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Citations for Sartorius :
1 - 50 of 222 citations for Recombinant Human LDLR protein GST tagged since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: KD measurements of Fc-tagged Human FcμR (Cat: 13556-H02H, SinoBiological) immobilised on Anti-Human IgG Fc (AHC) (Cat: 18-5060, Sartorius) biosensors were performed on the Octet Red96® system with the loading threshold set at 1.0 nm and utilizing the recombinant Pertuzumab and Trastuzumab IgM VH whole antibodies variants in five serial diluted concentrations (12.5 to 200nM ...
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bioRxiv - Immunology 2024Quote: ... GST-c-SLFN11 was immobilized onto Anti-GST Biosensor tips (Sartorius) in BLI buffer by dipping the biosensors into wells containing GST-c-SLFN11 for 300 s ...
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bioRxiv - Biophysics 2024Quote: ... His-tagged dROS1 protein (construct 2-3) were loaded onto penta-His sensors (Sartorius) to a response unit of about 0.5 nm followed by quenching in 10 ng/mL biocytin (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2022Quote: ... Purified His6-MBP-tagged proteins were then concentrated with a 30,000 MWCO centrifugal concentrator (Sartorius) and buffer exchanged into 20 mM HEPES pH 7.5 and 50 mM NaCl for crystallization ...
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bioRxiv - Biochemistry 2023Quote: ... Anti-GST biosensors (Sartorius #18-5096) were used to immobilise GST-Rgl2RA (provided as a 0.7 µM solution in the binding reservoir well ...
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bioRxiv - Microbiology 2020Quote: ... For all recombinant proteins,protein-containing fractions were pooled and buffer exchange to PBS via VivaSpin columns (Sartorius) was performed ...
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bioRxiv - Biochemistry 2024Quote: ... was carried out using GST-PHRF1RP and GST-PHRF1RP(P221L) and biotinylated unmodified nucleosomes (25 nM) immobilized on streptavidin biosensors (Sartorius). All samples were prepared in 20 mM MOPS (pH 7.0) ...
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bioRxiv - Immunology 2022Quote: Splenic CD4+ and CD8+ T-cells from Ldlr-/- and T-AbcdkoLdlr-/- mice were isolated and labeled using Incucyte Caspase3/7 Red Reagent (Sartorius). Cells were cultured in RPMI 1640 medium (Gibco ...
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bioRxiv - Cell Biology 2024Quote: ... This recombinant protein was biotinylated for further assessment using the avidin-utilizing Octet R2 (Sartorius, Germany).
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bioRxiv - Systems Biology 2022Quote: ... live-cell proliferation and expression of YFP-tagged proteins were tracked for 48 h using an Incucyte S3 device (Sartorius).
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bioRxiv - Genetics 2024Quote: ... and transferring LDLR-immobilized or control sensors to multiwell plates containing the indicated lipoprotein concentration in Octet Kinetics Buffer (Sartorius, 1#8-1105) at room temperature for 60 seconds followed by transfer to wells containing buffer only for an additional 180 seconds ...
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bioRxiv - Synthetic Biology 2022Quote: ... His-tagged antigens using Anti-Penta-HIS biosensors (Sartorius/ForteBio). Antigen was loaded to a threshold of 1 nm shift ...
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bioRxiv - Immunology 2021Quote: ... His-tagged ACE2 was immobilized onto Ni-NTA coated biosensors (Sartorius) before association of increasing concentrations of each RBD for 120 seconds followed by dissociation using HBS pH 7.4 for 360 seconds ...
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bioRxiv - Immunology 2024Quote: ... Biotinylated Avi tagged WA1 RBD was loaded onto Streptavidin sensor tips (Sartorius) to 2 nm that were pre- equilibrated in kinetic buffer A ...
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bioRxiv - Molecular Biology 2023Quote: ... The Hs53BP1 fragment without its GST tag was recovered in the flow through and concentrated using Vivaspin concentrator (Sartorius).
