukadherin-1; S8306, Selleckchem, TX) or) or vehicle (6% DMSO (Dimethyl Sulfoxide, ACS; 191418, MP Biomedicals, PA) in sterile saline with 1% Tween-20 (BP337500 ...
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bioRxiv - Cell Biology 2021Quote: ... we used the following primary and secondary antibodies at the indicated dilutions: rabbit anti-β-Gal (1:500, MP Biomedicals, cat. #55976), Rabbit anti-GFP (1:500 Invitrogen #A6455) ...
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bioRxiv - Microbiology 2020Quote: ... 293T cells were seeded at 65-75% confluency into 24-well plates coated for 30 min with a 1 mg/mL solution of poly-D-lysine (MP Biomedicals Inc.) and washed with PBS (Gibco ...
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bioRxiv - Microbiology 2020Quote: ... The protocols used for soil DNA extraction were as follows: 1) Fast DNA™ SPIN kit for soil (MP Biomedicals, California, USA) by the manufacturer’s protocol ...
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bioRxiv - Physiology 2021Quote: ... Metabolic fluxes were assessed through the use of distinct radiolabeled tracers: 1) [U-14C]-glucose (0.12 mCi/L from MP Biomedicals; glucose oxidation); and 2 ...
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bioRxiv - Cancer Biology 2021Quote: ... Low phosphate media was a 9:1 ratio of (RPMI-1640 without L-Glutamine and Phosphate +10% FBS; MP Biomedicals cat#: 09162975) with normal growth media (RPMI-1640 +10% Fetal Bovine Serum) ...
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bioRxiv - Biochemistry 2021Quote: ... and water in a ratio 1:0.85 (w/v) at 4°C and homogenized in Fastprep®-24 Instrument (MP Biomedicals, USA). Subsequently ...
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bioRxiv - Microbiology 2023Quote: ... which was immediately emulsified at 4 ms−1 for 45 s using the FastPrep-24 bead beating system (MP Biomedicals, United States). The water-in-oil emulsion was aliquoted into 8 portions ...
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bioRxiv - Bioengineering 2023Quote: Synthetic dextrose containing 6.7 g·L-1 of yeast nitrogen base without amino acids (DifcoLaboratories, Sparks, MD, USA), 0.77 g·L-1 of complete supplement mixture (CSM, without uracil and histidine) (MP Biomedicals, Solon, OH, USA), and 2% glucose were used on strains carrying HIS3 and URA3 marker(s) ...
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bioRxiv - Molecular Biology 2024Quote: ... and ectopic protein expression in Flp-In T-REx HeLa cells was induced with 1 μg/mL doxycycline (MP Biomedicals, 195044, Dox) minimum three hours post-seeding ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were resuspended in 5 ml/g cells Zymolyase buffer (5 mM β–Mercaptoethanol, 1 M Sorbitol) and 2 mg/g cells of Zymolyase (MP Biomedicals) was added followed by incubation at 30°C for 30 minutes with constant agitation ...
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bioRxiv - Immunology 2023Quote: ... Lipids were resuspended in 1 ml of 5 µg/ml butylated hydroxytoluene in 6:1 isopropanol:methanol.(Isopropanol, Fisher ChemicalTM; A461-4; Methanol, Fisher ChemicalTM; A456-4; butylated hydroxytoluene, MP Biomedicals™, 0210116280).
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bioRxiv - Microbiology 2023Quote: ... and fetuses were weighed and homogenized in 1:10 weight per volume of PBS using Lysing Matrix D tubes (MP Biomedicals #6913100) in a MP Fast-prep 24 5G instrument ...
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bioRxiv - Cell Biology 2022Quote: ... cells were resuspended in 20 µL of [1 mg/mL] 100T zymolyase (cat. no. 08320932, MP Biomedicals or cat. no. 320932, VWR) and incubated at room temperature for 6-7 minutes ...
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bioRxiv - Cancer Biology 2022Quote: ... LNCaP and 22Rv1 cells were transduced at a multiplicity of infection (MOI) of 0.5 and selected with 1 µg/ml puromycin (MP Biomedicals Cat #194539) for 7 days ...
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bioRxiv - Genomics 2023Quote: ... 30 mg of tissue were resuspended in a lysis buffer containing 1% β-mercaptoethanol and immediately homogenised using ceramic beads (MP Biomedicals) on a Bead Ruptor homogeniser (Omni Inc) ...
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bioRxiv - Microbiology 2024Quote: ... with the addition of 3x 1 minute bead beating cycles at speed 8 (with a 1-minute rest between each cycle) using a FastPrep 24 bead beater (MP Biomedicals, USA). The extracted DNA was stored at −80°C until it was ready to be analysed.
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bioRxiv - Neuroscience 2024Quote: ... A confluent 75 mm flask of PC12 cells was diluted 1:3 and plated in a 10 cm dish containing a multi-test 8-well slide (MP Biomedicals # 20190128). 24 hours later cells were transfected with 5 μg pacAd5CMV-CRECFP and
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bioRxiv - Microbiology 2024Quote: ... in 19 ml 95% (v/v) ethanol (Avantor Performance Material Deventer, NLD)) and 1 ml beef extract solution [100 μg/ml] (MP Biomedicals, USA). In case of C ...
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bioRxiv - Cell Biology 2024Quote: ... and SSNA-1(R18E) were insoluble and hence purified as previously described supplementing lysis and elution buffer with 8 M urea (MP Biomedicals #210569505). The proteins were refolded by dialysis against lysis buffer ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... the female C57BL6/J mice were exposed to 0% (control; receiving water only) or 1% DSS (colitis; mol wt 36000–50000; MP Biomedicals, Eschwege, Germany) in drinking water for 5 consecutive days to induce intestinal inflammation ...
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bioRxiv - Molecular Biology 2020Quote: ... Plaque assays were performed as previously described [17] except that plates were either fixed for two days (1% gum tragacanth overlay-MP BIOMEDICALS catalog 0210479280) or three days (1.5% methylcellulose overlay-Spectrum Chemical catalog ME136-100GM ...
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bioRxiv - Immunology 2020Quote: ... ICs for M2b polarization were generated by mixing 1 µL of a 1.32 mg/mL solution of bovine serum albumin (BSA; MP Biomedicals; Auckland, New Zealand) with 50 µL anti-albumin (bovine serum) ...