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Citations for MP Biomedicals :
551 - 600 of 625 citations for CU 115 CAS 2471982 20 2 98% since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2021Quote: ... and selected using 50 mg/L spectinomycin and 50 mg/L gentamycin to maintain the pMP90 plasmid (MP Biomedicals Inc., Santa Ana, CA, US). A colony PCR was performed as stated above with the initial 94°C step for 10 min to confirm transformation.
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bioRxiv - Microbiology 2020Quote: ... Cells were transferred to screw cap tubes pre-filled with 0.75 grams of glass beads (425-600 μm) and lysed using a FastPrep-24 5G (MP Biomedicals, Santa Ana, CA, USA) with 8 bursts at 6 m/s and 10 seconds per burst ...
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bioRxiv - Immunology 2021Quote: ... Isolated PBMCs were then cryopreserved in a cryovial in cell recovery freezing medium containing 10% Dimethyl Sulfoxide (DMSO) (MP Biomedicals, LLC, Irvine, CA, USA) and 90% heat inactivated fetal bovine serum (FBS ...
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bioRxiv - Microbiology 2023Quote: ... were added to a 2 ml microcentrifuge tube and the mixture was homogenized at 4 m/s for 1 minute using the MP Bio Fastprep-24TM (MP Biomedicals, Santa Ana, CA). The homogenized aliquots were then centrifuged for 5 min at 5,250 x g and 200 µl of the supernatant was transferred to a 2 ml collection tube.This aliquot contains viral targets from the solid fraction of the wastewater sample ...
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bioRxiv - Genomics 2022Quote: ... was thawed and disrupted by the simultaneous multidirectional striking using a high-speed homogenizer FastPrep-24 5G Instrument (MP Biomedicals, Santa Ana, CA, USA) (following conditions ...
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bioRxiv - Microbiology 2024Quote: ... transfected cells were fixed in 4% PFA in PBS for 15 min and subsequently permeabilized with 0.1% Triton X-100 (MP Biomedicals, Santa Ana, CA, USA) in PBS for 15 min ...
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bioRxiv - Bioengineering 2024Quote: ... Endothelium-independent vasodilatation was finally probed by administering increasing concentrations between 10−9 and 10−4 M of the nitric oxide (NO) donor sodium nitroprusside (MP Biomedicals; Irvine, CA, USA) through the lumen of pressurized ...
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bioRxiv - Immunology 2023Quote: ... the colon tissue pieces were further minced before adding to a Lysing Matrix D tube that contains beads (Cat#: 6913050, MP Biomedicals, Santa Ana, CA). After quick mix by inverting the tube several times ...
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bioRxiv - Genetics 2023Quote: ... Genomic DNA was extracted directly from 0.1 g to 0.4 g of each of the 359 faecal samples (i.e., right after the sample collection) using the FastDNA SPIN Kit for Soil (MP Biomedicals, Santa Ana, CA, USA).
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bioRxiv - Microbiology 2023Quote: ... Approximately 400 mg of 0.1 mm zirconia-silica beads were then added to the samples and placed in a FastPrep-24 Classic bead beater (MP Biomedicals, Irvine, CA, USA) and mechanically lysed at 6.0 m/s for 40 s ...
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bioRxiv - Microbiology 2024Quote: ... and mechanical disrupted in three homogenization cycles (each 30 s, 6.5 m/s) using a FastPrep-24 5G (MP Biomedicals, LLC, Irvine, CA, USA). Cell debris and glass beads were removed by centrifugation (13.300 rpm ...
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bioRxiv - Evolutionary Biology 2024Quote: ... and positive clones were grown and selected in Luria broth media supplemented with 50 μg/ml of Spectinomycin (158993, MP Biomedicals, Santa Ana, CA). The NucleoSpin 96 Plasmid kit (740625.4 ...
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bioRxiv - Microbiology 2020Quote: ... homogenised in Lysing Matrix F tubes using a FastPrep 24G instrument (MP Biomedicals, 4°C, speed 6, 40 s, 2 cycles), and centrifuged (10,000 x g ...
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bioRxiv - Microbiology 2021Quote: ... Lung samples were taken from the upper left lung lobe and were homogenized in 1 ml DMEM with ceramic and glass beads (Lysing Matrix H 500, 2 ml tube, MP Biomedicals) in a mixer mill (Retsch Schwingmühle MM 400 ...
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bioRxiv - Microbiology 2020Quote: ... The pellet was resuspended in 1 ml acetonitrile:methanol:water (4:4:2; v/v/v) mixture in lysing matrix tubes (MP Biomedicals, Eschwege, Germany) with glass beads ...
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bioRxiv - Microbiology 2022Quote: ... for 40 seconds at 6 m/s for 2 cycles in a FastPrep 24 5G bead grinder and lysis system (MP Biomedicals) for plaque assay or RNA isolation ...
