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Citations for Sartorius :
1 - 50 of 126 citations for Galectin 3 LGALS3 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2024Quote: ... His-tagged dROS1 protein (construct 2-3) were loaded onto penta-His sensors (Sartorius) to a response unit of about 0.5 nm followed by quenching in 10 ng/mL biocytin (Sigma-Aldrich ...
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bioRxiv - Synthetic Biology 2022Quote: ... His-tagged antigens using Anti-Penta-HIS biosensors (Sartorius/ForteBio). Antigen was loaded to a threshold of 1 nm shift ...
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bioRxiv - Immunology 2021Quote: ... S2-His-tag was immobilized on Anti-Penta-His (HIS1K) biosensors (Sartorius Corporation), and a concentration series of S2 binding mAb Fab at 200 ...
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bioRxiv - Plant Biology 2019Quote: ... The protein without His-Tag was concentrated using a Vivaspin 20 Ultrafiltration Unit (3 kDa molecular weight cutoff, Sartorius, www.sartorius.de). An appropriate volume was applied to a 1 ml loop of a gel filtration column (Superdex75 10/300 GL ...
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bioRxiv - Immunology 2021Quote: ... Anti-penta-HIS biosensors (Sartorius) were immersed in kinetics buffer (PBS + 0.5% BSA + 0.05% Tween-20 ...
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bioRxiv - Immunology 2022Quote: ... Anti-penta-HIS biosensors (Sartorius) were immersed in kinetics buffer (PBS + 0.5% BSA + 0.05% Tween-20 ...
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bioRxiv - Biochemistry 2022Quote: ... or HIS 1K biosensors (Sartorius/ForteBio) (CrossMAb ...
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bioRxiv - Biochemistry 2022Quote: Anti-His-tag (HIS1K) biosensors (Sartorius) were incubated in BLI buffer (0.002% v/v Tween-20 in 1× PBS ...
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bioRxiv - Immunology 2021Quote: ... recombinant SARS-CoV-2 S2-His-tag at 10 µg/mL was loaded on Anti-Penta-HIS (HIS1K) biosensors (Sartorius Corporation) for 1 min ...
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bioRxiv - Immunology 2021Quote: ... His-tagged ACE2 was immobilized onto Ni-NTA coated biosensors (Sartorius) before association of increasing concentrations of each RBD for 120 seconds followed by dissociation using HBS pH 7.4 for 360 seconds ...
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bioRxiv - Bioengineering 2022Quote: ... and was captured by anti-Penta-His (HIS1K) sensors (Sartorius, 18-5120). The primary antibody was diluted to 150nM with a running buffer in wells ...
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bioRxiv - Microbiology 2022Quote: ... The aliquots containing the purified His-ChuP were concentrated using a VivaSpin 6 column (Sartorius), and desalted by gel filtration in PD-10 column (GE Healthcare ...
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bioRxiv - Immunology 2021Quote: His-tagged FcαRI (Sinobiological, Cat: 10414-H08H) were immobilized via Ni-NTA biosensor (Sartorius, Cat: 18-5101) and subject to free floating IgA variants to obtained ka ...
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bioRxiv - Immunology 2021Quote: RBD-His-tag at 5 µg/mL was first loaded on Ni-NTA (NTA) biosensors (Sartorius Corporation) for 15 sec and subsequently blocked with 5 µg/mL mAbs or BLI assay buffer for 5 min ...
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bioRxiv - Bioengineering 2019Quote: ... 3 kDa MWCO concentrator (Sartorius), and the protein concentration was estimated using a BCA assay.
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bioRxiv - Bioengineering 2019Quote: ... 3 kDa MWCO concentrator (Sartorius). Protein concentrations were estimated by BCA assay.
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bioRxiv - Cell Biology 2022Quote: ... His-PKR was further concentrated to at least 10 mg/ml using Vivaspin centrifugal concentrator 30 K (Sartorius), snap-frozen in liquid nitrogen and stored at −80 °C.
