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Citations for Sartorius :
101 - 150 of 205 citations for Recombinant Human CD276 Protein Fc Avi tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... followed by centrifugation of denatured proteins on a filter device (Sartorius, Vivacon 500). After several washing steps with 8 M urea and ammonium bicarbonate ...
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bioRxiv - Biochemistry 2021Quote: ... The immobilization of ligand proteins on amine reactive second-generation (AR2G) biosensors (Sartorius) was done following manufacturer’s instruction ...
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bioRxiv - Microbiology 2021Quote: ... Protein samples were diluted to 100 nM in Kinetics buffer (Pall FortéBio/Sartorius). Buffer ...
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bioRxiv - Molecular Biology 2022Quote: ... The protein was concentrated using ultrafiltration through a 30 kDa cutoff filter (Sartorius), snap-frozen and stored at −80 °C until further used.
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bioRxiv - Molecular Biology 2022Quote: ... The protein was concentrated using ultrafiltration through a 50 kDa cutoff filter (Sartorius), snap-frozen and stored at −80 °C until further use ...
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bioRxiv - Synthetic Biology 2024Quote: ... and aqueous solutions containing protein components were filtered using Minisart syringe filters (Sartorius). 0.5x1mm (I.DxO.D. ...
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bioRxiv - Biochemistry 2020Quote: Digestion of 10His-SUMO1T95R proteins was performed on 10 kDa-cutoff spin filters (Sartorius) according to a published protocol ...
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bioRxiv - Cell Biology 2020Quote: ... the protein was simultaneously concentrated and buffer-exchanged (with a Vivaspin Turbo 4; Sartorius) into PM buffer (80 mM Pipes pH 6.8 ...
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bioRxiv - Plant Biology 2023Quote: ... Protein extracts were filtered using Minisart 0.45 μm filter (Sartorius Stedim Biotech, Goettingen, Germany). Part of the extract was set aside prior to immunoprecipitation and used for SDS-PAGE as described above ...
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bioRxiv - Bioengineering 2023Quote: ... The AFC-eluates were buffer exchanged with 10kDa Vivaspin protein concentrator (Sartorius Stedim, Germany) using Sorval Lynx XTR centrifuge (Thermo Scientific ...
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bioRxiv - Plant Biology 2023Quote: ... Proteins eluting from the column were concentrated by ultrafiltration on Vivaspin 20 columns (Sartorius) with a 5 kDa molecular weight cut-off ...
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bioRxiv - Biophysics 2023Quote: ... Proteins solutions were concentrated to at least 600 µM with Vivaspin centrifugal concentrators (Sartorius) and stored at -80 °C.
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bioRxiv - Neuroscience 2024Quote: ... and Ulla proteins were concentrated to 35 ∼ 50 mg/ml using a VIVASPIN20 (Sartorius) concentrator and stored at −80 °C until use.
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bioRxiv - Biochemistry 2024Quote: ... Anti-FLAG M2 was immobilized on protein G biosensors (Sartorius Cat. No. 18-5082) at 5 µg/mL (in binding buffer ...
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bioRxiv - Microbiology 2020Quote: ... Samples containing purified protein were concentrated using Vivaspin® 6 5,000 MWCO columns (Sartorius Stedim). Protein was buffer exchanged using membrane filtration into 50 mM NaP ...
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bioRxiv - Immunology 2022Quote: ... Purified protein was buffer exchanged into PBS and concentrated using a Vivaspin concentrator (Sartorius-Stedim) to 3-5 mg/mL and flash-frozen with liquid nitrogen and stored at −80 °C.
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bioRxiv - Molecular Biology 2021Quote: ... Remaining dye was washed away by concentrating protein using Vivaspin® 500 centrifugal concentrators (Sartorius) into MST buffer (10 mM HEPES pH 7.5 ...
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bioRxiv - Biochemistry 2022Quote: ... Protein complexes were concentrated to 25 µL in 30 kDa centrifugal concentrator (Vivaspin 500 Sartorius) and mixed with equal volume of 2 x native gel sample buffer (62.5 mM Tris-HCl ...
