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Citations for Sartorius :
1 - 50 of 327 citations for Recombinant Human ERBB2 protein Fc tagged R PE labeled since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: KD measurements of Fc-tagged Human FcμR (Cat: 13556-H02H, SinoBiological) immobilised on Anti-Human IgG Fc (AHC) (Cat: 18-5060, Sartorius) biosensors were performed on the Octet Red96® system with the loading threshold set at 1.0 nm and utilizing the recombinant Pertuzumab and Trastuzumab IgM VH whole antibodies variants in five serial diluted concentrations (12.5 to 200nM ...
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bioRxiv - Immunology 2022Quote: ... Anti-Human Fc sensor tips (ForteBio, now Sartorius) were loaded with 200 nM mAb at a threshold of 0.8nm and then submerged into wells containing 100 nM (protomer/monomer concentration ...
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bioRxiv - Biochemistry 2023Quote: ... Anti-Human Fc sensor tips (ForteBio, now Sartorius) were loaded with 200 nM mAb or FcACE2 to a threshold of 0.8 nm and then submerged into wells containing 100 nM (protomer/monomer concentration ...
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bioRxiv - Microbiology 2024Quote: ... we immobilized ACE2 fused to the human Fc domain (sACE2-Fc) onto Anti-hIgG Fc Capture (AHC) Biosensors (Sartorius) at 5 µg/ml for 10 minutes ...
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bioRxiv - Molecular Biology 2024Quote: ... Anti-human IgG Fc capture (AHC) biosensor tips (Sartorius) were equilibrated for ten minutes in a 1:4 mixture of conditioned Freestyle 293 medium and 1X HBS EP+ to match the diluted media ...
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bioRxiv - Microbiology 2024Quote: ... the anti-human IgG Fc capture (AHC) biosensor (Sartorius) was conditioned in the detergent-containing Kinetic Buffer (KB ...
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bioRxiv - Immunology 2023Quote: ... Antibodies were captured on anti-human Fc AHC2 tips (Sartorius). Data capture was performed using Octet Data Acquisition software version 9.0 (ForteBio) ...
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bioRxiv - Evolutionary Biology 2022Quote: ... IgG was loaded onto Anti-Human Fc Capture (AHC) biosensors (Sartorius, #18-5060). To reduce the avidity effect ...
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bioRxiv - Biophysics 2024Quote: ... The Cy5-labeled tau proteins were then concentrated using a protein concentrator (Sartorius #VS15T01).
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bioRxiv - Biophysics 2024Quote: ... His-tagged dROS1 protein (construct 2-3) were loaded onto penta-His sensors (Sartorius) to a response unit of about 0.5 nm followed by quenching in 10 ng/mL biocytin (Sigma-Aldrich ...
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bioRxiv - Immunology 2023Quote: ... BLI binding experiments were performed with Anti Human IgG Fc (AHC) biosensors (Sartorius 18-5060). Using Octet buffer (PBS with 0.1% Tween20) ...
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bioRxiv - Biochemistry 2022Quote: ... Purified His6-MBP-tagged proteins were then concentrated with a 30,000 MWCO centrifugal concentrator (Sartorius) and buffer exchanged into 20 mM HEPES pH 7.5 and 50 mM NaCl for crystallization ...
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bioRxiv - Microbiology 2020Quote: ... For all recombinant proteins,protein-containing fractions were pooled and buffer exchange to PBS via VivaSpin columns (Sartorius) was performed ...
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bioRxiv - Immunology 2024Quote: ... Monoclonal Moo1 and Moo2 antibodies were immobilized on anti-human IgG Fc Capture (AHC) biosensors (Sartorius) to capture monoclonal antibodies at a concentration of 50 µg/mL for 5 min and washed with octet buffer ...
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bioRxiv - Bioengineering 2023Quote: ... Antibody CSL362 hIgG1 (captured ligand) was captured by Anti-Human Fc capture biosensor (AHC) (Sartorius, PN: 18-5060) for 300s at 0.5µg/mL ...