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bioRxiv - Immunology 2022Quote: KD measurements of Fc-tagged Human FcμR (Cat: 13556-H02H, SinoBiological) immobilised on Anti-Human IgG Fc (AHC) (Cat: 18-5060, Sartorius) biosensors were performed on the Octet Red96® system with the loading threshold set at 1.0 nm and utilizing the recombinant Pertuzumab and Trastuzumab IgM VH whole antibodies variants in five serial diluted concentrations (12.5 to 200nM ...
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bioRxiv - Immunology 2021Quote: ... His-tagged SARS-CoV-2 RBD was prepared at 8 μg/ml in Kinetics buffer (PBS 0.01% BSA) and loaded on pre-hydrated anti-penta-HIS biosensors (Sartorius) for 2.5 min ...
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bioRxiv - Biophysics 2024Quote: ... The 50 ml containing 6-His-AdhE was concentrated to a final volume of 10.5 ml using a Vivaspin 20 centrifugal concentrator (Sartorius) with a molecular weight cut-off of 30 kDa and then dialysed against buffer A ...
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bioRxiv - Immunology 2022Quote: ... Anti-Human Fc sensor tips (ForteBio, now Sartorius) were loaded with 200 nM mAb at a threshold of 0.8nm and then submerged into wells containing 100 nM (protomer/monomer concentration ...
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bioRxiv - Biochemistry 2023Quote: ... Anti-Human Fc sensor tips (ForteBio, now Sartorius) were loaded with 200 nM mAb or FcACE2 to a threshold of 0.8 nm and then submerged into wells containing 100 nM (protomer/monomer concentration ...
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bioRxiv - Immunology 2021Quote: ... His-tagged SARS-CoV-2 RBD was prepared at 8 µg/ml and loaded on pre-hydrated anti-penta-HIS biosensors (Sartorius) for 2.5 min ...
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bioRxiv - Biochemistry 2022Quote: The specificity of the binding consisted in the immobilization of 2 μg/mL of purified VHHs on Anti-His coated sensors (His1K, Sartorius) via their His6 tag ...
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bioRxiv - Immunology 2022Quote: ... His-tagged SARS-CoV-2 RBD was prepared at 8 μg ml−1 and loaded on prehydrated anti-penta-HIS biosensors (Sartorius) for 3 min ...
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bioRxiv - Molecular Biology 2022Quote: ... concentrated to 3 ml volume (Vivaspin, Sartorius), and further purified on a HiLoad 16/600 Superdex 200 pg (Cytiva ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Caspase 3/7 indicator dye (Sartorius, Germany) and propidium iodide (BD Biosciences ...
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bioRxiv - Immunology 2023Quote: ... and Incucyte Caspase-3/7 Dye (Sartorius).
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bioRxiv - Molecular Biology 2024Quote: ... Anti-human IgG Fc capture (AHC) biosensor tips (Sartorius) were equilibrated for ten minutes in a 1:4 mixture of conditioned Freestyle 293 medium and 1X HBS EP+ to match the diluted media ...
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bioRxiv - Biochemistry 2022Quote: ... IgGs produced from the scFvs from the above sort were assessed for their competition of binding with one another using anti-Penta HIS (His1K) biosensors (Sartorius/ForteBio). His1K tips were pre-quenched with buffer containing 10nM biotin ...
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bioRxiv - Cancer Biology 2023Quote: ... The UGT2B15 promoter 20 base-pair sequence (5’-TAACTTGATTGATTTTTCCT-3’ for wild type and 5’-TAACTTGGCTGTCTTTTCCT-3’ for mutant) was immobilized to a streptavidin SADH sensor chip (Sartorius) in running buffer (10 mM HEPES pH 6.8 ...
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bioRxiv - Biochemistry 2022Quote: ... and concentrated using a 3 kDa filter (Sartorius) to remove excess dye ...