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bioRxiv - Cell Biology 2022Quote: ... or mCherry-tagged histone 2B (mCherry-H2B) were performed using an Incucyte SX5 (Sartorius Imaging). Images were taken once every 60 minutes to acquire videos of cells monitored over a 72-hour period.
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bioRxiv - Biochemistry 2021Quote: ... 12xHis-tagged EPCRWT or EPCRY154A was immobilized on the surface of NiNTA pre-coated biosensors (Sartorius), at a concentration of 100 µg/mL ...
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bioRxiv - Immunology 2021Quote: His-tagged FcαRI (Sinobiological, Cat: 10414-H08H) were immobilized via Ni-NTA biosensor (Sartorius, Cat: 18-5101) and subject to free floating IgA variants to obtained ka ...
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bioRxiv - Immunology 2024Quote: ... 222 nM of 6xHis-tagged BG505 SOSIP was immobilized with nickel-charges tris-NTA (Ni-NTA) biosensors (Sartorius) for 10 min and transferred to PBS for 1 min to wash off unbound SOSIP ...
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bioRxiv - Cell Biology 2024Quote: ... The transfection efficiency was measured using the TRPV1-GFP tagged transfection and Incucyte® Live-Cell Analysis (Sartorius) and routinely evaluated at 50% (Extended Data Fig ...
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bioRxiv - Biophysics 2024Quote: ... 4 μM of 6xHis-tagged ASF1A1-172 was immobilized on nickel-charged tris-nitriloacetic acid (Tris-NTA) biosensor (Sartorius) for the 90 seconds ...
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bioRxiv - Cell Biology 2022Quote: ... GCP6-tagged purified γ-TuRC was then subjected to buffer exchange using Vivaspin 500 centrifugal concentrator (10 kDa MWCO, Sartorius VS0102) for a final NaCl concentration of 150 mM in eluate ...
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bioRxiv - Biophysics 2024Quote: ... The protein elutes were concentrated using a protein concentrator (Sartorius #VS15T01). For protein labeling ...
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bioRxiv - Immunology 2022Quote: ... Anti-Human Fc sensor tips (ForteBio, now Sartorius) were loaded with 200 nM mAb at a threshold of 0.8nm and then submerged into wells containing 100 nM (protomer/monomer concentration ...
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bioRxiv - Biochemistry 2023Quote: ... Anti-Human Fc sensor tips (ForteBio, now Sartorius) were loaded with 200 nM mAb or FcACE2 to a threshold of 0.8 nm and then submerged into wells containing 100 nM (protomer/monomer concentration ...
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bioRxiv - Biophysics 2024Quote: ... The purified proteins were then concentrated using a protein concentrator (Sartorius #VS15T01) and verified by SDS-PAGE (Fig ...
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bioRxiv - Microbiology 2024Quote: ... protein A biosensors (Sartorius) were first loaded with 10 μg/mL of mAb/bsAb in the running buffer until a binding threshold of 1 nm was reached ...
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bioRxiv - Molecular Biology 2024Quote: ... Anti-human IgG Fc capture (AHC) biosensor tips (Sartorius) were equilibrated for ten minutes in a 1:4 mixture of conditioned Freestyle 293 medium and 1X HBS EP+ to match the diluted media ...
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bioRxiv - Microbiology 2024Quote: ... the anti-human IgG Fc capture (AHC) biosensor (Sartorius) was conditioned in the detergent-containing Kinetic Buffer (KB ...
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bioRxiv - Microbiology 2024Quote: Protein–protein interactions were measured using the Octet N1 system (Sartorius, Göttingen, Germany). His-SeFtsZ and His-SeSepF were diluted in BLI buffer (20 mM Tris-HCl pH 7.4 ...
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bioRxiv - Biophysics 2024Quote: ... The Cy5-labeled tau proteins were then concentrated using a protein concentrator (Sartorius #VS15T01).
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bioRxiv - Immunology 2020Quote: ... Protein A biosensors (FortéBio/Sartorius) were first hydrated for 10 minutes in Kinetics buffer ...