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bioRxiv - Immunology 2023Quote: ... Human antibodies (5 μg/mL or 7.5 µg/mL) were captured on pre-conditioned anti-human IgG Fc-capture biosensors (AHC, Sartorius) for 600 s ...
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bioRxiv - Microbiology 2024Quote: ... RHA10.04 or RHA10 UCA IgG was diluted in PBST with 1% BSA to a concentration of 5µg/ml and captured on anti-human IgG-Fc biosensors (Sartorius) for 300s ...
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bioRxiv - Microbiology 2024Quote: ... PES (Sartorius). The test media composition can be seen in Supplementary Table S1 ...
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bioRxiv - Cell Biology 2024Quote: ... This recombinant protein was biotinylated for further assessment using the avidin-utilizing Octet R2 (Sartorius, Germany).
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bioRxiv - Microbiology 2022Quote: ... and 0.5% nonfat dry milk) to 100 nM and immobilized for 60 s to anti-human IgG Fc capture biosensors (Sartorius). Sensors were transferred to assay buffer for 30 s to set the baseline ...
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bioRxiv - Immunology 2022Quote: ... Protein-containing fractions were pooled and rebuffered to PBS using VIVAspin columns (5,000 MWCO PES; Sartorius). Protein yield was determined with a BCA protein assay (Pierce™ ...
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bioRxiv - Microbiology 2023Quote: ... MTSL-labelled protein was concentrated (Vivaspin® 500 centrifugal filter, 10 kDa MWCO PES membrane, Sartorius). Labelling efficiencies for each Csx23 mutant were assessed by continuous wave (CW ...
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bioRxiv - Systems Biology 2022Quote: ... live-cell proliferation and expression of YFP-tagged proteins were tracked for 48 h using an Incucyte S3 device (Sartorius).
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bioRxiv - Immunology 2022Quote: ... Anti-hIgG Fc capture (AHC) or anti-mIgG Fc capture sensors (Sartorius) were functionalized with monoclonal antibodies ...
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bioRxiv - Biophysics 2024Quote: ... The labeled protein was concentrated to the desired concentration using Vivaspin Turbo 4 10000 MWCO concentrators (Sartorius). The protein concentration was determined with the formula to account for the absorption of fluorescein at 280 nm (https://www.aatbio.com/resources/correction-factor/fluorescein).
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bioRxiv - Biochemistry 2024Quote: ... Human TLR3-FC (Acro Biosystems) was diluted to 0.01 mg/mL in kinetics buffer and loaded onto Protein A tips (Sartorius). The cell assay constructs were diluted to 10 μM in kinetics buffer and their association was measured for 300 s ...
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bioRxiv - Immunology 2023Quote: All BLI for data shown in Figure 1 and Figure S1 was carried out on an OctetRED384 (ForteBio) using anti-human Fc-capture sensors (Sartorius, 18-5060) to immobilize anti-PfRH5 mAbs ...
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bioRxiv - Microbiology 2020Quote: ... 10.000 MWCO PES (Sartorius). Finally ...
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bioRxiv - Neuroscience 2022Quote: ... PES spin filters (Sartorius), then transferred to autosampler vials with 175μL inserts for analysis.
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bioRxiv - Biochemistry 2024Quote: ... the protein was transferred to a Vivaspin® 6 centrifuge unit with a 100 kDa MWCO PES membrane (Sartorius).
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bioRxiv - Microbiology 2022Quote: ... The peak fractions corresponding to the protein complex were concentrated to about 16-20 mg/ml using a centrifugal filter with a PES membrane (Sartorius) and used for preparation of cryo-EM sample grids immediately.
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bioRxiv - Molecular Biology 2023Quote: ... 10,000 MWCO PES columns (Sartorius). In brief ...
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bioRxiv - Synthetic Biology 2022Quote: ... His-tagged antigens using Anti-Penta-HIS biosensors (Sartorius/ForteBio). Antigen was loaded to a threshold of 1 nm shift ...
