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Citations for Sartorius :
201 - 250 of 308 citations for Thioredoxin Domain Containing Protein 3 TXNDC3 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2023Quote: ... Four images per well were acquired at 10x magnification in the green channel (200 ms exposure) and the red channel (600 ms exposure) every 2 to 3 hrs using an Incucyte (Sartorius). A cytotoxicity index was calculated by dividing total green fluorescence intensity per mm2 at every time point by the intensity per mm2 at the first time point ...
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bioRxiv - Synthetic Biology 2023Quote: ... One image per well was acquired at 4x magnification in the green channel (100 ms exposure) and the red channel (600 ms exposure) every 2 to 3 hr using an Incucyte (Sartorius).
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bioRxiv - Biophysics 2023Quote: ... or PPIase) were buffer-exchanged into an NMR buffer using either 5 or 3 kDa MWCO centrifugal concentrators (Vivaspin, Sartorius). The NMR buffer contained 10 mM d4-imidazole at pH 6.6 ...
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bioRxiv - Immunology 2024Quote: ... Plates were imaged every 4-6 hours for 3-7 days using the IncuCyte® Live-Cell Analysis System (Sartorius). 5 images per well at 10x zoom were collected at each time point ...
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bioRxiv - Immunology 2023Quote: ... Antibodies were captured on anti-human Fc AHC2 tips (Sartorius). Data capture was performed using Octet Data Acquisition software version 9.0 (ForteBio) ...
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bioRxiv - Biophysics 2019Quote: ... The protein was concentrated to 0.5 ml with a 100-kDa-cutoff concentrator (Vivaspin 20, Sartorius), and further purified by SEC as above.
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bioRxiv - Plant Biology 2021Quote: ... and concentrated to the indicated total protein concentration (Vivaspin 20, 30 000 MW cut-off, Sartorius). For depletion assay ...
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bioRxiv - Cell Biology 2022Quote: ... Protein was concentrated from the media using VivaSpin Turbo 4 (10000 MWCO) concentrator tubes (Sartorius, VS04T01). Protein samples were run on 4-15% Mini protean TGX stain-free gels (Bio-Rad ...
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bioRxiv - Molecular Biology 2021Quote: ... loaded onto Vivaspin protein concentrator columns with a molecular weight cut-off of 30 kDa (Sartorius) and centrifuged at 4,000 x g at 4 °C until a concentration factor of 20 was achieved ...
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bioRxiv - Plant Biology 2021Quote: ... Proteins eluting from the column were concentrated by ultrafiltration on Vivaspin 20 and 2 columns (Sartorius) with a 5 kDa molecular weight cut-off ...
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bioRxiv - Molecular Biology 2021Quote: ... The proteins were concentrated with Vivaspin 20-mL centrifugal devices with 5,000 or 10,000 MWCO (Sartorius) and buffer-exchanged into NMR buffer over PD-10 gel-filtration columns (GE-healthcare).
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bioRxiv - Biochemistry 2022Quote: ... Protein was pooled and concentrated with a Vivaspin 30 kDa molecular weight cut-off centricon (Sartorius) and loaded onto a buffer A equilibrated S6 Increase 3.2/300 (Cytiva ...
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bioRxiv - Microbiology 2023Quote: ... The tag-free RnpM protein was collected and concentrated using a Vivaspin turbo 15 (Sartorius, Germany).
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bioRxiv - Molecular Biology 2023Quote: ... followed by protein concentration using vivaspin centrifugal concentrators columns (MWCO 3.0 kD, Sartorius GmbH, Göttingen, Germany). Protein contents were analyzed according to Bradford using Rotiquant (Roth ...
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bioRxiv - Biochemistry 2023Quote: ... C-terminally biotinylated SKP1-FBXO22 fusion proteins (WT or C326A) were immobilized onto SA biosensors (Sartorius) to a response level of 1 nm ...
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bioRxiv - Microbiology 2023Quote: ... MTSL-labelled protein was concentrated (Vivaspin® 500 centrifugal filter, 10 kDa MWCO PES membrane, Sartorius). Labelling efficiencies for each Csx23 mutant were assessed by continuous wave (CW ...
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bioRxiv - Cancer Biology 2022Quote: ... tumor cells transfected with plasmids containing eGFP were treated and co-cultured as described above in the IncuCyte® SC5 Live-Cell Analysis system (Sartorius). Plates were scanned with a 10x objective using phase contrast channel as well as the green fluorescent channel for 42 h every 6 h ...
