Labshake search
Citations for Sartorius :
1 - 50 of 91 citations for 8 Chloro 4 ethoxyquinoline since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cancer Biology 2020Quote: ... were homogenized in liquid nitrogen pre-cooled 4 ml PTFE-vials with one 8 mm stainless steel bead using a Micro-Dismembranator (Sartorius AG, Göttingen) at 2600 RPM for two minutes ...
-
bioRxiv - Immunology 2023Quote: ... a RED96E 8-Channel System (Sartorius Group, Gottingen, Germany). Determination of neutralizing capacities by the FO-BLI biosensor was completed automatically within ten minutes.
-
bioRxiv - Cancer Biology 2024Quote: ... Initial data processing was performed using the ForeCyt 8 software (Sartorius). The AUC of viability and differentiation were calculated with in-house R scripts ...
-
bioRxiv - Neuroscience 2024Quote: ... Lysates were spun at 15,000g for 5 min at 4°C and supernatants were passed through a 4 mm regenerated cellulose filter (4 mm Ø, Sartorius). Samples were then dried under nitrogen flow at 40°C and resuspended in 125 µl of methanol/water 70:30 for subsequent analyses.
-
bioRxiv - Molecular Biology 2023Quote: ... with a CIM quaternary amine (QA) 8 mL column (Sartorius BIA separations, Slovenia), and with step gradient elution to a final elution volume of 25 mL ...
-
bioRxiv - Microbiology 2021Quote: ... pooled and concentrated to 7-8 mg/ml using Vivaspin 20 concentrators (30 kDa MWCO, Sartorius). Small aliquots were flash-frozen in liquid nitrogen and stored at −80°C ...
-
bioRxiv - Cell Biology 2019Quote: ... 4°C in Vivaspin20 columns (Cat.#VS2032,Sartorius) and stored at –80°C.
-
bioRxiv - Bioengineering 2020Quote: ... The AAV2/8 was purified by affinity chromatography and concentrated using ultrafiltration columns (Sartorius Stedim Biotech, Goettingen, Germany), washed in PBS and concentrated to a volume of 100–150 µl ...
-
bioRxiv - Immunology 2021Quote: ... His-tagged SARS-CoV-2 RBD was prepared at 8 μg/ml in Kinetics buffer (PBS 0.01% BSA) and loaded on pre-hydrated anti-penta-HIS biosensors (Sartorius) for 2.5 min ...
-
bioRxiv - Cancer Biology 2022Quote: ... The proteins were resuspended in 200 μL 8 M urea (pH 8.0) and loaded in 30 kD Microcon filter tubes (Sartorius) and centrifuged at 12,000 g for 20 min ...
-
bioRxiv - Bioengineering 2022Quote: ... ACM was added and centrifuged in a swing-bucket centrifuge for 8 minutes at 4,000 g per the recommendation by Sartorius. The column was inverted ...
-
bioRxiv - Immunology 2021Quote: ... His-tagged SARS-CoV-2 RBD was prepared at 8 µg/ml and loaded on pre-hydrated anti-penta-HIS biosensors (Sartorius) for 2.5 min ...
-
bioRxiv - Immunology 2022Quote: ... His-tagged SARS-CoV-2 RBD was prepared at 8 μg ml−1 and loaded on prehydrated anti-penta-HIS biosensors (Sartorius) for 3 min ...
-
bioRxiv - Microbiology 2023Quote: ... Final separation was achieved following concentrating the Heparin elution to approximately 8 mg/mL using a 30 kDa MWCO Vivaspin filter (Sartorius), by applying the sample to a Superdex 200 10/300 GL (Cytiva ...
-
bioRxiv - Physiology 2024Quote: ... Cultures were monitored for colony formation from 2 to 8 weeks after plating using Incucyte live cell imaging system (Sartorius). Wells with single colonies were harvested and cell cultures were expanded for making stocks and for DNA isolation ...
-
bioRxiv - Cancer Biology 2023Quote: ... 2.5 x 103 cells/well were plated in FBS-free media in the upper chamber of a 96-well Incucyte ClearView cell migration plate (8 µm pore size; #4582 Sartorius). Cells were incubated at 37°C for 45 minutes to allow cells to settle on the membrane before adding RPMI-1640 media supplemented with 10% FBS to the bottom chamber ...
-
bioRxiv - Developmental Biology 2024Quote: ... the organs on scales accurate to 4 decimal places (Sartorius BP61S). Glucose levels were obtained using a One-Touch ULTRA (Lifescan ...
