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Citations for Sartorius :
251 - 288 of 288 citations for Methylphosphonic Acid 13C 99%; Methyl D3 98% 100 Ug Ml In Methanol since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2023Quote: ... The flow-through volume containing the untagged protein was concentrated to 10 mg/mL using Vivacon500 filters (50 kDa, Sartorius) for further purification using a Superdex 200 Increase 5/150 GL column equilibrated in 50 mM Tris-HCl pH 7.0 ...
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bioRxiv - Microbiology 2024Quote: ... the fractions obtained by affinity chromatography were pooled and concentrated using conical membrane ultrafiltration tubes (Vivaspin 6 mL 10 kDa MWCO, Sartorius) to 7 mg/mL (2 mL) ...
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bioRxiv - Immunology 2024Quote: ... Biotinylated OspA (5 µg/mL) in PBS containing 2% w/v BSA was captured onto streptavidin biosensors (#18-5019, Sartorius) for 5 min ...
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bioRxiv - Immunology 2021Quote: ... recombinant SARS-CoV-2 S2-His-tag at 10 µg/mL was loaded on Anti-Penta-HIS (HIS1K) biosensors (Sartorius Corporation) for 1 min ...
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bioRxiv - Developmental Biology 2020Quote: ... and LSECs were supplemented with 10µg/ml FSA-FITC (prepared as described in (47)) and 1:1000 IncuCyte® NucLight Rapid Red Reagent (cat. 4717, Sartorius). Cells were imaged hourly over a period of 24 hours using the IncuCyte Cell analysis system (Essen Bioscience) ...
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bioRxiv - Biochemistry 2021Quote: A stock solution of sfGFP-ACE2 was diluted to at 10 μg/ml for immobilization onto High Precision Streptavidin (SAX) biosensors (Sartorius, Germany) in assay buffer (50 mM Tris-HCl ...
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bioRxiv - Biochemistry 2020Quote: ... Protein containing fractions were pooled and concentrated to 5 mg/ml in a Vivaspin 500 (3 kDa) centrifugal concentrator (Sartorius AG), after which they were aliquoted ...
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bioRxiv - Plant Biology 2020Quote: ... Material for analyses was obtained from seeds pre-cooled for 15 min in 5-mL Teflon capsules (Sartorius GmbH, Göttingen, Germany), containing a 10 mm-diameter agate bead ...
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bioRxiv - Biochemistry 2022Quote: ... The clear supernatant was microfiltered (0.22 µm, PES) and concentrated to 50 mL using a Sartorius Vivacell 250 PES Centrifugal Concentrator (Sartorius, Göttingen, Germany) with a MWCO of 100 kDa and a pressure of 4 bar ...
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bioRxiv - Immunology 2021Quote: ... 7 L of Sf9 cell culture at a density of 2 × 106 cells/mL were infected with the baculovirus at a MOI of 3 using a Biostat B plus bioreactor (Sartorius, Germany). The following conditions were maintained during the culture period ...
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bioRxiv - Synthetic Biology 2020Quote: Strains were pre-cultured as above in sterile 50 mL Falcon tubes prior to inoculation at an OD600 of 0.02 in ambr® 250 (Sartorius Stedim) microbial bioreactors with 140 mL of medium ...
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bioRxiv - Biochemistry 2020Quote: ... The clarified supernatant was concentrated by ultrafiltration to approximately 70 ml using one 200 cm2 10 kDa MWCO PES tangential flow filtration cassette (Sartorius, Germany) and the ultrafiltration reservoir (Sartorius) ...
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Maize AFP1 confers antifungal activity by inhibiting chitin deacetylases from a broad range of fungibioRxiv - Microbiology 2021Quote: ... A total 75 ml culture supernatant was concentrated and exchanged with buffer W using 10 kD cutoff centrifugal filters (Sartorius, Germany). A 50 µl of Strep-Tactin®XT agarose beads were added to the concentrated sample in a final volume of 1 ml containing 1x protease inhibitor cocktail and incubated overnight at 4 ℃ ...
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bioRxiv - Bioengineering 2023Quote: ... COS7 cells at a concentration of 60,000 cells/ml in Dulbecco’s Modified Eagle Medium (DMEM) with 1g/l D-glucose (Sartorius, 01-050-1A), supplemented with fetal bovine serum (Biological Industries ...
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bioRxiv - Molecular Biology 2023Quote: ... The cells were resuspended in 2 mL 50 mM Tris buffer (pH 7.6) and lysed by using a dismembrator (Sartorius, Göttingen, Germany) at 1,600 rpm for 3 min ...
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bioRxiv - Molecular Biology 2023Quote: ... The cells were resuspended in 10 mL Tris buffer (50mM, pH 7.6) and afterwards lysed by using a dismembrator (Sartorius, Göttingen, Germany) at 1,600 rpm for 3 min ...
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bioRxiv - Plant Biology 2023Quote: ... 250 mM imidazole) and concentrated to 1-2 mL by ultrafiltration using a Vivaspin® Turbo Centrifugal Concentrator (Sartorius, United Kingdom). To remove imidazole ...
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bioRxiv - Microbiology 2023Quote: ... were dialyzed against 20 mM Tris-HCl (pH 8.0) and concentrated to 10-15 mg/ml using Vivaspin Ultra concentrators (Sartorius, Göttingen, Germany). Purity of the proteins was analyzed after sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE ...
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bioRxiv - Plant Biology 2024Quote: ... Fractions were pooled and concentrated to a final concentration of 2 mg/ml using a centrifugal filter device (Sartorius, Göttingen, Germany) with a molecular weight cut-off of 50 kDa.
