Labshake search
Citations for Tecan :
51 - 99 of 99 citations for Dihydropyrimidinase Like Protein 2 DPYSL2 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2021Quote: ... Growth was monitored periodically (every 1-2 hours) by OD600 in a spectrophotometer with plate-reading capacity (Tecan, Switzerland). Hydrogen peroxide was added after an initial period of growth for 4 hours ...
-
bioRxiv - Microbiology 2023Quote: ... The plates were incubated at room temperature in dark for ∼2 min prior to measurement of relative luminescent units (RLU’s) using a plate reader (Tecan). ATP concentrations were determined from an ATP standard curve with ATP concentrations ranging from 1 to 1000 nM ...
-
bioRxiv - Neuroscience 2021Quote: ... Protein concentration was determined using the Quantipro BCA Protein Assay Kit (Pierce) according to the manufacturer’s protocol with a Tecan Infinite® 200 Pro (Tecan Life Sciences).
-
bioRxiv - Neuroscience 2020Quote: ... Protein concentration was determined using the Quantipro BCA Protein Assay Kit (Pierce) according to the manufacturer’s protocol with a Tecan Infinite® 200 Pro (Tecan Life Sciences).
-
bioRxiv - Biophysics 2022Quote: ... Signals were read using an excitation wavelength of 490 nm and emission detection at 523 nm in a spectrofluorometer (Safire 2, Tecan). The results (summarized in Table 1 and detailed in SI Table ...
-
bioRxiv - Biochemistry 2021Quote: ... Screening solutions were prepared and dispensed into the reservoir wells of 96×2-well MRC crystallization plates (Innovadyne Technologies) by a Freedom EVO robot (Tecan). These reservoir solutions were employed to pipet crystallization nanodrops of 100 nL each of reservoir and protein solution into the shallow crystallization wells of the plates ...
-
bioRxiv - Molecular Biology 2022Quote: ... 120 µL of clear lysates were mixed with 2 µL of HEPES buffer or 2 µL of 30 mM cAMP or 2 µL of 30 mM cGMP and then the fluorescence were recorded with an Infinite M1000 PRO fluorometer (Tecan). The fluorescence change ΔF/F0 was calculated as (F-F0)/F0 ...
-
bioRxiv - Neuroscience 2022Quote: The linear optical properties of the GRAB peptide sensors expressed in HEK293T cells were measured using a Safire 2 plate reader (Tecan). Cells were harvested and transferred to black-wall 384-well plates containing either saline alone or saline containing the corresponding peptides ...
-
bioRxiv - Biophysics 2019Quote: ... Fluorescence emission spectra were measured for each sample from 300 nm to 450 nm (excitation at 295 nm and step size of 2 nm) using an Infinite M1000 plate reader (Tecan). Fluorescence was integrated and converted into fraction of unfolded protein values ...
-
bioRxiv - Neuroscience 2020Quote: ... Fluorescence intensity of the Cy7-labelled ALP or FITC-angiopep-2 was measured in black 96-well plates using a Spark Multimode microplate reader (Tecan). Apparent permeability was calculated using Equation 1.
-
bioRxiv - Microbiology 2021Quote: ... 100 μL of heat-activated spores were then added and the OD600 was monitored every 2 min for 4 h using Infinite M Plex plate reader (Tecan). The 96-well plate was maintained at 37° C with agitation between measurements ...
-
bioRxiv - Biochemistry 2022Quote: ... Screening solutions were prepared and dispensed into the reservoir wells of 96×2-well MRC crystallization plates (Innovadyne Technologies) by a Freedom EVO robot (Tecan) using the sitting-drop vapor diffusion method ...
-
bioRxiv - Molecular Biology 2022Quote: ... Reactions were started by addition of ATP-MgCl2 to a final concentration of 2 mM and fluorescence readings taken in an InfinitePro M200 (Tecan) or Spark (Tecan ...
-
bioRxiv - Molecular Biology 2022Quote: ... eIF4A (25 µM final concentration) was added to the reaction and fluorescence was measured using a Safire 2 microplate reader (Tecan).
-
bioRxiv - Microbiology 2023Quote: ... ROS production was determined as a function of oxidation of the non- fluorescent DCF-H to 2′,7′-dichloroofluorescein (DCF) in an Infinite M PLEX plate reader (Tecan) with the following settings ...
-
bioRxiv - Biochemistry 2023Quote: ... reading fluorescence (485 nm excitation/515 nm emission) every 1 or 2 minutes with an Infinite F200 plate reader (Tecan). Relative phospholipase activity was calculated by calculating the slope of the linear part of the curve (typically in the range between 5 to 25 minutes ...