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bioRxiv - Cancer Biology 2022Quote: 700 cancer and 1400 stellate cells were seeded in culture medium containing 1% FBS into the apical compartment of an Incucyte Clearview 96-well plate (Sartorius, 4582) at a 1:2 ratio ...
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bioRxiv - Microbiology 2021Quote: ... using a centrifugation tube with an insert containing a molecular weight cut-off filter (5 kDa, Sartorius Stedim Biotech, Goettingen, Germany). Each HMW fraction was purified by flushing with 20 ml of water ...
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bioRxiv - Microbiology 2021Quote: ... Fractions of IMAC eluate containing Pdr5 were pooled and concentrated using a Vivaspin 6 centrifugal concentrator unit (Sartorius AG, Göttingen, Germany) with a molecular weight cut-off (MWCO ...
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bioRxiv - Immunology 2021Quote: ... 7 L of Sf9 cell culture at a density of 2 × 106 cells/mL were infected with the baculovirus at a MOI of 3 using a Biostat B plus bioreactor (Sartorius, Germany). The following conditions were maintained during the culture period ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were plated at 20,000 cells/ml in Geltrex-coated 96-well plates and treated with different doses of inhibitor in the presence of 1:1000 IncuCyte® Caspase-3/7 Dye for Apoptosis (Sartorius). Doubling times were calculated by determining cell counts over multiple days with the IncuCyte Live Cell Analysis System cell-by-cell module (Sartorius).
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bioRxiv - Cancer Biology 2023Quote: 2,000 cells per well were plated in 96-well plates and treated with the indicated dose of drugs or caspase-3/7 dye for apoptosis (Sartorius, #4440). Cell proliferation and apoptosis were monitored by a microscope gantry that was connected to a network external controller hard drive that gathered and processed image data ...
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bioRxiv - Cancer Biology 2023Quote: Analysis of apoptosis using fluorescence live cell microscopy Cells were stained with Incucyte® Caspase-3/7 Dye for Apoptosis (Sartorius) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... were used to collect air on 37 mm sterile gelatin filters (3 µm pore size; Sartorius, Cat. No. 12602-37-ALK) containing a backing filter to ensure the gelatin filter remained intact during sampling ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Cells were incubated for 3 hours with a red viability probe (1/3000, Incucyte® Nuclight Rapid Red Dye - Sartorius, Germany) incorporated by the nuclei of living cells ...
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bioRxiv - Biochemistry 2020Quote: ... The peak fractions containing ANT(9) were pooled and concentrated to 6 mg/ml using a Vivaspin 10 kDa cut-off concentrator (Sartorius AG, Germany).
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bioRxiv - Microbiology 2020Quote: ... Obtained fractions were analyzed by SDS PAGE in reducing conditions and fractions containing proKLK13 or proKLK14 were concentrated with Vivaspin® 2 (Sartorius, Germany) and further purified using size exclusion chromatography (Superdex s75 pg ...
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bioRxiv - Molecular Biology 2022Quote: ... and fractions containing complete nanodiscs (Fig. S1) pooled and concentrated using a Vivaspin 500 PES 50 kDa cut off spin filter (Sartorius, Cat#VS0131).
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bioRxiv - Microbiology 2020Quote: ... The purified protein was buffer exchanged into PBS using a Vivacon 500 concentrator (Cat: VS0122, Sartorius Stedim). For the generation of human ACE2-hFc and SARS-CoV-2 RBD-mFc recombinant proteins ...
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bioRxiv - Immunology 2021Quote: Biolayer interferometry was performed using the Octet Red96 with Protein A (ProA) biosensors (Sartorius ForteBio, Fremont, CA), which were hydrated for 15 min in kinetics buffer prior to each run ...
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bioRxiv - Microbiology 2021Quote: ... the purified proteins were run on Superdex S200 size-exclusion column and concentrated with Vivaspin devices (Sartorius).
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bioRxiv - Biochemistry 2022Quote: ... Buffer exchange and protein sample concentration were performed using 10 kDa MWCO Vivaspin Turbo centricons (Sartorius, Germany). After IMAC ...
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bioRxiv - Molecular Biology 2021Quote: Biolayer interferometry was performed using the Octet Red96 with Protein A (ProA) biosensors (Sartorius ForteBio, Fremont, CA), which were hydrated for 15 min in kinetics buffer prior to each run ...
