Labshake search
Citations for Sartorius :
51 - 100 of 288 citations for Insulin like growth factor binding protein 6 IGFBP 6 Mouse HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biochemistry 2021Quote: ... Purified V-ATPase in buffer containing LMNG was concentrated to 1.9 mg/mL with a 100 kDa MWCO VivaSpin 6 centrifugal concentrator at 2000 ×g for 15 min (Sartorius), followed by a VivaSpin 500 concentrator at 12,000 ×g for 2 min.
-
bioRxiv - Biophysics 2023Quote: ... Fractions containing Ub were concentrated and buffer exchanged into pH 6.8 buffer (see above) using Vivaspin 6 centrifugal concentrators with a molecular weight cutoff (MWCO) of 5000 Da (Sartorius). Purified proteins were frozen at −80°C.
-
bioRxiv - Microbiology 2023Quote: ... whole well images were taken at 6 h intervals using an Incucyte Zoom S3 live cell imaging system (Sartorius).
-
bioRxiv - Microbiology 2023Quote: ... Total green cell count was measured every 6 h using an Incucyte Zoom S3 live cell imaging system (Sartorius) and normalised for confluency variation ...
-
bioRxiv - Biophysics 2023Quote: ... The eluted protein was then concentrated to the approximate concentration of 10 mg/ml using a Vivaspin 6 3,000 MWCO PES (Sartorius) membrane ultrafiltration tube.
-
bioRxiv - Bioengineering 2024Quote: ... and arginine (pH 6) to a final concentration of ≥200 mg/mL using the Ambr Crossflow system (Sartorius, Germany).
-
bioRxiv - Biochemistry 2022Quote: ... The fractions were checked via SDS-PAGE and the cleanest Chz1-containing fraction was concentrated using a 10 kDa cutoff Vivaspin 6 concentrator (Sartorius) at 3,000 g at 4°C ...
-
bioRxiv - Molecular Biology 2021Quote: ... The nuclear suspension was centrifugated for 30 min at 15 000 g and supernatant was concentrated using Vivaspin 6 (10 kDa MWCO) centrifugal concentrator (Sartorius). Aliquots of concentrated nuclear extracts were frozen in liquid nitrogen and stored at -80 °C.
-
bioRxiv - Systems Biology 2020Quote: 2 mL of collected spent media was loaded in Vivaspin 6™ Centrifugal Concentrator 10,000 MWCO PES (Sartorius AG, Germany) and centrifuged at 4000 RPM for 13 minutes to concentrate samples ...
-
bioRxiv - Neuroscience 2022Quote: ... The pooled fractions of the NOTCH3 EGF1-5 WT and R133C were concentrated to 1 mg/mL in a concentration column (Vivaspin 6 10 KDa MWCO, Sartorius) according to the manufacturer’s instructions ...
-
bioRxiv - Genetics 2023Quote: ... and cells were imaged live every 6 hours using the IncuCyte® ZOO system for automated live cell analysis (Sartorius). Wound closure was analysed by measuring the spatial cell density in the wound area relative to the spatial cell density outside of the wound expressed as the Relative Wound Density (% ...
-
bioRxiv - Biochemistry 2023Quote: ... The flow through was collected and concentrated to 6-10 mg/ml using a 100 kDa molecular weight (MW) cutoff centrifugal concentrator (Sartorius) and loaded onto a Superdex 200 Increase 10/300 GL column equilibrated with 20 mM Tris-HCl (pH 7.5) ...
-
bioRxiv - Biophysics 2023Quote: Nanodisc samples were concentrated to ∼200 μM in 280 μL in a Vivaspin-6 concentrator with a 30 kDa MWCO (Sartorius). 20 μL D2O was added and gently mixed into the sample ...
