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Citations for Sartorius :
1 - 50 of 301 citations for Mouse Eukaryotic translation initiation factor 4E binding protein 3 EIF4EBP3 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: Antibody binding to Henipavirus F proteins was assessed using BLI on an Octet RED 384 (Sartorius, formerly ForteBio) with Forte Bio Kinetics Buffer ...
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bioRxiv - Microbiology 2022Quote: ... The supernatant was sterile-filtered using 0.2µm pore size syringe filters with low protein binding (Sartorius, ref.:16534-5). The obtained FW was stored at -80°C until further analysis.
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bioRxiv - Neuroscience 2023Quote: Bio-Layer Interferometry (BLI) for compound/protein direct binding was performed using the Octet RED384 system (Sartorius BioAnalytical Instruments) and biotinylated SYK-tSH2 protein (SYKA-c022 cleaved/biotinylated ...
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bioRxiv - Microbiology 2022Quote: ... Proteins were removed by ultrafiltration (3 kDa Vivaspin; Sartorius, Göttingen, Germany). ACE and ANSA hydrolysis activities were quantified in discontinuous HPLC-based assays at 30°C in lysis buffer ...
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bioRxiv - Biophysics 2024Quote: ... His-tagged dROS1 protein (construct 2-3) were loaded onto penta-His sensors (Sartorius) to a response unit of about 0.5 nm followed by quenching in 10 ng/mL biocytin (Sigma-Aldrich ...
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bioRxiv - Immunology 2022Quote: The binding kinetic was performed using Octet RED96E (Sartorius). SAX sensors were loaded with biotinylated ACE2 25μg/ml and subsequently quenched with biocytin ...
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bioRxiv - Microbiology 2023Quote: ... The proteins were spin concentrated to 5 mg / ml (3 kDa MWCO, vivaspin 20, Sartorius), aliquoted ...
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bioRxiv - Molecular Biology 2021Quote: ... The protein was concentrated to 3 mg/ml using a 50 kDa cutoff concentrator (Vivaspin, Sartorius). The purified protein was flash frozen in liquid nitrogen and stored at − 80 °C.
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bioRxiv - Biochemistry 2023Quote: BLI binding assays were performed on an Octet Red (Sartorius) instrument operated at 30℃ with shaking (1000 rpm) ...
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bioRxiv - Biochemistry 2024Quote: BLI binding assays were performed on an Octet Red (Sartorius) instrument operated at 30°C with shaking (1000 rpm) ...
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bioRxiv - Bioengineering 2022Quote: ... Binding reactions were performed in 1X kinetic buffer (Sartorius, 18-1105) consisting of 20 mM phosphate buffer ...
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bioRxiv - Synthetic Biology 2023Quote: Biolayer interferometry binding data were collected on an Octet R8 (Sartorius) and processed using the instrument’s integrated software ...
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bioRxiv - Molecular Biology 2023Quote: ... The labeled tip was then dipped in 1x binding buffer (Sartorius) for 60 seconds to remove excess ligand and achieve baseline ...
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bioRxiv - Immunology 2023Quote: Binding by BLI was performed using an Octet K2 system (Sartorius) using anti-human IgG-Fc biosensors (AHC ...
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bioRxiv - Biochemistry 2023Quote: Binding assays were carried out on an Octet RED96 instrument (Sartorius) using a previously established protocol (He et al. ...
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bioRxiv - Molecular Biology 2023Quote: The MultiCyt® QBeads® PlexScreen Secreted Protein Assay Kit (Sartorius) was used to quantify the level of tumor necrosis factor alpha (TNF-a ...
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bioRxiv - Biophysics 2022Quote: ... The binding kinetics were analyzed using the Octet DataAnalysis 10.0 software (Sartorius) to deduce the kinetic parameters ...
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bioRxiv - Synthetic Biology 2023Quote: ... IgGs were assessed for binding to biotinylated antigens using streptavidin biosensors (Sartorius/ForteBio). Antigen was loaded at a concentration of 200nM ...
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bioRxiv - Plant Biology 2019Quote: ... The protein without His-Tag was concentrated using a Vivaspin 20 Ultrafiltration Unit (3 kDa molecular weight cutoff, Sartorius, www.sartorius.de). An appropriate volume was applied to a 1 ml loop of a gel filtration column (Superdex75 10/300 GL ...
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bioRxiv - Immunology 2021Quote: ... S2K146 and respective UCA Ab were prepared at 3 μg/ml and immobilized on pre-hydrated protein-A biosensors (Sartorius) for 75 sec ...
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bioRxiv - Biochemistry 2022Quote: Analysis of binding kinetics was done at 23 °C on Octet K2 (Sartorius AG). A template T7 with a biotin at the 5’-end annealed to the primer P2 (Table 1 ...
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bioRxiv - Immunology 2022Quote: Binding kinetics were measured using an Octet RED96 Biolayer Interferometer (Sartorius ForteBio, Freemont, CA)30 ...
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bioRxiv - Cell Biology 2023Quote: Binding kinetics analysis of Drp1 Nbs was performed using the Octet RED96e system (Sartorius) according to the manufacturer’s recommendations ...
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bioRxiv - Biochemistry 2024Quote: Analysis of binding kinetics was done at 23 °C using Octet K2 (Sartorius AG), as previously described (61) ...
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bioRxiv - Biochemistry 2020Quote: ... Protein containing fractions were pooled and concentrated to 5 mg/ml in a Vivaspin 500 (3 kDa) centrifugal concentrator (Sartorius AG), after which they were aliquoted ...
