Labshake search
Citations for Sartorius :
1 - 50 of 232 citations for Fatty acid binding protein FABP3 Human His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2024Quote: ... His-tagged dROS1 protein (construct 2-3) were loaded onto penta-His sensors (Sartorius) to a response unit of about 0.5 nm followed by quenching in 10 ng/mL biocytin (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2022Quote: The specificity of the binding consisted in the immobilization of 2 μg/mL of purified VHHs on Anti-His coated sensors (His1K, Sartorius) via their His6 tag ...
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bioRxiv - Immunology 2023Quote: ... BLI binding experiments were performed with Anti Human IgG Fc (AHC) biosensors (Sartorius 18-5060). Using Octet buffer (PBS with 0.1% Tween20) ...
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bioRxiv - Biochemistry 2022Quote: ... IgGs produced from the scFvs from the above sort were assessed for their competition of binding with one another using anti-Penta HIS (His1K) biosensors (Sartorius/ForteBio). His1K tips were pre-quenched with buffer containing 10nM biotin ...
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bioRxiv - Microbiology 2023Quote: We performed human plasma and avidity variant Abs’ binding using an Octet Red384 biolayer interferometer (Sartorius). We completed all the steps in 0.05% BSA and 0.002% Tween20 ...
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bioRxiv - Synthetic Biology 2022Quote: ... His-tagged antigens using Anti-Penta-HIS biosensors (Sartorius/ForteBio). Antigen was loaded to a threshold of 1 nm shift ...
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bioRxiv - Molecular Biology 2023Quote: Antibody binding to Henipavirus F proteins was assessed using BLI on an Octet RED 384 (Sartorius, formerly ForteBio) with Forte Bio Kinetics Buffer ...
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bioRxiv - Immunology 2021Quote: ... S2-His-tag was immobilized on Anti-Penta-His (HIS1K) biosensors (Sartorius Corporation), and a concentration series of S2 binding mAb Fab at 200 ...
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bioRxiv - Microbiology 2022Quote: ... The supernatant was sterile-filtered using 0.2µm pore size syringe filters with low protein binding (Sartorius, ref.:16534-5). The obtained FW was stored at -80°C until further analysis.
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bioRxiv - Neuroscience 2023Quote: Bio-Layer Interferometry (BLI) for compound/protein direct binding was performed using the Octet RED384 system (Sartorius BioAnalytical Instruments) and biotinylated SYK-tSH2 protein (SYKA-c022 cleaved/biotinylated ...
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bioRxiv - Immunology 2021Quote: ... Anti-penta-HIS biosensors (Sartorius) were immersed in kinetics buffer (PBS + 0.5% BSA + 0.05% Tween-20 ...
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bioRxiv - Immunology 2022Quote: ... Anti-penta-HIS biosensors (Sartorius) were immersed in kinetics buffer (PBS + 0.5% BSA + 0.05% Tween-20 ...
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bioRxiv - Plant Biology 2019Quote: ... The protein without His-Tag was concentrated using a Vivaspin 20 Ultrafiltration Unit (3 kDa molecular weight cutoff, Sartorius, www.sartorius.de). An appropriate volume was applied to a 1 ml loop of a gel filtration column (Superdex75 10/300 GL ...
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bioRxiv - Biochemistry 2022Quote: ... or HIS 1K biosensors (Sartorius/ForteBio) (CrossMAb ...
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bioRxiv - Biochemistry 2022Quote: Anti-His-tag (HIS1K) biosensors (Sartorius) were incubated in BLI buffer (0.002% v/v Tween-20 in 1× PBS ...
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bioRxiv - Immunology 2021Quote: ... recombinant SARS-CoV-2 S2-His-tag at 10 µg/mL was loaded on Anti-Penta-HIS (HIS1K) biosensors (Sartorius Corporation) for 1 min ...
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bioRxiv - Immunology 2022Quote: The binding kinetic was performed using Octet RED96E (Sartorius). SAX sensors were loaded with biotinylated ACE2 25μg/ml and subsequently quenched with biocytin ...
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bioRxiv - Biochemistry 2023Quote: BLI binding assays were performed on an Octet Red (Sartorius) instrument operated at 30℃ with shaking (1000 rpm) ...
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bioRxiv - Biochemistry 2024Quote: BLI binding assays were performed on an Octet Red (Sartorius) instrument operated at 30°C with shaking (1000 rpm) ...
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bioRxiv - Bioengineering 2022Quote: ... Binding reactions were performed in 1X kinetic buffer (Sartorius, 18-1105) consisting of 20 mM phosphate buffer ...
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bioRxiv - Biochemistry 2023Quote: Binding assays were carried out on an Octet RED96 instrument (Sartorius) using a previously established protocol (He et al. ...
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bioRxiv - Molecular Biology 2023Quote: ... The labeled tip was then dipped in 1x binding buffer (Sartorius) for 60 seconds to remove excess ligand and achieve baseline ...
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bioRxiv - Synthetic Biology 2023Quote: Biolayer interferometry binding data were collected on an Octet R8 (Sartorius) and processed using the instrument’s integrated software ...
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bioRxiv - Immunology 2023Quote: Binding by BLI was performed using an Octet K2 system (Sartorius) using anti-human IgG-Fc biosensors (AHC ...
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bioRxiv - Immunology 2021Quote: ... His-tagged ACE2 was immobilized onto Ni-NTA coated biosensors (Sartorius) before association of increasing concentrations of each RBD for 120 seconds followed by dissociation using HBS pH 7.4 for 360 seconds ...
