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Citations for Sartorius :
1 - 50 of 68 citations for Saporin Goat Polyclonal affinity purified Alexa488 labeled since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2020Quote: ... The AAV2/8 was purified by affinity chromatography and concentrated using ultrafiltration columns (Sartorius Stedim Biotech, Goettingen, Germany), washed in PBS and concentrated to a volume of 100–150 µl ...
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bioRxiv - Cancer Biology 2023Quote: ... and labeled using pHrodo red (Sartorius) at a final concentration of 31.3 ng/mL for 45 minutes at 37°C in pHrodo Labeling Buffer (Sartorius) ...
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bioRxiv - Microbiology 2021Quote: ... The affinity measurement was performed on the ForteBio Octet RED 96 system (Sartorius, Goettingen, Germany). Briefly ...
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bioRxiv - Immunology 2022Quote: The binding affinity of obtained antibodies to RBD was examined by the Blitz system (Sartorius Japan) using protein A-coated biosensors ...
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bioRxiv - Microbiology 2021Quote: ... The affinity measurement was performed on the Forte bio Octet RED 96 system (Sartorius, Goettingen, Germany). Briefly ...
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bioRxiv - Immunology 2022Quote: ... The affinity chromatography eluate was subjected to concentration and diafiltration using TFF (Sartorius Stedim Biotech GmbH). In order to produce the immunoaffinity resin for PF-D-Trimer purification ...
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bioRxiv - Microbiology 2024Quote: ... pooled enzyme samples after affinity chromatography were concentrated using centrifugal concentrators (10 kDa cutoff) (Sartorius, Germany) and applied to size exclusion chromatography (Superdex 200 HiLoad 26/60 prep grade column or Superose 6/100 column (Cytavia ...
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bioRxiv - Molecular Biology 2023Quote: ... The labeled tip was then dipped in 1x binding buffer (Sartorius) for 60 seconds to remove excess ligand and achieve baseline ...
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bioRxiv - Cell Biology 2020Quote: ... purified (Vivapure AdenoPACK 100, Sartorius) and titred using a TCID50 assay ...
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bioRxiv - Neuroscience 2022Quote: The affinity was measured by Bio-Layer Interferometry (BLI) using the Octet qKe (Fortebio/Sartorius GmbH, Göttingen, Germany). For this assay ...
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bioRxiv - Immunology 2023Quote: Affinity measurements for the BTN2A1-BTN3A1 interaction were determined by BLI on an Octet Red 96e instrument (Sartorius). Assays were performed in black 96 well flat-bottom plates at 25°C with agitation (1000 rpm) ...
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bioRxiv - Cancer Biology 2023Quote: ... The binding affinity between HER3-ECD and LRG1 or neuregulins was measured by the Octet RED96 system (Sartorius), at 25 °C in 1X PBS buffer ...
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bioRxiv - Physiology 2023Quote: ... purified using Vivapure AdenoPACK 100 (Sartorius), and titred as described previously (21) ...
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bioRxiv - Developmental Biology 2022Quote: The binding affinities of SPACA6 to IZUMO1 and JUNO were measured by BLI using a single-channel BLItz instrument (FortéBio/Sartorius). Purified JUNO and IZUMO1 in PBS were biotinylated using the EZ link sulfo-NHS-LC-biotinylation kit (Thermo Pierce) ...
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bioRxiv - Immunology 2021Quote: Affinity measurements for VHH-huFc antibodies were performed using biolayer interferometry (BLI) using an Octet 384 Red system (Sartorius). Measurements were conducted in 10 mM phosphate (pH 7.4) ...
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bioRxiv - Immunology 2023Quote: The binding affinity of recombinant antibodies to antigens was estimated by bio-layer interferometry (Octet® QK, ForteBio/Sartorius). Antibodies were loaded at 10-20 nM onto anti-human IgG Fc capture or anti-Mouse IgG Fc capture biosensors ...
