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Citations for Sartorius :
101 - 150 of 213 citations for 7 chloro 4 oxo 4H chromene 2 carboxylic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... The lysate was cleared by centrifugation at 35,000 rcf for 45 minutes at 4°C followed by filtering with a GF filter (Sartorius). The cleared lysate was incubated with 2 mL Ni-NTA agarose (Qiagen ...
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bioRxiv - Microbiology 2024Quote: ... The lysate was cleared by centrifugation at 70,000 rcf for 45 minutes at 4°C followed by filtering with a GF filter (Sartorius). The cleared lysate was incubated with 4 mL of IgG beads (Cytiva ...
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bioRxiv - Microbiology 2024Quote: ... Lysates were cleared by centrifugation at 35,000 rcf for 45 minutes at 4°C followed by filtering with a GF filter (Sartorius). Cleared lysates were then incubated with 1.5 mL of StrepTactin Sepharose beads (IBA Lifesciences ...
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bioRxiv - Microbiology 2024Quote: ... Lysates were cleared by centrifugation at 35,000 rcf for 30 minutes at 4°C followed by filtering with a GF filter (Sartorius). For purification of ScaC and ScaC-SNAP ...
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bioRxiv - Evolutionary Biology 2024Quote: ... approximately 4 mg of pulverized tissue was weighed using an ultra-micro balance (CP2P Microbalance, Sartorius GmbH, Goettingen, Germany), packaged into tin capsules ...
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bioRxiv - Cancer Biology 2019Quote: ... Two peaks were collected and concentrated on a 2 mL Vivaspin 10 MWCO (Sartorius, UK). The molecular sizes of peak 1 (300 kDa ...
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bioRxiv - Microbiology 2019Quote: ... 2 mM MgSO4) and concentration of the sample using Vivaspin6 5 kDa MWCO concentrators (Sartorius).
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bioRxiv - Molecular Biology 2022Quote: ... 42 and 63 days were concentrated using 2 ml Vivaspin concentrator columns (Sartorius, Göttingen, Germany) by centrifuging at 1,000g until the final volume was 100 μl and subsequently centrifuged into the column cap at 4,000g for 2 min ...
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bioRxiv - Biochemistry 2024Quote: ... The supernatant from this second spin step was subsequently passed through 2 0.22µm filters (Sartorius) and made up to 500 ml with 50 mM ammonium acetate buffer at pH 5 before loading onto a 30 mL SP Sepharose column (Cytiva) ...
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bioRxiv - Cancer Biology 2021Quote: ... Fluorescent and phase contrast images are collected at intervals of 4 hours or shorter throughout the study in the Incucyte S2 Live Cell Analysis System (Essen/Sartorius) with temperature and environmental control ...
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bioRxiv - Microbiology 2019Quote: ... The eluted complex was then concentrated to 1 mL using a Vivaspin 4 Turbo concentrator (Cut-off 5 kDa; Sartorius). A final purification step was performed using size-exclusion chromatography by loading the proteins on a HiLoad 10/300 Superdex 200 column (GE Healthcare ...
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bioRxiv - Cell Biology 2022Quote: ... Lysates were spun at 15,000g for 15 min at 4°C and supernatants were then passed through a regenerated cellulose filter (4mm Ø, Sartorius). Samples were then dried under N2 flow at 40°C and resuspended in 125µl of ice-cold methanol/water 70:30 for subsequent analyses.
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... The resulting peptide solutions were centrifuged at 13,000 rpm for 15 min at 4 °C on tubes equipped with 2000 MW filtrate tubes (Sartorius, USA). Then ...
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bioRxiv - Biophysics 2021Quote: ... The fractions corresponding to hIP3R-3 were combined and concentrated to 4 mg/mL using a 100 kDa centrifugal filter (Sartorius). The concentrated sample was then centrifuged at 70,000 rpm using a S100AT rotor (Thermofisher) ...
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bioRxiv - Biophysics 2020Quote: ... MgCl2 and EGTA were removed by 4 times buffer exchange using a Vivaspin 500.5 molecular weight cut-off (Sartorius, Göttingen). Samples were loaded onto a SDS-PAGE gel to confirm phosphorylation.
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bioRxiv - Biochemistry 2019Quote: ... Fractions containing pure Fab16 were collected and concentrated to approximately 14 mg/ml using a VivaSpin 4 concentrator (10 kDa MWCO, Sartorius). Fab16 was crystallized by vapor diffusion at 4 °C by dispensing 200 nl of protein and 200 nl of crystallization buffer containing 1.5 M malic acid (pH 7.5) ...
