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Citations for Sartorius :
51 - 100 of 110 citations for Mouse Anti Hepatitis B Virus Core Antibody M413 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... IncuCyte® Annexin V Green Reagent was added directly in the medium at the dilution 1:200 and B cells were imaged every hour for 3.5 days using IncuCyte® Live-Cell Analysis System (Sartorius).
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bioRxiv - Microbiology 2023Quote: ... at a shaking speed of 150 rpm and used to start the batch phase in a lab-scale stirred bioreactor (Biostat B-Twin, Sartorius) total volume ...
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bioRxiv - Biochemistry 2022Quote: The bioreactor cell culture experiments were conducted in a 3L glass bioreactor using Biostat B-DCU controller (Sartorius, Göttingen, Germany) with a working volume of 1.75L ...
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bioRxiv - Plant Biology 2024Quote: ... FLVB and FLVA/B were analyzed by SDS-page and then concentrated by ultra filtration (Sartorius, Vivaspin, 10 kDa cutoff). Analysis of different purification steps and quality of protein purifications was also assessed by Immunoblot analysis with custom-made α-FLVA ...
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bioRxiv - Immunology 2021Quote: ... 7 L of Sf9 cell culture at a density of 2 × 106 cells/mL were infected with the baculovirus at a MOI of 3 using a Biostat B plus bioreactor (Sartorius, Germany). The following conditions were maintained during the culture period ...
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bioRxiv - Microbiology 2020Quote: Batch and fed-batch bioreactor experiments were performed in 2 L bench top glass fermentors (Biostat B, Sartorius Stedim, Göttingen, Germany) equipped with two 6-blade Rushton impellers ...
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bioRxiv - Microbiology 2019Quote: The experimental platform is an improved version of a previous on-line flow cytometry platform [28][12] and actually comprises three modules (Figure 1): (a) a conventional culture device (Biostat B-Twin, Sartorius, 2 L), a physical interface for sampling and dilution comprising peristaltic pumps and a mixing chamber (c ...
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bioRxiv - Physiology 2019Quote: ... were inoculated with 2 mL of stock culture and grown at 30°C and at 200 rpm for 8 h in a B Braun Certomat BS-1 incubator (Sartorius, Melsungen, Germany). Bioreactor batch cultures were started by transferring 400 mL of preculture to a bioreactor containing 4.6 L of medium ...
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bioRxiv - Biochemistry 2023Quote: The perfusion cell culture experiments were conducted in a 5 L glass bioreactor using a Biostat B-DCU controller (Sartorius, Göttingen, Germany) with a working volume of 2 L ...
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bioRxiv - Microbiology 2019Quote: ... Cultures in chemostat and segregostat mode have been performed in lab-scale stirred bioreactor (Biostat B-Twin, Sartorius. Total volume: 2L; working volume: 1L). For the batch phase ...
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bioRxiv - Systems Biology 2023Quote: ... Cells were washed once with warm (37°C) PBS and then lifted from wells using Trypsin EDTA solution B (Sartorius, Cat# 03-052-1B) (5mins ...
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bioRxiv - Bioengineering 2023Quote: ... Mouse Fc-capture biosensor tips were purchased from Sartorius. Chicken plasma (cat # ...
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bioRxiv - Cell Biology 2020Quote: A mouse brain was homogenized using a dounce tissue grinder (Sartorius) in 1ml MES buffer (100mM MES ...
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bioRxiv - Immunology 2022Quote: ... Anti-hIgG Fc capture (AHC) or anti-mIgG Fc capture sensors (Sartorius) were functionalized with monoclonal antibodies ...
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bioRxiv - Immunology 2021Quote: ... Anti-penta-HIS biosensors (Sartorius) were immersed in kinetics buffer (PBS + 0.5% BSA + 0.05% Tween-20 ...
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bioRxiv - Immunology 2022Quote: ... Anti-penta-HIS biosensors (Sartorius) were immersed in kinetics buffer (PBS + 0.5% BSA + 0.05% Tween-20 ...