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bioRxiv - Biochemistry 2024Quote: Kinetic and steady state binding affinities were measured by BLI as previously described (Bruguera et al., 2022; Mahoney et al., 2022) using Octet RED384 (Sartorius) or GatorPrime (Gator Bio ...
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bioRxiv - Microbiology 2024Quote: ... the fractions obtained by affinity chromatography were pooled and concentrated using conical membrane ultrafiltration tubes (Vivaspin 6 mL 10 kDa MWCO, Sartorius) to 7 mg/mL (2 mL) ...
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bioRxiv - Molecular Biology 2020Quote: ... purified using the Vivapure Adenopack 100 (Sartorius) and titrated by end-point dilution in HEK293 cells.
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bioRxiv - Neuroscience 2024Quote: ... then labeled with the pHrodo Red Cell Labeling Kit for Incucyte (4649; Sartorius, Bohemia, NY) with a final concentration of 125 ng/mL dye following manufacturer’s instructions ...
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bioRxiv - Biochemistry 2020Quote: ... The protein was expressed and purified as previously described.26 The purified protein was concentrated to 10-20 mg/mL using a Vivaspin15R (Sartorius), and purity was estimated to be at least 95% by SDS-PAGE.
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bioRxiv - Biochemistry 2020Quote: Purified proteins were concentrated with VIVASPIN concentrators (Sartorius) and dialyzed against E-Buffer with 1 mM DTT ...
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bioRxiv - Immunology 2022Quote: ... and aged wild-type mice were isolated and labeled using Incucyte Caspase3/7 Red Reagent (Sartorius). Cells were stimulated with 5 μg/mL plate-bound αCD3 (Biolegend ...
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bioRxiv - Microbiology 2021Quote: ... Purified NS1 was then concentrated using Vivaspin columns (Sartorius).
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bioRxiv - Biophysics 2023Quote: ... Purified proteins were concentrated using centrifugal filter devices (Vivaspin, Sartorius). Protein concentration was estimated from its absorbance at 280 nm ...
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bioRxiv - Microbiology 2020Quote: ... The purified Rel was concentrated in a Vivaspin turbo 15 (Sartorius) centrifugal filter device (cut-off 50 kDa) ...
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bioRxiv - Immunology 2021Quote: ... Purified nucleoproteins were concentrated by ultrafiltration using appropriate VivaSpin devices (Sartorius), were snap-frozen in liquid nitrogen in small aliquots and stored at −80°C.
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bioRxiv - Microbiology 2023Quote: ... The purified proteins were concentrated in a Vivaspin turbo 15 (Sartorius) centrifugal filter device (cut-off 5 or 50 kDa) ...
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bioRxiv - Immunology 2022Quote: Splenic CD4+ and CD8+ T-cells from Ldlr-/- and T-AbcdkoLdlr-/- mice were isolated and labeled using Incucyte Caspase3/7 Red Reagent (Sartorius). Cells were cultured in RPMI 1640 medium (Gibco ...
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bioRxiv - Biochemistry 2021Quote: ... Fractions containing purified β2HA were pooled and concentrated on Vivaspin columns (Sartorius).
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bioRxiv - Biophysics 2023Quote: ... Purified nucleocapsid tubes were concentrated using Vivapore solvent absorption chambers (Sartorius, Germany) and subsequently used for further processing ...
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bioRxiv - Biochemistry 2021Quote: ... Purified Spike was concentrated using a 100 kDa cut-off concentrator (Sartorius, GE) and loaded onto a HiLoad 16/60 superdex 200 pre-equilibrated with 20 mM Hepes ...
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bioRxiv - Microbiology 2020Quote: ... concentrated and purified using Vivaspin 20 ultrafiltration units (300kDa MW cut-off, Sartorius), and stored at −80°C.