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bioRxiv - Biochemistry 2023Quote: ... each Protein A biosensors (Sartorius) was pre-equilibrated in PBST buffer (PBS ...
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bioRxiv - Immunology 2023Quote: ... Antibodies were captured on anti-human Fc AHC2 tips (Sartorius). Data capture was performed using Octet Data Acquisition software version 9.0 (ForteBio) ...
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bioRxiv - Immunology 2023Quote: The binding affinity of recombinant antibodies to antigens was estimated by bio-layer interferometry (Octet® QK, ForteBio/Sartorius). Antibodies were loaded at 10-20 nM onto anti-human IgG Fc capture or anti-Mouse IgG Fc capture biosensors ...
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bioRxiv - Cancer Biology 2023Quote: ... Its binding to LIF was confirmed using recombinant LIF (ACRO Biosystems, LIF-H52H3) on an Octet RED 96e biolayer interferometry instrument (Sartorius).
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bioRxiv - Molecular Biology 2023Quote: Human NIL iPSCs were cultured in Nutristem (Sartorius, #05-100-1A) supplemented with Pen/Strep 0.1% on geltrex-coated plates (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2022Quote: ... Protein A biosensors (Sartorius, #18-5010) were hydrated in BLI assay buffer for 10-20 minutes and loaded onto an Octet Red96 BLI instrument (Pall ...
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bioRxiv - Immunology 2021Quote: ... recombinant SARS-CoV-2 S2-His-tag at 10 µg/mL was loaded on Anti-Penta-HIS (HIS1K) biosensors (Sartorius Corporation) for 1 min ...
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bioRxiv - Pathology 2024Quote: Human bone specimens were homogenized by Mikro-Dismembrator U (Sartorius, Gottingen, Germany) and total RNA was isolated using the TRI Reagent (ThermoFisher Scientific ...
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bioRxiv - Neuroscience 2020Quote: ... using protein concentrator tubes (VIVASPIN 20, Sartorius). Both purified IgG fractions from patient and controls were re-tested on CBAs and the anti-IgLON5 IgG concentration was determined (titer 1:100) ...
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bioRxiv - Cancer Biology 2020Quote: Proteins were transferred to nitrocellulose membrane (Sartorius). The membranes were blocked with 10% BSA (Santa Cruz Biotechnology ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins were concentrated using centrifugal concentrator (Sartorius) and further purified with size exclusion chromatography using a Superdex 200 column (GE Life Sciences) ...
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bioRxiv - Cancer Biology 2021Quote: ... Beads detecting cytokines from human were analyzed on an IQue Screener Plus (Sartorius). All assays were analyzed using quintuplicate determinations.
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bioRxiv - Evolutionary Biology 2022Quote: ... IgG was loaded onto Anti-Human Fc Capture (AHC) biosensors (Sartorius, #18-5060). To reduce the avidity effect ...
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bioRxiv - Immunology 2024Quote: Purified IgG1 mAbs were quantified using anti–human Fab-CH1 (FAB2G) biosensors (Sartorius) as previously described (58) ...
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bioRxiv - Microbiology 2023Quote: ... The eluted proteins were concentrated with a centrifugal device (MWCO 30 kDa for soluble EFNB2 proteins and 50 kDa for soluble Eph receptor proteins; Sartorius) before being separated on a Superdex 200 Increase 10/300 GL (Cytiva Life Sciences ...
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bioRxiv - Biochemistry 2020Quote: ... Proteins were concentrated using Vivaspin centrifugal filters (Sartorius) of appropriate cut-offs ...
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bioRxiv - Biochemistry 2020Quote: Purified proteins were concentrated with VIVASPIN concentrators (Sartorius) and dialyzed against E-Buffer with 1 mM DTT ...
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bioRxiv - Biochemistry 2022Quote: ... using a 100kDa MWCO Vivaspin protein concentrator (Sartorius), aliquoted ...