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Citations for Sartorius :
101 - 150 of 214 citations for Recombinant Human LDLR Protein His Avi tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2023Quote: ... Antibody CSL362 hIgG1 (captured ligand) was captured by Anti-Human Fc capture biosensor (AHC) (Sartorius, PN: 18-5060) for 300s at 0.5µg/mL ...
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bioRxiv - Biochemistry 2020Quote: ... Protein was concentrated using a Vivaspin centrifugal concentrator (MWCO 10 kDa, Sartorius) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... The protein was concentrated with Vivaspin 20 concentrators (30 kDa MWCO, Sartorius). Concentrated protein was further purified by size exclusion chromatography on a HiLoad 16/600 Superdex 200 pg column (Cytiva) ...
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bioRxiv - Molecular Biology 2022Quote: ... the proteins were centrifuged on a 30 kDa cutoff filter device (Sartorius) and washed each thrice with UA buffer (8 M urea in 0.1 M Tris/HCl pH 8.5 ...
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bioRxiv - Biochemistry 2023Quote: ... Dialysed protein was spin-concentrated in a Vivaspin 6 MWCO 50,000 (Sartorius) to the desired concentration ...
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bioRxiv - Microbiology 2024Quote: ... The proteins were concentrated using a Vivaspin6 10 kDa filter unit (Sartorius). The protein concentration was measured via absorption at 280 nm using a Nanodrop spectrophotometer (ThermoFisher ...
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bioRxiv - Immunology 2023Quote: ... Human antibodies (5 μg/mL or 7.5 µg/mL) were captured on pre-conditioned anti-human IgG Fc-capture biosensors (AHC, Sartorius) for 600 s ...
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bioRxiv - Cell Biology 2022Quote: ... The eluted protein was adjusted to desired concentration via Vivaspin (Sartorius, Göttingen, Germany), analyzed by SDS-PAGE and snap frozen in liquid nitrogen ...
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bioRxiv - Neuroscience 2021Quote: ... followed by centrifugation of denatured proteins on a filter device (Sartorius, Vivacon 500). After several washing steps with 8 M urea and ammonium bicarbonate ...
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bioRxiv - Biochemistry 2021Quote: ... The immobilization of ligand proteins on amine reactive second-generation (AR2G) biosensors (Sartorius) was done following manufacturer’s instruction ...
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bioRxiv - Microbiology 2021Quote: ... Protein samples were diluted to 100 nM in Kinetics buffer (Pall FortéBio/Sartorius). Buffer ...
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bioRxiv - Molecular Biology 2022Quote: ... The protein was concentrated using ultrafiltration through a 30 kDa cutoff filter (Sartorius), snap-frozen and stored at −80 °C until further used.
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bioRxiv - Molecular Biology 2022Quote: ... The protein was concentrated using ultrafiltration through a 50 kDa cutoff filter (Sartorius), snap-frozen and stored at −80 °C until further use ...
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bioRxiv - Synthetic Biology 2024Quote: ... and aqueous solutions containing protein components were filtered using Minisart syringe filters (Sartorius). 0.5x1mm (I.DxO.D. ...
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bioRxiv - Microbiology 2022Quote: ... and 0.5% nonfat dry milk) to 100 nM and immobilized for 60 s to anti-human IgG Fc capture biosensors (Sartorius). Sensors were transferred to assay buffer for 30 s to set the baseline ...
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bioRxiv - Biochemistry 2020Quote: Digestion of 10His-SUMO1T95R proteins was performed on 10 kDa-cutoff spin filters (Sartorius) according to a published protocol ...
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bioRxiv - Cell Biology 2020Quote: ... the protein was simultaneously concentrated and buffer-exchanged (with a Vivaspin Turbo 4; Sartorius) into PM buffer (80 mM Pipes pH 6.8 ...
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bioRxiv - Plant Biology 2023Quote: ... Protein extracts were filtered using Minisart 0.45 μm filter (Sartorius Stedim Biotech, Goettingen, Germany). Part of the extract was set aside prior to immunoprecipitation and used for SDS-PAGE as described above ...