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bioRxiv - Biophysics 2022Quote: ... The protein solution was first concentrated by crossflow filtration using a Vivaflow 200 system (Sartorius) and two Hydrosart cassettes (MWCO ...
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bioRxiv - Biophysics 2022Quote: ... The protein was concentrated to the desired concentration using a Vivaspin Turbo centrifugal concentrator (Sartorius) with a 3 kDa membrane by centrifugation at 4000 g and 4 °C ...
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bioRxiv - Biochemistry 2023Quote: ... Eluted proteins were concentrated using Vivaspin® 10,000 MWCO centrifugal filter units (Sartorius, Göttingen, Germany). Proteins were purified to homogeneity using a size-exclusion chromatography column (HiLoad® Superdex ...
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bioRxiv - Microbiology 2023Quote: ... The proteins were spin concentrated to 5 mg / ml (3 kDa MWCO, vivaspin 20, Sartorius), aliquoted ...
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bioRxiv - Physiology 2023Quote: EV suspensions containing 50 µg protein were digested using FASP digestion (Vacacon500, Sartorius, VN01H02 10kDA), and sample volumes were adjusted to 100 µL with 8M urea ...
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bioRxiv - Biophysics 2019Quote: ... The protein was concentrated to 0.5 ml with a 100-kDa-cutoff concentrator (Vivaspin 20, Sartorius), and further purified by SEC as above.
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bioRxiv - Plant Biology 2021Quote: ... and concentrated to the indicated total protein concentration (Vivaspin 20, 30 000 MW cut-off, Sartorius). For depletion assay ...
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bioRxiv - Cell Biology 2022Quote: ... Protein was concentrated from the media using VivaSpin Turbo 4 (10000 MWCO) concentrator tubes (Sartorius, VS04T01). Protein samples were run on 4-15% Mini protean TGX stain-free gels (Bio-Rad ...
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bioRxiv - Molecular Biology 2021Quote: ... loaded onto Vivaspin protein concentrator columns with a molecular weight cut-off of 30 kDa (Sartorius) and centrifuged at 4,000 x g at 4 °C until a concentration factor of 20 was achieved ...
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bioRxiv - Immunology 2022Quote: ... Protein-containing fractions were pooled and rebuffered to PBS using VIVAspin columns (5,000 MWCO PES; Sartorius). Protein yield was determined with a BCA protein assay (Pierce™ ...
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bioRxiv - Molecular Biology 2021Quote: ... The protein was concentrated to 3 mg/ml using a 50 kDa cutoff concentrator (Vivaspin, Sartorius). The purified protein was flash frozen in liquid nitrogen and stored at − 80 °C.
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bioRxiv - Plant Biology 2021Quote: ... Proteins eluting from the column were concentrated by ultrafiltration on Vivaspin 20 and 2 columns (Sartorius) with a 5 kDa molecular weight cut-off ...
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bioRxiv - Molecular Biology 2021Quote: ... The proteins were concentrated with Vivaspin 20-mL centrifugal devices with 5,000 or 10,000 MWCO (Sartorius) and buffer-exchanged into NMR buffer over PD-10 gel-filtration columns (GE-healthcare).
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bioRxiv - Biochemistry 2022Quote: ... Protein was pooled and concentrated with a Vivaspin 30 kDa molecular weight cut-off centricon (Sartorius) and loaded onto a buffer A equilibrated S6 Increase 3.2/300 (Cytiva ...
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bioRxiv - Microbiology 2023Quote: ... The tag-free RnpM protein was collected and concentrated using a Vivaspin turbo 15 (Sartorius, Germany).
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bioRxiv - Molecular Biology 2023Quote: ... followed by protein concentration using vivaspin centrifugal concentrators columns (MWCO 3.0 kD, Sartorius GmbH, Göttingen, Germany). Protein contents were analyzed according to Bradford using Rotiquant (Roth ...
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bioRxiv - Microbiology 2023Quote: ... MTSL-labelled protein was concentrated (Vivaspin® 500 centrifugal filter, 10 kDa MWCO PES membrane, Sartorius). Labelling efficiencies for each Csx23 mutant were assessed by continuous wave (CW ...
