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Citations for Sartorius :
51 - 100 of 101 citations for VEGF C Human 196a.a HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... Eluted total IgG was concentrated by centrifugation at 4°C for 30 mins using 100000MWCO vivaspin (Sartorius). The concentrate was then dialyzed using a dialysis tube (Millipore ...
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bioRxiv - Biophysics 2022Quote: ... Dialyzed protein was then concentrated with 10 kDa MWCO Vivaspin centrifugal concentrators (3,500 xg, 4°C) (Sartorius). Protein concentrations were determined by means of fluorescence using an sfGFP calibration curve ...
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bioRxiv - Biochemistry 2023Quote: ... Samples were then diafiltered at 4°C using 3 kDa cutoff VivaSpin®500 centrifuge filters (Sartorius) for 3 cycles of 20 min at 14,000 rpm ...
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bioRxiv - Microbiology 2023Quote: ... which took place overnight on arrival at 4°C (cold room) using 6 Vivaflow200 filtration units (Sartorius) operating in two parallel series ...
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bioRxiv - Microbiology 2022Quote: ... and 0.5% nonfat dry milk) to 100 nM and immobilized for 60 s to anti-human IgG Fc capture biosensors (Sartorius). Sensors were transferred to assay buffer for 30 s to set the baseline ...
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bioRxiv - Biophysics 2021Quote: ... at pH 7 via centrifugal filter units at 12000 g at 4°C (Vivaspin 500, MWCO 10000, Sartorius). Glycans for native MS analysis were mixed with 1 µM (per monomer ...
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bioRxiv - Cell Biology 2022Quote: ... cells were placed in the Incucyte SX5 and maintained at 37°C and 5% CO2 (Sartorius, Göttingen, Germany) for detection and automated analysis of cell growth/proliferation ...
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bioRxiv - Synthetic Biology 2022Quote: ... Co-cultures were incubated at 37°C and imaged with an Incucyte S3 Live-Cell Analysis System (Sartorius) for approximately 72 hours ...
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bioRxiv - Immunology 2023Quote: All BLI for data shown in Figure 1 and Figure S1 was carried out on an OctetRED384 (ForteBio) using anti-human Fc-capture sensors (Sartorius, 18-5060) to immobilize anti-PfRH5 mAbs ...
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bioRxiv - Biochemistry 2020Quote: ... oxidized BdiGolSase1 was ultrafiltrated by centrifuging at 10,000 x g and 4°C using a Vivaspin 500 device (Sartorius). To evaluate the reversibility of the process ...
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bioRxiv - Biophysics 2020Quote: ... 250 mM) at pH 7.5 and 4°C via centrifugal filter units (13000 x g, Vivaspin 500, MWCO 10000 (Sartorius) or Micro Bio-Spin 6 columns (Bio-Rad) ...
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bioRxiv - Bioengineering 2022Quote: ... the appropriate volume of stock solution was centrifuged at 3000g at 10 °C in Vivaspin® 20 concentrators (Sartorius Stedim Biotech GmbH ...
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bioRxiv - Microbiology 2020Quote: ... Reactions were incubated for 16 h at 37 °C following removal of RiGH26 using a Vivaspin 20 filtration unit (10.000 MWCO PES, Sartorius) and carbohydrate lyophilization on an ALPHA 2-4 LD Plus freeze dryer (Christ ...
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bioRxiv - Cell Biology 2019Quote: Live-cell imaging was performed at 37°C under humidified 5% CO2 atmosphere using the IncuCyte Zoom system (Sartorius). Phase and fluorescence images were also acquired using Leica DiM8 and Zeiss LSM710 microscopes ...
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bioRxiv - Molecular Biology 2022Quote: ... were concentrated to 2 ml by centrifugation 30 min at 4,000xg at 4°C through a 20 ml VivaSpin 20 30 kDa MWCO concentrator (Sartorius, and were diluted with TGE containing 1 mM β-mercaptoethanol to adjust the NaCl concentration to 50 mM ...
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bioRxiv - Cancer Biology 2023Quote: ... at a final concentration of 31.3 ng/mL for 45 minutes at 37°C in pHrodo Labeling Buffer (Sartorius), before being washed again and resuspended in complete RPMI medium ...
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bioRxiv - Microbiology 2023Quote: ... Supernatants were thawed and filtrated through a 0.2 µm filter before centrifugation at 4500 x g at 4°C in VivaSpin tubes (Sartorius). The first-generation immunoadhesins do not carry the Twin-Strep-tag and were purified using Protein A Mag Sepharose™ Xtra beads (50 µL beads/mL of supernatant ...