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bioRxiv - Biochemistry 2024Quote: ... adding 3 ml of ice-cold Tris buffer and by filtrating the PLs through 0.45 μm pore-size membrane filters (Sartorius Stedim Biotech). Filters were washed twice with 3 ml of Tris buffer and allowed to dry ...
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bioRxiv - Microbiology 2021Quote: ... The cell-free viral fraction was concentrated to about 50 mL with tangential flow filtration (50R VivaFlow, Sartorius Lab instruments, Goettingen, Germany) and used as inoculum in a multi-step viral enrichment experiment followed by Dilution-to-Extinction purification as described previously (15) ...
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bioRxiv - Biochemistry 2020Quote: ... The peak fractions containing ANT(9) were pooled and concentrated to 6 mg/ml using a Vivaspin 10 kDa cut-off concentrator (Sartorius AG, Germany).
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bioRxiv - Cancer Biology 2020Quote: ... were homogenized in liquid nitrogen pre-cooled 4 ml PTFE-vials with one 8 mm stainless steel bead using a Micro-Dismembranator (Sartorius AG, Göttingen) at 2600 RPM for two minutes ...
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bioRxiv - Plant Biology 2022Quote: ... pastoris cells were pelleted by centrifugation at 3800 g at 4°C for 50 min and the supernatant was concentrated to 200 ml using a Vivaflow 200 Cross Flow Cassette (5000NWCO; Sartorius, Göttingen, Germany) at 4°C for approximately 20 h ...
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bioRxiv - Microbiology 2020Quote: ... filtered at 0.22 μm and concentrated to 1 mL using a Vivaspin turbo ultrafiltration units (10 kDa MWCO, Sartorius AG, Goettingen, Germany). Samples were further concentrated to 50 μL after five successive washes using Tris 50 mM ...
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bioRxiv - Synthetic Biology 2022Quote: ... Cell dry weight (CDW) measurements were performed by filtrating 1 mL of cell culture through a weighed 0.45 μm filter membrane (Sartorius Biolab, Gottingen, Germany) and weighting the filter after microwaving it for 15 min and letting it dry in a desiccator for 24h.
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bioRxiv - Immunology 2022Quote: Biotinylated OspCA (3 μg/mL) in buffer (PBS containing 2% w/v BSA) was captured onto streptavidin biosensors (#18-5019, Sartorius, Goettingen, Germany) for 5 min ...
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bioRxiv - Cell Biology 2023Quote: ... HoxB8 cells were seeded in media with or without 1 µg/ml Doxycycline and 1 µg/ml PI and imaged every 2h in an Incucyte S3 microscope using the 10x objective (Sartorius, Göttingen, Germany). The analysis function of the system was used to calculate the red object count (ROC) ...
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bioRxiv - Molecular Biology 2023Quote: ... The peak fractions were concentrated to 5-10 mg/ml with a 30-KDa cutoff Vivaspin Turbo 15 (Sartorius AG, Göttingen, Germany), flash-frozen in liquid nitrogen ...
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bioRxiv - Genetics 2021Quote: ... according to manufacturer’s protocol and cultured at 1×105 cells/mL in CellGenix GMP SCGM serum-free base media (Sartorius CellGenix GmbH, Freiburg, Germany) supplemented with stem cell factor (SCF)(100ng/mL) ...
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bioRxiv - Genomics 2022Quote: ... according to manufacturer’s protocol and cultured at 1×105 cells/mL in CellGenix GMP SCGM serum-free base media (Sartorius CellGenix GmbH, Freiburg, Germany) supplemented with stem cell factor (SCF)(100ng/mL) ...
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bioRxiv - Plant Biology 2019Quote: ... These filtrates were ultra-filtered at 4°C through 20-ml Vivaspin micro-concentrators (10,000 molecular weight cut off, Sartorius Stedim, Biotech GmbH, Germany). Retained protein suspensions were used for protein content and enzyme assays ...
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bioRxiv - Biochemistry 2020Quote: ... we concentrated the proteins from the supernate by membrane centrifugation using 5 K molecular weight cutoff Vivaspin units of 6 mL (Sartorius Stedim, Goettingen, Germany). The retentate (~300 μL ...
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bioRxiv - Microbiology 2023Quote: ... Fc-tagged RBD proteins (20 μg/mL) were captured on the protein A biosensor followed by 30 s of baseline run in 1x kinetics buffer (Sartorius Cat#: 18-1105). The biosensors were blocked with a control Fc protein (Jackson ImmunoResearch Cat# ...
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bioRxiv - Bioengineering 2023Quote: ... was washed three times in DI water and concentrated to a final volume of 1 mL using 3 kDa MWCO ultrafiltration tubes (Sartorius Stedim Biotech, Germany). The tubes were spun at 4000 rpm for 40 minutes ...
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bioRxiv - Biophysics 2020Quote: ... The flow-through of the column was concentrated (Vivaspin®, mwco 10 or 30 kDa for single SBD or tandem mutants, Sartorius; ~ 5 mg/ml), dialyzed in buffer A supplemented with 50 % glycerol ...
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bioRxiv - Evolutionary Biology 2019Quote: ... The fractions containing MGE protein were pooled and concentrated to 1 mL by using a centrifugal concentrator (Sartorius, 10 kDa MWCO, Vivaspin turbo 15). The recombinant proteins were further purified by ion exchange and gel filtration chromatography with ÄKTAprime plus system ...
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bioRxiv - Evolutionary Biology 2019Quote: ... The fractions containing MGE protein were pooled and concentrated to 1 mL by using a centrifugal concentrator (Sartorius, 10 kDa MWCO, Vivaspin turbo 15). For gel filtration ...