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Citations for Sartorius :
151 - 185 of 185 citations for IL 10 Mouse since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Maize AFP1 confers antifungal activity by inhibiting chitin deacetylases from a broad range of fungibioRxiv - Microbiology 2021Quote: ... The 50 ml culture supernatant containing Strep-tagged CDA proteins was concentrated to 1 ml and exchanged to buffer W (100 mM Tris/HCl, 150 mM NaCl, 1 mM EDTA, pH 8) using 10 kD cutoff centrifugal filters (Sartorius, Germany) before being incubated with 25 µl of Strep-Tactin®XT agarose beads (IBA Lifesciences ...
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Maize AFP1 confers antifungal activity by inhibiting chitin deacetylases from a broad range of fungibioRxiv - Microbiology 2021Quote: ... A total 75 ml culture supernatant was concentrated and exchanged with buffer W using 10 kD cutoff centrifugal filters (Sartorius, Germany). A 50 µl of Strep-Tactin®XT agarose beads were added to the concentrated sample in a final volume of 1 ml containing 1x protease inhibitor cocktail and incubated overnight at 4 ℃ ...
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bioRxiv - Cell Biology 2022Quote: ... GCP6-tagged purified γ-TuRC was then subjected to buffer exchange using Vivaspin 500 centrifugal concentrator (10 kDa MWCO, Sartorius VS0102) for a final NaCl concentration of 150 mM in eluate ...
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bioRxiv - Molecular Biology 2022Quote: ... Proteolytic digestion was carried out according to the FASP protocol32 with 10 kDa molecular weight cutoff filters (Sartorius Vivacon 500 #VN01H02) using 30 ug of protein resulting from each fraction ...
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bioRxiv - Bioengineering 2023Quote: ... pH 7.0 to remove the salt and undesired low molecular weight (LMW) molecules (<10 kDa) from sample using 0.10 m2 Sartocon slice PES cassette (Sartorius Stedim, Germany) and AKTA flux 6 system (GE Healthcare ...
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bioRxiv - Molecular Biology 2023Quote: ... The media was then filtered through a 0.45 µm filter (STARLAB, Hamburg, Germany) and concentrated using a 10 KDa Vivaflow tangential flow filtration (TFF) membrane (Sartorius, Göttingen, Germany) to a volume of 10-15 ml ...
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bioRxiv - Bioengineering 2023Quote: Sylgard-184™ silicone elastomer kit PDMS pre-polymer was well mixed (5 mins) with curing agent at a 10:1 w/w ratio using a digital balance (Sartorius BP610) prior to vacuum desiccation and casting at various temperatures (60-90°C).
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bioRxiv - Molecular Biology 2023Quote: ... The cells were resuspended in 10 mL Tris buffer (50mM, pH 7.6) and afterwards lysed by using a dismembrator (Sartorius, Göttingen, Germany) at 1,600 rpm for 3 min ...
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bioRxiv - Cell Biology 2023Quote: ... the buffer was exchanged to 10 mM Tris pH 7.4 using Vivaspin 500 columns with 30000 Dalton cut off (Sartorius Stedim biotech). The samples were centrifuged (12000 g ...
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bioRxiv - Cancer Biology 2023Quote: ... The plates were spun down at 350g for 10 min at 4°C and placed in the Incucyte® Live-Cell Analysis System (Sartorius). The growth of the spheroids was monitored for the following 8 days and invasion was automatically quantified via the Incucyte® Spheroid module analysis tool ...
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bioRxiv - Microbiology 2023Quote: ... A sample of 6 liters of air per min was collected during the <10 min exposure on the 47 mm gelatin filter (Sartorius, Germany). Post exposure ...
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bioRxiv - Microbiology 2023Quote: ... were dialyzed against 20 mM Tris-HCl (pH 8.0) and concentrated to 10-15 mg/ml using Vivaspin Ultra concentrators (Sartorius, Göttingen, Germany). Purity of the proteins was analyzed after sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE ...
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bioRxiv - Cancer Biology 2023Quote: ... 25 µl of single-cell suspension was dispensed on ready-to-use plates with an 8-channel electronic Picus® pipette (10 to 300 µl, #735361, Sartorius).
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bioRxiv - Plant Biology 2023Quote: ... and centrifugation (10 min, 6,000 x g) the supernatant was membrane filtered (Minisart RC 15, 0.45 µm, Sartorius AG, Göttingen, Germany) and used for analysis ...
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bioRxiv - Microbiology 2024Quote: ... G3PDH containing fractions as judged by SDS-PAGE and activity measurements were pooled and imidazole was removed using a centrifugal concentrator (10 kDa cutoff, Sartorius, Germany). Protein aliquots were frozen in liquid nitrogen and stored at −80 °C for further use ...
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bioRxiv - Cell Biology 2020Quote: ... the supernatants from control and treated PACs (10 ml each) were concentrated separately to 2 ml with a Vivaspin 20 Ultrafiltration Unit (Sartorius Göttingen, Germany). The resulting samples were then subjected to protein precipitation to reduce the volume and enrich the proteins ...
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bioRxiv - Biochemistry 2020Quote: All decellularization fractions were digested using the filter aided sample preparation (FASP) protocol as previously described32 using 10 kDa molecular weight cutoff filters (Sartorius, Vivacon #VN01H02). Digests were performed for 16 hours in 25 mM ABC pH 8.0 using trypsin at a 1:100 enzyme:protein ratio at 37°C in an oven ...
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bioRxiv - Biochemistry 2020Quote: ... The peak fractions containing ANT(9) were pooled and concentrated to 6 mg/ml using a Vivaspin 10 kDa cut-off concentrator (Sartorius AG, Germany).
