Labshake search
Citations for Tecan :
251 - 295 of 295 citations for Recombinant Human Low Density Lipoprotein Receptor Related Protein 1 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2024Quote: ... The supernatant was moved to a clean 96-well plate that contained 0.1% stop solution (0.1% H2SO4) and the OD at 450 nm was quantified using a plate reader (Spark, Tecan). Cells were then fixed using 4% formaldehyde in PBS for 30 min at RT and stained using Amido Black [50% Isopropanol(v/v) ...
-
Endothelial transmigration hotspots limit vascular leakage through heterogeneous expression of ICAM1bioRxiv - Immunology 2022Quote: ... Leakage and neutrophil TEM were measured simultaneously for 20 minutes with an interval of 1 minute using an Infinite F200 pro plate reader (TECAN) at 37ᵒC ...
-
bioRxiv - Cell Biology 2021Quote: ... On the day of experiment the saturated cultures were diluted 1:50 in fresh media using EVO Freedom liquid handler (Tecan) and incubated at 30°C with shaking ...
-
bioRxiv - Cell Biology 2020Quote: ... 1 ng of total RNA was used to synthesize amplified cDNA with the Ovation Pico WTA system V2 (NuGen, Tecan). Quantitative real-time PCR was performed using the KAPA SYBR Fast kit (KAPA biosystems ...
-
bioRxiv - Systems Biology 2021Quote: ... Genomic DNA was normalized to 1 ng/μL in molecular grade water using a liquid handling robot (Tecan Evo 100). Samples less than 1 ng/μL were not diluted ...
-
bioRxiv - Microbiology 2021Quote: ... 100 μL of 1 mg mL−1 Sigma 104 phosphatase substrate was added and substrate hydrolysis quantified at 405 nm using a plate reader (Spark, Tecan). To quantify immunoreactivity to T ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... the samples were incubated for 1 to 4 hours in 96 well plates before measuring absorbance at 450 nm by TECAN GENios (TECAN Group Ltd. ...
-
bioRxiv - Genomics 2022Quote: We generated single base resolution DNA methylation data using the Ovation RRBS Methyl-Seq System 1-16 (Tecan Life Sciences) with 100ng of input DNA ...
-
bioRxiv - Biophysics 2023Quote: ... Cells were back-diluted 1:100 in fresh CSM containing 2% glucose or 3% glycerol and shaken in a plate reader (Tecan) for 48 hours ...
-
bioRxiv - Biochemistry 2023Quote: ... reading fluorescence (485 nm excitation/515 nm emission) every 1 or 2 minutes with an Infinite F200 plate reader (Tecan). Relative phospholipase activity was calculated by calculating the slope of the linear part of the curve (typically in the range between 5 to 25 minutes ...
-
bioRxiv - Microbiology 2023Quote: ... Cells were then diluted 1:10 into fresh LB medium in a 96 well-plate and OD600 was measured using a plate reader (Tecan). 100 µl of the diluted culture were placed in a new 96 well plate and lysed by addition of 10 μl of Lysis Buffer (1 mg mL-1 lysozyme in 1X BugBuster buffer (Sigma) ...
-
bioRxiv - Systems Biology 2024Quote: ... Sample libraries were generated from 150 ng genomic DNA and bisulfite conversion was performed using the Ovation RRBS Methyl-Seq System 1-16 with TrueMethyl oxBS kit (Tecan) per manufacturer protocol ...
-
bioRxiv - Microbiology 2024Quote: ... After 1 h of incubation at 37 °C the release of p-nitroaniline was measured (Tecan plate reader, 405 nm). One unit (U ...
-
bioRxiv - Microbiology 2020Quote: ... chromogenic variation was measured on 1 μg of either BBMV or midgut homogenate at 405 nm (Infinite m200, Tecan, Mannedorf, Switzerland). Two different batches of BBMV were used and all enzymatic activity assays were performed in triplicates ...
-
bioRxiv - Cell Biology 2020Quote: Cell proliferation assay were performed using the WST-1 Cell Proliferation Assay Kit (ab65473) and assessed on a standard plate reader (Tecan SUNRISE)
-
bioRxiv - Biochemistry 2021Quote: ... 1 nm step interval) was recorded on black polystyrene flat-bottom 96-well plates (Costar) using a spectrofluorometer (Tecan Saphire 2). For the final fluorescence intensities ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... A stock particle solution was prepared by re-dispersing and diluting the as-prepared particles with water to achieve a final turbidity equivalent to absorbance = 1 (measured at 800 nm using a UV/Vis spectrometer, Spark 10M, TECAN, Austria). A diluted nanoparticle solution (20 μL ...