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bioRxiv - Biochemistry 2023Quote: ... Samples were incubated at room temperature during one hour and labeled proteins were separated from Ir2 and DMSO vehicle by 5 cycles of diafiltration (12,000 g, 4°C, molecular cutoff 10kDa, Sartorius) adding 5 volumes of labeling buffer before each cycle ...
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bioRxiv - Immunology 2023Quote: ... Cells were washed once in PBS and kept in 500μL PBS at 4°C in the dark until analysis with an Intellicyt iQue3 Screener (Sartorius). For viability analysis ...
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bioRxiv - Microbiology 2024Quote: ... The lysate was cleared by centrifugation at 35,000 rcf for 30 minutes at 4°C followed by filtering with a GF filter (Sartorius). The cleared lysate was incubated with 3 mL of Glutathione Sepharose 4B resin (Cytiva ...
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bioRxiv - Cell Biology 2024Quote: ... The plate was incubated at 37°C and 5% CO2 and imaged using an Incucyte Live-Cell imager (Sartorius). The red channel used an acquisition time of 400 milliseconds for all replicates and samples ...
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bioRxiv - Microbiology 2024Quote: ... The lysate was cleared by centrifugation at 35,000 rcf for 45 minutes at 4°C followed by filtering with a GF filter (Sartorius). The cleared lysate was incubated with 2 mL Ni-NTA agarose (Qiagen ...
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bioRxiv - Microbiology 2024Quote: ... The lysate was cleared by centrifugation at 70,000 rcf for 45 minutes at 4°C followed by filtering with a GF filter (Sartorius). The cleared lysate was incubated with 4 mL of IgG beads (Cytiva ...
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bioRxiv - Microbiology 2024Quote: ... Lysates were cleared by centrifugation at 35,000 rcf for 45 minutes at 4°C followed by filtering with a GF filter (Sartorius). Cleared lysates were then incubated with 1.5 mL of StrepTactin Sepharose beads (IBA Lifesciences ...
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bioRxiv - Microbiology 2024Quote: ... Lysates were cleared by centrifugation at 35,000 rcf for 30 minutes at 4°C followed by filtering with a GF filter (Sartorius). For purification of ScaC and ScaC-SNAP ...
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bioRxiv - Biochemistry 2023Quote: ... The experiments were conducted at 20°C. The resulting data were processed using Octet Analysis Studio (ver. 13.0)(Sartorius) and reference biosensor (loaded with GST-only ...
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bioRxiv - Cell Biology 2022Quote: ... Lysates were spun at 15,000g for 15 min at 4°C and supernatants were then passed through a regenerated cellulose filter (4mm Ø, Sartorius). Samples were then dried under N2 flow at 40°C and resuspended in 125µl of ice-cold methanol/water 70:30 for subsequent analyses.
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... The resulting peptide solutions were centrifuged at 13,000 rpm for 15 min at 4 °C on tubes equipped with 2000 MW filtrate tubes (Sartorius, USA). Then ...
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bioRxiv - Biophysics 2022Quote: BLI analyses were performed at 25°C at an acquisition rate of 5 Hz averaged by 20 using an Octet© BLItz System (FortéBio/Sartorius) with assays were designed and setup using Blitz Pro 1.3 Software ...
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bioRxiv - Microbiology 2021Quote: ... the cells were washed three times with PBS and incubated in growth medium at 37°C in an IncuCyte S3 Live-Cell Analysis System (Sartorius). At the indicated times post infection ...
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bioRxiv - Neuroscience 2020Quote: ... cells and media were collected and this mixture was centrifuged at 3700xg at 4°C to remove debris and then concentrated by ultrafiltration using Vivaflow 200 (Sartorius). The purified AAV particles were collected after running the viral concentrate through an iodixanol column (Opti-Prep density gradient medium ...
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bioRxiv - Immunology 2021Quote: ... The purified Spike and RBD recombinant proteins were then buffer exchanged into sterile PBS by centrifuging at 4000xg for 30 minutes at 4 °C in a 10,000 MWCO Vivaspin centrifugal concentrator (Sartorius, Germany) and stored at –80 °C ...
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bioRxiv - Immunology 2023Quote: ... The cocultures were incubated for 48 hours in a humidified incubator (37 °C, 5% CO2) equipped with an Incucyte (Sartorius) for continuous monitoring of cell proliferation and target cell killing ...
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bioRxiv - Biochemistry 2020Quote: ... 4 °C) culture medium from two 90% confluent 15 cm dishes was filtered through a 1.2 μm filter (Sartorius Stedim Biotech) and applied to a HisTrap column (1 ml bed volume ...