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bioRxiv - Immunology 2020Quote: ... Purified viral particles were obtained by Ion Exchange (VivaPure® AdenoPACKTM 20, Sartorius), and the DNA extracted and subjected to Next Generation Sequencing ...
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bioRxiv - Microbiology 2020Quote: ... The purified antibodies were buffer exchanged to PBS using 100 kDa VivaSpin20 columns (Sartorius). The IgG concentration was determined on the NanoDrop 2000 and the antibodies were stored at 4°C until further analyses.
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bioRxiv - Neuroscience 2020Quote: ... Water was purified using an Arium 611 Pro VF purification system (Sartorius, Göttingen, Germany) to laboratory-graded water (18.2 MΩ × cm).
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bioRxiv - Microbiology 2019Quote: ... Purified schizonts were ruptured with a 1.2 μm Ministart syringe filter (Sartorius Stedim Biotech) to release individual merozoites and 25 μl merozoite solution was added to blood obtained from mice of interest (10 ul at 100,000 cells per μl ...
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bioRxiv - Bioengineering 2022Quote: ... 25ng/ul of purified mAbs were captured on a AHC biosensor (Sartorius, 18-5060). The baseline was recorded for 60s in a running buffer (PBS ...
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bioRxiv - Cell Biology 2019Quote: ... Virus was amplified and purified using AdenoPACK 20 Adenovirus (Ad5) purification & concentration kit (Sartorius).
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bioRxiv - Plant Biology 2022Quote: Deionized water was purified with the Arium® purification system (Sartorius AG, Goettingen, Germany). Methanol was of LC-MS grade ...
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bioRxiv - Biochemistry 2021Quote: ... Purified enzyme was concentrated (30 kDa cutoff Amicon Stirred Cell, Millipore or VivaSpin4 columns, Sartorius). Protein concentration was determined spectrophotometrically at 280 nm (μCuvette ...
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bioRxiv - Microbiology 2022Quote: ... The aliquots containing the purified His-ChuP were concentrated using a VivaSpin 6 column (Sartorius), and desalted by gel filtration in PD-10 column (GE Healthcare ...
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bioRxiv - Microbiology 2020Quote: ... Samples containing purified protein were concentrated using Vivaspin® 6 5,000 MWCO columns (Sartorius Stedim). Protein was buffer exchanged using membrane filtration into 50 mM NaP ...
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bioRxiv - Immunology 2022Quote: ... Purified protein was buffer exchanged into PBS and concentrated using a Vivaspin concentrator (Sartorius-Stedim) to 3-5 mg/mL and flash-frozen with liquid nitrogen and stored at −80 °C.
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... was purified using centrifugal filtration (cut-off 30 kDa, Vivaspin 500, Sartorius AG, Goettingen, Germany) eight times for 2 min at 15’000 × g at 4°C ...
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bioRxiv - Biochemistry 2022Quote: ... Purified His6-MBP-tagged proteins were then concentrated with a 30,000 MWCO centrifugal concentrator (Sartorius) and buffer exchanged into 20 mM HEPES pH 7.5 and 50 mM NaCl for crystallization ...
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bioRxiv - Cell Biology 2023Quote: Low molecular weight alginate (L3, Kimica) was purified using a tangential flow filtration system (Sartorius) and subsequently coupled with GGGRGDSP peptide (Biomatik ...
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bioRxiv - Microbiology 2021Quote: ... supernatant was harvested and virus particles were purified from cytokines and concentrated using Vivaspin 20 (Sartorius, filtration units with a size exclusion of 100 kDa ...
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bioRxiv - Microbiology 2020Quote: ... The purified protein was buffer exchanged into PBS using a Vivacon 500 concentrator (Cat: VS0122, Sartorius Stedim). For the generation of human ACE2-hFc and SARS-CoV-2 RBD-mFc recombinant proteins ...
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bioRxiv - Microbiology 2021Quote: ... the purified proteins were run on Superdex S200 size-exclusion column and concentrated with Vivaspin devices (Sartorius).