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bioRxiv - Bioengineering 2023Quote: ... The AFC-eluates were buffer exchanged with 10kDa Vivaspin protein concentrator (Sartorius Stedim, Germany) using Sorval Lynx XTR centrifuge (Thermo Scientific ...
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bioRxiv - Biophysics 2023Quote: ... Proteins solutions were concentrated to at least 600 µM with Vivaspin centrifugal concentrators (Sartorius) and stored at -80 °C.
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bioRxiv - Plant Biology 2023Quote: ... Proteins eluting from the column were concentrated by ultrafiltration on Vivaspin 20 columns (Sartorius) with a 5 kDa molecular weight cut-off ...
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bioRxiv - Neuroscience 2024Quote: ... and Ulla proteins were concentrated to 35 ∼ 50 mg/ml using a VIVASPIN20 (Sartorius) concentrator and stored at −80 °C until use.
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bioRxiv - Biochemistry 2024Quote: ... Anti-FLAG M2 was immobilized on protein G biosensors (Sartorius Cat. No. 18-5082) at 5 µg/mL (in binding buffer ...
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bioRxiv - Immunology 2023Quote: All BLI for data shown in Figure 1 and Figure S1 was carried out on an OctetRED384 (ForteBio) using anti-human Fc-capture sensors (Sartorius, 18-5060) to immobilize anti-PfRH5 mAbs ...
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bioRxiv - Microbiology 2020Quote: ... Samples containing purified protein were concentrated using Vivaspin® 6 5,000 MWCO columns (Sartorius Stedim). Protein was buffer exchanged using membrane filtration into 50 mM NaP ...
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bioRxiv - Immunology 2022Quote: ... Purified protein was buffer exchanged into PBS and concentrated using a Vivaspin concentrator (Sartorius-Stedim) to 3-5 mg/mL and flash-frozen with liquid nitrogen and stored at −80 °C.
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bioRxiv - Molecular Biology 2021Quote: ... Remaining dye was washed away by concentrating protein using Vivaspin® 500 centrifugal concentrators (Sartorius) into MST buffer (10 mM HEPES pH 7.5 ...
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bioRxiv - Biochemistry 2022Quote: ... Protein complexes were concentrated to 25 µL in 30 kDa centrifugal concentrator (Vivaspin 500 Sartorius) and mixed with equal volume of 2 x native gel sample buffer (62.5 mM Tris-HCl ...
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bioRxiv - Biophysics 2022Quote: ... The protein solution was first concentrated by crossflow filtration using a Vivaflow 200 system (Sartorius) and two Hydrosart cassettes (MWCO ...
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bioRxiv - Biophysics 2022Quote: ... The protein was concentrated to the desired concentration using a Vivaspin Turbo centrifugal concentrator (Sartorius) with a 3 kDa membrane by centrifugation at 4000 g and 4 °C ...
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bioRxiv - Biochemistry 2023Quote: ... Eluted proteins were concentrated using Vivaspin® 10,000 MWCO centrifugal filter units (Sartorius, Göttingen, Germany). Proteins were purified to homogeneity using a size-exclusion chromatography column (HiLoad® Superdex ...
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bioRxiv - Microbiology 2023Quote: ... The proteins were spin concentrated to 5 mg / ml (3 kDa MWCO, vivaspin 20, Sartorius), aliquoted ...
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bioRxiv - Physiology 2023Quote: EV suspensions containing 50 µg protein were digested using FASP digestion (Vacacon500, Sartorius, VN01H02 10kDA), and sample volumes were adjusted to 100 µL with 8M urea ...
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bioRxiv - Biophysics 2019Quote: ... The protein was concentrated to 0.5 ml with a 100-kDa-cutoff concentrator (Vivaspin 20, Sartorius), and further purified by SEC as above.