-
bioRxiv - Cancer Biology 2023Quote: ... plates were mixed for 2 min using the linear shaking function of a Tecan Infinite 200 PRO plate reader (Tecan), then incubated for 10 min at room temperature before measuring well luminescence using Tecan plate reader ...
-
bioRxiv - Biophysics 2023Quote: ... Cells were back-diluted 1:100 in fresh CSM containing 2% glucose or 3% glycerol and shaken in a plate reader (Tecan) for 48 hours ...
-
bioRxiv - Cancer Biology 2024Quote: ... Stained cells were lysed in 100 µl 2% SDS and absorbance at 590 nm was measured using an Infinite M200Pro plate reader (Tecan).
-
bioRxiv - Biochemistry 2024Quote: ... Assays were initiated by the addition of 2 mM RuBP and reaction progress was followed in a microplate reader (Tecan) at 25 °C by measuring the consumption of NADH ...
-
bioRxiv - Microbiology 2024Quote: ... Fluorescence was measured at 28°C every 2 min for a total of 100 min incubation in an infinite M1000 PRO fluoresce nce plate reader (Tecan), operated with Tecan i-control ...
-
bioRxiv - Biophysics 2022Quote: ... Incubation for 10 minutes led to a stable protein–dye complex that was monitored at 595 nm using a spectrophotometer TECAN infinite F200 PRO (Tecan Trading AG, Switzerland). The vesicle protein amount was calculated using a BSA calibration curve in a range of 0.05–2 mg/mL ...
-
bioRxiv - Cell Biology 2022Quote: ... and quantitated using Thermo Fisher Scientific micro BCA™ Protein Assay kit (catalog no. 23235) according to the manufacturer’s instruction using the Tecan microplate reader (Tecan Infinite 200 Pro).
-
bioRxiv - Microbiology 2023Quote: ... the amount of cell-free synthesized GFP protein was estimated by measuring the florescence intensity using Spark multimode microplate reader (Tecan Trading AG, Switzerland).
-
bioRxiv - Biochemistry 2024Quote: Turbidity of protein samples was collected by measuring the absorbance at 350 nm in a TECAN plate reader (Tecan Group Ltd., Mannedorf, Switzerland). All samples reached a final volume of 50 μL in a 364-Well Flat-Bottom Microplate (UV-Star ...
-
bioRxiv - Biochemistry 2021Quote: ... 1 nm step interval) was recorded on black polystyrene flat-bottom 96-well plates (Costar) using a spectrofluorometer (Tecan Saphire 2). For the final fluorescence intensities ...
-
bioRxiv - Genomics 2021Quote: ... Each ligation was then split into 2 aliquots to perform oxidation + bisulfite conversion or mock (water) + bisulfite conversion according to the OxBS module instructions (Tecan/NuGen). PCR amplification was performed using NEB’s dual-indexing primers and KAPA Uracil+ HiFi enzyme for a total of 10 cycles ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... Colour development was halted after 10 mins by the addition of HsSO4 (2 M) and quantified at λabs = 450 nm using a Tecan Infinite M200 Pro multi-well plate reader (Tecan Group). Specific binding was determined by subtracting the absorbance measured in samples lacking ACE2.
-
bioRxiv - Immunology 2023Quote: ... stopped with 0.2 M H2SO4 solution and plates read at 450 nm with 620 nm background correction using an ELISA reader (Tecan Sunrise).
-
bioRxiv - Biophysics 2023Quote: ... The plates were incubated ∼1-2 hours at 37 °C before transfer to a Spark multimode microplate reader (Tecan, Männedorf, Switzerland) heated to 37 °C ...
-
The activator domain of bacterial collagenases drives collagen recognition, unwinding and processingbioRxiv - Biochemistry 2023Quote: ... Cleavage of the substrate was monitored for 2 min at 25 °C (excitation: 328 nm, emission: 392 nm) in an Infinite M200 plate reader (Tecan, Austria) and the initial velocity (v0 ...
-
bioRxiv - Immunology 2023Quote: ... stopped with 0.2 M H2SO4 solution and plates read at 450 nm with 620 nm background correction using an ELISA reader (Tecan Sunrise).
-
bioRxiv - Plant Biology 2020Quote: ... Glucose concentration was measured with a colorimetric method by developing color in Anthrone reagent (freshly prepared 2 mg/mL anthrone in concentrated sulfuric acid) and reading OD625 nm in a plate reader (Tecan Infinite 200Pro). Nine repeats were performed for each treatment ...