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bioRxiv - Biophysics 2022Quote: ... The binding kinetics were analyzed using the Octet DataAnalysis 10.0 software (Sartorius) to deduce the kinetic parameters ...
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bioRxiv - Bioengineering 2022Quote: ... and was captured by anti-Penta-His (HIS1K) sensors (Sartorius, 18-5120). The primary antibody was diluted to 150nM with a running buffer in wells ...
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bioRxiv - Synthetic Biology 2023Quote: ... IgGs were assessed for binding to biotinylated antigens using streptavidin biosensors (Sartorius/ForteBio). Antigen was loaded at a concentration of 200nM ...
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bioRxiv - Biochemistry 2022Quote: Analysis of binding kinetics was done at 23 °C on Octet K2 (Sartorius AG). A template T7 with a biotin at the 5’-end annealed to the primer P2 (Table 1 ...
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bioRxiv - Immunology 2022Quote: Binding kinetics were measured using an Octet RED96 Biolayer Interferometer (Sartorius ForteBio, Freemont, CA)30 ...
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bioRxiv - Cell Biology 2023Quote: Binding kinetics analysis of Drp1 Nbs was performed using the Octet RED96e system (Sartorius) according to the manufacturer’s recommendations ...
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bioRxiv - Biochemistry 2024Quote: Analysis of binding kinetics was done at 23 °C using Octet K2 (Sartorius AG), as previously described (61) ...
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bioRxiv - Biochemistry 2022Quote: Analysis of binding kinetics was done at 23 ºC on an Octet K2 (Sartorius AG). This device uses Bio-Layer Interferometry technology to monitor molecular interactions in real time ...
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bioRxiv - Biochemistry 2020Quote: ... followed by concentrating and buffer exchange into IMAC binding buffer using Vivaflow 100 system (Sartorius). The resulting protein solution was purified by Histrap column (GE Healthcare ...
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bioRxiv - Immunology 2023Quote: ... BLI ligand titration and binding kinetics assays were performed on an Octet Red96e system (Sartorius) at 30°C with an orbital shake speed of 1000 rpm ...
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bioRxiv - Immunology 2024Quote: ... Binding was read by flow cytometry on an IntelliCyte High Throughput Flow Cytometry (IntelliCyte/Sartorius).
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bioRxiv - Microbiology 2022Quote: ... The aliquots containing the purified His-ChuP were concentrated using a VivaSpin 6 column (Sartorius), and desalted by gel filtration in PD-10 column (GE Healthcare ...
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bioRxiv - Immunology 2022Quote: The binding affinity of obtained antibodies to RBD was examined by the Blitz system (Sartorius Japan) using protein A-coated biosensors ...
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bioRxiv - Cell Biology 2021Quote: Analysis of binding kinetics of Miro1-specific Nbs was performed using the Octet RED96e system (Sartorius) according to the manufacturer’s recommendations ...
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bioRxiv - Biochemistry 2022Quote: Competition binding assays were performed by a premix method with streptavidin bio-sensor (SA sensor, Sartorius). Firstly ...
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bioRxiv - Synthetic Biology 2022Quote: The binding kinetics of antibodies to RBD was measured by BLI on an Octet-R2 (Sartorius). The his-tagged RBD variants were loaded onto Ni-NTA biosensors from 10 ug\ml solution in kinetic buffer (PBS ...
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bioRxiv - Synthetic Biology 2022Quote: ... IgGs and Fabs were assessed for binding to biotinylated antigens using streptavidin (SA) biosensors (Sartorius/ForteBio) or to unbiotinylated ...
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bioRxiv - Immunology 2023Quote: Analysis of binding kinetics of hSIRPα specific Nbs was performed using the Octet RED96e system (Sartorius) as per the manufacturer’s recommendations ...
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bioRxiv - Molecular Biology 2023Quote: ... The IgG loaded biosensor tips then were submerged in binding buffer (kinetics buffer (Sartorius #18-1105) in PBS ...
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bioRxiv - Cancer Biology 2023Quote: ... the FcRn binding assay was measured in a biolayer interferometry assay on an Octet RED96 system (Sartorius). Streptavidin-coated biosensors were loaded with purified biotinylated human FcRn in pH 6.0 assay buffer (100mM sodium phosphate ...
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bioRxiv - Biophysics 2024Quote: ... The acquired raw data for the binding of anti-TcPOP mAbs with TcPOP were processed and globally fit to a 1:1 binding model with Octet Analysis Studio 12.2.2.26 Software (Sartorius). The binding kinetics measurements were carried out in three replicates ...
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bioRxiv - Immunology 2021Quote: His-tagged FcαRI (Sinobiological, Cat: 10414-H08H) were immobilized via Ni-NTA biosensor (Sartorius, Cat: 18-5101) and subject to free floating IgA variants to obtained ka ...
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bioRxiv - Immunology 2021Quote: RBD-His-tag at 5 µg/mL was first loaded on Ni-NTA (NTA) biosensors (Sartorius Corporation) for 15 sec and subsequently blocked with 5 µg/mL mAbs or BLI assay buffer for 5 min ...
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bioRxiv - Microbiology 2022Quote: Binding kinetics measurements were characterized using biolayer interferometry (BLI) measurements on an Octet QKe instrument (Sartorius, Göttingen, Germany), as previously described 66 ...
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bioRxiv - Cancer Biology 2023Quote: ... The binding affinity between HER3-ECD and LRG1 or neuregulins was measured by the Octet RED96 system (Sartorius), at 25 °C in 1X PBS buffer ...