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Citations for Sartorius :
201 - 250 of 256 citations for Dengue Virus Serotype 3 Envelope Protein HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... CV30 or CR3022 IgG at 10ug/mL was loaded onto pre-hydrated Protein A biosensors (Pall FortéBio/Sartorius) for 150 s followed by a 60 s baseline ...
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bioRxiv - Synthetic Biology 2021Quote: ... and 30 μg of total protein was loaded into 30-kDa-cutoff Vivacon 500 ultrafiltration spin columns (Sartorius). Samples were digested for 4 h on a filter with 1:50 Lys-C (Wako ...
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bioRxiv - Microbiology 2023Quote: ... Protein-containing fractions were pooled and concentrated to 10 mg/ml using 10 kDa MWCO spin concentrators (Sartorius). Protein purity and identity were confirmed by detection of a single band by Coomassie-stained SDS-PAGE and by LC-MS/MS to confirm the expected sequence.
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bioRxiv - Molecular Biology 2023Quote: Antibody binding to Henipavirus F proteins was assessed using BLI on an Octet RED 384 (Sartorius, formerly ForteBio) with Forte Bio Kinetics Buffer ...
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bioRxiv - Microbiology 2023Quote: ... Purified protein fractions were dialyzed against 1X PBS (pH 7.4) using the Vivaspin 10 kDa centrifuge system (Sartorius). Protein quantification was performed in a spectrophotometer at 280 nm and the assays were performed with the enzyme at a concentration of 5.88 μM.
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bioRxiv - Cell Biology 2023Quote: ... Cleaved protein was eluted in fresh cleavage buffer and concentrated to 0.5 mL using a centrifugal concentrator (Sartorius). Protein concentration and quality was determined by NanoDrop® ND-1000 spectrophotometer (Labtech ...
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bioRxiv - Immunology 2023Quote: ... and 37.7H IgG were diluted to concentrations of 10 µg/mL and loaded onto Protein A sensors (Sartorius) to a binding signal of 1.0 nM ...
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bioRxiv - Bioengineering 2023Quote: ... was washed three times in DI water and concentrated to a final volume of 1 mL using 3 kDa MWCO ultrafiltration tubes (Sartorius Stedim Biotech, Germany). The tubes were spun at 4000 rpm for 40 minutes ...
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bioRxiv - Bioengineering 2024Quote: ... The purified protein from elutions containing the fewest contaminating proteins (typically elution steps 3-5) were concentrated in 1× PBS using a 5 kDa cut-off Vivaspin Centrifugal Concentrator (Sartorius, Oakville, Ontario, Canada). Protein concentrations were determined using the micro-BCA assay (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2021Quote: ... The desalted fraction of SpyCas9 protein was concentrated by using Vivaspin turbo 15 concentrators (50,000 MWCO; Sartorius, Goettingen, Germany). The concentrated protein was quantified by Bradford assay and was kept at −80 °C.
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bioRxiv - Biochemistry 2020Quote: ... Pure protein was concentrated to 40 mg/mL with Vivaspin 20 (MWCO 3000) ultrafiltration units (Sartorius AG, Göttingen, Germany) and concentration was determined photometrically ...
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bioRxiv - Cell Biology 2019Quote: Protein samples were prepared using filter-aided sample preparation (FASP) digestion (Wisniewski et al., 2009) (Vicacon500, Sartorius, VN01H02 10kDA). The FASP filter were washed with 200 µl 0.1 % trifluoroacetic acid (TFA ...
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bioRxiv - Molecular Biology 2021Quote: ... The protein was then concentrated to 0.5 ml using a 50 kDa cutoff concentrator (Vivaspin, Sartorius, MWCO 50 kDa).
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bioRxiv - Molecular Biology 2021Quote: ... The fractions containing the protein-RNA complex were concentrated to 400-700 uM with Vivaspin 5-mL centrifugal devices with 10,000 MWCO (Sartorius). Before the measurements a 10% molar excess of RNA was added to saturate the protein as well as 10% v/v D2O ...
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bioRxiv - Microbiology 2022Quote: ... The supernatant was sterile-filtered using 0.2µm pore size syringe filters with low protein binding (Sartorius, ref.:16534-5). The obtained FW was stored at -80°C until further analysis.
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bioRxiv - Microbiology 2023Quote: ... Proteins were spotted in duplicate onto a nitrocellulose 64 pad coated UniSart glass slide (Sartorius Stedim Biotech, Goettingen, Germany) or 24 pad coated AVID glass slide (Grace Bio-Labs ...