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bioRxiv - Neuroscience 2022Quote: Seven- to nine-week-old male mice were treated with drinking water containing 2% DSS (molecular weight: 36,000 - 50,000, MP Biomedicals, Illkirch, France) or regular drinking water as a control ...
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bioRxiv - Evolutionary Biology 2021Quote: ... 300 mg of plant material was collected into a 2 ml screw cap tube filled with 4-5 porcelain beads and ground with a FastPrep tissue disruptor (MP Biomedicals). For DNA extraction ...
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bioRxiv - Cancer Biology 2021Quote: Metabolites were extracted from cell pellets using 3 mL 2:1 methanol-water solvent and 0.5 ml of lysing beads (Lysing Matrix D from MP Biomedicals, LLC). The mixture was vortexed and freeze-thawed to extract the metabolites ...
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bioRxiv - Immunology 2022Quote: ... the wells were washed with “washing buffer” (PBS x1 and 0.05% Tween20) and were blocked for 2 h at RT with 200 µL of “blocking buffer” (PBS x1, 3% BSA (MP Biomedicals), 20 mM EDTA ...
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bioRxiv - Cancer Biology 2022Quote: ... animals were exposed to 1∼3 cycles of 2% dextran sulfate sodium salt (DSS, MW=36,000-50,000, MP Biomedicals, CAT# 216011050). For in vivo inhibition of VPS9D1-AS1 ...
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bioRxiv - Pathology 2020Quote: Ela-Cre AT-1fl/+ and Ela-Cre AT-1fl/fl mice at 2-3 months of age were treated with tamoxifen (MP Biomedicals, 3mg/40g BW dissolved in 98% corn oil [Sigma] and 2% ethanol [Sigma] ...
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bioRxiv - Immunology 2020Quote: ... and the plates were blocked for 2 h at RT with 200 µL of “blocking buffer” containing PBS x1 3% BSA (MP Biomedicals) 20mM EDTA and 0.05% Tween20 (Sigma) ...
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bioRxiv - Cell Biology 2023Quote: ... 10 μM ZnSO4 and 2 mM PMSF and cells were lysed by glass bead lysis in the FastPrep-24 (MP Biomedicals). Pho8 activity was monitored by incubation of 200 μl lysate with 50 μl buffer 250 mM Tris–HCl ...
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bioRxiv - Microbiology 2023Quote: Strains were grown overnight and 1 ml of culture was transferred to a 2 ml Lysing matrix B tube (MP Biomedicals) and bead-beaten for 1 minute at 5 m/s twice ...
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bioRxiv - Immunology 2024Quote: ... DSS was offered ad libitum in drinking water at 2 percent (w/v) of DSS (molecular weight 36,000 - 50,000, MP Biomedicals, Heidelberg, Germany). The DAI is an established score to semi quantitatively score severity of experimental colitis by scoring weight loss ...
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bioRxiv - Molecular Biology 2024Quote: ... which were then scraped off of the dishes and transferred to 2 ml microcentrifuge tube containing Lysing Matrix-D (MP Biomedicals). Cells were lysed by vortexing 6 times (20 seconds each ...
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bioRxiv - Evolutionary Biology 2024Quote: ... xanthus were adjusted to ∼106 cells/mL and ∼2*1010 cells/mL respectively by resuspending in M9 buffer (M9 salts (MP Biomedicals) + 0.01mM CaCl2 + 0.2mM MgSO4) ...
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bioRxiv - Microbiology 2024Quote: ... 100 μL phosphatase inhibitor 2, and 200 μl protease inhibitor) (MSD inhibitor pack, Meso Scale Discovery) using a FastPrep Bead Beater Homogenizer (MP Biomedicals), and centrifuged (8,000 x g ...
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bioRxiv - Microbiology 2023Quote: ... with 1 min of cooling on ice in between each cycle) in 2 ml bead beating tubes (Lysing Matrix E, MP Biomedicals) using a Bead Ruptor Elite 24 (Omni International) ...
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bioRxiv - Neuroscience 2023Quote: ... the medium was changed to N2 medium supplemented with 1:2 of the day 1 supplements as well as 5 μg/ml puromycin (100552, MP biomedicals) to select for infected cells ...
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bioRxiv - Biochemistry 2022Quote: ... An equal number of cells (1-2×108 cells) was harvested and lysed using glass beads and a FastPrep Bead Beating System (MP Biomedicals). For denaturing cell lysis ...
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bioRxiv - Genetics 2022Quote: ... About 1 mm of tissue from the tips of roots were incubated in 2% cellulase Onozuka R-10 (Research Products Internationall) and 1% pectolyase Y-23 (MP Biomedicals) 37°C for 20-50 minutes to digest cell walls ...
