Labshake search
Citations for Sartorius :
201 - 250 of 283 citations for Human Putative Alpha 1 Antitrypsin Related Protein SERPINA2 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2022Quote: ... concentrated in 1 mL in a Vivaspin Turbo 4 (30’000 MWCO, Sartorius VS04T22) and further purified on a Superdex 75 10/300 gel filtration column (GE Healthcare).
-
bioRxiv - Immunology 2020Quote: ... concentrated to 1-5 mg/ml by ultrafiltration using appropriate VivaSpin devices (Sartorius), were snap-frozen in liquid nitrogen in small aliquots and stored at -80°C.
-
bioRxiv - Biochemistry 2021Quote: ... 1 mM DTT) and concentrated (∼100 µM) using centrifugal filters (Vivaspin 500, Sartorius). In the apo condition ...
-
bioRxiv - Immunology 2023Quote: ... resuspended in phenol red-free RPMI containing Cytotox Green (1/4000 diluted, Sartorius). Tumor cells were washed twice with phenol red-free RPMI ...
-
bioRxiv - Microbiology 2020Quote: ... 1 mM EDTA) for 1.5 min at 2000 rpm in the Mikro-Dismembrator (Sartorius). Resulting crude extracts were subjected to sonication in 30 ml buffer and afterwards centrifuged twice at 13.000 rpm and 4 °C ...
-
bioRxiv - Biochemistry 2021Quote: ... to a minimum response value of 1 nm on the Octet Red96 System (Sartorius). A baseline response was established in assay buffer prior to each association ...
-
bioRxiv - Neuroscience 2022Quote: ... to a minimum response value of 1 nm on the Octet Red96 System (Sartorius). For measurements of amyloid beta ...
-
bioRxiv - Neuroscience 2022Quote: ... to a minimum response value of 1 nm on the Octet Red96 System (Sartorius). Purified mTfR or VHH clones were diluted into assay buffer at the specified concentrations ...
-
bioRxiv - Microbiology 2023Quote: ... filtered through a slow filter paper (#293; 1–2 µm Sartorius, Thermo Fisher Scientific), and then used to determine electrical conductivity (EC) ...
-
bioRxiv - Molecular Biology 2024Quote: ... and ZIP cells (1:1000 dilution, #4642 and #4641, Sartorius, Ann Arbor, MI - USA) immediately before time 0 of IncuCyte scan ...
-
bioRxiv - Bioengineering 2021Quote: ... Each system (Figure 1) consisted of a bioreactor Biostat A plus (Sartorius AG, Göttingen, Germany) with 1.0 L working volume ...
-
bioRxiv - Neuroscience 2020Quote: ... the flasks were placed into a temperature-controlled shaker Certomat® BS-1 (Sartorius Group) for 17-20 hours and shaken at 180 rpm at 37 °C to detach non-astroglial cells from the flask ...
-
bioRxiv - Biochemistry 2021Quote: ... concentrated to 1 mL using a Vivaspin 4 Turbo concentrator (Cut-off 5 kDa; Sartorius), and purified via size-exclusion chromatography with a Superdex S75-10/300 column (GE Healthcare).
-
bioRxiv - Synthetic Biology 2020Quote: The bioreactor cultures were performed in 1 L bioreactors (Biostat Q Plus, Sartorius, Gottingen, Germany) and started with a batch phase using 400 mL synthetic medium containing 20 g/L of glucose and 5 mL Antifoam 204 (Sigma A6426) ...
-
bioRxiv - Synthetic Biology 2022Quote: The batch fermentations were performed in BIOSTAT™ Qplus 1-L bioreactors (Sartorius, Göttingen, Germany) with a working culture volume of 800 mL ...
-
bioRxiv - Plant Biology 2023Quote: ... and plants were weighed with a BSA224S-CW 1/10,000 analytical balance (Sartorius, Beijing, China). Three biological replicate samples were analyzed for these assays (Chen et al. ...
-
bioRxiv - Neuroscience 2021Quote: ... 1 mM EDTA containing Complete® EDTA-free protease inhibitors using a Potter S homogenizer (Sartorius). After addition of 1% (v/v ...
-
bioRxiv - Cell Biology 2021Quote: ... fresh medium was supplemented with 1:10 000 IncuCyte® Cytotox Green Reagent (Sartorius, Göttingen, Germany) to determine dead cells ...
