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Citations for Sartorius :
1 - 50 of 189 citations for Recombinant Mouse Scavenger Receptor Class B Member 1 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2022Quote: ... His-tagged antigens using Anti-Penta-HIS biosensors (Sartorius/ForteBio). Antigen was loaded to a threshold of 1 nm shift ...
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bioRxiv - Biophysics 2024Quote: ... His-tagged dROS1 protein (construct 2-3) were loaded onto penta-His sensors (Sartorius) to a response unit of about 0.5 nm followed by quenching in 10 ng/mL biocytin (Sigma-Aldrich ...
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bioRxiv - Immunology 2021Quote: ... His-tagged ACE2 was immobilized onto Ni-NTA coated biosensors (Sartorius) before association of increasing concentrations of each RBD for 120 seconds followed by dissociation using HBS pH 7.4 for 360 seconds ...
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bioRxiv - Immunology 2021Quote: ... recombinant SARS-CoV-2 S2-His-tag at 10 µg/mL was loaded on Anti-Penta-HIS (HIS1K) biosensors (Sartorius Corporation) for 1 min ...
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bioRxiv - Immunology 2021Quote: His-tagged FcαRI (Sinobiological, Cat: 10414-H08H) were immobilized via Ni-NTA biosensor (Sartorius, Cat: 18-5101) and subject to free floating IgA variants to obtained ka ...
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bioRxiv - Immunology 2021Quote: ... S2-His-tag was immobilized on Anti-Penta-His (HIS1K) biosensors (Sartorius Corporation), and a concentration series of S2 binding mAb Fab at 200 ...
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bioRxiv - Immunology 2021Quote: ... Anti-penta-HIS biosensors (Sartorius) were immersed in kinetics buffer (PBS + 0.5% BSA + 0.05% Tween-20 ...
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bioRxiv - Immunology 2022Quote: ... Anti-penta-HIS biosensors (Sartorius) were immersed in kinetics buffer (PBS + 0.5% BSA + 0.05% Tween-20 ...
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bioRxiv - Biochemistry 2022Quote: ... or HIS 1K biosensors (Sartorius/ForteBio) (CrossMAb ...
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bioRxiv - Biochemistry 2022Quote: Anti-His-tag (HIS1K) biosensors (Sartorius) were incubated in BLI buffer (0.002% v/v Tween-20 in 1× PBS ...
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bioRxiv - Systems Biology 2024Quote: ... negative and positive datasets respectively were imported into soft independent modelling of class analogy (SIMCA) version 17.0 software (Sartorius, South Africa) for chemometrics modelling ...
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bioRxiv - Microbiology 2023Quote: ... 2 L or 5 L scale (Biostat B-plus and B-DCU units, Sartorius Stedium Biotech) as previously described [PMID ...
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bioRxiv - Immunology 2022Quote: ... His-tagged SARS-CoV-2 RBD was prepared at 8 μg ml−1 and loaded on prehydrated anti-penta-HIS biosensors (Sartorius) for 3 min ...
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bioRxiv - Microbiology 2020Quote: A BIOSTAT® B bioreactor (Sartorius Stedim) with a 1.5-L capacity UniVessel® glass vessel was used for growth of 800-mL cultures at 37°C ...
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bioRxiv - Synthetic Biology 2022Quote: ... The seed culture from each flask was transferred to a 1-L bioreactor (Biostat B-DCU, Sartorius, Germany) with 0.7 L fermentation medium ...
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bioRxiv - Bioengineering 2022Quote: ... and was captured by anti-Penta-His (HIS1K) sensors (Sartorius, 18-5120). The primary antibody was diluted to 150nM with a running buffer in wells ...
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bioRxiv - Zoology 2022Quote: ... and 500 µL 10X Amphotericin B (AmB) Solution (Sartorius) for 3 times ...
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bioRxiv - Immunology 2021Quote: ... IncuCyte® Annexin V Green Reagent was added directly in the medium at the dilution 1:200 and B cells were imaged every hour for 3.5 days using IncuCyte® Live-Cell Analysis System (Sartorius).
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bioRxiv - Cell Biology 2022Quote: ... or mCherry-tagged histone 2B (mCherry-H2B) were performed using an Incucyte SX5 (Sartorius Imaging). Images were taken once every 60 minutes to acquire videos of cells monitored over a 72-hour period.
