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Citations for Sartorius :
101 - 150 of 278 citations for Recombinant Human ROR1 protein His tagged R PE labeled since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... appropriate fractions (selected by 4-20% gradient SDS-PAGE) were pooled and concentrated by centrifugal filtration (Vivaspin 20, 5,000 MWCO PES, Sartorius) for loading onto a Superdex 200 Increase 10/300 GL (GE Healthcare ...
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bioRxiv - Microbiology 2020Quote: ... Reactions were incubated for 16 h at 37 °C following removal of RiGH26 using a Vivaspin 20 filtration unit (10.000 MWCO PES, Sartorius) and carbohydrate lyophilization on an ALPHA 2-4 LD Plus freeze dryer (Christ ...
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bioRxiv - Neuroscience 2020Quote: ... the iodixanol was removed from the AAV vector containing fraction by 3 rounds of diafiltration using Vivaspin 20 ultrafiltration devices (100’000 MWCO, PES membrane, Sartorius) and 1x phosphate buffered saline (PBS ...
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bioRxiv - Biophysics 2023Quote: ... The eluted protein was then concentrated to the approximate concentration of 10 mg/ml using a Vivaspin 6 3,000 MWCO PES (Sartorius) membrane ultrafiltration tube.
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bioRxiv - Cancer Biology 2023Quote: ... Its binding to LIF was confirmed using recombinant LIF (ACRO Biosystems, LIF-H52H3) on an Octet RED 96e biolayer interferometry instrument (Sartorius).
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bioRxiv - Molecular Biology 2023Quote: Human NIL iPSCs were cultured in Nutristem (Sartorius, #05-100-1A) supplemented with Pen/Strep 0.1% on geltrex-coated plates (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2022Quote: ... Protein A biosensors (Sartorius, #18-5010) were hydrated in BLI assay buffer for 10-20 minutes and loaded onto an Octet Red96 BLI instrument (Pall ...
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bioRxiv - Cell Biology 2023Quote: ... Live-cell imaging was done with a S3 Incucyte (S3/SX1 G/R Optical Module, Sartorius) for red and phase channels ...
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bioRxiv - Systems Biology 2020Quote: 2 mL of collected spent media was loaded in Vivaspin 6™ Centrifugal Concentrator 10,000 MWCO PES (Sartorius AG, Germany) and centrifuged at 4000 RPM for 13 minutes to concentrate samples ...
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bioRxiv - Cell Biology 2023Quote: ... Cell-free supernatants were concentrated to 1 mL by ultrafiltration through a membrane with a molecular weight cut-off of 30 kDa (VivaSpin 20, 30,000 MWCO PES (Sartorius, VS2022)) at 4 °C and 4,000 ˗ g ...
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bioRxiv - Microbiology 2019Quote: ... Residual proteins were then eliminated by filtration on 0.22-µm high-protein-retention Ministart syringe filters (Sartorius Stedim Biotech GmbH ...
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bioRxiv - Immunology 2022Quote: Splenic CD4+ and CD8+ T-cells from Ldlr-/- and T-AbcdkoLdlr-/- mice were isolated and labeled using Incucyte Caspase3/7 Red Reagent (Sartorius). Cells were cultured in RPMI 1640 medium (Gibco ...
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bioRxiv - Biochemistry 2020Quote: ... The clarified supernatant was concentrated by ultrafiltration to approximately 70 ml using one 200 cm2 10 kDa MWCO PES tangential flow filtration cassette (Sartorius, Germany) and the ultrafiltration reservoir (Sartorius) ...
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bioRxiv - Microbiology 2019Quote: The faecal supernatant from the pre-processing was filtered through 0.45 µm Minisart® High Flow PES syringe filter (Cat. No. 16533, Sartorius, Germany) to remove bacteria and other larger particles ...
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bioRxiv - Microbiology 2022Quote: ... supernatants were concentrated using a Vivaflow® 200 crossflow cassette with a 10000 MWCO PES membrane (Sartorius, Göttingen, Germany; Cat.# VF20P0) and loaded onto a 5 mL GE Healthcare HisTrap™ FF Crude prepacked column (Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2023Quote: ... pH 7.0 to remove the salt and undesired low molecular weight (LMW) molecules (<10 kDa) from sample using 0.10 m2 Sartocon slice PES cassette (Sartorius Stedim, Germany) and AKTA flux 6 system (GE Healthcare ...