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bioRxiv - Microbiology 2021Quote: ... the buffer was exchanged to 50 mM Tris-HCl (pH 7.9) without sucrose using 10 kDa mass cutoff centrifugal filters (Sartorius Vivaspin 500, Sartorius AG, Göttingen, Germany), the samples were then brought to comparable protein concentrations and submitted to the Proteomics Facility of the University of Konstanz for protein mass fingerprinting ...
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bioRxiv - Immunology 2022Quote: ... The formulations were then diafiltrated against 100 x volume of Phosphate Buffered Saline (PBS) through a tangential-flow filtration (TFF) membrane with 10 kD molecular weight cut-offs (Sartorius Stedim Biotech), concentrated to a required concentration ...
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bioRxiv - Microbiology 2020Quote: ... filtered at 0.22 μm and concentrated to 1 mL using a Vivaspin turbo ultrafiltration units (10 kDa MWCO, Sartorius AG, Goettingen, Germany). Samples were further concentrated to 50 μL after five successive washes using Tris 50 mM ...
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bioRxiv - Cell Biology 2020Quote: ... the supernatants from control and treated PACs (10 ml each) were concentrated separately to 2 ml with a Vivaspin 20 Ultrafiltration Unit (Sartorius Göttingen, Germany). The resulting samples were then subjected to protein precipitation to reduce the volume and enrich the proteins ...
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bioRxiv - Biophysics 2022Quote: ... The peak fractions were collected and concentrated to <500 µL using a VivaSpin concentrator with 10 kDa cut-off (Sartorius Stedim Biotech). The concentrated protein was filtered through a 0.2 µm filter and injected on a Superdex 200 Increase 10/300 GL gel filtration column (GE Healthcare ...
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bioRxiv - Molecular Biology 2023Quote: ... The peak fractions were concentrated to 5-10 mg/ml with a 30-KDa cutoff Vivaspin Turbo 15 (Sartorius AG, Göttingen, Germany), flash-frozen in liquid nitrogen ...
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Cardiac fibroblasts regulate cardiomyocyte hypertrophy through dynamic regulation of type I collagenbioRxiv - Cell Biology 2022Quote: ... Thirty micrograms of protein resulting from each fraction was subjected to proteolytic digestion using a filter-aided sample preparation (FASP) protocol 20 with 10 kDa molecular weight cutoff filters (Sartorius Vivacon 500 #VN01H02). Samples were reduced with 5 mM tris(2-carboxyethylphosphine) ...
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bioRxiv - Microbiology 2021Quote: ... dispersed in deionized water with different pH were ultrasonically treated for 10 min at a frequency of 24 kHz (Sartorius labsonic M, Germany). The difference in the hydrodynamic diameter of the nanocomposites was investigated using a Zetasizer Nano-ZS-90 Dynamic Light Scattering (DLS ...
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bioRxiv - Immunology 2022Quote: ... or monitored in short time periods using a 10 x magnification in an Incucyte®S3 Live-Cell Analysis System (Sartorius, Göttingen, Germany). Image analysis was conducted with the machine learning software ilastik [80] using pixel and object classification to distinguish background from cell nuclei and to determine IRF3-GFP and H2B-mCherry colocalization ...
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bioRxiv - Biochemistry 2022Quote: ... The dialyzed 2C protein was centrifuged at 75,000 x g at 4 °C for 30 min to isolate soluble protein and concentrated using a Vivaspin Turbo 15 (Sartorius, 10 kDa MWCO) to 2 – 5 mL for preparative gel filtration ...
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bioRxiv - Molecular Biology 2023Quote: ... Proteolytic digestion of sample extracts was carried out according to the FASP protocol33 using 10 kDa molecular weight cutoff filters (Sartorius Vivacon 500 #VN01H02) and loading 10 ug of protein resulting from each fraction ...
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bioRxiv - Biochemistry 2023Quote: Samples were subjected to proteolytic digestion using a filter-aided sample preparation (FASP) protocol (Wisniewski et al. 2009) with 10 kDa molecular weight cutoff filters (Sartorius Vivacon 500 #VN01H02). Samples were reduced with 5 mM tris(2-carboxyethyl)phosphine) ...
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bioRxiv - Biophysics 2020Quote: ... The flow-through of the column was concentrated (Vivaspin®, mwco 10 or 30 kDa for single SBD or tandem mutants, Sartorius; ~ 5 mg/ml), dialyzed in buffer A supplemented with 50 % glycerol ...
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bioRxiv - Evolutionary Biology 2019Quote: ... The fractions containing MGE protein were pooled and concentrated to 1 mL by using a centrifugal concentrator (Sartorius, 10 kDa MWCO, Vivaspin turbo 15). The recombinant proteins were further purified by ion exchange and gel filtration chromatography with ÄKTAprime plus system ...
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bioRxiv - Evolutionary Biology 2019Quote: ... The fractions containing MGE protein were pooled and concentrated to 1 mL by using a centrifugal concentrator (Sartorius, 10 kDa MWCO, Vivaspin turbo 15). For gel filtration ...
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bioRxiv - Molecular Biology 2022Quote: ... were mixed together as well as eluates from samples (Pho92-HTP x 2 reps + Pho92-HTP ime4Δ/Δ x 2 reps) before concentration with Vivacon® 500 filtration cartridges (10 kDa MWCO; Sartorius, Cat# VN01H02) to a final volume of 120 μL ...
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bioRxiv - Bioengineering 2023Quote: ... Batch reactors were incubated for 10 days at a mesophilic temperature of 35°C in a Certomat® BS1 incubator (Sartorius Stedim Biotech, Germany) under continuous agitation (145 rpm) ...