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bioRxiv - Biochemistry 2022Quote: Analysis of binding kinetics was done at 23 ºC on an Octet K2 (Sartorius AG). This device uses Bio-Layer Interferometry technology to monitor molecular interactions in real time ...
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bioRxiv - Biochemistry 2020Quote: ... followed by concentrating and buffer exchange into IMAC binding buffer using Vivaflow 100 system (Sartorius). The resulting protein solution was purified by Histrap column (GE Healthcare ...
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bioRxiv - Immunology 2023Quote: ... BLI ligand titration and binding kinetics assays were performed on an Octet Red96e system (Sartorius) at 30°C with an orbital shake speed of 1000 rpm ...
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bioRxiv - Immunology 2023Quote: ... BLI binding experiments were performed with Anti Human IgG Fc (AHC) biosensors (Sartorius 18-5060). Using Octet buffer (PBS with 0.1% Tween20) ...
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bioRxiv - Immunology 2024Quote: ... Binding was read by flow cytometry on an IntelliCyte High Throughput Flow Cytometry (IntelliCyte/Sartorius).
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bioRxiv - Immunology 2022Quote: The binding affinity of obtained antibodies to RBD was examined by the Blitz system (Sartorius Japan) using protein A-coated biosensors ...
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bioRxiv - Cell Biology 2021Quote: Analysis of binding kinetics of Miro1-specific Nbs was performed using the Octet RED96e system (Sartorius) according to the manufacturer’s recommendations ...
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bioRxiv - Biochemistry 2022Quote: Competition binding assays were performed by a premix method with streptavidin bio-sensor (SA sensor, Sartorius). Firstly ...
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bioRxiv - Synthetic Biology 2022Quote: The binding kinetics of antibodies to RBD was measured by BLI on an Octet-R2 (Sartorius). The his-tagged RBD variants were loaded onto Ni-NTA biosensors from 10 ug\ml solution in kinetic buffer (PBS ...
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bioRxiv - Synthetic Biology 2022Quote: ... IgGs and Fabs were assessed for binding to biotinylated antigens using streptavidin (SA) biosensors (Sartorius/ForteBio) or to unbiotinylated ...
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bioRxiv - Microbiology 2023Quote: We performed human plasma and avidity variant Abs’ binding using an Octet Red384 biolayer interferometer (Sartorius). We completed all the steps in 0.05% BSA and 0.002% Tween20 ...
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bioRxiv - Immunology 2023Quote: Analysis of binding kinetics of hSIRPα specific Nbs was performed using the Octet RED96e system (Sartorius) as per the manufacturer’s recommendations ...
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bioRxiv - Molecular Biology 2023Quote: ... The IgG loaded biosensor tips then were submerged in binding buffer (kinetics buffer (Sartorius #18-1105) in PBS ...
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bioRxiv - Cancer Biology 2023Quote: ... the FcRn binding assay was measured in a biolayer interferometry assay on an Octet RED96 system (Sartorius). Streptavidin-coated biosensors were loaded with purified biotinylated human FcRn in pH 6.0 assay buffer (100mM sodium phosphate ...
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bioRxiv - Biophysics 2024Quote: ... The acquired raw data for the binding of anti-TcPOP mAbs with TcPOP were processed and globally fit to a 1:1 binding model with Octet Analysis Studio 12.2.2.26 Software (Sartorius). The binding kinetics measurements were carried out in three replicates ...
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bioRxiv - Bioengineering 2019Quote: ... 3 kDa MWCO concentrator (Sartorius), and the protein concentration was estimated using a BCA assay.
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bioRxiv - Bioengineering 2019Quote: ... 3 kDa MWCO concentrator (Sartorius). Protein concentrations were estimated by BCA assay.
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bioRxiv - Microbiology 2022Quote: Binding kinetics measurements were characterized using biolayer interferometry (BLI) measurements on an Octet QKe instrument (Sartorius, Göttingen, Germany), as previously described 66 ...
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bioRxiv - Cancer Biology 2023Quote: ... The binding affinity between HER3-ECD and LRG1 or neuregulins was measured by the Octet RED96 system (Sartorius), at 25 °C in 1X PBS buffer ...
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bioRxiv - Immunology 2024Quote: The binding kinetics of ZBP1-Zα1Zα2 with SARS-CoV-2 RNAs were evaluated using the Octet RED96 instrument (Sartorius). First ...
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bioRxiv - Immunology 2021Quote: ... biotinylated S1 binding mAbs at 25 µg/mL were first loaded on High Precision Streptavidin SAX biosensors (Sartorius Corporation) for 10 sec ...
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bioRxiv - Biochemistry 2022Quote: ... IgGs produced from the scFvs from the above sort were assessed for binding using streptavidin (SA) biosensors (Sartorius/ForteBio) loaded to a threshold of 0.8nm of SARS-CoV- 2 ...
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bioRxiv - Immunology 2022Quote: ... Non-specific binding was assessed using a control anti-influenza haemagglutinin mAb (CH65) for reference subtraction and the binding curves were analyzed using the ForteBio Data Analysis Software 10.0 (Sartorius). Binding responses (nm ...
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bioRxiv - Immunology 2023Quote: The binding affinity of recombinant antibodies to antigens was estimated by bio-layer interferometry (Octet® QK, ForteBio/Sartorius). Antibodies were loaded at 10-20 nM onto anti-human IgG Fc capture or anti-Mouse IgG Fc capture biosensors ...
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bioRxiv - Bioengineering 2023Quote: ... Analysis and kinetic curve fitting (assuming a 1:1 binding model) were conducted using Octet Data Analysis HT software version 7.1 (Sartorius). Normalized equilibrium binding curves were obtained by plotting the response value at the end of the association phase for each sample dilution and normalizing to the maximum value ...