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bioRxiv - Immunology 2023Quote: ... Anti-HA antibodies were diluted in kinetics buffer (PBS with 0.5% serum bovine albumin and 0.01% Tween) to a final concentration of 10 μg/mL before loading onto protein A biosensors (Sartorius) for 200 s ...
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bioRxiv - Microbiology 2023Quote: All eluted proteins were subsequently buffer exchanged to PBS or TBS by using Vivaspin20 ultrafiltration units (Sartorius, Gӧttingen, Germany) with MWCO 50 kDa (Env ...
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bioRxiv - Neuroscience 2023Quote: Bio-Layer Interferometry (BLI) for compound/protein direct binding was performed using the Octet RED384 system (Sartorius BioAnalytical Instruments) and biotinylated SYK-tSH2 protein (SYKA-c022 cleaved/biotinylated ...
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bioRxiv - Immunology 2023Quote: ... Anti-HA antibodies were diluted in kinetics buffer (PBS with 0.5% serum bovine albumin and 0.01% Tween) to a final concentration of 10 μg/mL before loading onto protein A biosensors (Sartorius) for 200 s ...
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bioRxiv - Biochemistry 2023Quote: ... The resulting protein was concentrated to ≈4 mg/mL using a 5 kDa centrifugal concentrator (Vivaspin; Sartorius, Gottingen, Germany). For drop-deposition Raman spectroscopy ...
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bioRxiv - Immunology 2022Quote: ... 200 nM of randomly biotinylated SARS-CoV-2 S or RBD protein antigen was captured using SA biosensors (18-5019, Sartorius). The biosensor was loaded with antigen for 5 min and then moved into the saturating mAbs at a concentration of 100 µg/mL for 10 min ...
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bioRxiv - Immunology 2022Quote: Binding kinetics of WS6 IgG and related S2-stem helix antibodies to diverse CoV 2P-stabilized spike proteins (S2Ps) were measured by biolayer interferometry using an Octet HTX instrument (Sartorius). All assays were performed in tilted 384-well plates (Geiger Bio-one ...
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bioRxiv - Biochemistry 2020Quote: ... glycerol was added and the protein concentrated to 20 – 30 mg/mL in a 10 kDa MWCO spin concentrator (Vivaspin, Sartorius).
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bioRxiv - Biochemistry 2021Quote: The dialysed proteins were concentrated to a <5 mL volume using a 30 kDa MWCO Vivaspin 20 centrifugal concentrator (Sartorius) at 5000 g ...
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bioRxiv - Microbiology 2020Quote: ... The protein was loaded on a HiLoad 16/600 Superdex 200 pg column pre-equilibrated with storage buffer and the fractions containing pure Rel protein were collected and concentrated in a Vivaspin turbo 15 (Sartorius). The purity of protein preparations and the absence of RNA were assessed by SDS-PAGE and on a 1% agarose gel (in 1 × TAE buffer ...
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bioRxiv - Molecular Biology 2021Quote: ... the clarified supernatant was loaded onto Vivaspin protein concentrator columns with a molecular weight cut-off of 30 kDa (Sartorius) and centrifuged at 4,000 x g ...
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bioRxiv - Microbiology 2020Quote: ... Protein samples were quantified by BCA assay (Pierce) and transferred to a 10 kDa MWCO spin filter (Vivaspin 500; Sartorius) before centrifugation at 12,000 x g to collect denatured and reduced proteins atop the filter membrane ...
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bioRxiv - Immunology 2021Quote: ... Purified S proteins were exchanged to storage buffer and then filtered through 0.22 um filter (Sartorius Stedim Biotech, Göttingen, Germany). The concentration of purified protein was determined by ELISA ...
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bioRxiv - Molecular Biology 2021Quote: ... the clarified supernatant was loaded onto Vivaspin protein concentrator columns with a molecular weight cut-off of 30 kDa (Sartorius) and centrifuged at 4,000 x g ...
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bioRxiv - Biochemistry 2020Quote: ... The protein was expressed and purified as previously described.26 The purified protein was concentrated to 10-20 mg/mL using a Vivaspin15R (Sartorius), and purity was estimated to be at least 95% by SDS-PAGE.
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bioRxiv - Biochemistry 2021Quote: ... The protein solution was concentrated to 10 – 20 mg/mL using a Centrisart I MWCO 100 kDa concentrator (Sartorius AG).
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bioRxiv - Immunology 2022Quote: KD measurements of the Pertuzumab and Trastuzumab IgMs to their Her2 antigen (Cat: 10004-HCCH, SinoBiological) were performed by first immobilizing them on Protein L (Cat: 18-5085, Sartorius) and CaptureSelect™ Biotin Anti-IgM Conjugate (Cat ...