-
Maize AFP1 confers antifungal activity by inhibiting chitin deacetylases from a broad range of fungibioRxiv - Microbiology 2021Quote: ... The 50 ml culture supernatant containing Strep-tagged CDA proteins was concentrated to 1 ml and exchanged to buffer W (100 mM Tris/HCl, 150 mM NaCl, 1 mM EDTA, pH 8) using 10 kD cutoff centrifugal filters (Sartorius, Germany) before being incubated with 25 µl of Strep-Tactin®XT agarose beads (IBA Lifesciences ...
-
bioRxiv - Cancer Biology 2023Quote: ... 25 µl of single-cell suspension was dispensed on ready-to-use plates with an 8-channel electronic Picus® pipette (10 to 300 µl, #735361, Sartorius).
-
bioRxiv - Physiology 2019Quote: ... were inoculated with 2 mL of stock culture and grown at 30°C and at 200 rpm for 8 h in a B Braun Certomat BS-1 incubator (Sartorius, Melsungen, Germany). Bioreactor batch cultures were started by transferring 400 mL of preculture to a bioreactor containing 4.6 L of medium ...
-
bioRxiv - Microbiology 2019Quote: ... 300 mM ammonium acetate pH 7.5 (SpaOC/SpaO/OrgB) or 50 mM ammonium acetate pH 8 (SpaO/SpaOC/OrgB/InvC) using Vivaspin® 500 centrifugal concentrators (Sartorius, Göttingen, Germany). SEC-purified proteins were used for all samples but the SpaO/SpaOC/OrgB complex ...
-
bioRxiv - Cell Biology 2022Quote: ... concentrated in 1 mL in a Vivaspin Turbo 4 (30’000 MWCO, Sartorius VS04T22) and further purified on a Superdex 75 10/300 gel filtration column (GE Healthcare).
-
bioRxiv - Microbiology 2021Quote: ... 4°C) and the supernatant was passed through a 0.45 µm syringe filter (Sartorius). StrepII-CERT was captured using 1 mL StrepTrap HP column (GE Healthcare ...
-
bioRxiv - Cell Biology 2020Quote: ... the protein was simultaneously concentrated and buffer-exchanged (with a Vivaspin Turbo 4; Sartorius) into PM buffer (80 mM Pipes pH 6.8 ...
-
bioRxiv - Developmental Biology 2024Quote: ... and weights obtained using a fine balance accurate to 4 decimal places (Sartorius BP61S). Paired organs (lungs and kidneys ...
-
bioRxiv - Developmental Biology 2024Quote: ... and weights obtained using a fine balance accurate to 4 decimal places (Sartorius BP61S). Paired organs (lungs and kidneys ...
-
bioRxiv - Cell Biology 2020Quote: A549 lung cancer cells (4*104) were plated in a 96-well ImageLock plate (Sartorius) and cultured in a 20% O2 ...
-
bioRxiv - Biochemistry 2021Quote: ... concentrated to 1 mL using a Vivaspin 4 Turbo concentrator (Cut-off 5 kDa; Sartorius), and purified via size-exclusion chromatography with a Superdex S75-10/300 column (GE Healthcare).
-
bioRxiv - Molecular Biology 2023Quote: ... concentrated by centrifugation at 4,000xg at 4°C through VivaSpin 10 kDa MWCO concentrators (Sartorius), aliquotted ...
-
bioRxiv - Animal Behavior and Cognition 2022Quote: ... and again weighed at the end of the treatment after 4 h (Sartorius ME36S Balance) to confirm that starved larvae lost more body mass than control larvae (Supplement S1) ...
-
bioRxiv - Cancer Biology 2023Quote: ... Cells were imaged every 4 hours using the IncuCyte S3 live-cell analysis system (Sartorius). Confluence data was analyzed using the area under the curve (AUC ...
-
bioRxiv - Cell Biology 2022Quote: ... Protein was concentrated from the media using VivaSpin Turbo 4 (10000 MWCO) concentrator tubes (Sartorius, VS04T01). Protein samples were run on 4-15% Mini protean TGX stain-free gels (Bio-Rad ...
-
bioRxiv - Microbiology 2019Quote: ... Peak fractions were pooled and concentrated to ∼30 mg/mL using a Vivaspin 4 Turbo concentrator (Sartorius).