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bioRxiv - Biochemistry 2022Quote: Anti-His-tag (HIS1K) biosensors (Sartorius) were incubated in BLI buffer (0.002% v/v Tween-20 in 1× PBS ...
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bioRxiv - Biochemistry 2023Quote: ... Anti-GST biosensors (Sartorius #18-5096) were used to immobilise GST-Rgl2RA (provided as a 0.7 µM solution in the binding reservoir well ...
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bioRxiv - Cancer Biology 2024Quote: Internalization was assessed using the Incucyte Fabfluor-pH Antibody labeling reagent (Sartorius, Germany), following the manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: ... Anti-Human Fc sensor tips (ForteBio, now Sartorius) were loaded with 200 nM mAb at a threshold of 0.8nm and then submerged into wells containing 100 nM (protomer/monomer concentration ...
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bioRxiv - Biochemistry 2023Quote: ... Anti-Human Fc sensor tips (ForteBio, now Sartorius) were loaded with 200 nM mAb or FcACE2 to a threshold of 0.8 nm and then submerged into wells containing 100 nM (protomer/monomer concentration ...
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bioRxiv - Neuroscience 2023Quote: ... nasal lavage fluid harvested from the mouse model was filtered through a 0.2-µm syringe filter (Sartorius, Goettingen, Germany) to remove aggregates ...
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bioRxiv - Microbiology 2020Quote: ... The purified antibodies were buffer exchanged to PBS using 100 kDa VivaSpin20 columns (Sartorius). The IgG concentration was determined on the NanoDrop 2000 and the antibodies were stored at 4°C until further analyses.
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bioRxiv - Molecular Biology 2024Quote: ... Anti-human IgG Fc capture (AHC) biosensor tips (Sartorius) were equilibrated for ten minutes in a 1:4 mixture of conditioned Freestyle 293 medium and 1X HBS EP+ to match the diluted media ...
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bioRxiv - Immunology 2022Quote: BLI experiments on the antagonistic antibodies were performed using an Octet Red 96 machine (Sartorius) in kinetics buffer (PBS ...
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bioRxiv - Synthetic Biology 2022Quote: ... His-tagged antigens using Anti-Penta-HIS biosensors (Sartorius/ForteBio). Antigen was loaded to a threshold of 1 nm shift ...
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bioRxiv - Immunology 2022Quote: The binding affinity of obtained antibodies to RBD was examined by the Blitz system (Sartorius Japan) using protein A-coated biosensors ...
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bioRxiv - Synthetic Biology 2022Quote: The binding kinetics of antibodies to RBD was measured by BLI on an Octet-R2 (Sartorius). The his-tagged RBD variants were loaded onto Ni-NTA biosensors from 10 ug\ml solution in kinetic buffer (PBS ...
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bioRxiv - Immunology 2024Quote: ... GST-c-SLFN11 was immobilized onto Anti-GST Biosensor tips (Sartorius) in BLI buffer by dipping the biosensors into wells containing GST-c-SLFN11 for 300 s ...
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bioRxiv - Immunology 2020Quote: ... The antibodies were buffer-exchanged into PBS and concentrated using 10,000 MWCO Vivaspin centrifugal spin columns (Sartorius).
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bioRxiv - Immunology 2024Quote: ... Supernatants were quantitated for antibody concentrations by bio-layer interferometry (BLI) on an Octet Red 96 (Sartorius) using protein A biosensors and a human IgG1 isotype control antibody as a standard ...
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bioRxiv - Immunology 2024Quote: ... The antibodies were buffer-exchanged into PBS and concentrated using 10,000 MWCO Vivaspin centrifugal spin columns (Sartorius).
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bioRxiv - Immunology 2021Quote: ... IgGs were immobilized on Anti-hIgG Fc Capture (AHC) biosensors (Sartorius Corporation) and a concentration series of 200 ...
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bioRxiv - Bioengineering 2022Quote: ... and was captured by anti-Penta-His (HIS1K) sensors (Sartorius, 18-5120). The primary antibody was diluted to 150nM with a running buffer in wells ...