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bioRxiv - Biochemistry 2022Quote: ... Purified His6-MBP-tagged proteins were then concentrated with a 30,000 MWCO centrifugal concentrator (Sartorius) and buffer exchanged into 20 mM HEPES pH 7.5 and 50 mM NaCl for crystallization ...
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bioRxiv - Biochemistry 2021Quote: ... 12xHis-tagged EPCRWT or EPCRY154A was immobilized on the surface of NiNTA pre-coated biosensors (Sartorius), at a concentration of 100 µg/mL ...
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bioRxiv - Microbiology 2019Quote: The experimental platform is an improved version of a previous on-line flow cytometry platform [28][12] and actually comprises three modules (Figure 1): (a) a conventional culture device (Biostat B-Twin, Sartorius, 2 L), a physical interface for sampling and dilution comprising peristaltic pumps and a mixing chamber (c ...
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bioRxiv - Physiology 2019Quote: ... were inoculated with 2 mL of stock culture and grown at 30°C and at 200 rpm for 8 h in a B Braun Certomat BS-1 incubator (Sartorius, Melsungen, Germany). Bioreactor batch cultures were started by transferring 400 mL of preculture to a bioreactor containing 4.6 L of medium ...
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bioRxiv - Microbiology 2022Quote: ... The aliquots containing the purified His-ChuP were concentrated using a VivaSpin 6 column (Sartorius), and desalted by gel filtration in PD-10 column (GE Healthcare ...
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bioRxiv - Microbiology 2023Quote: ... The eluted proteins were concentrated with a centrifugal device (MWCO 30 kDa for soluble EFNB2 proteins and 50 kDa for soluble Eph receptor proteins; Sartorius) before being separated on a Superdex 200 Increase 10/300 GL (Cytiva Life Sciences ...
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bioRxiv - Immunology 2024Quote: ... 222 nM of 6xHis-tagged BG505 SOSIP was immobilized with nickel-charges tris-NTA (Ni-NTA) biosensors (Sartorius) for 10 min and transferred to PBS for 1 min to wash off unbound SOSIP ...
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bioRxiv - Microbiology 2021Quote: ... putida KT2440 was cultivated in a stirred bioreactor (Biostat B-Twin, Sartorius) in continuous mode with glucose feed pulses (0.5 g L-1 per pulse ...
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bioRxiv - Microbiology 2023Quote: ... putida KT2440 was grown in a stirred bioreactor (Biostat B-Twin, Sartorius) with a total volume of 2 L and a working volume of 1 L ...
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bioRxiv - Immunology 2022Quote: KD measurements of Fc-tagged Human FcμR (Cat: 13556-H02H, SinoBiological) immobilised on Anti-Human IgG Fc (AHC) (Cat: 18-5060, Sartorius) biosensors were performed on the Octet Red96® system with the loading threshold set at 1.0 nm and utilizing the recombinant Pertuzumab and Trastuzumab IgM VH whole antibodies variants in five serial diluted concentrations (12.5 to 200nM ...
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bioRxiv - Immunology 2021Quote: RBD-His-tag at 5 µg/mL was first loaded on Ni-NTA (NTA) biosensors (Sartorius Corporation) for 15 sec and subsequently blocked with 5 µg/mL mAbs or BLI assay buffer for 5 min ...
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bioRxiv - Systems Biology 2022Quote: ... live-cell proliferation and expression of YFP-tagged proteins were tracked for 48 h using an Incucyte S3 device (Sartorius).
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bioRxiv - Cell Biology 2022Quote: ... His-PKR was further concentrated to at least 10 mg/ml using Vivaspin centrifugal concentrator 30 K (Sartorius), snap-frozen in liquid nitrogen and stored at −80 °C.
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bioRxiv - Systems Biology 2021Quote: ... A 2 L bioreactor equipped with a Sartorius BIOSTAT B® fermentation controller (Sartorius Stedim Biotech GmbH ...
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bioRxiv - Systems Biology 2022Quote: Co-culture experiments were performed in lab-scale stirred bioreactors (Biostat B-Twin, Sartorius). The processes were performed with a working volume of 1L at a constant temperature of 30°C ...