-
bioRxiv - Biochemistry 2023Quote: ... concentrated to 5 mg mL-1 using a 100 kDa molecular weight cut off concentrator (Sartorius), and processed immediately for cryo-electron microscopy.
-
bioRxiv - Molecular Biology 2020Quote: ... Fractions containing A2M tetramers were combined and concentrated to 1 ml final volume (Vivaspin Turbo 15, Sartorius).
-
bioRxiv - Cancer Biology 2021Quote: ... HA-Ub75-thioester was concentrated to a concentration of ∼1 mg/ml using 3,000 MW filters (Sartorius) and then desalted against PBS using a PD10 column (GE Healthcare) ...
-
bioRxiv - Immunology 2020Quote: ... Fermentation was conducted using a Biostat Qplus Bioreactor with a 1 L vessel (Sartorius Stedim, Guxhagen, Germany). For 5 L runs ...
-
bioRxiv - Microbiology 2022Quote: A microbial community was enriched in a 1-l bioreactor (BIOSTAT® A plus, Sartorius, Göttingen, Germany), inoculated with broth from a former study [16] and fed with mineral medium containing xylan and lactate over 150 days [13] ...
-
bioRxiv - Plant Biology 2023Quote: ... The purest fractions were combined and concentrated to 1 ml using Vivaspin 30K centrifugal concentrators (Sartorius, VS0621). The protein sample was then further purified by size exclusion chromatography on a HiLoad 16/600 Superdex 200 pg column (Cytiva ...
-
bioRxiv - Biochemistry 2023Quote: ... The fractions containing the complex were concentrated to 1 mg/mL using a Vivaspin centrifugal concentrator (Sartorius).
-
bioRxiv - Microbiology 2024Quote: ... A global association 1:2 bivalent analyte model using the Octet Data Analysis HT software v7 (Sartorius) was used to account for the dimeric HAstV-VA1 spike ...
-
bioRxiv - Synthetic Biology 2022Quote: ... The seed culture from each flask was transferred to a 1-L bioreactor (Biostat B-DCU, Sartorius, Germany) with 0.7 L fermentation medium ...
-
bioRxiv - Neuroscience 2022Quote: ... was diluted into assay buffer at 1 µg/mL and immobilized onto streptavidin coated biosensors (#18-5019, Sartorius) to a minimum response value of 1 nm on the Octet Red96 System (Sartorius) ...
-
bioRxiv - Microbiology 2023Quote: ... and the wells were rinsed twice with 1× phosphate-buffered saline (PBS, obtained from Sartorius, Beit HaEmek, Israel). This process aimed to remove non-adherent bacterial cells ...
-
bioRxiv - Cell Biology 2023Quote: Fractions from the gel filtration peak were concentrated to 1 mg/mL using a Vivaspin® Turbo (Sartorius) centrifugal filter ...
-
bioRxiv - Molecular Biology 2023Quote: ... was added at 1:1000 dilution and live cell imaging was performed on the Incucyte S3 System (Sartorius). Apoptosis was calculated using the S3 Live Cell Analysis Instrument (Sartorius ...
-
bioRxiv - Microbiology 2020Quote: ... 1 mM TCEP,10% glycerol) and concentrated using Vivaspin turbo with a 10 kDa molecular mass cutoff concentrator (Sartorius) to a final concentration of 1 mg/mL ...
-
bioRxiv - Molecular Biology 2020Quote: ... The sera from two healthy donors (1.5 ml) were diluted two-fold and concentrated (to 1 ml final volume) using Vivaspin Turbo 15 (Sartorius) units ...
-
bioRxiv - Microbiology 2020Quote: ... Samples (1 mL) from the liquid phase of the cultures were filtered (MiniSart RC4, 0.2 μm, Sartorius, Göttingen, Germany) and acidified with 2.5 μL H2SO4 ...
-
bioRxiv - Biochemistry 2022Quote: ... samples were further concentrated to ~1-2 mg/mL with a 100 kDa MWCO Vivaspin 500 centrifugal concentrator (Sartorius) at 12000 ×g ...
-
bioRxiv - Microbiology 2022Quote: ... 5 mM MgCl2) and concentrated to ~1 mg/mL by Vivaspin 20 centrifugal filter unit (MWCO 10 kDa) (Sartorius). Sfp synthase and CoA-conjugated dyes were prepared as previously described54 and used to perform the overnight Fab labeling reaction on ice ...
