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Citations for PNA Bio :
101 - 124 of 124 citations for Recombinant Human CD22 protein His tagged R PE labeled since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... containing 10 g of each sgRNA (#1 and #2 modified sgRNAs, Synthego) and 15 g Cas9 protein (PNA Bio) and were electroporated using program A23 on the Nucleofector™ 2b Device (Lonza) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... we used a non-homologous end joining mediated strategy by injecting the mixture of Cas9 protein (#CP01; PNA Bio) and sgRNAs into the embryos of this species ...
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bioRxiv - Developmental Biology 2021Quote: ... or with 5 nl or 10 nl of 75pg/nl of sgRNA mixed with 0.2 ng/nl of Cas9 protein (PNA Bio) and 0.1% Texas Red Dextran in 1 cell of 2-cell stage embryos ...
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bioRxiv - Cell Biology 2022Quote: ... 80 pg of sgRNA was co-injected with 200 pg of Cas9 protein (#CP01-50; PNA Bio, Newbury Park, CA) into 1-cell zebrafish embryos ...
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bioRxiv - Molecular Biology 2023Quote: ... 1 µL of annealed RNAs was incubated with 1 µL of 10 mg/mL Cas9 protein (PNA Bio # CP01-200) at room temperature (22°C ...
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bioRxiv - Cell Biology 2023Quote: ... and 1.65µL of each of two lmn-1 crRNA per strain (30pmol/µl) was combined and added to 1µL of purified Cas9 protein (5µg/µl from PNA Bio, Inc). Following addition of 1µL of the dpy-10 ssODN (500ng/µL ...
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bioRxiv - Developmental Biology 2021Quote: Mutants were generated by injecting one-cell stage embryos with 200 ng/ml sgRNAs and 500 ng/ml Cas9 protein (PNA Bio) using standard procedures (Shah et al. ...
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bioRxiv - Developmental Biology 2021Quote: ... mutations we used CRISPR/Cas9 mutagenesis by injecting 1-cell stage embryos with 200 ng/μl T7 sgRNA and 500 ng/μl Cas9 protein (PNA Bio) as described (Shah et al. ...
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bioRxiv - Developmental Biology 2020Quote: ... esrp2 and irf6 CRISPR sgRNAs were generated by in vitro transcription from a SP6 promoter as described.(Gagnon et al., 2014) Lyophilized Cas9 protein (PNA Bio) was resuspended in ddH2O to a stock concentration of 1 μg/μl and stored in single-use aliquots in −80°C to avoid freeze-thaw inactivation and kept for 6 months ...
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bioRxiv - Developmental Biology 2022Quote: ... In vitro transcribed guide RNAs were made from PCR-generated templates (Bhattacharya et al. 2015) and were complexed with Cas9 protein (CP01; PNA Bio) at 37°C before injection into the animal pole of fertilised eggs at the 1-2 cell stage ...
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bioRxiv - Genetics 2020Quote: ... An injection mix was made with 40 ng/ul sgRNA RNA and 300 ng/ul Cas9 protein (PNA Bio # CP01-50) and injected into embryos from our BDC stock (Fig ...
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bioRxiv - Neuroscience 2022Quote: ... embryos at the very early 1 cell stage were injected with 374.5 ng/μl of the guide RNA targeted to zebrafish klc4 complexed with 800 ng/μl of Cas9 protein with NLS (PNA Bio). Injection volume was approximately 1nL ...
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bioRxiv - Developmental Biology 2021Quote: ... we injected a mixture of guide RNA (200pg/nl total final concentration) and Cas9 protein (PNA Bio; 400 pg/nl final concentration) into one-cell stage AB embryos ...
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bioRxiv - Cell Biology 2023Quote: ... CD33 chemically modified guide RNAs (6.25 μg/106 cells, Synthego, Redwood City, CA) and Cas9 protein (12.5 μg/106 cells, PNA Bio, Thousand Oaks, CA) were mixed for 10 minutes at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... Fertilised eggs from C57BL/6J mice were injected with the two gRNAs (Msh3 gRNA 51/57 and Msh3 gRNA 89/69) and Cas9 protein (PNA BIO INC) and transferred to pseudopregnant CD1 female mice ...
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bioRxiv - Developmental Biology 2021Quote: A mixture of the pooled guide RNA cocktail (400pg/μl total final concentration) and Cas9 protein (PNA Bio; 200 pg/μl final concentration) was injected into one-cell stage AB embryos ...
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bioRxiv - Neuroscience 2022Quote: ... Wild type embryos of the Aedes aegypti Liverpool strain were injected at the Insect Transformation Facility at the University of Maryland Institute for Bioscience and Biotechnology Research with a gene-targeting mixture composed of 300 ng/μL Cas9 protein with NLS (PNA Bio, CP01-200) and 4 sgRNAs ...
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bioRxiv - Biophysics 2023Quote: ... ribonucleoprotein (RNP) complexes included sgRNAs targeting either N- or C-terminal region (Integrated DNA Technologies - IDT) and Cas9 protein (PNA bio, Cat #CP01) were transfected together with linear dsDNA donors (IDT ...
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bioRxiv - Genetics 2019Quote: ... and trans-activating crRNA (tracrRNA) (IDT) or single guide RNAs (sgRNA) transcribed in vitro were combined with Cas9 protein (PNA Bio Inc. or IDT) to generate complexes used for genome editing ...
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bioRxiv - Genetics 2022Quote: ... and the capillary was injected with an injection mixture containing sgRNA (500 ng/ul) and Cas9 protein (CP-01, PNA Bio; 500 ng/ul) (injection pressure Pi 100 Pa ...
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bioRxiv - Developmental Biology 2023Quote: Verification of in vitro cutting was confirmed by adding 250 ng of each sgRNA and 500 ng of Cas9 protein (PNA Bio cat. CP01-200) to 250 ng of PCR-amplified Cte-chd-l and incubating at RT for 1 h ...
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bioRxiv - Bioengineering 2020Quote: ... Mutation rate for each guide was determined by laser-assisted cytoplasmic injection37 of in vitro fertilized embryos with 6pL of a solution containing 67ng/μL of in vitro transcribed gRNA alongside 133ng/μL of Cas9 mRNA or 167ng/μL of Cas9 protein (PNA Bio, Inc., Newbury Park, CA) incubated at room temperature for 30 minutes prior to injection ...
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bioRxiv - Bioengineering 2020Quote: Mutation rate for each guide was determined by laser-assisted cytoplasmic injection36 of in vitro fertilized embryos with 6pL of a solution containing 67ng/μL of in vitro transcribed gRNA alongside 133ng/μL of Cas9 mRNA or 167ng/μL of Cas9 protein (PNA Bio, Inc., Newbury Park, CA) incubated at room temperature for 30 minutes prior to injection ...
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bioRxiv - Developmental Biology 2024Quote: ... co-injected one-cell stage embryos with RNPs containing 25 pg of gRNA and 500 pg of NLS-Cas9 protein (PNA Bio, Thousand Oaks, CA USA), and raised these fish to adulthood ...