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Citations for Sartorius :
301 - 350 of 363 citations for Human Immunodeficiency Virus Tat Protein HIV 1 Clade C TV019 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... concentrated to 5 mg mL-1 using a 100 kDa molecular weight cut off concentrator (Sartorius), and processed immediately for cryo-electron microscopy.
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bioRxiv - Molecular Biology 2020Quote: ... Fractions containing A2M tetramers were combined and concentrated to 1 ml final volume (Vivaspin Turbo 15, Sartorius).
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bioRxiv - Cancer Biology 2021Quote: ... HA-Ub75-thioester was concentrated to a concentration of ∼1 mg/ml using 3,000 MW filters (Sartorius) and then desalted against PBS using a PD10 column (GE Healthcare) ...
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bioRxiv - Immunology 2020Quote: ... Fermentation was conducted using a Biostat Qplus Bioreactor with a 1 L vessel (Sartorius Stedim, Guxhagen, Germany). For 5 L runs ...
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bioRxiv - Microbiology 2022Quote: A microbial community was enriched in a 1-l bioreactor (BIOSTAT® A plus, Sartorius, Göttingen, Germany), inoculated with broth from a former study [16] and fed with mineral medium containing xylan and lactate over 150 days [13] ...
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bioRxiv - Plant Biology 2023Quote: ... The purest fractions were combined and concentrated to 1 ml using Vivaspin 30K centrifugal concentrators (Sartorius, VS0621). The protein sample was then further purified by size exclusion chromatography on a HiLoad 16/600 Superdex 200 pg column (Cytiva ...
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bioRxiv - Biochemistry 2023Quote: ... The fractions containing the complex were concentrated to 1 mg/mL using a Vivaspin centrifugal concentrator (Sartorius).
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bioRxiv - Microbiology 2024Quote: ... A global association 1:2 bivalent analyte model using the Octet Data Analysis HT software v7 (Sartorius) was used to account for the dimeric HAstV-VA1 spike ...
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bioRxiv - Synthetic Biology 2022Quote: ... The seed culture from each flask was transferred to a 1-L bioreactor (Biostat B-DCU, Sartorius, Germany) with 0.7 L fermentation medium ...
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bioRxiv - Neuroscience 2022Quote: ... was diluted into assay buffer at 1 µg/mL and immobilized onto streptavidin coated biosensors (#18-5019, Sartorius) to a minimum response value of 1 nm on the Octet Red96 System (Sartorius) ...
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bioRxiv - Microbiology 2023Quote: ... and the wells were rinsed twice with 1× phosphate-buffered saline (PBS, obtained from Sartorius, Beit HaEmek, Israel). This process aimed to remove non-adherent bacterial cells ...
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bioRxiv - Cell Biology 2023Quote: Fractions from the gel filtration peak were concentrated to 1 mg/mL using a Vivaspin® Turbo (Sartorius) centrifugal filter ...
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bioRxiv - Molecular Biology 2023Quote: ... was added at 1:1000 dilution and live cell imaging was performed on the Incucyte S3 System (Sartorius). Apoptosis was calculated using the S3 Live Cell Analysis Instrument (Sartorius ...
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bioRxiv - Microbiology 2020Quote: ... 1 mM TCEP,10% glycerol) and concentrated using Vivaspin turbo with a 10 kDa molecular mass cutoff concentrator (Sartorius) to a final concentration of 1 mg/mL ...
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bioRxiv - Molecular Biology 2020Quote: ... The sera from two healthy donors (1.5 ml) were diluted two-fold and concentrated (to 1 ml final volume) using Vivaspin Turbo 15 (Sartorius) units ...
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bioRxiv - Microbiology 2020Quote: ... Samples (1 mL) from the liquid phase of the cultures were filtered (MiniSart RC4, 0.2 μm, Sartorius, Göttingen, Germany) and acidified with 2.5 μL H2SO4 ...
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bioRxiv - Biochemistry 2022Quote: ... samples were further concentrated to ~1-2 mg/mL with a 100 kDa MWCO Vivaspin 500 centrifugal concentrator (Sartorius) at 12000 ×g ...
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bioRxiv - Microbiology 2022Quote: ... 5 mM MgCl2) and concentrated to ~1 mg/mL by Vivaspin 20 centrifugal filter unit (MWCO 10 kDa) (Sartorius). Sfp synthase and CoA-conjugated dyes were prepared as previously described54 and used to perform the overnight Fab labeling reaction on ice ...