-
bioRxiv - Biophysics 2023Quote: ... The plates were incubated ∼1-2 hours at 37 °C before transfer to a Spark multimode microplate reader (Tecan, Männedorf, Switzerland) heated to 37 °C ...
-
bioRxiv - Bioengineering 2023Quote: ... For 16-weeks and ITGβ1+ / CXCR4+ sorted cells cDNA library was prepared using the whole transcriptome Ovation Solo RNA seq Library Preparation Kit (TECAN, NuGEN), followed by evaluation on an Agilent 2100 Bioanalyzer by using the DNA 1000 Chip ...
-
bioRxiv - Bioengineering 2023Quote: ... 20 μL of the washed cell suspension were incubated in 180 μL of each media (initial OD600nm of 1) in a microplate reader Infinite M200PRO (Tecan, Switzerland) and growth was monitored by measuring OD600 in 20 min intervals at 30 °C ...
-
bioRxiv - Molecular Biology 2023Quote: ... The reaction was developed for 45 min to 1 h and the absorbance was measured at 450 nm using a microplate reader (Tecan, Switzerland).
-
bioRxiv - Bioengineering 2023Quote: ... the cells were incubated with the alamarBlue™ reagent for 1 hour at 37°C and 5% CO2 before the read-out with a plate reader (Tecan). For the Trypan Blue assay ...
-
bioRxiv - Microbiology 2023Quote: ... a sample of each starting mixture was also inoculated into 96-well plate with KB (1 in 100 dilution) and incubated in a plate reader (Tecan SPARK) for 24h at 37 °C ...
-
FlhE functions as a chaperone to prevent formation of periplasmic flagella in Gram-negative bacteriabioRxiv - Microbiology 2024Quote: ... 2 µL of the antibiotic solution at the corresponding concentration were added in corresponding wells after 1 h incubation at 37°C in Tecan infinite M200 NanoQuant Plate™ (TECAN). Plates were incubated overnight at 37°C with agitation ...
-
bioRxiv - Molecular Biology 2020Quote: ... The assay was then incubated in the dark at room temperature for 1 h before the absorbance was measured at 630 nm on a multimode microplate reader (Spark, Tecan, Männedorf, Switzerland). The measurement was expressed in siderophore production unit (s.p.u.) ...
-
bioRxiv - Evolutionary Biology 2022Quote: ... Overnight cultures were diluted 1:100 and growth rate was measured by generating 24 h growth curves using an automated plate reader (TECAN infinite M200).
-
bioRxiv - Systems Biology 2022Quote: Exo-metabolomics samples from all three model organism were collected using the same protocol: 1) the biomass from the culture was separated by positive pressure filtration (Tecan Resolvex M10) using Sirocco protein precipitation filter plates (Waters ...
-
bioRxiv - Bioengineering 2023Quote: ... Emission spectra of mCherry were read in 1 nm increments from 600 to 620 nm using an Infinite M1000 (Tecan, Männedorf, Switzerland) under 580 nm excitation.
-
bioRxiv - Cancer Biology 2023Quote: ... cells were cultured in methylcellulose and treated in triplicates with serial dilutions of RMC-7977 (top concentration of 1 µM) or DMSO dispensed by a Tecan D300e digital dispenser (Tecan Trading AG). Cells were incubated for 120 hours prior to measurement of ATP levels using CellTiter-Glo ...
-
bioRxiv - Cancer Biology 2023Quote: ... Tubulin assembly was monitored for one hour at 340nm in kinetic mode of 61 cycles for each sample and the readings were recorded at an interval of 1 min (Infinite 200 PRO-Tecan plate reader).
-
bioRxiv - Systems Biology 2020Quote: ... 14.8 uL of Cline reagent was added to 185.2 uL of culture supernatant and incubated in a sealed 96-Well Microplate for 2 hours before diluting in 1% zinc acetate (Fisher) to the linear range of absorbance measurement at 667 nm (Tecan Spark Plate Reader). A standard curve was prepared similarly using sodium sulfide fixed in 1% zinc acetate ...