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bioRxiv - Plant Biology 2021Quote: ... and concentrated to the indicated total protein concentration (Vivaspin 20, 30 000 MW cut-off, Sartorius). For depletion assay ...
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bioRxiv - Cell Biology 2022Quote: ... Protein was concentrated from the media using VivaSpin Turbo 4 (10000 MWCO) concentrator tubes (Sartorius, VS04T01). Protein samples were run on 4-15% Mini protean TGX stain-free gels (Bio-Rad ...
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bioRxiv - Molecular Biology 2021Quote: ... loaded onto Vivaspin protein concentrator columns with a molecular weight cut-off of 30 kDa (Sartorius) and centrifuged at 4,000 x g at 4 °C until a concentration factor of 20 was achieved ...
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bioRxiv - Immunology 2022Quote: ... Protein-containing fractions were pooled and rebuffered to PBS using VIVAspin columns (5,000 MWCO PES; Sartorius). Protein yield was determined with a BCA protein assay (Pierce™ ...
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bioRxiv - Molecular Biology 2021Quote: ... The protein was concentrated to 3 mg/ml using a 50 kDa cutoff concentrator (Vivaspin, Sartorius). The purified protein was flash frozen in liquid nitrogen and stored at − 80 °C.
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bioRxiv - Plant Biology 2021Quote: ... Proteins eluting from the column were concentrated by ultrafiltration on Vivaspin 20 and 2 columns (Sartorius) with a 5 kDa molecular weight cut-off ...
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bioRxiv - Molecular Biology 2021Quote: ... The proteins were concentrated with Vivaspin 20-mL centrifugal devices with 5,000 or 10,000 MWCO (Sartorius) and buffer-exchanged into NMR buffer over PD-10 gel-filtration columns (GE-healthcare).
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bioRxiv - Biochemistry 2022Quote: ... Protein was pooled and concentrated with a Vivaspin 30 kDa molecular weight cut-off centricon (Sartorius) and loaded onto a buffer A equilibrated S6 Increase 3.2/300 (Cytiva ...
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bioRxiv - Microbiology 2023Quote: ... The tag-free RnpM protein was collected and concentrated using a Vivaspin turbo 15 (Sartorius, Germany).
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bioRxiv - Molecular Biology 2023Quote: ... followed by protein concentration using vivaspin centrifugal concentrators columns (MWCO 3.0 kD, Sartorius GmbH, Göttingen, Germany). Protein contents were analyzed according to Bradford using Rotiquant (Roth ...
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bioRxiv - Microbiology 2024Quote: ... Protein-containing fractions were pooled and concentrated in a Vivaspin 20 centrifugal concentrator (50000 MWCO; Sartorius). Purified complexes were analyzed by spectrophotometry (Nanodrop ...
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bioRxiv - Microbiology 2023Quote: ... MTSL-labelled protein was concentrated (Vivaspin® 500 centrifugal filter, 10 kDa MWCO PES membrane, Sartorius). Labelling efficiencies for each Csx23 mutant were assessed by continuous wave (CW ...
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bioRxiv - Microbiology 2020Quote: ... The purified protein was buffer exchanged into PBS using a Vivacon 500 concentrator (Cat: VS0122, Sartorius Stedim). For the generation of human ACE2-hFc and SARS-CoV-2 RBD-mFc recombinant proteins ...
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bioRxiv - Immunology 2021Quote: Biolayer interferometry was performed using the Octet Red96 with Protein A (ProA) biosensors (Sartorius ForteBio, Fremont, CA), which were hydrated for 15 min in kinetics buffer prior to each run ...
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bioRxiv - Microbiology 2021Quote: ... the purified proteins were run on Superdex S200 size-exclusion column and concentrated with Vivaspin devices (Sartorius).
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bioRxiv - Biochemistry 2022Quote: ... Buffer exchange and protein sample concentration were performed using 10 kDa MWCO Vivaspin Turbo centricons (Sartorius, Germany). After IMAC ...