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bioRxiv - Microbiology 2022Quote: ... The peak fractions corresponding to the protein complex were concentrated to about 16-20 mg/ml using a centrifugal filter with a PES membrane (Sartorius) and used for preparation of cryo-EM sample grids immediately.
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bioRxiv - Systems Biology 2022Quote: ... live-cell proliferation and expression of YFP-tagged proteins were tracked for 48 h using an Incucyte S3 device (Sartorius).
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bioRxiv - Cancer Biology 2023Quote: ... fractions containing the protein of interest were pooled together and concentrated using Vivaspin 20 centrifugal concentrators with a 5 kDa cut-off (Sartorius). The concentration of BRD4 BD1 was determined spectrophotometrically at 280 nm using the calculated molar extinction coefficient ...
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bioRxiv - Biophysics 2023Quote: ... The flow-through volume containing the untagged protein was concentrated to 10 mg/mL using Vivacon500 filters (50 kDa, Sartorius) for further purification using a Superdex 200 Increase 5/150 GL column equilibrated in 50 mM Tris-HCl pH 7.0 ...
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bioRxiv - Cell Biology 2020Quote: ... 5 mM dithiothreitol (DTT)) at 4°C overnight and concentrated to ~3-5 mg/mL using Vivaspin 6 concentrators (Sartorius, 6 ml, 100 kDa cut-off). Mammalian septin hexamers composed of mouse Sept2 (98.6% identical to human Sept2 ...
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bioRxiv - Microbiology 2019Quote: ... Each mixture containing 100μg proteins was loaded onto a 10 kDa cut-off spin column (Vivacon 500, Sartorius AG, Goettingen, Germany). The lysate was adjusted to 8M urea by centrifuging to remove SDS and low-molecular-weight material ...
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bioRxiv - Biochemistry 2021Quote: ... The protein was concentrated to 25-30 µM with the use of a Centrisart I MWCO 100 kDa concentrator (Sartorius AG) and passed through a spin-out column of Bio-Gel P-6 equilibrated with the protein storage buffer (0.1 M Hepes-HCl ...
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bioRxiv - Cancer Biology 2024Quote: ... Glioma cells were stably transduced with a lentiviral nuclear red fluorescent protein under the EF1a promotor (Sartorius, cat. 4476, Göttingen, Germany) for visualization in live imaging assays ...
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bioRxiv - Microbiology 2019Quote: ... adapted by us to the use of 100 µg protein for digestion with sequencing-grade trypsin in 50:1 ratios in Vivacon 10k filters (Sartorius AG, Germany) (28) ...
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bioRxiv - Immunology 2019Quote: ... Worm culture supernatants collected over 1 week were further concentrated using centrifugal protein concentrators with a 5 kDa MWCO (Vivaspin, Sartorius, Göttingen, Germany) to obtain the final ...
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bioRxiv - Biochemistry 2020Quote: ... bengalensis protein hydrolysates (BBPH) were subjected to centrifugal ultrafiltration using 3kDa cut-off membrane filtration unit (Vivaspin20, VS2091, Sartorius AG, Goettingen, Germany) to separate the low molecular peptide fraction (< 3kDa) ...
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bioRxiv - Microbiology 2022Quote: The affinity between each antibody and spike protein was measured by bio-layer interferometry (BLI) with a BLItz Biolayer Interferometer System (Sartorius, Goettingen, Germany).
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bioRxiv - Plant Biology 2023Quote: The biolayer interferometry was performed as previously described (Xie et al., 2022) using Octet® R2 Protein Analysis System (Sartorius, Figure 1) or Gator-plus (Gator Bio ...
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bioRxiv - Plant Biology 2023Quote: ... Finally, protein concentration and buffer exchange (20 mM Tris-HCl, pH 7.5) was performed using Vivaspin 30K columns (Sartorius, Germany, Göttingen, Germany).
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bioRxiv - Biophysics 2023Quote: ... proteins were exchanged to labelling buffer (20 mM HEPES, 50 mM NaCl, pH 7.5) using VivaSpin 6 concentrator (Sartorius AG, Gottingen, Germany).
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bioRxiv - Bioengineering 2022Quote: ... and HT29 cells were transfected to express the nuclear-restricted mKate2 red fluorescent protein using the IncuCyte® NucLight Lentivirus Reagents (Sartorius, Cat: 4476) and following the manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: ... Protein production at pilot scale for nonclinical trial material was archived in 500L Bioreactor (BIOSTAT STR®500, Sartorius Stedim Biotech, Göttingen, Germany) using a fed-batch process using PowerCHO-2 medium as basal medium ...