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bioRxiv - Neuroscience 2020Quote: ... cells and media were collected and this mixture was centrifuged at 3700xg at 4°C to remove debris and then concentrated by ultrafiltration using Vivaflow 200 (Sartorius). The purified AAV particles were collected after running the viral concentrate through an iodixanol column (Opti-Prep density gradient medium ...
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bioRxiv - Immunology 2021Quote: ... The purified Spike and RBD recombinant proteins were then buffer exchanged into sterile PBS by centrifuging at 4000xg for 30 minutes at 4 °C in a 10,000 MWCO Vivaspin centrifugal concentrator (Sartorius, Germany) and stored at –80 °C ...
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bioRxiv - Microbiology 2023Quote: ... Protein was eluted using similar buffer with 300 mM imidazole and concentrated by centrifugation with 4 kDa (AlgF) or 30 kDa (AlgJ and AlgX) cutoff Vivaspin Turbo centrifugal concentrators (Sartorius). His6-tagged protein was further purified using a HiLoad 16/60 Superdex 200 prep-grade size exclusion column (GE Healthcare ...
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bioRxiv - Cell Biology 2023Quote: MuSCs were freshly isolated and seeded on 0.2% gelatin-coated 96 well plates at a density of 2,000 cells per well in growing conditions and incubated for 4 days in an IncuCyte SX5 Live-Cell Analysis System (Sartorius) at 37°C and 5% CO2 ...
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bioRxiv - Biochemistry 2023Quote: ... every 4 h (10x objective, 5 fields per well) and were analysed using the Incucyte Live-Cell Analysis System (Sartorius) and are represented as RCU x µm²/Well.
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bioRxiv - Immunology 2024Quote: ... The collagen suspension was mixed carefully with the cells in a ratio of 4:1 and 50 µl were added in a 96 well image lock plate (Sartorius) on ice ...
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bioRxiv - Bioengineering 2021Quote: Bioreactor cultivation was performed as duplicates in 2 L Biostat B-DCU bioreactors (Sartorius AG, Germany) with a working volume of 1.5 L ...
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bioRxiv - Plant Biology 2021Quote: ... Proteins eluting from the column were concentrated by ultrafiltration on Vivaspin 20 and 2 columns (Sartorius) with a 5 kDa molecular weight cut-off ...
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bioRxiv - Microbiology 2022Quote: ... Cells were disrupted by beat beating for 2 minutes using 0.4-0.6 mm glass beads (Sartorius). Beads were pelleted by centrifugation (21,000 × g ...
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bioRxiv - Microbiology 2023Quote: ... 2 L or 5 L scale (Biostat B-plus and B-DCU units, Sartorius Stedium Biotech) as previously described [PMID ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: EPO concentrated solution was stock in sterile and endotoxin-free 2 D bag (Sartorius Stedim, Germany) and all batches of EPO were pre-filled in SyriQ BioPure® 1mL syringe (Schott ...
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bioRxiv - Immunology 2023Quote: ... The GRAd32 S-2P vector was expanded in a 2 L Bioreactor (Biostat B DCU; Sartorius). The titer of virus contained in the bulk cell lysates was quantified by qPCR ...
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bioRxiv - Biochemistry 2023Quote: ... 2 mM DTT and concentrated to 200 μl with a 30-kDa cutoff concentrator (Sartorius, Germany). The protein concentration was determined via Bradford assay ...
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bioRxiv - Biochemistry 2020Quote: ... 4 °C) culture medium from two 90% confluent 15 cm dishes was filtered through a 1.2 μm filter (Sartorius Stedim Biotech) and applied to a HisTrap column (1 ml bed volume ...
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bioRxiv - Microbiology 2022Quote: ... The mixture was immediately vortexed for 4 seconds and rapidly filtered through cellulose acetate filters (0.45 μm, Sartorius Stedim Biotech GmbH). The biomass was washed on the filter with 10 mL of 60% methanol and stored at -70 °C until metabolite extraction.
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bioRxiv - Cancer Biology 2023Quote: ... The plates were spun down at 350g for 10 min at 4°C and placed in the Incucyte® Live-Cell Analysis System (Sartorius). The growth of the spheroids was monitored for the following 8 days and invasion was automatically quantified via the Incucyte® Spheroid module analysis tool ...
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bioRxiv - Cancer Biology 2022Quote: ... Medium containing DMSO or increasing concentration of test compound was added to each well and spheroid growth was monitored for 4 days post-treatment using the IncuCyte® Spheroid Analysis system (Sartorius). Images from 4 days post-treatment and day 0 were analyzed using the IncuCyte® Spheroid Analysis software module ...