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bioRxiv - Synthetic Biology 2023Quote: ... and captured on Anti-hIgG Fc Capture (AHC) Biosensors (Sartorius 18-5060). Capture was recorded for 40 seconds ...
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bioRxiv - Molecular Biology 2023Quote: Antibody binding to Henipavirus F proteins was assessed using BLI on an Octet RED 384 (Sartorius, formerly ForteBio) with Forte Bio Kinetics Buffer ...
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bioRxiv - Immunology 2024Quote: ... Antibodies were diluted to a final concentration of 20 ug/mL before being immobilized on ProteinA tips (Sartorius) for 600 s ...
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bioRxiv - Immunology 2021Quote: ... S2-His-tag was immobilized on Anti-Penta-His (HIS1K) biosensors (Sartorius Corporation), and a concentration series of S2 binding mAb Fab at 200 ...
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bioRxiv - Evolutionary Biology 2022Quote: ... IgG was loaded onto Anti-Human Fc Capture (AHC) biosensors (Sartorius, #18-5060). To reduce the avidity effect ...
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bioRxiv - Immunology 2021Quote: Affinity measurements for VHH-huFc antibodies were performed using biolayer interferometry (BLI) using an Octet 384 Red system (Sartorius). Measurements were conducted in 10 mM phosphate (pH 7.4) ...
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bioRxiv - Immunology 2023Quote: The binding affinity of recombinant antibodies to antigens was estimated by bio-layer interferometry (Octet® QK, ForteBio/Sartorius). Antibodies were loaded at 10-20 nM onto anti-human IgG Fc capture or anti-Mouse IgG Fc capture biosensors ...
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bioRxiv - Immunology 2024Quote: Analysis of serum antibodies binding to PNP scaffolds by biolayer interferometry (BLI) was performed on an Octet R4 (Sartorius) at 30 °C in kinetics buffer (PBS ...
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bioRxiv - Immunology 2022Quote: Binding kinetics of WS6 IgG and related S2-stem helix antibodies to diverse CoV 2P-stabilized spike proteins (S2Ps) were measured by biolayer interferometry using an Octet HTX instrument (Sartorius). All assays were performed in tilted 384-well plates (Geiger Bio-one ...
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bioRxiv - Biochemistry 2024Quote: ... Anti-FLAG M2 was immobilized on protein G biosensors (Sartorius Cat. No. 18-5082) at 5 µg/mL (in binding buffer ...
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bioRxiv - Immunology 2023Quote: ... BLI binding experiments were performed with Anti Human IgG Fc (AHC) biosensors (Sartorius 18-5060). Using Octet buffer (PBS with 0.1% Tween20) ...
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bioRxiv - Bioengineering 2022Quote: ... The five fermentation experiments to produce a monoclonal antibody were all conducted in controlled automated miniaturized bioreactor (ambr250) systems (Sartorius AG, Germany) for 14 days ...
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bioRxiv - Microbiology 2022Quote: The affinity between each antibody and spike protein was measured by bio-layer interferometry (BLI) with a BLItz Biolayer Interferometer System (Sartorius, Goettingen, Germany).
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bioRxiv - Synthetic Biology 2021Quote: ... The required amount of antibody was diluted in PBS (5ml) and applied to a Vivaspin 6 concentrator (Sartorius; VS0601; MWCO: 10kDa; 5ml volume). After centrifugation (4000 x g ...
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bioRxiv - Cancer Biology 2023Quote: ... of PD-L1 protein/PD-L1 antibody was determined by Octet Biolayer interferometry (BLI) using the Octet RED384 system (Sartorius, Bohemia, NY, USA). Briefly ...
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bioRxiv - Immunology 2021Quote: ... His-tagged SARS-CoV-2 RBD was prepared at 8 μg/ml in Kinetics buffer (PBS 0.01% BSA) and loaded on pre-hydrated anti-penta-HIS biosensors (Sartorius) for 2.5 min ...