Labshake search
Citations for Sartorius :
51 - 100 of 207 citations for Hyaluronidase PH 20 SPAM1 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2021Quote: ... to 0.1 M Glycine / 10 mM Tris / pH 7.5 using a Sartocon Slice ECO (30 kDa MWCO, 0.14 m²) PES membrane (Sartorius). Trimer-containing fractions were pooled and passed over a pre-equilibrated MAbSelect SuRe Protein A (ProA ...
-
bioRxiv - Immunology 2022Quote: ... The Octet Acquisition v10.0 program was used to set the following steps for KD measurements: Pre-conditioning (0.2 M glycine, pH 1.52 & 10x kinetic buffer (KB) (Cat 18-1105, Sartorius), 30 s) ...
-
bioRxiv - Microbiology 2023Quote: ... Purified protein fractions were dialyzed against 1X PBS (pH 7.4) using the Vivaspin 10 kDa centrifuge system (Sartorius). Protein quantification was performed in a spectrophotometer at 280 nm and the assays were performed with the enzyme at a concentration of 5.88 μM.
-
bioRxiv - Molecular Biology 2023Quote: ... pH 6.0) using a centrifugal concentrator with a molecular weight cutoff of 10 kDa (Vivaspin 6, VS0602, Sartorius). The presence of the fucosidase (62 kDa ...
-
bioRxiv - Microbiology 2023Quote: ... then concentrated using a 3kDa Vivaspin 20 centrifugal concentrator column (Sartorius) to give a final volume of approximately 1000 μL ...
-
bioRxiv - Bioengineering 2023Quote: ... Fractions containing encapsulin were combined and concentrated by Vivaspin 20 (Sartorius) spin filters (100 KDa cutoff ...
-
bioRxiv - Cell Biology 2023Quote: ... Fragmented DNA was concentrated using Vivaspin 20 columns (Sartorius; Cat #VS2032), and unspecific binding was blocked by incubating the sample with 1% BSA ...
-
bioRxiv - Neuroscience 2024Quote: ... Samples were concentrated using Vivaspan 20 with a 3000 MWCO (Sartorius Stedim Biotech GmbH ...
-
bioRxiv - Biophysics 2019Quote: ... purified CysRsaA was buffer exchanged into 50 mM HEPES pH 7.0 and 150 mM NaCl using a 30 kDa MWCO centrifugal concentrator (Sartorius). Overnight labeling of CysRsaA protein (>20 µM ...
-
bioRxiv - Biochemistry 2020Quote: ... 150 mM NaCl pH 7.5 by ultrafiltration using 100 kDa molecular weight cut-off (MWCO) spin concentrator tubes (Vivaspin, Sartorius). Protein concentration was then determined using absorbance at 280 nm (ε280 = 14,565 mol−1cm−1 ...
-
bioRxiv - Biophysics 2020Quote: ... 250 mM) at pH 7.5 and 4°C via centrifugal filter units (13000 x g, Vivaspin 500, MWCO 10000 (Sartorius) or Micro Bio-Spin 6 columns (Bio-Rad) ...
-
bioRxiv - Microbiology 2021Quote: ... the buffer was exchanged to 50 mM Tris-HCl (pH 7.9) without sucrose using 10 kDa mass cutoff centrifugal filters (Sartorius Vivaspin 500 ...
-
bioRxiv - Biochemistry 2022Quote: ... and buffer was exchanged four times with 5 mL of 10 mM potassium phosphate buffer pH 7.4 using a Vivaspin 6 Centrifugal Concentrator (Sartorius) at 2,000 rcf and 4°C for 20 min ...
-
bioRxiv - Cancer Biology 2022Quote: ... The proteins were resuspended in 200 μL 8 M urea (pH 8.0) and loaded in 30 kD Microcon filter tubes (Sartorius) and centrifuged at 12,000 g for 20 min ...
-
bioRxiv - Synthetic Biology 2022Quote: Purified NT2RepCT (suspended in 20 mM Tris-HCl pH 8.0 buffer) was concentrated to approximately 100 mg/mL using a Vivaspin centrifugal concentrator (Sartorius) with 10 kDa molecular-weight cutoff.
-
bioRxiv - Biochemistry 2022Quote: ... and then the supernatant was concentrated and exchanged into Buffer B (25 mM HEPES pH 7.5 and 150 mM NaCl) using a Hydrosart Ultrafilter system (Sartorius). The following purification steps for anti-BRIL Fab were the same as that of BRIL-SLC19A1 ...
-
bioRxiv - Cell Biology 2023Quote: ... samples were denatured in UA buffer (8 M urea in 0.1 M Tris/HCl pH 8.5) and centrifuged on a 30 kDa cut-off filter device (PALL or Sartorius) and washed thrice with UA buffer and twice with 50 mM ammoniumbicarbonate (ABC) ...