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bioRxiv - Neuroscience 2020Quote: ... filtered using 0.4μm PES filters (Sartorius), concentrated 50-100 times and dialyzed into sterile Dulbecco’s phosphate buffer saline (DPBS ...
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bioRxiv - Biochemistry 2020Quote: ... 10 kDa MWCO PES membranes (Sartorius); supplemented by NaCl ...
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bioRxiv - Immunology 2021Quote: ... His-tagged ACE2 was immobilized onto Ni-NTA coated biosensors (Sartorius) before association of increasing concentrations of each RBD for 120 seconds followed by dissociation using HBS pH 7.4 for 360 seconds ...
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bioRxiv - Cancer Biology 2023Quote: ... and labeled using pHrodo red (Sartorius) at a final concentration of 31.3 ng/mL for 45 minutes at 37°C in pHrodo Labeling Buffer (Sartorius) ...
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bioRxiv - Microbiology 2022Quote: ... 10,000 MWCO PES concentrator (Sartorius, catalog # VS0201) and Nanosep centrifugal devices with Omega membrane at MWCO of 30K (Pall ...
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bioRxiv - Microbiology 2022Quote: ... 5000 MWCO PES (Sartorius AG, Göttigen, Germany) to 2 ml and separated using a HiLoad 16/60 Superdex 75 prep grade (Cytiva Europe GmbH ...
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bioRxiv - Immunology 2024Quote: ... Biotinylated Avi tagged WA1 RBD was loaded onto Streptavidin sensor tips (Sartorius) to 2 nm that were pre- equilibrated in kinetic buffer A ...
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bioRxiv - Bioengineering 2023Quote: ... Mouse Fc-capture biosensor tips were purchased from Sartorius. Chicken plasma (cat # ...
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bioRxiv - Cancer Biology 2024Quote: Bladder cancer cells labeled with NucLightRed (Sartorius) were co-cultured with human non-transduced (NTD ...
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bioRxiv - Bioengineering 2022Quote: ... a 100 kDa MWCO Vivaspin PES filter (Sartorius) was pre-rinsed with deionized water to ensure full saturation of the filter membrane ...
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bioRxiv - Biochemistry 2022Quote: ... The clear supernatant was microfiltered (0.22 µm, PES) and concentrated to 50 mL using a Sartorius Vivacell 250 PES Centrifugal Concentrator (Sartorius, Göttingen, Germany) with a MWCO of 100 kDa and a pressure of 4 bar ...
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bioRxiv - Biochemistry 2022Quote: ... using a 100-kDa MWCO Vivaspin Turbo PES (Sartorius) concentrator.
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bioRxiv - Immunology 2024Quote: ... and then immobilized onto Anti-mIgG Fc Capture (AMC) biosensors (Sartorius). After a 60-seconds washing step with PBST ...
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bioRxiv - Cell Biology 2022Quote: ... or mCherry-tagged histone 2B (mCherry-H2B) were performed using an Incucyte SX5 (Sartorius Imaging). Images were taken once every 60 minutes to acquire videos of cells monitored over a 72-hour period.
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Micro RNAs are minor constituents of extracellular vesicles and are hardly delivered to target cellsbioRxiv - Cell Biology 2020Quote: ... and then filtered using a 300K Vivaspin 20 (PES, Sartorius) device at 2,000 g at 10 °C for 20–30 min ...
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bioRxiv - Molecular Biology 2022Quote: ... with Vivaflow 200 10,000 MWCO PES filters (Sartorius, NY, USA). Dm III-2 and Dm III-2 + cys were purified using Ni-NTA resin as described for Dm IV recombinant proteins (Suppl ...
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bioRxiv - Cancer Biology 2024Quote: ... aggregates removed by passing through 0.4 µm PES filter (Sartorius) and sample incubated with 2 NiNTA resin (Qiagen ...