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bioRxiv - Cell Biology 2022Quote: ... GCP6-tagged purified γ-TuRC was then subjected to buffer exchange using Vivaspin 500 centrifugal concentrator (10 kDa MWCO, Sartorius VS0102) for a final NaCl concentration of 150 mM in eluate ...
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bioRxiv - Immunology 2021Quote: ... His-tagged SARS-CoV-2 RBD was prepared at 8 μg/ml in Kinetics buffer (PBS 0.01% BSA) and loaded on pre-hydrated anti-penta-HIS biosensors (Sartorius) for 2.5 min ...
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bioRxiv - Biophysics 2024Quote: ... The 50 ml containing 6-His-AdhE was concentrated to a final volume of 10.5 ml using a Vivaspin 20 centrifugal concentrator (Sartorius) with a molecular weight cut-off of 30 kDa and then dialysed against buffer A ...
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bioRxiv - Synthetic Biology 2019Quote: pH-Controlled bioreactor batch fermentations were conducted with a Biostat B+ (Sartorius Stedim, NY, USA) dual 1.5 L fermentation system at a working volume of 1 L M9 hybrid medium ...
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bioRxiv - Microbiology 2021Quote: Cultures in segregostat mode were performed in lab-scale stirred bioreactor (Biostat B-Twin, Sartorius) with 1 L working volume ...
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bioRxiv - Bioengineering 2024Quote: The three-step batch cocultivation was done by using Biostat B Plus bioreactor (Sartorius, Germany) equipped with pH-meter (Hamilton ...
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bioRxiv - Plant Biology 2019Quote: ... The protein without His-Tag was concentrated using a Vivaspin 20 Ultrafiltration Unit (3 kDa molecular weight cutoff, Sartorius, www.sartorius.de). An appropriate volume was applied to a 1 ml loop of a gel filtration column (Superdex75 10/300 GL ...
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bioRxiv - Immunology 2021Quote: ... His-tagged SARS-CoV-2 RBD was prepared at 8 µg/ml and loaded on pre-hydrated anti-penta-HIS biosensors (Sartorius) for 2.5 min ...
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bioRxiv - Biochemistry 2022Quote: The specificity of the binding consisted in the immobilization of 2 μg/mL of purified VHHs on Anti-His coated sensors (His1K, Sartorius) via their His6 tag ...
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bioRxiv - Bioengineering 2021Quote: Bioreactor cultivation was performed as duplicates in 2 L Biostat B-DCU bioreactors (Sartorius AG, Germany) with a working volume of 1.5 L ...
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bioRxiv - Microbiology 2022Quote: ... thermolithotrophicus was continuously grown in a 10 L fermenter (BIOSTAT® B plus, Sartorius, Göttingen, Germany) under diazotrophic conditions with either 10 mM Na2SO3 or Na2SO4 as sulfur source instead of Na2S ...
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bioRxiv - Immunology 2023Quote: ... The GRAd32 S-2P vector was expanded in a 2 L Bioreactor (Biostat B DCU; Sartorius). The titer of virus contained in the bulk cell lysates was quantified by qPCR ...
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bioRxiv - Biochemistry 2022Quote: ... IgGs produced from the scFvs from the above sort were assessed for their competition of binding with one another using anti-Penta HIS (His1K) biosensors (Sartorius/ForteBio). His1K tips were pre-quenched with buffer containing 10nM biotin ...
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bioRxiv - Microbiology 2020Quote: ... For all recombinant proteins,protein-containing fractions were pooled and buffer exchange to PBS via VivaSpin columns (Sartorius) was performed ...
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bioRxiv - Systems Biology 2021Quote: ... faecalis V583 ΔglnA was grown in glucose-limited chemostat cultures in Biostat B Plus benchtop bioreactors (Sartorius) in 750 ml CDM-LAB with a dilution rate of 0.15/h at 37 °C and gassing with 0.05 L/min nitrogen and stirring with 250 rpm ...
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bioRxiv - Immunology 2020Quote: ... Vectors were then expanded up to a production in 2L Bioreactor (Biostat B DCU, Sartorius Stedim Biotech), whereby suspension cells were seeded at 5e5 cells/mL ...