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Citations for Sartorius :
151 - 171 of 171 citations for Recombinant Human PRMT1 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... fractions containing the protein of interest were pooled together and concentrated using Vivaspin 20 centrifugal concentrators with a 5 kDa cut-off (Sartorius). The concentration of BRD4 BD1 was determined spectrophotometrically at 280 nm using the calculated molar extinction coefficient ...
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bioRxiv - Biophysics 2023Quote: ... The flow-through volume containing the untagged protein was concentrated to 10 mg/mL using Vivacon500 filters (50 kDa, Sartorius) for further purification using a Superdex 200 Increase 5/150 GL column equilibrated in 50 mM Tris-HCl pH 7.0 ...
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bioRxiv - Biochemistry 2020Quote: ... Protein containing fractions were pooled and concentrated to 5 mg/ml in a Vivaspin 500 (3 kDa) centrifugal concentrator (Sartorius AG), after which they were aliquoted ...
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bioRxiv - Microbiology 2019Quote: ... Each mixture containing 100μg proteins was loaded onto a 10 kDa cut-off spin column (Vivacon 500, Sartorius AG, Goettingen, Germany). The lysate was adjusted to 8M urea by centrifuging to remove SDS and low-molecular-weight material ...
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bioRxiv - Biochemistry 2021Quote: ... The protein was concentrated to 25-30 µM with the use of a Centrisart I MWCO 100 kDa concentrator (Sartorius AG) and passed through a spin-out column of Bio-Gel P-6 equilibrated with the protein storage buffer (0.1 M Hepes-HCl ...
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bioRxiv - Cancer Biology 2024Quote: ... Glioma cells were stably transduced with a lentiviral nuclear red fluorescent protein under the EF1a promotor (Sartorius, cat. 4476, Göttingen, Germany) for visualization in live imaging assays ...
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bioRxiv - Microbiology 2019Quote: ... adapted by us to the use of 100 µg protein for digestion with sequencing-grade trypsin in 50:1 ratios in Vivacon 10k filters (Sartorius AG, Germany) (28) ...
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bioRxiv - Immunology 2019Quote: ... Worm culture supernatants collected over 1 week were further concentrated using centrifugal protein concentrators with a 5 kDa MWCO (Vivaspin, Sartorius, Göttingen, Germany) to obtain the final ...
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bioRxiv - Biochemistry 2020Quote: ... bengalensis protein hydrolysates (BBPH) were subjected to centrifugal ultrafiltration using 3kDa cut-off membrane filtration unit (Vivaspin20, VS2091, Sartorius AG, Goettingen, Germany) to separate the low molecular peptide fraction (< 3kDa) ...
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bioRxiv - Microbiology 2022Quote: The affinity between each antibody and spike protein was measured by bio-layer interferometry (BLI) with a BLItz Biolayer Interferometer System (Sartorius, Goettingen, Germany).
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bioRxiv - Plant Biology 2023Quote: The biolayer interferometry was performed as previously described (Xie et al., 2022) using Octet® R2 Protein Analysis System (Sartorius, Figure 1) or Gator-plus (Gator Bio ...
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bioRxiv - Plant Biology 2023Quote: ... Finally, protein concentration and buffer exchange (20 mM Tris-HCl, pH 7.5) was performed using Vivaspin 30K columns (Sartorius, Germany, Göttingen, Germany).
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bioRxiv - Biophysics 2023Quote: ... proteins were exchanged to labelling buffer (20 mM HEPES, 50 mM NaCl, pH 7.5) using VivaSpin 6 concentrator (Sartorius AG, Gottingen, Germany).
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bioRxiv - Bioengineering 2022Quote: ... and HT29 cells were transfected to express the nuclear-restricted mKate2 red fluorescent protein using the IncuCyte® NucLight Lentivirus Reagents (Sartorius, Cat: 4476) and following the manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: ... Protein production at pilot scale for nonclinical trial material was archived in 500L Bioreactor (BIOSTAT STR®500, Sartorius Stedim Biotech, Göttingen, Germany) using a fed-batch process using PowerCHO-2 medium as basal medium ...
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bioRxiv - Biochemistry 2020Quote: ... we concentrated the proteins from the supernate by membrane centrifugation using 5 K molecular weight cutoff Vivaspin units of 6 mL (Sartorius Stedim, Goettingen, Germany). The retentate (~300 μL ...
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bioRxiv - Biochemistry 2022Quote: ... The dialyzed 2C protein was centrifuged at 75,000 x g at 4 °C for 30 min to isolate soluble protein and concentrated using a Vivaspin Turbo 15 (Sartorius, 10 kDa MWCO) to 2 – 5 mL for preparative gel filtration ...
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bioRxiv - Microbiology 2023Quote: ... Fc-tagged RBD proteins (20 μg/mL) were captured on the protein A biosensor followed by 30 s of baseline run in 1x kinetics buffer (Sartorius Cat#: 18-1105). The biosensors were blocked with a control Fc protein (Jackson ImmunoResearch Cat# ...
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bioRxiv - Cancer Biology 2023Quote: ... of PD-L1 protein/PD-L1 antibody was determined by Octet Biolayer interferometry (BLI) using the Octet RED384 system (Sartorius, Bohemia, NY, USA). Briefly ...
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bioRxiv - Evolutionary Biology 2019Quote: ... The fractions containing MGE protein were pooled and concentrated to 1 mL by using a centrifugal concentrator (Sartorius, 10 kDa MWCO, Vivaspin turbo 15). The recombinant proteins were further purified by ion exchange and gel filtration chromatography with ÄKTAprime plus system ...
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bioRxiv - Evolutionary Biology 2019Quote: ... The fractions containing MGE protein were pooled and concentrated to 1 mL by using a centrifugal concentrator (Sartorius, 10 kDa MWCO, Vivaspin turbo 15). For gel filtration ...