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bioRxiv - Molecular Biology 2023Quote: ... before collecting the upper 90% of supernatant and washing it three times in 50,000 MWCO centrifugal concentrators (Vivaspin 500, PES; Sartorius, Göttingen, Germany). Efficient removal was verified by MALDI-TOF mass spectrometry ...
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bioRxiv - Immunology 2022Quote: ... the supernatant was applied to 2 kDa centrifugal concentrators (Vivaspin 15 R Hydrosart filter device, Sartorius, Germany) and centrifuged for 1 h at 3000 x g according to the suppliers’ protocol ...
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bioRxiv - Neuroscience 2020Quote: ... using protein concentrator tubes (VIVASPIN 20, Sartorius). Both purified IgG fractions from patient and controls were re-tested on CBAs and the anti-IgLON5 IgG concentration was determined (titer 1:100) ...
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bioRxiv - Cancer Biology 2020Quote: Proteins were transferred to nitrocellulose membrane (Sartorius). The membranes were blocked with 10% BSA (Santa Cruz Biotechnology ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins were concentrated using centrifugal concentrator (Sartorius) and further purified with size exclusion chromatography using a Superdex 200 column (GE Life Sciences) ...
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bioRxiv - Microbiology 2021Quote: ... For virome sequencing they were filtered through a 0.45 μm pore size PES filter (Minisart® High Flow Syringe Filter, Sartorius, Göttingen, Germany). For the metagenome sequencing DNA was extracted using the method of Mortensen et al [42] and the DNA was sequenced ...
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bioRxiv - Microbiology 2022Quote: The purified proteins were concentrated and exchanged to 10 mM sodium citrate buffer (pH 5.0) using Vivaspin® Turbo 4 ultrafiltration units with a 5000 MWCO PES membrane (Sartorius; Cat.# VS04T11). The purity and concentration of each PcaLOOL was assessed by SDS-PAGE and the Pierce™ BCA Protein Assay Kit ...
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bioRxiv - Molecular Biology 2022Quote: ... and fractions containing complete nanodiscs (Fig. S1) pooled and concentrated using a Vivaspin 500 PES 50 kDa cut off spin filter (Sartorius, Cat#VS0131).
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bioRxiv - Cancer Biology 2021Quote: ... Beads detecting cytokines from human were analyzed on an IQue Screener Plus (Sartorius). All assays were analyzed using quintuplicate determinations.
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bioRxiv - Evolutionary Biology 2022Quote: ... IgG was loaded onto Anti-Human Fc Capture (AHC) biosensors (Sartorius, #18-5060). To reduce the avidity effect ...
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bioRxiv - Microbiology 2023Quote: ... The eluted proteins were concentrated with a centrifugal device (MWCO 30 kDa for soluble EFNB2 proteins and 50 kDa for soluble Eph receptor proteins; Sartorius) before being separated on a Superdex 200 Increase 10/300 GL (Cytiva Life Sciences ...
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bioRxiv - Cell Biology 2019Quote: ... Proteins were concentrated using a Vivaspin concentrator (Sartorius) with a half lower MWCO than the size of the protein being purified ...
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bioRxiv - Biochemistry 2020Quote: ... Proteins were concentrated using Vivaspin centrifugal filters (Sartorius) of appropriate cut-offs ...
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bioRxiv - Biochemistry 2020Quote: Purified proteins were concentrated with VIVASPIN concentrators (Sartorius) and dialyzed against E-Buffer with 1 mM DTT ...
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bioRxiv - Biochemistry 2022Quote: ... using a 100kDa MWCO Vivaspin protein concentrator (Sartorius), aliquoted ...
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bioRxiv - Biochemistry 2021Quote: ... Proteins were concentrated using Vivaspin concentrators (Sartorius Stedin) and the sample purity and homogeneity was assessed by SDS-PAGE.
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bioRxiv - Biophysics 2023Quote: ... The supernatant was filtered with a 0.45 μm PES filter and concentrated to approximately 100 mL using a Vivaflow® 200 cross-flow cassette (Sartorius Cat No. VF20P2).