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bioRxiv - Systems Biology 2022Quote: ... The culture pH was kept at 5.0 by automatic addition of 0.5 mol.l-1 KOH (BioStat controller, type 8843415, Sartorius AG, Germany).
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bioRxiv - Cell Biology 2023Quote: ... samples were denatured in UA buffer (8 M urea in 0.1 M Tris/HCl pH 8.5) and centrifuged on a 30 kDa cut-off filter device (PALL or Sartorius) and washed thrice with UA buffer and twice with 50 mM ammoniumbicarbonate (ABC) ...
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bioRxiv - Cancer Biology 2023Quote: ... PBMCs were labelled with 1:1000 Incucyte® Nuclight Rapid Red Dye for Live-Cell Nuclear Labeling Incucyte (Sartorius) while cells death was measured by adding the IncuCyte Caspase 3/7 Green Apoptosis Assay Reagent 1:5000 to the cocultures (Essen BioScience) ...
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bioRxiv - Microbiology 2023Quote: ... Vesicles were purified from 1 L of culture per replicate using a tangential flow filtration system (Vivaflow 200, Sartorius). Details on all methods ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... in the presence of a green mortality probe (1/3000, Incucyte® Caspase-3/7 Green Dye - Sartorius, Germany). Green fluorescence was immediately followed every 90 or 120 minutes with an Incucyte® S3 Live-Cell Analysis system (Sartorius ...
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bioRxiv - Molecular Biology 2024Quote: ... PBMCs were labelled with 1:1000 Incucyte® Nuclight Rapid Red Dye for Live-Cell Nuclear Labeling Incucyte (Sartorius) while cell death was measured by the IncuCyte Caspase 3/7 Green Apoptosis Assay Reagent as explained before ...
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bioRxiv - Microbiology 2019Quote: ... The eluted complex was then concentrated to 1 mL using a Vivaspin 4 Turbo concentrator (Cut-off 5 kDa; Sartorius). A final purification step was performed using size-exclusion chromatography by loading the proteins on a HiLoad 10/300 Superdex 200 column (GE Healthcare ...
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bioRxiv - Biophysics 2019Quote: ... three successive 1:30 dilutions were performed to remove unbound dye molecules using a 30 kDa MWCO centrifugal concentrator (Sartorius) and buffer containing 50 mM Tris/HCl pH 8.0 and 150 mM NaCl ...
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bioRxiv - Biochemistry 2020Quote: ... the purified NT-mini/NT*-mini was concentrated to 1 mg/mL with a Vivaspin Turbo10 kDa MWCO concentrator (Sartorius), flash frozen in liquid nitrogen and stored at −80 °C ...
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bioRxiv - Biophysics 2020Quote: ... Peak fractions containing purified Cx46/50 were pooled and concentrated to 5–6 mg mL-1 with a centrifugal device (Vivaspin 6; 50-kDa cut-off filter; Sartorius). Protein concentration was determined by UV absorbance at 280 nm ...
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bioRxiv - Immunology 2021Quote: ... IncuCyte® Annexin V Green Reagent was added directly in the medium at the dilution 1:200 and B cells were imaged every hour for 3.5 days using IncuCyte® Live-Cell Analysis System (Sartorius).
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bioRxiv - Neuroscience 2022Quote: ... biotinylated VHH was diluted into assay buffer at 1 µg/mL and immobilized onto streptavidin coated biosensors (#18-5019, Sartorius) to a minimum response value of 1 nm on the Octet Red96 System (Sartorius) ...
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bioRxiv - Neuroscience 2022Quote: ... The pooled fractions of the NOTCH3 EGF1-5 WT and R133C were concentrated to 1 mg/mL in a concentration column (Vivaspin 6 10 KDa MWCO, Sartorius) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... His-tagged SARS-CoV-2 RBD was prepared at 8 μg ml−1 and loaded on prehydrated anti-penta-HIS biosensors (Sartorius) for 3 min ...
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bioRxiv - Bioengineering 2022Quote: ... Eluate from column 1 above 20mAu was loaded into a 1mL prepacked HyperCel STAR AX column (Sartorius AG, Gottingen, Germany). Flow-through material above 20mAu was collected.
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bioRxiv - Immunology 2023Quote: ... two-fold serial dilutions of the biotinylated avi-tagged CH505 TF chSOSIPv4.1 and Man5GlcNAc2-enriched CH505 M5.G458Y chSOSIPv4.1 Env trimers were immobilized on hydrated Streptavidin (SA) Tips (Octet® Sartorius) for 120 seconds ...