-
bioRxiv - Microbiology 2023Quote: ... Cytiva) in 50 mM sodium acetate (pH 5.2) and concentrated using Vivaspin polyethersulfone membrane (3 kDa cut-off, Sartorius). Protein concentrations were determined using the Bradford method (Bio-Rad ...
-
bioRxiv - Bioengineering 2024Quote: ... and arginine (pH 6) to a final concentration of ≥200 mg/mL using the Ambr Crossflow system (Sartorius, Germany).
-
bioRxiv - Biochemistry 2020Quote: ... 5% ethylene glycol), followed by buffer exchange to (30 mM Tris/HCl, pH 8.0) with Vivaspin centrifugal concentrator (Sartorius, Germany). The final phosphoprotein concentrations were adjusted to 1.5 mg/ml (80 μM) ...
-
bioRxiv - Biochemistry 2020Quote: ... were exchanged into phosphate buffer (pH 7.4) using disposable Vivaspin® 500 centrifugal concentrators with an MWCO of 3 kDa (Sartorius) to remove Cu2+ or H202 ...
-
bioRxiv - Biophysics 2022Quote: FITC: AcmJRL was diluted in Na2CO3-buffer (100 mM, pH 9.3) and concentrated (Vivaspin, Sartorius Stedim Biotech GmbH, 10,000 MWCO) to yield a final protein concentration of 78 μM (1.8 mg in 1.5 mL) ...
-
bioRxiv - Microbiology 2021Quote: ... and virus particles were enriched in Vivaspin 20 columns (Sartorius Stedim, Biotech) via centrifugation ...
-
bioRxiv - Microbiology 2021Quote: ... The protein was concentrated with Vivaspin 20 concentrators (30 kDa MWCO, Sartorius). Concentrated protein was further purified by size exclusion chromatography on a HiLoad 16/600 Superdex 200 pg column (Cytiva) ...
-
bioRxiv - Physiology 2022Quote: ... 2 mM DTT) using a Vivaspin-20 concentrator (catalog no. VS2021, Sartorius). Five buffer exchanges with the storage buffer were performed and purified MBP was then aliquoted and stored at −80°C.
-
bioRxiv - Biochemistry 2020Quote: ... pooled and concentrated using a Vivaspin® 20 5000 Mw concentrator (Sartorius). The concentrated protein was dialyzed overnight at 4°C to remove the imidazole ...
-
bioRxiv - Microbiology 2021Quote: ... the virus supernatant was concentrated using Vivaspin 20 concentrators (Sartorius Stedim Biotech) by spinning at 6000 g for 30 min ...
-
bioRxiv - Biochemistry 2023Quote: ... Fractions containing BLMcore were concentrated in Vivaspin-20 10 kDa concentrator (Sartorius) to 2 mL and loaded onto HiPrep 16/60 Sephacryl S-300 HR column (Cytiva ...
-
bioRxiv - Molecular Biology 2023Quote: Conditioned medium was concentrated using centrifugal concentrators (Vivaspin 20/Vivacell 70, Sartorius) or pressure concentrators (Vivacell 250 ...
-
bioRxiv - Biochemistry 2024Quote: ... before centrifugal concentration with a 30 kDa cut-off (Vivaspin 20, Sartorius). Crystals for preparing seeds were produced by combining (1:1 ...
-
Maize AFP1 confers antifungal activity by inhibiting chitin deacetylases from a broad range of fungibioRxiv - Microbiology 2021Quote: ... The 50 ml culture supernatant containing Strep-tagged CDA proteins was concentrated to 1 ml and exchanged to buffer W (100 mM Tris/HCl, 150 mM NaCl, 1 mM EDTA, pH 8) using 10 kD cutoff centrifugal filters (Sartorius, Germany) before being incubated with 25 µl of Strep-Tactin®XT agarose beads (IBA Lifesciences ...
-
bioRxiv - Molecular Biology 2023Quote: ... The cells were resuspended in 2 mL 50 mM Tris buffer (pH 7.6) and lysed by using a dismembrator (Sartorius, Göttingen, Germany) at 1,600 rpm for 3 min ...
-
bioRxiv - Cell Biology 2023Quote: ... the buffer was exchanged to 10 mM Tris pH 7.4 using Vivaspin 500 columns with 30000 Dalton cut off (Sartorius Stedim biotech). The samples were centrifuged (12000 g ...
-
bioRxiv - Cell Biology 2023Quote: ... The formulations were then diafiltrated against a 10 × volume of PBS (pH 7.4) through a tangential-flow filtration membrane with a 100 kDa molecular weight cut-off (Sartorius Stedim Biotech), concentrated to the desired concentrations ...
