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Citations for Sartorius :
1 - 50 of 205 citations for GATOR1 complex protein NPRL3 Nprl3 Antibody since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... The fractions containing the protein-RNA complex were concentrated to 400-700 uM with Vivaspin 5-mL centrifugal devices with 10,000 MWCO (Sartorius). Before the measurements a 10% molar excess of RNA was added to saturate the protein as well as 10% v/v D2O ...
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bioRxiv - Microbiology 2022Quote: ... The peak fractions corresponding to the protein complex were concentrated to about 16-20 mg/ml using a centrifugal filter with a PES membrane (Sartorius) and used for preparation of cryo-EM sample grids immediately.
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The eluted complex was concentrated using Vivaspin 6 Centrifugal Concentrators (Sartorius), and imidazole was removed from the protein solution by applying the sample to a PD MiniTrap Sample Column (Cytiva ...
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bioRxiv - Microbiology 2024Quote: Antibodies were loaded onto a protein A biosensor (Sartorius) for 300 s with a concentration of 10 μg/mL ...
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bioRxiv - Biochemistry 2023Quote: ... The fractions containing the complex were concentrated to 1 mg/mL using a Vivaspin centrifugal concentrator (Sartorius).
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bioRxiv - Immunology 2022Quote: BLI experiments on the IL-9 signaling complex were performed using an Octet Red 96 machine (Sartorius) in kinetics buffer (20 mM HEPES pH 7.4 ...
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bioRxiv - Biochemistry 2024Quote: ... The complex was concentrated to 4.8 mg ml-1 using a 50,000 molecular weight cut-off concentrator (Sartorius Vivaspin20). Crystallization conditions were screened manually using the hanging-drop vapor-diffusion method in 48-well plates (Hampton VDX greased ...
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bioRxiv - Molecular Biology 2023Quote: ... The monodisperse peak of the complex was collected and concentrated with a 50 kDa cut-off concentrator (Vivaspin 2, Sartorius) and subsequently used for crystallization in LCP.
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bioRxiv - Molecular Biology 2023Quote: Antibody binding to Henipavirus F proteins was assessed using BLI on an Octet RED 384 (Sartorius, formerly ForteBio) with Forte Bio Kinetics Buffer ...
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bioRxiv - Immunology 2022Quote: Binding kinetics of WS6 IgG and related S2-stem helix antibodies to diverse CoV 2P-stabilized spike proteins (S2Ps) were measured by biolayer interferometry using an Octet HTX instrument (Sartorius). All assays were performed in tilted 384-well plates (Geiger Bio-one ...
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bioRxiv - Biophysics 2024Quote: ... The protein elutes were concentrated using a protein concentrator (Sartorius #VS15T01). For protein labeling ...
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bioRxiv - Microbiology 2022Quote: The affinity between each antibody and spike protein was measured by bio-layer interferometry (BLI) with a BLItz Biolayer Interferometer System (Sartorius, Goettingen, Germany).
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bioRxiv - Biophysics 2024Quote: ... The purified proteins were then concentrated using a protein concentrator (Sartorius #VS15T01) and verified by SDS-PAGE (Fig ...
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bioRxiv - Microbiology 2024Quote: ... protein A biosensors (Sartorius) were first loaded with 10 μg/mL of mAb/bsAb in the running buffer until a binding threshold of 1 nm was reached ...
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bioRxiv - Cancer Biology 2023Quote: ... of PD-L1 protein/PD-L1 antibody was determined by Octet Biolayer interferometry (BLI) using the Octet RED384 system (Sartorius, Bohemia, NY, USA). Briefly ...
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bioRxiv - Microbiology 2024Quote: Protein–protein interactions were measured using the Octet N1 system (Sartorius, Göttingen, Germany). His-SeFtsZ and His-SeSepF were diluted in BLI buffer (20 mM Tris-HCl pH 7.4 ...
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bioRxiv - Biophysics 2024Quote: ... The Cy5-labeled tau proteins were then concentrated using a protein concentrator (Sartorius #VS15T01).
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bioRxiv - Immunology 2020Quote: ... Protein A biosensors (FortéBio/Sartorius) were first hydrated for 10 minutes in Kinetics buffer ...
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bioRxiv - Biochemistry 2023Quote: ... each Protein A biosensors (Sartorius) was pre-equilibrated in PBST buffer (PBS ...
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bioRxiv - Immunology 2022Quote: ... Protein A biosensors (Sartorius, #18-5010) were hydrated in BLI assay buffer for 10-20 minutes and loaded onto an Octet Red96 BLI instrument (Pall ...
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bioRxiv - Microbiology 2020Quote: ... For all recombinant proteins,protein-containing fractions were pooled and buffer exchange to PBS via VivaSpin columns (Sartorius) was performed ...
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bioRxiv - Neuroscience 2020Quote: ... using protein concentrator tubes (VIVASPIN 20, Sartorius). Both purified IgG fractions from patient and controls were re-tested on CBAs and the anti-IgLON5 IgG concentration was determined (titer 1:100) ...
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bioRxiv - Cancer Biology 2020Quote: Proteins were transferred to nitrocellulose membrane (Sartorius). The membranes were blocked with 10% BSA (Santa Cruz Biotechnology ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins were concentrated using centrifugal concentrator (Sartorius) and further purified with size exclusion chromatography using a Superdex 200 column (GE Life Sciences) ...
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bioRxiv - Microbiology 2023Quote: ... The eluted proteins were concentrated with a centrifugal device (MWCO 30 kDa for soluble EFNB2 proteins and 50 kDa for soluble Eph receptor proteins; Sartorius) before being separated on a Superdex 200 Increase 10/300 GL (Cytiva Life Sciences ...
