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Citations for Sartorius :
251 - 285 of 285 citations for Tetratricopeptide Repeat Protein 5 TTC5 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: The perfusion cell culture experiments were conducted in a 5 L glass bioreactor using a Biostat B-DCU controller (Sartorius, Göttingen, Germany) with a working volume of 2 L ...
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bioRxiv - Molecular Biology 2023Quote: ... The peak fractions were concentrated to 5-10 mg/ml with a 30-KDa cutoff Vivaspin Turbo 15 (Sartorius AG, Göttingen, Germany), flash-frozen in liquid nitrogen ...
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bioRxiv - Bioengineering 2024Quote: ... The plate was centrifuged for 5 minutes at 100g and then placed in the IncuCyte S3 Live-Cell Analysis System (Sartorius, G_ttingen, Germany) and stored at 37°C ...
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bioRxiv - Immunology 2022Quote: BLI experiments on the antagonistic antibodies were performed using an Octet Red 96 machine (Sartorius) in kinetics buffer (PBS ...
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bioRxiv - Microbiology 2019Quote: ... adapted by us to the use of 100 µg protein for digestion with sequencing-grade trypsin in 50:1 ratios in Vivacon 10k filters (Sartorius AG, Germany) (28) ...
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bioRxiv - Biochemistry 2020Quote: ... bengalensis protein hydrolysates (BBPH) were subjected to centrifugal ultrafiltration using 3kDa cut-off membrane filtration unit (Vivaspin20, VS2091, Sartorius AG, Goettingen, Germany) to separate the low molecular peptide fraction (< 3kDa) ...
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bioRxiv - Plant Biology 2023Quote: The biolayer interferometry was performed as previously described (Xie et al., 2022) using Octet® R2 Protein Analysis System (Sartorius, Figure 1) or Gator-plus (Gator Bio ...
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bioRxiv - Plant Biology 2023Quote: ... Finally, protein concentration and buffer exchange (20 mM Tris-HCl, pH 7.5) was performed using Vivaspin 30K columns (Sartorius, Germany, Göttingen, Germany).
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bioRxiv - Biophysics 2023Quote: ... proteins were exchanged to labelling buffer (20 mM HEPES, 50 mM NaCl, pH 7.5) using VivaSpin 6 concentrator (Sartorius AG, Gottingen, Germany).
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bioRxiv - Biochemistry 2020Quote: ... and the filtrate concentrated using a ultrafiltration apparatus with a 5 kDa molecular mass cut-off (Vivaflow® 200 cassette, Sartorius Stedim Biotech). The concentrate was separated by reversed phase (RP-HPLC ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Samples weighing 5-15mg were dispensed in standard aluminum pans (DSC consumables incorporated, Minnesota, USA) using a microbalance (Sartorius 3.6P microbalance, Göttingen, Germany) and sealed using standard aluminum lids ...
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bioRxiv - Genomics 2024Quote: The human T lymphoblast Sup T1 cell line was cultured at 37°C under a 95% air and 5% CO2 atmosphere in Roswell Park Memorial Institute medium (RPMI 1640 medium; Sartorius #01-100-1A) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Bioengineering 2022Quote: ... and HT29 cells were transfected to express the nuclear-restricted mKate2 red fluorescent protein using the IncuCyte® NucLight Lentivirus Reagents (Sartorius, Cat: 4476) and following the manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: ... Protein production at pilot scale for nonclinical trial material was archived in 500L Bioreactor (BIOSTAT STR®500, Sartorius Stedim Biotech, Göttingen, Germany) using a fed-batch process using PowerCHO-2 medium as basal medium ...
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bioRxiv - Biochemistry 2022Quote: ... The dialyzed 2C protein was centrifuged at 75,000 x g at 4 °C for 30 min to isolate soluble protein and concentrated using a Vivaspin Turbo 15 (Sartorius, 10 kDa MWCO) to 2 – 5 mL for preparative gel filtration ...
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bioRxiv - Microbiology 2023Quote: ... Fc-tagged RBD proteins (20 μg/mL) were captured on the protein A biosensor followed by 30 s of baseline run in 1x kinetics buffer (Sartorius Cat#: 18-1105). The biosensors were blocked with a control Fc protein (Jackson ImmunoResearch Cat# ...
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bioRxiv - Immunology 2022Quote: The binding affinity of obtained antibodies to RBD was examined by the Blitz system (Sartorius Japan) using protein A-coated biosensors ...