-
bioRxiv - Molecular Biology 2023Quote: ... The cells were resuspended in 10 mL Tris buffer (50mM, pH 7.6) and afterwards lysed by using a dismembrator (Sartorius, Göttingen, Germany) at 1,600 rpm for 3 min ...
-
bioRxiv - Developmental Biology 2021Quote: ... Cell media containing lentiviral particles were concentrated using centricon column (Vivaspin 20, Sartorius). Each viral production was titrated beforehand to establish a multiplicity of infection (MOI ...
-
bioRxiv - Microbiology 2021Quote: ... The flow-through was concentrated using Vivaspin 20 concentrators (30 kDa MWCO, Sartorius). Concentrated protein was further purified by size exclusion chromatography on a HiLoad 26/600 Superdex 200 pg column (Cytiva) ...
-
bioRxiv - Biophysics 2021Quote: ... 50 mM KCl) using Vivaspin 20 columns (10 kDa molecular weight cutoff, Sartorius). This step was followed by cation exchange chromatography using a Mono-S-HR 10/10 FPLC column pre-equilibrated with SAU-50 and SAU-1000 buffers ...
-
bioRxiv - Microbiology 2022Quote: ... We concentrated the elution by centrifugation in a VivaSpin® 20 column (Sartorius). We performed refolding by dialysis in a 3,500-5,000 molecular weight cutoff Float-a-lyzer® G2 device (Spectrum Labs) ...
-
bioRxiv - Microbiology 2020Quote: ... concentrated and purified using Vivaspin 20 ultrafiltration units (300kDa MW cut-off, Sartorius), and stored at −80°C.
-
bioRxiv - Biophysics 2021Quote: ... Fractions containing ParA2vc were concentrated using a VivaSpin 20 column 10,000 MWCO (Sartorius), before snap freezing in Liquid nitrogen for storage at −80°C ...
-
bioRxiv - Molecular Biology 2021Quote: ... was concentrated to 0.5 mL on a Vivaspin 20 MWCO 30 filter (Sartorius) and was injected into a Superose 6 Increase 10/300 column (GE Healthcare ...
-
bioRxiv - Immunology 2020Quote: ... Purified viral particles were obtained by Ion Exchange (VivaPure® AdenoPACKTM 20, Sartorius), and the DNA extracted and subjected to Next Generation Sequencing ...
-
bioRxiv - Biochemistry 2023Quote: ... All monomeric Grb2 fractions were pooled and concentrated with Vivaspin 20 (Sartorius AG). The purification was analyzed by SDS-PAGE and the protein concentration was determined by UV/Vis spectroscopy at 280 nm using a Jasco J-650 spectrometer.
-
bioRxiv - Biophysics 2024Quote: ... all samples were concentrated to 300 μM TTR in Vivaspin 20 concentrators (Sartorius) and added to 5 mm susceptibility-matched Shigemi tubes with 5% D2O.
-
bioRxiv - Microbiology 2021Quote: ... the buffer was exchanged to 50 mM Tris-HCl (pH 7.9) without sucrose using 10 kDa mass cutoff centrifugal filters (Sartorius Vivaspin 500, Sartorius AG, Göttingen, Germany), the samples were then brought to comparable protein concentrations and submitted to the Proteomics Facility of the University of Konstanz for protein mass fingerprinting ...
-
bioRxiv - Molecular Biology 2020Quote: ... The active fractions as identified by a phenol-hypochlorite assay (Weatherburn, 1967) were buffer-exchanged to 50mM Tris pH 7.0 within a centrifugal filter unit (Sartorius, Vivaspin MWCO 50kDa) and applied to a Mono Q HR 5/5 column pre-equilibrated with 50mM TrisHCl ...
-
bioRxiv - Microbiology 2022Quote: The purified proteins were concentrated and exchanged to 10 mM sodium citrate buffer (pH 5.0) using Vivaspin® Turbo 4 ultrafiltration units with a 5000 MWCO PES membrane (Sartorius; Cat.# VS04T11). The purity and concentration of each PcaLOOL was assessed by SDS-PAGE and the Pierce™ BCA Protein Assay Kit ...
-
bioRxiv - Microbiology 2020Quote: ... The eluate was then concentrated using Vivaspin® 20 10,000 MWCO concentrator columns (Sartorius) and desalted into 25mM Tris pH 8 ...
-
bioRxiv - Microbiology 2021Quote: ... Peak fractions were pooled and concentrated with Vivaspin 20 concentrators (10 kDa MWCO, Sartorius). Concentrated protein was further purified by size exclusion chromatography on a HiLoad 16/600 Superdex 75 pg column (Cytiva) ...