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bioRxiv - Biochemistry 2020Quote: ... Proteins were concentrated using Vivaspin centrifugal filters (Sartorius) of appropriate cut-offs ...
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bioRxiv - Biochemistry 2020Quote: Purified proteins were concentrated with VIVASPIN concentrators (Sartorius) and dialyzed against E-Buffer with 1 mM DTT ...
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bioRxiv - Biochemistry 2022Quote: ... using a 100kDa MWCO Vivaspin protein concentrator (Sartorius), aliquoted ...
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bioRxiv - Biochemistry 2021Quote: ... Proteins were concentrated using Vivaspin concentrators (Sartorius Stedin) and the sample purity and homogeneity was assessed by SDS-PAGE.
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bioRxiv - Cell Biology 2024Quote: ... the protein was concentrated using Vivaspin column (Sartorius). Pellets from KCMF1-Strep expression were resuspended in 50mM Hepes/KOH pH7.5 ...
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bioRxiv - Biochemistry 2024Quote: The kinetics of the protein-protein interactions were determined using the bio-layer interferometry from the BLI system (Sartorius Octet BLI Discovery). Protein-protein interactions were observed by immobilizing 0.05 mg/ml of His-tagged VARP FL on a Ni-NTA biosensor or 0.05 mg/ml of biotinylated N-VARP on a streptavidin biosensor ...
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bioRxiv - Biophysics 2021Quote: ... Eluted protein was concentrated using centrifugal concentrators (VivaSpin, Sartorius) and then assessed for successful labeling by measuring fluorescence using the following settings and the monochromator in the BioTek Synergy Neo2 plate reader ...
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bioRxiv - Cell Biology 2023Quote: ... 2mM DTT and proteins concentrated via centrifugation (Vivaspin, Sartorius). Recombinant kinase was added to 10µl of 2X kinase buffer [40mM Hepes (pH 7.5) ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Protein was concentrated (Vivaspin 20, 50 kDa MWCO, Sartorius), and flash frozen in liquid nitrogen until further use.
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bioRxiv - Biochemistry 2024Quote: ... The protein was concentrated in a Vivaspin 20 (Sartorius) concentrator (10-kDa MWCO ...
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bioRxiv - Biochemistry 2021Quote: ... All protein samples were concentrated using Vivaspin centrifugal concentrators (Sartorius). Denaturing gel electrophoresis was performed using NuPAGE 4-12 % Bis-Tris gels (Thermo Fisher Scientific) ...
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bioRxiv - Bioengineering 2024Quote: ... the refolded protein was concentrated using a VIVAFLOW50 system (Sartorius). The concentrated sample was filtrated with 0.22 µm membrane filter ...
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bioRxiv - Biophysics 2023Quote: ... Protein samples were concentrated by ultrafiltration (5 kDa Vivaspin, Sartorius) and further purified by reverse phase chromatography using a preparative C4 column (Vydac 214 TP ...
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bioRxiv - Biophysics 2023Quote: ... Protein samples were concentrated by ultrafiltration (5 kDa Vivaspin, Sartorius) and further purified by reverse phase chromatography using a preparative C4 column (Vydac 214 TP ...
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bioRxiv - Biochemistry 2023Quote: ... The cleaved protein was then concentrated (Vivaspin 30,000 MWCO, Sartorius) and ultracentrifuged in a TLA-120.2 rotor (Beckman Coulter ...
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bioRxiv - Cancer Biology 2022Quote: ... Then proteins were transferred into ultrafiltration tubes (10kDa, Sartorius AG) and washed three times with 50 mM NH4HCO3 at 14000g for 15 minutes ...
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bioRxiv - Biophysics 2023Quote: ... Purified proteins were concentrated using centrifugal filter devices (Vivaspin, Sartorius). Protein concentration was estimated from its absorbance at 280 nm ...
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bioRxiv - Microbiology 2024Quote: ... Protein sample was concentrated with a 10K MWCO concentrator (Sartorius) and further purified by size exclusion chromatography (Superdex 200 Increase 10/300 GL GE Life Sciences).
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bioRxiv - Immunology 2024Quote: ... Experiments were performed using Octet R2 Protein Analysis System (Sartorius) and affinity was calculated using Octet Analysis Studio Software ...
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bioRxiv - Biochemistry 2024Quote: ... using a 30kDa cut-off centrifugal protein concentrator (Vivaspin, Sartorius). The final protein concentration was estimated using a microplate spectrophotometer (Multiskan SkyHigh ...
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bioRxiv - Molecular Biology 2020Quote: ... Proteins were concentrated using 30 kDa cut-off Vivaspin columns (Sartorius) and their concentration was determined by their absorbance at 280 nm.
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bioRxiv - Biochemistry 2021Quote: ... Protein was concentrated to 32 mg/ml using centrifugal concentrators (Sartorius) with a relative molecular mass cut-off of 100K.
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bioRxiv - Synthetic Biology 2021Quote: ... Protein was concentrated using a Vivaspin 500 column (Sartorius, Goettingen, Germany) column following the manufacturer’s instructions.
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bioRxiv - Immunology 2023Quote: ... All proteins were diluted in Octet kinetics buffer (Sartorius, Göttingen, Germany). Antibodies were captured on anti-human Fc AHC2 tips (Sartorius) ...
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bioRxiv - Cancer Biology 2024Quote: ... concentrated 10x using Vivaspin™ protein concentrator spin columns (Sartorius, VS15T02) and stored at 4°C.