-
bioRxiv - Immunology 2022Quote: ... the plates were transferred to the BSL-3+ Caps-It robotics system where 30 µL SARS-CoV-2 was added using a noncontact liquid handling system (Tecan, EVO 100) to achieve a multiplicity of infection (MOI ...
-
bioRxiv - Molecular Biology 2022Quote: ... Reactions were incubated for 30 min at 37 °C before being neutralized by the addition of 50 μL of 2 M NaOH and absorption at 405 nm immediately measured (Tecan Infinite M1000). A405 were blanked to reactions containing naïve lysis buffer ...
-
bioRxiv - Genetics 2023Quote: We quantified the growth rates of each hybrid in liquid synthetic complete (SC) media with 2% glucose for 48 hours (initial OD600nm: 0.1) using a 96-well microplate reader (Tecan Infinite F200 Pro). The hybrids were then grown in 96-format deepwell plates until mid-log phase (OD600nm ∼0.3) ...
-
bioRxiv - Physiology 2023Quote: Media was again replaced with fluorobrite imaging media supplemented with 2 mM Sodium Butyrate and red and green fluorescence was measured in a plate reader (Tecan Spark600 Cyto), this measurement was T= 0 h ...
-
bioRxiv - Bioengineering 2024Quote: ... plates were incubated at 37 °C under 5% CO2 for 2 hours before measuring the MTS absorbance at 490 nm using a microplate reader (TECAN Spark 20M).
-
bioRxiv - Cell Biology 2020Quote: ... The assay reaction was performed for 2-4 hour at 37 °C and the activity was kinetically measured with a fluorescence microplate reader (Tecan Infinite 200 Pro) at excitation and emission wavelengths of 530 nm and 585 nm ...
-
bioRxiv - Systems Biology 2020Quote: ... 14.8 uL of Cline reagent was added to 185.2 uL of culture supernatant and incubated in a sealed 96-Well Microplate for 2 hours before diluting in 1% zinc acetate (Fisher) to the linear range of absorbance measurement at 667 nm (Tecan Spark Plate Reader). A standard curve was prepared similarly using sodium sulfide fixed in 1% zinc acetate ...
-
bioRxiv - Microbiology 2021Quote: Reduced susceptibility to CHX was assessed by measuring bacterial growth in LB supplemented with 2 mg/L CHX over a 24h period with an automatic reading of optical density (Tecan Infinite F200 pro) at 600 nm as previously described (La Combe et al. ...
-
bioRxiv - Cell Biology 2023Quote: ... Cells were cultured in 1.2 l of SC + 2% glucose overnight to early exponential growth phase (0D600=0.14 measured using a Tecan Infinite M1000 Pro Plate Reader), and centrifuged in a Sorvall SLC-6000 rotor at 1100 rpm for 10 min at 4°C ...
-
bioRxiv - Neuroscience 2023Quote: ... The assay reaction was performed for 2-4 hour at 37 °C and the activity was kinetically measured with a fluorescence microplate reader (Tecan Infinite 200 Pro) at excitation and emission wavelengths of 530 nm and 585 nm ...
-
bioRxiv - Synthetic Biology 2019Quote: ... The level of antibody binding was measured as optical density in a GENios Microplate Reader (Tecan, Männedorf, Switzerland) at 405 nm.
-
bioRxiv - Biochemistry 2020Quote: ... Secondary antibody (Anti-Human IgG-Cy3, Jackson ImmunoReserarch) was used for fluorescence detection and microarrays scanned by TECAN power scanner at 10% laser intensity and 50% gain.
-
Engineering synthetic CopT/A-based genetic circuits for miRNA imaging and functional gene regulationbioRxiv - Synthetic Biology 2023Quote: ... Then the OD value of each well was measured at the filter of 450 nm through a microplate reader (Tecan Safire 2, Männedorf, Switzerland) and the cell viability was calculated ...
-
bioRxiv - Cell Biology 2022Quote: ... PA was then measured using the Human Phosphatidic Acid Antibody (IgM ELISA Kit (Lifeome Bioloabs) following the manufacturers protocol and monitoring fluorescence on a Tecan F250Pro microplate reader (Tecan).
-
bioRxiv - Genomics 2022Quote: ... and IL-8 levels were quantified from streptavidin-Horseradish peroxidase associated with the detection antibody using absorbance read by an Infinite M1000 plate reader (Tecan).
-
bioRxiv - Plant Biology 2021Quote: ... RLU) was measured every 2 min during 46 or 720 min with a Tecan Infinite F200 PRO (or a TECAN CM SPARK for Supplementary figure 6), Tecan France ...