-
bioRxiv - Microbiology 2020Quote: ... and diplopterol (Chiron AS) were dissolved in acetonitrile and filtered with Minisart RC 4 (Sartorius, Stonehouse, UK) before applying 5 μl aliquots to a Acquity UPLC BEH C18 column (1.7 μm ...
-
bioRxiv - Microbiology 2020Quote: ... Eluted total IgG was concentrated by centrifugation at 4°C for 30 mins using 100000MWCO vivaspin (Sartorius). The concentrate was then dialyzed using a dialysis tube (Millipore ...
-
bioRxiv - Biophysics 2022Quote: ... Dialyzed protein was then concentrated with 10 kDa MWCO Vivaspin centrifugal concentrators (3,500 xg, 4°C) (Sartorius). Protein concentrations were determined by means of fluorescence using an sfGFP calibration curve ...
-
bioRxiv - Biochemistry 2023Quote: ... Samples were then diafiltered at 4°C using 3 kDa cutoff VivaSpin®500 centrifuge filters (Sartorius) for 3 cycles of 20 min at 14,000 rpm ...
-
bioRxiv - Cancer Biology 2022Quote: ... Four images were acquired per well every 4 hours and analyzed by the IncuCyte 2020B software (Sartorius) to determine cell confluence ...
-
bioRxiv - Microbiology 2023Quote: ... which took place overnight on arrival at 4°C (cold room) using 6 Vivaflow200 filtration units (Sartorius) operating in two parallel series ...
-
bioRxiv - Biophysics 2021Quote: ... at pH 7 via centrifugal filter units at 12000 g at 4°C (Vivaspin 500, MWCO 10000, Sartorius). Glycans for native MS analysis were mixed with 1 µM (per monomer ...
-
bioRxiv - Cancer Biology 2021Quote: ... We imaged cells every 6 hours for 4-6 days of treatment using Incucyte Zoom (Sartorius, Michigan, USA). Fluorescence was quantified using four locations in each treated sample by the IncuCyte Zoom 2016B software.
-
bioRxiv - Cancer Biology 2023Quote: ... cell confluency was determined every 4 hours using live cell imaging system Incucyte® S3 (Sartorius, Göttingen, Germany) and plotted relative to initial values.
-
bioRxiv - Biochemistry 2023Quote: ... Obtained EHEP was then concentrated to 15–25 mg/mL using Vivaspin-4 10K columns (Sartorius, Göttingen, Germany). About akuBGL ...
-
bioRxiv - Neuroscience 2023Quote: ... The dialyzed solution was finally concentrated by centrifugation through a Vivaspin Turbo 4 membrane filter (MWCO 10,000; Sartorius) at 3,500 × g for 30-45 min at 4°C.
-
bioRxiv - Biochemistry 2020Quote: ... oxidized BdiGolSase1 was ultrafiltrated by centrifuging at 10,000 x g and 4°C using a Vivaspin 500 device (Sartorius). To evaluate the reversibility of the process ...
-
bioRxiv - Biochemistry 2021Quote: ... appropriate fractions (selected by 4-20% gradient SDS-PAGE) were pooled and concentrated by centrifugal filtration (Vivaspin 20, 5,000 MWCO PES, Sartorius) for loading onto a Superdex 200 Increase 10/300 GL (GE Healthcare ...
-
bioRxiv - Biophysics 2020Quote: ... 250 mM) at pH 7.5 and 4°C via centrifugal filter units (13000 x g, Vivaspin 500, MWCO 10000 (Sartorius) or Micro Bio-Spin 6 columns (Bio-Rad) ...
-
bioRxiv - Molecular Biology 2022Quote: ... were concentrated to 2 ml by centrifugation 30 min at 4,000xg at 4°C through a 20 ml VivaSpin 20 30 kDa MWCO concentrator (Sartorius, and were diluted with TGE containing 1 mM β-mercaptoethanol to adjust the NaCl concentration to 50 mM ...
-
bioRxiv - Plant Biology 2022Quote: ... The supernatant was filtered with Minisart RC4 (pore size 0.20 µm, diameter 4 mm, Sartorius stedim biotech, Göttingen, Germany) and injected into a high-performance liquid chromatography system (LC-10AD ...
-
bioRxiv - Microbiology 2023Quote: ... Supernatants were thawed and filtrated through a 0.2 µm filter before centrifugation at 4500 x g at 4°C in VivaSpin tubes (Sartorius). The first-generation immunoadhesins do not carry the Twin-Strep-tag and were purified using Protein A Mag Sepharose™ Xtra beads (50 µL beads/mL of supernatant ...