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bioRxiv - Immunology 2022Quote: ... the supernatant was applied to 2 kDa centrifugal concentrators (Vivaspin 15 R Hydrosart filter device, Sartorius, Germany) and centrifuged for 1 h at 3000 x g according to the suppliers’ protocol ...
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bioRxiv - Microbiology 2022Quote: ... The samples were then concentrated from 40 mL to ∼2 mL using vivaspin 20 ultrafiltration units (Sartorius), and further concentrated to ∼100 µL using vivaspin 2 ultrafiltration units (Sartorius) ...
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bioRxiv - Microbiology 2022Quote: ... The samples were first concentrated from 2 L down to 40 mL using the vivaflow 200 (Sartorius). The samples were then concentrated from 40 mL to ∼2 mL using vivaspin 20 ultrafiltration units (Sartorius) ...
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bioRxiv - Cell Biology 2022Quote: ... the Spectrum polyP was desalted using a Vivacon 500 2 kDa cutoff spin filter (Sartorius, Bohemia, NY) at 7500 x g for 60 minutes at room temperature ...
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bioRxiv - Microbiology 2020Quote: ... The medium was filter sterilized using a 0.2 μm Sartopore 2 filter unit (Sartorius Stedim, Goettingen, Germany).
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bioRxiv - Immunology 2022Quote: ... followed by the removal of 2-MEA by buffer-exchanging to PBS using 100kDa Vivaspin6 columns (Sartorius) and storing overnight at 4 °C to allow for reoxidation of the disulfide bonds.
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bioRxiv - Synthetic Biology 2023Quote: ... The cell culture was inoculated at OD600 0.3 in the 2 L bioreactor (Biostat B, Sartorius, Germany) including the 1 L modified M9 minimal medium ...
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bioRxiv - Microbiology 2024Quote: ... A global association 1:2 bivalent analyte model using the Octet Data Analysis HT software v7 (Sartorius) was used to account for the dimeric HAstV-VA1 spike ...
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bioRxiv - Microbiology 2019Quote: ... viruses were concentrated by centrifugation at 4 °C using vivaspin 20 concentrators with a molecular weight cutoff of 100K (Sartorius, Göttingen, Germany). Thereafter ...
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bioRxiv - Cancer Biology 2020Quote: ... were homogenized in liquid nitrogen pre-cooled 4 ml PTFE-vials with one 8 mm stainless steel bead using a Micro-Dismembranator (Sartorius AG, Göttingen) at 2600 RPM for two minutes ...
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bioRxiv - Microbiology 2020Quote: ... liquid phage suspension was centrifuged at 4000 g for 10 min at 4°C and the supernatant was subsequently filter sterilised using a 0.45 μm pore sized membrane filter (Sartorius Minisart®, 16555). The filtrate phage lysate was stored at 4°C ...
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bioRxiv - Microbiology 2022Quote: The purified proteins were concentrated and exchanged to 10 mM sodium citrate buffer (pH 5.0) using Vivaspin® Turbo 4 ultrafiltration units with a 5000 MWCO PES membrane (Sartorius; Cat.# VS04T11). The purity and concentration of each PcaLOOL was assessed by SDS-PAGE and the Pierce™ BCA Protein Assay Kit ...
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bioRxiv - Neuroscience 2023Quote: ... The supernatant was concentrated by centrifugation for 1 hour at 3220 g at 4°C in a 5kDa Cutoff Vivaspin tube (Sartorius, Cat # VS2012). ACM was aliquoted into low protein binding tubes (∼100ul per aliquot) ...
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bioRxiv - Microbiology 2021Quote: ... Flow-through and wash fractions were pooled and concentrated using Vivaspin 15R concentrators (2 kDa MWCO HY, Sartorius).
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bioRxiv - Molecular Biology 2021Quote: ... diluted to a final Env trimer concentration of 2 mg/mL and filtered using 0.45 + 0.2 µm Sartobran 300 filter capsules (Sartorius). As final steps ...
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bioRxiv - Immunology 2020Quote: ... GFP fluorescent intensity was detected every 2 hours using the IncuCyte S3 Live-Cell analysis system (Sartorius, Germany). Total integrated GFP intensity per well was used as a quantitative measure of viable target cells ...
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bioRxiv - Biochemistry 2020Quote: ... Obtained fractions were analyzed by SDS PAGE and fractions containing proKLK14 were concentrated with Vivaspin® 2 (Sartorius) and further purified on Superdex s75 pg (GE Healthcare ...