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bioRxiv - Genetics 2023Quote: ... 500 cells were seeded in duplicates in 96-well plates and treated with 1 µg/ml dox for 9-10 days and analyzed with Incucyte (Sartorius). Untreated cells served as reference.
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bioRxiv - Synthetic Biology 2022Quote: ... passivated (with 20 μL of 50 mM ABC in 1% sodium deoxycholate or SDC) Vivacon 500 3 kDa molecular weight cutoff (MWCO) filtration units from Sartorius Stedim Biotech ...
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bioRxiv - Systems Biology 2023Quote: ... Cell dry weight was determined by vacuum filtration of 1 mL of samples on pre-weighed 0.45 µm filter membranes (Sartorius Biolab), followed by 15 min microwaving at 325 W and placement in a desiccator for at least 3 days.
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bioRxiv - Microbiology 2023Quote: ... monoclonal antibodies or VHHs were added to transfected cells at a final concentration of 1 µg/ml and GFP expression was monitored hourly over time with an Incucyte Zoom live cell analysis device (Sartorius) and analyzed with the provided Incucyte software.
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bioRxiv - Cell Biology 2023Quote: ... The remaining vesicle pellets were resuspended in 2 mL sterile 1× PBS and sterile filtered with 0.20 µm syringe filters (Carl Roth, Sartorius VS0271).
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bioRxiv - Immunology 2024Quote: ... The collagen suspension was mixed carefully with the cells in a ratio of 4:1 and 50 µl were added in a 96 well image lock plate (Sartorius) on ice ...
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bioRxiv - Cancer Biology 2024Quote: ... the media was changed to 90 µL of growth media containing 1:1000 Incucyte Nuclight Rapid Red Dye (Sartorius, 4717) and 1:1000 NucView 488 Caspase-3/7 substrate (Biotium ...
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bioRxiv - Developmental Biology 2020Quote: ... and LSECs were supplemented with 10µg/ml FSA-FITC (prepared as described in (47)) and 1:1000 IncuCyte® NucLight Rapid Red Reagent (cat. 4717, Sartorius). Cells were imaged hourly over a period of 24 hours using the IncuCyte Cell analysis system (Essen Bioscience) ...
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bioRxiv - Biochemistry 2022Quote: ... A template T7 with a biotin at the 5’-end annealed to the primer P2 (Table 1) was prepared by ligation and immobilized on a streptavidin-coated biosensor (SAX, Sartorius AG) at 50 nM concentration for 7 min at 500 rpm ...
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bioRxiv - Cancer Biology 2022Quote: 700 cancer and 1400 stellate cells were seeded in culture medium containing 1% FBS into the apical compartment of an Incucyte Clearview 96-well plate (Sartorius, 4582) at a 1:2 ratio ...
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Maize AFP1 confers antifungal activity by inhibiting chitin deacetylases from a broad range of fungibioRxiv - Microbiology 2021Quote: ... The 50 ml culture supernatant containing Strep-tagged CDA proteins was concentrated to 1 ml and exchanged to buffer W (100 mM Tris/HCl, 150 mM NaCl, 1 mM EDTA, pH 8) using 10 kD cutoff centrifugal filters (Sartorius, Germany) before being incubated with 25 µl of Strep-Tactin®XT agarose beads (IBA Lifesciences ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were plated at 20,000 cells/ml in Geltrex-coated 96-well plates and treated with different doses of inhibitor in the presence of 1:1000 IncuCyte® Caspase-3/7 Dye for Apoptosis (Sartorius). Doubling times were calculated by determining cell counts over multiple days with the IncuCyte Live Cell Analysis System cell-by-cell module (Sartorius).
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bioRxiv - Bioengineering 2023Quote: Sylgard-184™ silicone elastomer kit PDMS pre-polymer was well mixed (5 mins) with curing agent at a 10:1 w/w ratio using a digital balance (Sartorius BP610) prior to vacuum desiccation and casting at various temperatures (60-90°C).
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bioRxiv - Plant Biology 2023Quote: ... 250 mM imidazole) and concentrated to 1-2 mL by ultrafiltration using a Vivaspin® Turbo Centrifugal Concentrator (Sartorius, United Kingdom). To remove imidazole ...
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bioRxiv - Immunology 2023Quote: ... the mNeonGreen signals from infected MRC-5 cells were monitored for 1 week using the Incucyte™ Live-Cell analysis system (Sartorius). Additionally ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Cells were incubated for 3 hours with a red viability probe (1/3000, Incucyte® Nuclight Rapid Red Dye - Sartorius, Germany) incorporated by the nuclei of living cells ...