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bioRxiv - Microbiology 2024Quote: ... Protein-containing fractions were pooled and concentrated in a Vivaspin 20 centrifugal concentrator (50000 MWCO; Sartorius). Purified complexes were analyzed by spectrophotometry (Nanodrop ...
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bioRxiv - Microbiology 2020Quote: ... The purified protein was buffer exchanged into PBS using a Vivacon 500 concentrator (Cat: VS0122, Sartorius Stedim). For the generation of human ACE2-hFc and SARS-CoV-2 RBD-mFc recombinant proteins ...
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bioRxiv - Immunology 2021Quote: Biolayer interferometry was performed using the Octet Red96 with Protein A (ProA) biosensors (Sartorius ForteBio, Fremont, CA), which were hydrated for 15 min in kinetics buffer prior to each run ...
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bioRxiv - Microbiology 2021Quote: ... the purified proteins were run on Superdex S200 size-exclusion column and concentrated with Vivaspin devices (Sartorius).
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bioRxiv - Biochemistry 2022Quote: ... Buffer exchange and protein sample concentration were performed using 10 kDa MWCO Vivaspin Turbo centricons (Sartorius, Germany). After IMAC ...
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bioRxiv - Molecular Biology 2021Quote: Biolayer interferometry was performed using the Octet Red96 with Protein A (ProA) biosensors (Sartorius ForteBio, Fremont, CA), which were hydrated for 15 min in kinetics buffer prior to each run ...
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bioRxiv - Biophysics 2022Quote: ... Dialyzed protein was then concentrated with 10 kDa MWCO Vivaspin centrifugal concentrators (3,500 xg, 4°C) (Sartorius). Protein concentrations were determined by means of fluorescence using an sfGFP calibration curve ...
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bioRxiv - Bioengineering 2022Quote: Biolayer interferometry was performed using the Octet Red96 with Protein A (ProA) biosensors (Sartorius ForteBio, Fremont, CA), which were hydrated for 15 min in kinetics buffer prior to each run ...
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bioRxiv - Immunology 2022Quote: The Octet quantification assay for protein expression level was performed on an Octet 96 Red system (Sartorius). Harvested cell media were centrifuged and cell supernatants were prepared in a 96-well plate with S-2P or S-6P standards diluted in media from 20 μg/mL to 0.3125 μg/mL ...
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bioRxiv - Cell Biology 2023Quote: ... protein was eluted and concentrated to 500 μl using a Vivaspin6 10,000 MVCO centrifugal concentrator (Sartorius, Germany). The concentrated sample was subjected to size exclusion chromatography using an Äkta FPLC UPC-900 liquid chromatography system (GE Healthcare ...
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bioRxiv - Biochemistry 2023Quote: ... The mixture of peptides was separated from large proteins by centrifugation on Vivaspin 500 concentrators (Sartorius, Germany) with a PES membrane resistant to high concentrations of salts and detergents (10 kDa molecular weight cut-off) ...
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bioRxiv - Microbiology 2024Quote: ... 10 % glycerol) and proteins were concentrated using vivaspin 6 columns with a 5000 Da cut off (Sartorius). To remove the His-tag ...
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bioRxiv - Molecular Biology 2021Quote: ... the clarified supernatant was loaded onto Vivaspin protein concentrator columns (molecular weight cut-off of 30 kDa; Sartorius) and centrifuged at 4,000 x g at 4 °C until the supernatant was 100-fold concentrated ...
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bioRxiv - Neuroscience 2022Quote: ... Protein was filtered with a 0.22 µm filter and concentrated using Vivaspin 15R 10K centrifugal filters (VS15RH01, Sartorius). Protein was then loaded onto a Superdex 200 column and 2 mL fractions were collected ...
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bioRxiv - Biochemistry 2022Quote: ... protein solutions were concentrated to about 12 mg ml-1 using a centricon centrifugal filter unit (Sartorius Stedim). The protein concentration was estimated by measuring the absorbance at 280 nm.