-
bioRxiv - Systems Biology 2022Quote: ... The culture pH was kept at 5.0 by automatic addition of 0.5 mol.l-1 KOH (BioStat controller, type 8843415, Sartorius AG, Germany).
-
bioRxiv - Cell Biology 2023Quote: ... samples were denatured in UA buffer (8 M urea in 0.1 M Tris/HCl pH 8.5) and centrifuged on a 30 kDa cut-off filter device (PALL or Sartorius) and washed thrice with UA buffer and twice with 50 mM ammoniumbicarbonate (ABC) ...
-
bioRxiv - Cancer Biology 2023Quote: ... PBMCs were labelled with 1:1000 Incucyte® Nuclight Rapid Red Dye for Live-Cell Nuclear Labeling Incucyte (Sartorius) while cells death was measured by adding the IncuCyte Caspase 3/7 Green Apoptosis Assay Reagent 1:5000 to the cocultures (Essen BioScience) ...
-
bioRxiv - Microbiology 2023Quote: ... Vesicles were purified from 1 L of culture per replicate using a tangential flow filtration system (Vivaflow 200, Sartorius). Details on all methods ...
-
bioRxiv - Pharmacology and Toxicology 2024Quote: ... in the presence of a green mortality probe (1/3000, Incucyte® Caspase-3/7 Green Dye - Sartorius, Germany). Green fluorescence was immediately followed every 90 or 120 minutes with an Incucyte® S3 Live-Cell Analysis system (Sartorius ...
-
bioRxiv - Molecular Biology 2024Quote: ... PBMCs were labelled with 1:1000 Incucyte® Nuclight Rapid Red Dye for Live-Cell Nuclear Labeling Incucyte (Sartorius) while cell death was measured by the IncuCyte Caspase 3/7 Green Apoptosis Assay Reagent as explained before ...
-
bioRxiv - Microbiology 2019Quote: ... The eluted complex was then concentrated to 1 mL using a Vivaspin 4 Turbo concentrator (Cut-off 5 kDa; Sartorius). A final purification step was performed using size-exclusion chromatography by loading the proteins on a HiLoad 10/300 Superdex 200 column (GE Healthcare ...
-
bioRxiv - Biophysics 2019Quote: ... three successive 1:30 dilutions were performed to remove unbound dye molecules using a 30 kDa MWCO centrifugal concentrator (Sartorius) and buffer containing 50 mM Tris/HCl pH 8.0 and 150 mM NaCl ...
-
bioRxiv - Biochemistry 2020Quote: ... the purified NT-mini/NT*-mini was concentrated to 1 mg/mL with a Vivaspin Turbo10 kDa MWCO concentrator (Sartorius), flash frozen in liquid nitrogen and stored at −80 °C ...
-
bioRxiv - Biophysics 2020Quote: ... Peak fractions containing purified Cx46/50 were pooled and concentrated to 5–6 mg mL-1 with a centrifugal device (Vivaspin 6; 50-kDa cut-off filter; Sartorius). Protein concentration was determined by UV absorbance at 280 nm ...
-
bioRxiv - Immunology 2021Quote: ... IncuCyte® Annexin V Green Reagent was added directly in the medium at the dilution 1:200 and B cells were imaged every hour for 3.5 days using IncuCyte® Live-Cell Analysis System (Sartorius).
-
bioRxiv - Neuroscience 2022Quote: ... biotinylated VHH was diluted into assay buffer at 1 µg/mL and immobilized onto streptavidin coated biosensors (#18-5019, Sartorius) to a minimum response value of 1 nm on the Octet Red96 System (Sartorius) ...
-
bioRxiv - Neuroscience 2022Quote: ... The pooled fractions of the NOTCH3 EGF1-5 WT and R133C were concentrated to 1 mg/mL in a concentration column (Vivaspin 6 10 KDa MWCO, Sartorius) according to the manufacturer’s instructions ...
-
bioRxiv - Immunology 2022Quote: ... His-tagged SARS-CoV-2 RBD was prepared at 8 μg ml−1 and loaded on prehydrated anti-penta-HIS biosensors (Sartorius) for 3 min ...