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Citations for Expedeon :
101 - 131 of 131 citations for Probable protein BRICK1 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... The supernatant was then mixed with 100 μl slurry of Protein A/G Sepharose beads (Expedeon, AGA1000) that had been pre-washed in Cell lysis buffer ...
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bioRxiv - Neuroscience 2022Quote: ... and the purity was verified by SDS-PAGE and total protein staining using instant blue reagent (Expedeon). The AAV was administered to 30 days old male C57BL6 mice or PDGFRβ-Cre x RCL-GCaMP6 mice with a retro-orbital injection (150 μl ...
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bioRxiv - Developmental Biology 2022Quote: The concentration of total protein extracted from animal organs was quantified using BradfordUltra (Expedeon Ltd, Cambridgeshire, UK). Antibodies (Abs ...
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bioRxiv - Plant Biology 2021Quote: ... The purity of proteins was judged by running 16% SDS-PAGE gels and staining with InstantBlue™ (Expedeon). PexRD54 was purified as described previously (19).
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bioRxiv - Bioengineering 2020Quote: ... Gels were stained for at least 4 hours using InstantBlue Protein Stain (Expedeon Inc., San Diego, CA, USA), and destained O/N using demineralized water ...
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bioRxiv - Molecular Biology 2020Quote: ... The protein fractionation step was performed with a Gel Elution Liquid Fraction Entrapment Electrophoresis (Gelfree 8100) system (Expedeon). Nab3-containing fractions were treated with 100 μg of proteinase K (Roche ...
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bioRxiv - Cancer Biology 2020Quote: ... Then antibody was labelled with R-Phycoerythrin using Lightning-Link kit (Expedeon). All antibodies were used at concentrations between 1/50 and 1/200 depending on specificity and cell density ...
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bioRxiv - Microbiology 2021Quote: ... The final concentration was determined by SDS-PAGE using a BSA standard and visualized by InstantBlue protein stain (Expedeon).
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bioRxiv - Cancer Biology 2021Quote: ... 20-25 µg of proteins were prepped using the filter-aided sample preparation (FASP) method (Expedeon, San Diego, CA). Samples were reduced with 10 mM DTT for 30 min at RT ...
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bioRxiv - Synthetic Biology 2020Quote: ... and separated by 8-15% SDS-PAGE (0.05 OD equivalent/lane) and stained with Instant Blue Coomassie Protein Stain (Expedeon).
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bioRxiv - Genetics 2020Quote: ... the purified FAM19A5 was labeled with Alexa Fluor 488 or Cy3 using a protein labeling kit (Expedeon, United Kingdom), according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: ... 102235) and conjugated per antibody using a ThunderLink kit (Expedeon cat. 425-0000) to distinct 20 bp 3’ amine-modified HPLC-purified oligonucleotides (IDT ...
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bioRxiv - Immunology 2021Quote: ... and conjugated in house using Thunder-Link Plus Oligo Antibody conjugation kits (Expedeon) in accordance with the manufacturer’s instructions using an antibody:oligo ratio of 1:5 ...
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bioRxiv - Molecular Biology 2019Quote: ... The bound protein complex was eluted with LB supplemented with 5 mM desthiobiotin and analyzed by SDS-PAGE and InstantBlue (Expedeon) staining.
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bioRxiv - Biochemistry 2019Quote: ... Gels were scanned for fluorescence on a GE Typhoon FLA 9500 using a green (λex/em 473/530 nm) and red (λex/em 532/570 nm) channel followed by staining with InstantBlue Coomassie protein stain (Expedeon) after which the gel was scanned on a GE Amersham Imager 600.
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bioRxiv - Microbiology 2021Quote: ... an identical amount of proteins has been deposited on another SDS-PAGE run in parallel and stained with the Coomassie blue InstantBlue protein staining (Expedeon). The bioluminescence signal (MreB ...
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bioRxiv - Microbiology 2021Quote: ... gels supplemented with 25 μM PhosTag reagent and 50 μM MnCl2 and the protein was visualized using InstantBlue Coomassie stain (Expedeon).
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bioRxiv - Molecular Biology 2022Quote: ... The protein fractionation step was performed with a Gel Elution Liquid Fraction Entrapment Electrophoresis (Gelfree 8100) system (Expedeon, Cat# 48100). Final fractions were treated with 100 μg of proteinase K (Roche ...
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bioRxiv - Molecular Biology 2019Quote: ... Bound protein was eluted in LB supplemented with 250 mM Imidazole and subsequently analyzed by SDS-PAGE and InstantBlue (Expedeon) staining.
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bioRxiv - Cell Biology 2019Quote: ... Fractions containing the eluted protein were run on an SDS-PAGE gel and the purity of recombinant Sgo1 was assessed by InstantBlue (Expedeon) staining.
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bioRxiv - Cell Biology 2020Quote: ... Purity control was made by running samples on SDS-PAGE followed by coomassie blue staining (InstantBlue™ Protein Stain, Expedeon). For experiments in Figure 4 and 5 ...
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bioRxiv - Biochemistry 2021Quote: ... Approximately 500,000 cell equivalents or 25 μg of protein was loaded into each well of ClearPAGE 4-20% polyacrylamide gradient gels (Expedeon) and resolved by denaturing polyacrylamide gel electrophoresis at 100 Volts for 1 h ...
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bioRxiv - Microbiology 2022Quote: ... acrylamide gels supplemented with 25 μM PhosTag reagent and 50 μM MnCl2 and the protein was visualized using InstantBlue Coomassie stain (Expedeon). To measure the ratio of CERTO to total CERT ...
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bioRxiv - Cell Biology 2023Quote: ... Protein (30 µg) was separated on a precast 4–12% gradient or 12% np gradient SDS-PAGE gel (Expedeon, NBT41212) and transferred to immobilon PVDF (Millipore ...
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bioRxiv - Biochemistry 2023Quote: ... was performed and the purity of GSEC complexes was assessed by SDS-PAGE and Coomassie staining (InstantBlue Protein Stain, Expedeon).
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bioRxiv - Microbiology 2021Quote: ... and equal amount of proteins per sample (13 μg) were loaded on a pre-casted RunBlue™ TEO-Tricine gel (Expedeon) in presence of LDS and DTT ...
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bioRxiv - Biochemistry 2021Quote: ... The purified proteins were analyzed by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) on a 15% polyacrylamide gel under reducing conditions followed by staining with InstantBlue Coomassie Protein Stain (Expedeon, Cambridge, UK). Finally ...
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bioRxiv - Cancer Biology 2022Quote: ... A549-Src-KO cells served as a non-specific binding control The protein mixtures were loaded on a RunBlue 1 mm*10 well 8%-Bis-Tris – gel (Expedeon, Cambridge, UK) after heating the mixture at 70°C for 10 min ...
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bioRxiv - Microbiology 2022Quote: ... The samples were then centrifuged at 15000 g for 10 min and Filter Aided Sample Preparation (FASP) (65) kits were used for protein digestion (Expedeon, San Diego, CA) according to the manufacturer’s instructions ...
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bioRxiv - Pathology 2023Quote: ... and a secondary antibody coupled to a synthetic DNA fragment was added following manufacturer’s instructions (Thunder-Link Plus Oligo Conjugation System, Expedeon). After 2 washing cycles ...
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bioRxiv - Systems Biology 2021Quote: ... A maximum of 1×106 cells/donor were stained by oligo-barcoded antibodies (TotalSeq-A or TotalSeq-C; Biolegend, Expedeon) for 30 min on ice ...