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bioRxiv - Molecular Biology 2023Quote: ... the Incucyte Caspase-3/7 Green dye (Sartorius) was added at 1:1000 dilution and live cell imaging was performed on the Incucyte S3 System (Sartorius) ...
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bioRxiv - Cancer Biology 2023Quote: ... Caspase 3/7 dye (4440, Sartorius, 1/1000) and/or propidium Iodide (PI ...
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bioRxiv - Immunology 2024Quote: ... Incucyte® Caspase-3/7 Dye (Sartorius, 4440). Antibodies ...
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bioRxiv - Immunology 2023Quote: ... Antibodies were captured on anti-human Fc AHC2 tips (Sartorius). Data capture was performed using Octet Data Acquisition software version 9.0 (ForteBio) ...
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bioRxiv - Bioengineering 2019Quote: ... together with 3 mm Ø beads (Sartorius, Goettingen, Germany), were added to Nalgene cryogenic vials (Thermo Scientific ...
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bioRxiv - Molecular Biology 2022Quote: ... a Caspase 3/7 Apoptosis reagent (Sartorius, Goettingen, Germany) was added at a final concentration of 5μM prior to imaging with phase contrast/ fluorescence (4h/imaging pattern) ...
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bioRxiv - Cancer Biology 2019Quote: ... Caspase-3/7 green apoptosis assay reagent (Sartorius #4440) was added to the cells and transferred to Incucyte® ZOOM 2FLR system and analyzed using 2016 an integrated software.
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bioRxiv - Cancer Biology 2022Quote: ... IncuCyte caspase-3/7 Red (cat#4704 from Sartorius) was added in the media ...
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bioRxiv - Molecular Biology 2022Quote: ... IncuCyte® Caspase-3/7 Apoptosis Assay Reagent (Sartorius Stedim Plastics GmbH ...
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bioRxiv - Cancer Biology 2022Quote: ... Caspase 3/7 Green Reagent (2.5 µM / well, Sartorius), Z-VAD-FMK (50 µM / well ...
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bioRxiv - Immunology 2024Quote: ... 2.5 µM Caspase-3/7 Green Dye (Sartorius, 4440) was used ...
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bioRxiv - Molecular Biology 2023Quote: Human NIL iPSCs were cultured in Nutristem (Sartorius, #05-100-1A) supplemented with Pen/Strep 0.1% on geltrex-coated plates (Thermo Fisher Scientific ...
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bioRxiv - Genetics 2023Quote: ... chip activation was made with a freshly prepared mixture of N-hydroxysuccinimide (50 mM in water) and 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (195 mM in water) for 7.5 min in DPBS (Sartorius, SKU 02-023-5A) (flow rate of 10 μL/min) ...
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bioRxiv - Cancer Biology 2024Quote: ... Caspase-3/7 Green Reagent for Apoptosis (Sartorius; cat. 4440) was added to the cell culture media ...
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bioRxiv - Cancer Biology 2021Quote: ... we used IncuCyte Caspase-3/7 Green Reagent for apoptosis (Sartorius). Crystal violet staining assay was used to assess the relative number of cells as previously described [36] ...
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bioRxiv - Microbiology 2022Quote: ... Proteins were removed by ultrafiltration (3 kDa Vivaspin; Sartorius, Göttingen, Germany). ACE and ANSA hydrolysis activities were quantified in discontinuous HPLC-based assays at 30°C in lysis buffer ...
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bioRxiv - Immunology 2022Quote: ... and quantified on the iQue 3 VBR using Forecyt software (Sartorius). Phagocytic scores were calculated by multiplying the percentage of bead positive cells by the bead fluorescence GMFI of bead positive cells and dividing by 100,000 ...
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bioRxiv - Cancer Biology 2023Quote: ... immediately followed by the addition of caspase 3/7 dye (Sartorius) at 0.5uM ...