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bioRxiv - Synthetic Biology 2022Quote: The binding kinetics of antibodies to RBD was measured by BLI on an Octet-R2 (Sartorius). The his-tagged RBD variants were loaded onto Ni-NTA biosensors from 10 ug\ml solution in kinetic buffer (PBS ...
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bioRxiv - Immunology 2024Quote: ... Monoclonal Moo1 and Moo2 antibodies were immobilized on anti-human IgG Fc Capture (AHC) biosensors (Sartorius) to capture monoclonal antibodies at a concentration of 50 µg/mL for 5 min and washed with octet buffer ...
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bioRxiv - Genomics 2021Quote: ... Each diluted blood sample was then filtered using a 5 μm surfactant-free cellulose acetate syringe filter (Minisart NML® syringe filter, Sartorius reference number: 17594K) connected to a 10 mL syringe (Figure 1) ...
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bioRxiv - Biophysics 2020Quote: ... The flow-through of the column was concentrated (Vivaspin®, mwco 10 or 30 kDa for single SBD or tandem mutants, Sartorius; ~ 5 mg/ml), dialyzed in buffer A supplemented with 50 % glycerol ...
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bioRxiv - Molecular Biology 2020Quote: ... were incubated with 2 mM PKH67 for 5 min and then washed five times using a 100 kDa filter (Sartorius, VIVACON 500 100K VN01H41) to remove excess dye ...
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bioRxiv - Evolutionary Biology 2019Quote: ... The fractions containing MGE protein were pooled and concentrated to 1 mL by using a centrifugal concentrator (Sartorius, 10 kDa MWCO, Vivaspin turbo 15). The recombinant proteins were further purified by ion exchange and gel filtration chromatography with ÄKTAprime plus system ...
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bioRxiv - Evolutionary Biology 2019Quote: ... The fractions containing MGE protein were pooled and concentrated to 1 mL by using a centrifugal concentrator (Sartorius, 10 kDa MWCO, Vivaspin turbo 15). For gel filtration ...
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bioRxiv - Immunology 2020Quote: ... The antibodies were buffer-exchanged into PBS and concentrated using 10,000 MWCO Vivaspin centrifugal spin columns (Sartorius).
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bioRxiv - Immunology 2024Quote: ... Supernatants were quantitated for antibody concentrations by bio-layer interferometry (BLI) on an Octet Red 96 (Sartorius) using protein A biosensors and a human IgG1 isotype control antibody as a standard ...
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bioRxiv - Immunology 2024Quote: ... The antibodies were buffer-exchanged into PBS and concentrated using 10,000 MWCO Vivaspin centrifugal spin columns (Sartorius).
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bioRxiv - Bioengineering 2023Quote: ... Antibody CSL362 hIgG1 (captured ligand) was captured by Anti-Human Fc capture biosensor (AHC) (Sartorius, PN: 18-5060) for 300s at 0.5µg/mL ...
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bioRxiv - Immunology 2024Quote: ... Antibodies were diluted to a final concentration of 20 ug/mL before being immobilized on ProteinA tips (Sartorius) for 600 s ...
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bioRxiv - Microbiology 2020Quote: ... every 5 min for up to 20 h or until a quasi-equilibrium was reached using a micro-balance (Sartorius MSE3.6P-000-DM, readability 0.0010 mg). The chamber of the micro-balance was half-opened to keep air circulating with the environmental chamber ...
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bioRxiv - Immunology 2021Quote: Affinity measurements for VHH-huFc antibodies were performed using biolayer interferometry (BLI) using an Octet 384 Red system (Sartorius). Measurements were conducted in 10 mM phosphate (pH 7.4) ...
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bioRxiv - Immunology 2023Quote: The binding affinity of recombinant antibodies to antigens was estimated by bio-layer interferometry (Octet® QK, ForteBio/Sartorius). Antibodies were loaded at 10-20 nM onto anti-human IgG Fc capture or anti-Mouse IgG Fc capture biosensors ...
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bioRxiv - Immunology 2024Quote: Analysis of serum antibodies binding to PNP scaffolds by biolayer interferometry (BLI) was performed on an Octet R4 (Sartorius) at 30 °C in kinetics buffer (PBS ...
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bioRxiv - Bioengineering 2022Quote: ... The five fermentation experiments to produce a monoclonal antibody were all conducted in controlled automated miniaturized bioreactor (ambr250) systems (Sartorius AG, Germany) for 14 days ...
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bioRxiv - Synthetic Biology 2021Quote: ... The required amount of antibody was diluted in PBS (5ml) and applied to a Vivaspin 6 concentrator (Sartorius; VS0601; MWCO: 10kDa; 5ml volume). After centrifugation (4000 x g ...