-
bioRxiv - Neuroscience 2023Quote: ... Eluent from the first two elution fractions after the elimination of dead volume were concentrated using Vivaspin 6 Centrifugal Concentrators (Sartorius). Imidazole was removed from the concentrated eluent using PD MiniTrap Sample Preparation Columns (Cytiva ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... Eluent from the first two elution fractions after the elimination of dead volume were concentrated using Vivaspin 6 Centrifugal Concentrators (Sartorius). Imidazole was removed from the concentrated eluent using PD MiniTrap Sample Preparation Columns (Cytiva ...
-
bioRxiv - Microbiology 2024Quote: ... the fractions obtained by affinity chromatography were pooled and concentrated using conical membrane ultrafiltration tubes (Vivaspin 6 mL 10 kDa MWCO, Sartorius) to 7 mg/mL (2 mL) ...
-
bioRxiv - Immunology 2024Quote: ... Plates were imaged every 4-6 hours for 3-7 days using the IncuCyte® Live-Cell Analysis System (Sartorius). 5 images per well at 10x zoom were collected at each time point ...
-
bioRxiv - Microbiology 2021Quote: ... Fractions of IMAC eluate containing Pdr5 were pooled and concentrated using a Vivaspin 6 centrifugal concentrator unit (Sartorius AG, Göttingen, Germany) with a molecular weight cut-off (MWCO ...
-
bioRxiv - Microbiology 2023Quote: ... A sample of 6 liters of air per min was collected during the <10 min exposure on the 47 mm gelatin filter (Sartorius, Germany). Post exposure ...
-
bioRxiv - Cancer Biology 2021Quote: ... Time-lapse phase-contrast and fluorescent channel images are obtained at 6 hour intervals for up to 24 spheroids for each initial condition using an Incucyte S3 (Sartorius, Goettingen, Germany).
-
bioRxiv - Biochemistry 2020Quote: ... The peak fractions containing ANT(9) were pooled and concentrated to 6 mg/ml using a Vivaspin 10 kDa cut-off concentrator (Sartorius AG, Germany).
-
bioRxiv - Immunology 2021Quote: ... recombinant SARS-CoV-2 S2-His-tag at 10 µg/mL was loaded on Anti-Penta-HIS (HIS1K) biosensors (Sartorius Corporation) for 1 min ...
-
bioRxiv - Immunology 2022Quote: The binding kinetic was performed using Octet RED96E (Sartorius). SAX sensors were loaded with biotinylated ACE2 25μg/ml and subsequently quenched with biocytin ...
-
bioRxiv - Cancer Biology 2022Quote: ... growth was tracked on Incucyte S3 (Sartorius) and CellTiterGlo (CTG ...
-
bioRxiv - Synthetic Biology 2021Quote: ... The required amount of antibody was diluted in PBS (5ml) and applied to a Vivaspin 6 concentrator (Sartorius; VS0601; MWCO: 10kDa; 5ml volume). After centrifugation (4000 x g ...
-
bioRxiv - Biochemistry 2023Quote: BLI binding assays were performed on an Octet Red (Sartorius) instrument operated at 30℃ with shaking (1000 rpm) ...
-
bioRxiv - Biochemistry 2024Quote: BLI binding assays were performed on an Octet Red (Sartorius) instrument operated at 30°C with shaking (1000 rpm) ...
-
bioRxiv - Synthetic Biology 2021Quote: ... The required amount of tomato lectin was diluted in PBS (5ml) and applied to a Vivaspin 6 concentrator (Sartorius; VS0601; MWCO: 10kDa; 5ml volume). After centrifugation (4000 x g ...
-
bioRxiv - Bioengineering 2022Quote: ... Binding reactions were performed in 1X kinetic buffer (Sartorius, 18-1105) consisting of 20 mM phosphate buffer ...
-
bioRxiv - Biochemistry 2023Quote: Binding assays were carried out on an Octet RED96 instrument (Sartorius) using a previously established protocol (He et al. ...
-
bioRxiv - Molecular Biology 2023Quote: ... The labeled tip was then dipped in 1x binding buffer (Sartorius) for 60 seconds to remove excess ligand and achieve baseline ...