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bioRxiv - Cancer Biology 2023Quote: ... The plates were spun down at 350g for 10 min at 4°C and placed in the Incucyte® Live-Cell Analysis System (Sartorius). The growth of the spheroids was monitored for the following 8 days and invasion was automatically quantified via the Incucyte® Spheroid module analysis tool ...
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bioRxiv - Plant Biology 2023Quote: ... and dried for at least 72 hrs at 70°C for subsequent dry mass measurement (resolution: 0.001 g; Sartorius, Göttingen, Germany). The other leaf was used for anatomical measurements ...
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bioRxiv - Neuroscience 2024Quote: ... Lysates were spun at 15,000g for 5 min at 4°C and supernatants were passed through a 4 mm regenerated cellulose filter (4 mm Ø, Sartorius). Samples were then dried under nitrogen flow at 40°C and resuspended in 125 µl of methanol/water 70:30 for subsequent analyses.
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bioRxiv - Microbiology 2019Quote: ... viruses were concentrated by centrifugation at 4 °C using vivaspin 20 concentrators with a molecular weight cutoff of 100K (Sartorius, Göttingen, Germany). Thereafter ...
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bioRxiv - Physiology 2019Quote: ... were inoculated with 2 mL of stock culture and grown at 30°C and at 200 rpm for 8 h in a B Braun Certomat BS-1 incubator (Sartorius, Melsungen, Germany). Bioreactor batch cultures were started by transferring 400 mL of preculture to a bioreactor containing 4.6 L of medium ...
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bioRxiv - Biochemistry 2020Quote: ... and incubated at 37°C with 5% CO2 during 24h in the IncuCyte S3 live-cell imaging and analysis system (Essen BioScience, Sartorius, Göttingen, Germany). Images were acquired using a 10X objective and 440-480 nm Excitation / 504-544 nm Emission filters ...
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bioRxiv - Animal Behavior and Cognition 2021Quote: ... we dried the samples at 70°C for 24 h and weighed them twice to the nearest microgram (CPA26P, Sartorius, Göttingen, Germany). We transferred the samples into 15 ml round-bottom glass test tubes (16×150 mm ...
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bioRxiv - Microbiology 2020Quote: ... liquid phage suspension was centrifuged at 4000 g for 10 min at 4°C and the supernatant was subsequently filter sterilised using a 0.45 μm pore sized membrane filter (Sartorius Minisart®, 16555). The filtrate phage lysate was stored at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... RKO cells were then incubated for 72 hrs at 37°C in an IncuCyte S3 live imaging incubator (Sartorius and Essun BioScience). Each well was imaged in five locations with a 10X objective every two hrs for a total of 72 hrs (Camera ...
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bioRxiv - Neuroscience 2023Quote: ... The supernatant was concentrated by centrifugation for 1 hour at 3220 g at 4°C in a 5kDa Cutoff Vivaspin tube (Sartorius, Cat # VS2012). ACM was aliquoted into low protein binding tubes (∼100ul per aliquot) ...
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bioRxiv - Plant Biology 2019Quote: ... These filtrates were ultra-filtered at 4°C through 20-ml Vivaspin micro-concentrators (10,000 molecular weight cut off, Sartorius Stedim, Biotech GmbH, Germany). Retained protein suspensions were used for protein content and enzyme assays ...
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bioRxiv - Biochemistry 2022Quote: ... The dialyzed 2C protein was centrifuged at 75,000 x g at 4 °C for 30 min to isolate soluble protein and concentrated using a Vivaspin Turbo 15 (Sartorius, 10 kDa MWCO) to 2 – 5 mL for preparative gel filtration ...
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bioRxiv - Genomics 2024Quote: The human T lymphoblast Sup T1 cell line was cultured at 37°C under a 95% air and 5% CO2 atmosphere in Roswell Park Memorial Institute medium (RPMI 1640 medium; Sartorius #01-100-1A) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Systems Biology 2023Quote: ... Cells were washed once with warm (37°C) PBS and then lifted from wells using Trypsin EDTA solution B (Sartorius, Cat# 03-052-1B) (5mins ...
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bioRxiv - Bioengineering 2023Quote: ... Batch reactors were incubated for 10 days at a mesophilic temperature of 35°C in a Certomat® BS1 incubator (Sartorius Stedim Biotech, Germany) under continuous agitation (145 rpm) ...
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bioRxiv - Plant Biology 2024Quote: ... The samples were oven dried at 103 °C for 96 h and dry weight (DW, kg) measured (BP110S, Sartorius, Goettingen, Germany; 0.1 mg accuracy). The dry volume (DV ...