-
bioRxiv - Cell Biology 2020Quote: ... The samples were incubated in the dark for 1 hour at 37°C before obtaining fluorescent readings using an Infinite ® M1000 (Tecan, Männedorf, Switzerland) at 540-580 nm (excitation-emission) ...
-
bioRxiv - Plant Biology 2019Quote: ... Samples were then incubated at 95°C for 20 min with a 1% ninhydrin and 60% acetic acid reaction mix and quantified on a Tecan Infinite® 200 PRO plate reader (Tecan, Grödig, Austria) at 520 nm.
-
bioRxiv - Immunology 2020Quote: ... by adding to culture supernatant and incubating for 1 hour and reading with a plate reader (Infinite M200 Pro, TECAN Life Sciences, Switzerland) at 650 nm.
-
bioRxiv - Pharmacology and Toxicology 2022Quote: ... After 1 h, absorbance was measured (450 nm, reference wavelength 620 nm) using a plate reader (Infinite M200 Pro, Tecan group, Männerdorf, Switzerland). Absorbance was then subtracted from the reference wavelength ...
-
bioRxiv - Cell Biology 2021Quote: ... The cells were pretreated for 18 h with 1% DMSO (negative control) or the screening compounds (10 μM each) using a Fluent780® Automation Workstation (Tecan Japan, Kawasaki, Japan) with a 96-channel head adapter and Tecan sterile tips (30048824 ...
-
bioRxiv - Microbiology 2022Quote: ... Planktonic growth was measured by recording the OD600 between 30 min to 1 h intervals using a Tecan Infinite© M200 Pro spectrophotometer (Tecan Group Ltd., Switzerland) until early stationary growth phase was reached.
-
bioRxiv - Bioengineering 2020Quote: ... The lower layer of the trans-well plate was filled with various concentrations of HMGB1 (1-100 ng/ml; Tecan Group Ltd., Mannedort, Switzerland)-containing DMEM or a HMGB1-AS scaffold-containing (1000 ng HMGB1/scaffold/ml ...
-
bioRxiv - Microbiology 2023Quote: ... and transconjugants in mating populations after 6 h of growth without agitation and estimated their growth rates (h−1) based on hourly OD600 measurements (Tecan NanoQuant Infinite M200 Pro) using the R package Growthcurver ...
-
bioRxiv - Cell Biology 2022Quote: ... Substrate conversion was followed over a period of 30 minutes taking fluorescence readings at 1 min intervals using a TECAN Infinite F200 PRO machine (Tecan Trading AG, Männedorf, Switzerland) and excitation and emission wave lengths of 380 nm and 460 nm ...
-
bioRxiv - Biochemistry 2021Quote: Binding affinities of HT7D106A or HOBD106A to chloroalkane (CA) substrates were determined by fluorescence polarization (FP, equation 1) measurements using a microplate reader (Spark20M®, Tecan Group AG, Männedorf, Switzerland). The fluorescent substrates (10 nM ...
-
bioRxiv - Neuroscience 2021Quote: ... and libraries were prepared from 1 ug of total RNA using a NuGen Universal Plus mRNA-seq Library Prep Kit (Tecan Genomics Inc. Redwood City, CA). Final library products were quantified using the Qubit 2.0 Fluorometer (Thermo Fisher Scientific Inc. ...
-
bioRxiv - Plant Biology 2019Quote: ... The quantitative fungal growth inhibition by OefDef1.1 and its variants was estimated by measuring the absorbance at 595 nm using a Tecan Infinite M200 Pro (Tecan Systems Inc., San Jose, CA) microplate reader at 48 h ...
-
bioRxiv - Genetics 2021Quote: ... cDNA libraries were constructed with 1 µg of total RNA by using the “Ovation® Drosophila RNA-Seq System’’ (TECAN-NuGEN, Redwood City, CA, USA) following the manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2021Quote: ... Libraries were prepared from 1 ug of total RNA using a NuGen Universal Plus mRNA-seq Library Prep Kit (Tecan Genomics Inc. Redwood City, CA, USA). Final library products were quantified using the Qubit 2.0 Fluorometer (Thermo Fisher Scientific Inc. ...