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bioRxiv - Immunology 2023Quote: ... The supernatant was filtered with a 0.45 μm PES filter and concentrated to approximately 100 mL using a Vivaflow® 200 cross-flow cassette (Sartorius Cat No. VF20P2).
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bioRxiv - Cancer Biology 2021Quote: ... the 10,000xg supernatant was concentrated down to 1.0 ml with a 10 kDa molecular weight cut-off Vivaflow 50 R tangential flow (TFF) device (Sartorius) and a 10 kDa Amicon Ultra-15 centrifugal filter unit (Merck Millipore ...
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bioRxiv - Molecular Biology 2022Quote: Human primary keratinocytes were plated in Essen ImageLock 96-well plates (Sartorius, Göttingen, Germany) at 15,000 cells per well for migration assay and in 12-well plates at 20,000 cells per well for cell growth assay ...
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bioRxiv - Biophysics 2021Quote: ... Eluted protein was concentrated using centrifugal concentrators (VivaSpin, Sartorius) and then assessed for successful labeling by measuring fluorescence using the following settings and the monochromator in the BioTek Synergy Neo2 plate reader ...
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bioRxiv - Cell Biology 2023Quote: ... 2mM DTT and proteins concentrated via centrifugation (Vivaspin, Sartorius). Recombinant kinase was added to 10µl of 2X kinase buffer [40mM Hepes (pH 7.5) ...
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bioRxiv - Cancer Biology 2023Quote: The human plasma samples were filtered (Minisart NML syringe filters, pore size: 0.8 µm; Sartorius) and diluted with 1x Phosphate-Buffered Saline without calcium and magnesium buffer (Gibco ...
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bioRxiv - Immunology 2023Quote: ... BLI binding experiments were performed with Anti Human IgG Fc (AHC) biosensors (Sartorius 18-5060). Using Octet buffer (PBS with 0.1% Tween20) ...
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bioRxiv - Cancer Biology 2021Quote: ... ICG powder was weighed and dissolved in cell culture medium (type of medium dependent on experiment) and sterile filtered (Sartorius Minisart High Flow, PES 0.22μm) to achieve a 100μM stock solution ...
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bioRxiv - Cell Biology 2022Quote: ... CM ultrafiltration and concentration was carried out by tangential flow filtration (TFF) using a Vivaflow 50 R 10,000 MWCO Hydrosart membrane (Sartorius, Göttingen, Germany) and a Masterflex L/S Easy Load machine (Antylia Scientific ...
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bioRxiv - Biochemistry 2021Quote: ... All protein samples were concentrated using Vivaspin centrifugal concentrators (Sartorius). Denaturing gel electrophoresis was performed using NuPAGE 4-12 % Bis-Tris gels (Thermo Fisher Scientific) ...
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bioRxiv - Cancer Biology 2022Quote: ... Then proteins were transferred into ultrafiltration tubes (10kDa, Sartorius AG) and washed three times with 50 mM NH4HCO3 at 14000g for 15 minutes ...
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bioRxiv - Biophysics 2023Quote: ... Purified proteins were concentrated using centrifugal filter devices (Vivaspin, Sartorius). Protein concentration was estimated from its absorbance at 280 nm ...
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bioRxiv - Biophysics 2023Quote: ... Protein samples were concentrated by ultrafiltration (5 kDa Vivaspin, Sartorius) and further purified by reverse phase chromatography using a preparative C4 column (Vydac 214 TP ...
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bioRxiv - Biochemistry 2023Quote: ... The cleaved protein was then concentrated (Vivaspin 30,000 MWCO, Sartorius) and ultracentrifuged in a TLA-120.2 rotor (Beckman Coulter ...
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bioRxiv - Bioengineering 2024Quote: ... the refolded protein was concentrated using a VIVAFLOW50 system (Sartorius). The concentrated sample was filtrated with 0.22 µm membrane filter ...
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bioRxiv - Biophysics 2023Quote: ... Protein samples were concentrated by ultrafiltration (5 kDa Vivaspin, Sartorius) and further purified by reverse phase chromatography using a preparative C4 column (Vydac 214 TP ...
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bioRxiv - Microbiology 2023Quote: We performed human plasma and avidity variant Abs’ binding using an Octet Red384 biolayer interferometer (Sartorius). We completed all the steps in 0.05% BSA and 0.002% Tween20 ...