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bioRxiv - Biophysics 2022Quote: ... Dialyzed protein was then concentrated with 10 kDa MWCO Vivaspin centrifugal concentrators (3,500 xg, 4°C) (Sartorius). Protein concentrations were determined by means of fluorescence using an sfGFP calibration curve ...
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bioRxiv - Bioengineering 2022Quote: Biolayer interferometry was performed using the Octet Red96 with Protein A (ProA) biosensors (Sartorius ForteBio, Fremont, CA), which were hydrated for 15 min in kinetics buffer prior to each run ...
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bioRxiv - Immunology 2022Quote: The Octet quantification assay for protein expression level was performed on an Octet 96 Red system (Sartorius). Harvested cell media were centrifuged and cell supernatants were prepared in a 96-well plate with S-2P or S-6P standards diluted in media from 20 μg/mL to 0.3125 μg/mL ...
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bioRxiv - Cell Biology 2023Quote: ... protein was eluted and concentrated to 500 μl using a Vivaspin6 10,000 MVCO centrifugal concentrator (Sartorius, Germany). The concentrated sample was subjected to size exclusion chromatography using an Äkta FPLC UPC-900 liquid chromatography system (GE Healthcare ...
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bioRxiv - Biochemistry 2023Quote: ... The mixture of peptides was separated from large proteins by centrifugation on Vivaspin 500 concentrators (Sartorius, Germany) with a PES membrane resistant to high concentrations of salts and detergents (10 kDa molecular weight cut-off) ...
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bioRxiv - Microbiology 2024Quote: ... 10 % glycerol) and proteins were concentrated using vivaspin 6 columns with a 5000 Da cut off (Sartorius). To remove the His-tag ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... as determined by weighing the e-liquid-containing cartridge before and after the carryover experiment using an analytical balance (Sartorius Practum, Göttingen, Germany). E-liquids for carryover experiments were chosen based on type of synthetic coolant present (WS-3 ...
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bioRxiv - Microbiology 2023Quote: ... cells were pelleted by centrifugation for 40 minutes at 3000 g and the phage-containing supernatant was filtered twice (Sartorius MinisartTM 0.2 µm) to obtain a phage stock ...
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bioRxiv - Molecular Biology 2021Quote: ... the clarified supernatant was loaded onto Vivaspin protein concentrator columns (molecular weight cut-off of 30 kDa; Sartorius) and centrifuged at 4,000 x g at 4 °C until the supernatant was 100-fold concentrated ...
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bioRxiv - Neuroscience 2022Quote: ... Protein was filtered with a 0.22 µm filter and concentrated using Vivaspin 15R 10K centrifugal filters (VS15RH01, Sartorius). Protein was then loaded onto a Superdex 200 column and 2 mL fractions were collected ...
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bioRxiv - Biochemistry 2022Quote: ... protein solutions were concentrated to about 12 mg ml-1 using a centricon centrifugal filter unit (Sartorius Stedim). The protein concentration was estimated by measuring the absorbance at 280 nm.
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bioRxiv - Microbiology 2020Quote: ... Eluted proteins were concentrated using an ultrafiltration membrane with a 10 kDa molecular weight cutoff (Vivaspin 6, Sartorius). To reconstitute the BAM-DolP complex in vitro ...
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bioRxiv - Microbiology 2021Quote: ... CV30 or CR3022 IgG at 10ug/mL was loaded onto pre-hydrated Protein A biosensors (Pall FortéBio/Sartorius) for 150 s followed by a 60 s baseline ...
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bioRxiv - Synthetic Biology 2021Quote: ... and 30 μg of total protein was loaded into 30-kDa-cutoff Vivacon 500 ultrafiltration spin columns (Sartorius). Samples were digested for 4 h on a filter with 1:50 Lys-C (Wako ...
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bioRxiv - Microbiology 2023Quote: ... Purified protein fractions were dialyzed against 1X PBS (pH 7.4) using the Vivaspin 10 kDa centrifuge system (Sartorius). Protein quantification was performed in a spectrophotometer at 280 nm and the assays were performed with the enzyme at a concentration of 5.88 μM.
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bioRxiv - Cell Biology 2023Quote: ... Cleaved protein was eluted in fresh cleavage buffer and concentrated to 0.5 mL using a centrifugal concentrator (Sartorius). Protein concentration and quality was determined by NanoDrop® ND-1000 spectrophotometer (Labtech ...