-
bioRxiv - Synthetic Biology 2023Quote: Biolayer interferometry binding data were collected on an Octet R8 (Sartorius) and processed using the instrument’s integrated software ...
-
bioRxiv - Immunology 2023Quote: Binding by BLI was performed using an Octet K2 system (Sartorius) using anti-human IgG-Fc biosensors (AHC ...
-
bioRxiv - Cell Biology 2023Quote: ... of either parental or mNG-modified cells were plated per well in a 6-well plate in triplicate and placed in an Incucyte system (Sartorius, Essen BioScience, Inc. Ann Arbor, MI). Four images per well (total =12 per sample ...
-
bioRxiv - Immunology 2021Quote: ... His-tagged ACE2 was immobilized onto Ni-NTA coated biosensors (Sartorius) before association of increasing concentrations of each RBD for 120 seconds followed by dissociation using HBS pH 7.4 for 360 seconds ...
-
bioRxiv - Biophysics 2022Quote: ... The binding kinetics were analyzed using the Octet DataAnalysis 10.0 software (Sartorius) to deduce the kinetic parameters ...
-
bioRxiv - Bioengineering 2022Quote: ... and was captured by anti-Penta-His (HIS1K) sensors (Sartorius, 18-5120). The primary antibody was diluted to 150nM with a running buffer in wells ...
-
bioRxiv - Synthetic Biology 2023Quote: ... IgGs were assessed for binding to biotinylated antigens using streptavidin biosensors (Sartorius/ForteBio). Antigen was loaded at a concentration of 200nM ...
-
bioRxiv - Biochemistry 2022Quote: Analysis of binding kinetics was done at 23 °C on Octet K2 (Sartorius AG). A template T7 with a biotin at the 5’-end annealed to the primer P2 (Table 1 ...
-
bioRxiv - Immunology 2022Quote: Binding kinetics were measured using an Octet RED96 Biolayer Interferometer (Sartorius ForteBio, Freemont, CA)30 ...
-
bioRxiv - Cell Biology 2023Quote: Binding kinetics analysis of Drp1 Nbs was performed using the Octet RED96e system (Sartorius) according to the manufacturer’s recommendations ...
-
bioRxiv - Biochemistry 2024Quote: Analysis of binding kinetics was done at 23 °C using Octet K2 (Sartorius AG), as previously described (61) ...
-
bioRxiv - Developmental Biology 2022Quote: Growth curves were generated from Incucyte® Live Cell Analysis System (Sartorius) 2-D images of organoids taken daily from days 6-12 ...
-
bioRxiv - Biochemistry 2022Quote: Analysis of binding kinetics was done at 23 ºC on an Octet K2 (Sartorius AG). This device uses Bio-Layer Interferometry technology to monitor molecular interactions in real time ...
-
bioRxiv - Biochemistry 2020Quote: ... followed by concentrating and buffer exchange into IMAC binding buffer using Vivaflow 100 system (Sartorius). The resulting protein solution was purified by Histrap column (GE Healthcare ...
-
bioRxiv - Immunology 2023Quote: ... BLI ligand titration and binding kinetics assays were performed on an Octet Red96e system (Sartorius) at 30°C with an orbital shake speed of 1000 rpm ...
-
bioRxiv - Immunology 2023Quote: ... BLI binding experiments were performed with Anti Human IgG Fc (AHC) biosensors (Sartorius 18-5060). Using Octet buffer (PBS with 0.1% Tween20) ...
-
bioRxiv - Immunology 2024Quote: ... Binding was read by flow cytometry on an IntelliCyte High Throughput Flow Cytometry (IntelliCyte/Sartorius).
-
bioRxiv - Cancer Biology 2023Quote: ... 3D growth was determined using IncuCyte® S3 Live-Cell Analysis system (Sartorius) over the indicated time periods.
-
bioRxiv - Immunology 2022Quote: The binding affinity of obtained antibodies to RBD was examined by the Blitz system (Sartorius Japan) using protein A-coated biosensors ...