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bioRxiv - Microbiology 2022Quote: ... and placed in 250 μls of RPMI + 2% FBS + HEPES/LN or 500 μls per foot in metal bead lysing matrix tubes (MP Biomedicals). Samples were homogenized using a Fastprep-24 (MP Biomedicals) ...
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bioRxiv - Microbiology 2024Quote: We lysed 250 mg of each travertine homogenate (2 replicates per site) by bead-beating in lysing matrix Y tubes (MP Biomedicals) with a Bead Ruptor Elite (Omni International ...
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bioRxiv - Biochemistry 2024Quote: ... 0.25 g medium glass beads and one large glass bead in a 2 mL screw cap reaction tube using a Fast-Prep-24 (MP Biomedicals) at 6 m s-1 for 30 sec ...
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bioRxiv - Immunology 2024Quote: ... A phenol/chloroform solution was added and the samples were homogenized with bead beating for 2 rounds of 30 seconds in a Fastprep24 homogenizer (MP Biomedicals) using Lysing Matrix E beads (MP Biomedicals) ...
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bioRxiv - Cancer Biology 2024Quote: ... Tumor samples stored at -80°C were transferred to Lysing Matrix A 2 mL tube containing garnet matrix and one ceramic sphere (MP Biomedicals) and minced with dissecting scissors in 500 μL of cell extraction mixture (2:3 of 70% Isopropanol ...
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bioRxiv - Microbiology 2020Quote: ... were added to the colon digesta slurry and cells were disrupted in 3 × 60 second cycles using a FastPrep24 (MP Biomedicals, Santa Ana, CA, USA). Debris were removed by centrifugation at 16.600 x g for 20 minutes and proteins were precipitated overnight in 16% ice-cold TCA ...
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bioRxiv - Microbiology 2021Quote: ... with an additional bead-beating step with ~200 mg of garnet beads at 6.0 m/s for 60 s (FastPrep-24 5G instrument; MP Biomedicals Inc., Santa Ana, CA, USA). Amplicon libraries were constructed according to the manufacture protocol from Illumina (16S Metagenomic Sequencing Library Preparation ...
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bioRxiv - Microbiology 2021Quote: Total DNA extraction from fecal samples (250 mg, wet weight) was performed using a Fast DNA SPIN Kit for feces (MP Biomedicals, Santa Ana, CA, USA), as per the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: DNA was extracted from horseshoe crab samples taken using sterile swabs and substrate samples (all stored at −80 °C) using the FastDNA™ SPIN Kit for Soil (MP Biomedicals, Santa Ana, CA) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... After blocking with 5% bovine serum albumin sections were incubated with a mouse anti-human type II collagen antibody (1:1000, clone II-4C11, MP Biomedicals, Santa Ana, CA, USA), followed by an ALP-coupled goat anti-mouse immunoglobulin G secondary antibody (ImmunoLogic ...
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bioRxiv - Plant Biology 2022Quote: ... samples of 100 mg lyophilized red fruit tissue were ground in a bead homogenizer with 1/4”-diameter ceramic spheres (MP Biomedicals, LLC, Irvine CA, USA) and vortexed in 5 mL 100% MeOH for 10 seconds ...
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bioRxiv - Microbiology 2024Quote: ... TC-DNA was extracted from 0.5 g of frozen bulk soil and from 0.5 g of frozen rhizosphere pellet (wet weight) by harsh lysis using a FastPrep-24 bead-beating system and the FastDNA Spin Kit for Soil (MP Biomedicals, Santa Ana, CA, USA) following the manufacturer instructions ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... The obtained pellets were resuspended in medium and seeded into T-75 flasks coated with 0.5 mg/mL poly-D-lysine (150175, MP Biomedicals, Santa Ana, CA, United states). Once the astrocyte-containing cells had proliferated ...
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bioRxiv - Microbiology 2024Quote: ... including an extra bead-beating step utilizing approximately 200 mg of garnet beads at a speed of 6.0 m/s for 60 seconds (FastPrep-24 5G instrument; MP Biomedicals Inc., Santa Ana, CA, USA). Library preparation and shotgun sequencing were performed at the Genome Quebec Innovation Centre (Montreal ...
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bioRxiv - Cancer Biology 2022Quote: ... Viral infections were carried out over 72 hours and transduced cells were selected by their resistance to 2 μg/mL puromycin (MP Biomedicals 0219453910).
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bioRxiv - Cancer Biology 2020Quote: ... Viral transfections were carried out over 72 hours ID8 parental cells and transduced cells were selected by their resistance to 2 μg/mL puromycin (MP Biomedicals 0219453910). Prior to use in this experiment ...
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bioRxiv - Microbiology 2021Quote: ... and bead beated 2-times for 40 sec at 6 m/sec using a FastPrep™ 24 5G Bead Beating System (MP Biomedicals). After the bead beating step ...