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Citations for Sartorius :
151 - 200 of 217 citations for 7 Bromo 5 fluoro 3 methyl 1H indole since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... Cells were pelleted by centrifugation (16,000g, 5 min) and the supernatant passed through a 0.22 μm nitrocellulose filter (Sartorius). Proteins were precipitated with 10% trichloroacetic acid (TCA ...
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bioRxiv - Cell Biology 2022Quote: ... cells were placed in the Incucyte SX5 and maintained at 37°C and 5% CO2 (Sartorius, Göttingen, Germany) for detection and automated analysis of cell growth/proliferation ...
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bioRxiv - Microbiology 2024Quote: ... samples were clarified using a syringe with a filter cascade (5 µm, 1.2 µm, 0.8 µm, 0.45 µM, Sartorius AG). The cell pellets were not further analysed ...
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bioRxiv - Bioengineering 2024Quote: ... After 24 hrs supernatant was collected and concentrated by the Vivaspin-6 5 kDa MWCO filters (Sartorius VS06S1). Cells were lysed in 20 mM Tris-HCl 7.4 pH at 4 °C for 1 hr ...
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bioRxiv - Bioengineering 2023Quote: ... was washed three times in DI water and concentrated to a final volume of 1 mL using 3 kDa MWCO ultrafiltration tubes (Sartorius Stedim Biotech, Germany). The tubes were spun at 4000 rpm for 40 minutes ...
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bioRxiv - Biochemistry 2022Quote: ... and buffer was exchanged four times with 5 mL of 10 mM potassium phosphate buffer pH 7.4 using a Vivaspin 6 Centrifugal Concentrator (Sartorius) at 2,000 rcf and 4°C for 20 min ...
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bioRxiv - Microbiology 2022Quote: ... and BA.2+R493Q RBDs were diluted to 5 μg/mL and immobilized onto pre-hydrated streptavidin biosensors (Sartorius) to a level of 1 nm total shift ...
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bioRxiv - Cell Biology 2019Quote: Live-cell imaging was performed at 37°C under humidified 5% CO2 atmosphere using the IncuCyte Zoom system (Sartorius). Phase and fluorescence images were also acquired using Leica DiM8 and Zeiss LSM710 microscopes ...
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bioRxiv - Molecular Biology 2021Quote: ... The fractions containing the protein-RNA complex were concentrated to 400-700 uM with Vivaspin 5-mL centrifugal devices with 10,000 MWCO (Sartorius). Before the measurements a 10% molar excess of RNA was added to saturate the protein as well as 10% v/v D2O ...
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bioRxiv - Microbiology 2022Quote: ... The supernatant was sterile-filtered using 0.2µm pore size syringe filters with low protein binding (Sartorius, ref.:16534-5). The obtained FW was stored at -80°C until further analysis.
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bioRxiv - Microbiology 2022Quote: ... 5 mM MgCl2) and concentrated to ~1 mg/mL by Vivaspin 20 centrifugal filter unit (MWCO 10 kDa) (Sartorius). Sfp synthase and CoA-conjugated dyes were prepared as previously described54 and used to perform the overnight Fab labeling reaction on ice ...
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bioRxiv - Biochemistry 2023Quote: ... 5 mM MgCl2 by repeated ultracentrifugation using a Vivaspin 500 filter device with a cutoff of 30 kDa (Sartorius) at 4 °C and afterwards concentrated ...
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bioRxiv - Biochemistry 2023Quote: ... Samples were incubated at room temperature during one hour and labeled proteins were separated from Ir2 and DMSO vehicle by 5 cycles of diafiltration (12,000 g, 4°C, molecular cutoff 10kDa, Sartorius) adding 5 volumes of labeling buffer before each cycle ...
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bioRxiv - Biophysics 2023Quote: ... using an acquisition rate of 5 Hz averaged by 20 using an Octet© R8 BLI System (FortéBio/Sartorius), with assays designed and setup using Blitz Pro 1.3 Software ...
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bioRxiv - Biochemistry 2023Quote: ... The resulting protein was concentrated to ≈4 mg/mL using a 5 kDa centrifugal concentrator (Vivaspin; Sartorius, Gottingen, Germany). For drop-deposition Raman spectroscopy ...
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bioRxiv - Cell Biology 2024Quote: ... The plate was incubated at 37°C and 5% CO2 and imaged using an Incucyte Live-Cell imager (Sartorius). The red channel used an acquisition time of 400 milliseconds for all replicates and samples ...
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bioRxiv - Cell Biology 2024Quote: ... pollen contained in 5 ml suspension was vacuum-filtrated onto a round polyamide membrane filter (diameter 5 cm, pore size 0.45 µm; Sartorius) and transferred onto 3.5 ml solid (0.25 % phytagel [Sigma-Aldrich] ...
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bioRxiv - Microbiology 2019Quote: ... The eluted complex was then concentrated to 1 mL using a Vivaspin 4 Turbo concentrator (Cut-off 5 kDa; Sartorius). A final purification step was performed using size-exclusion chromatography by loading the proteins on a HiLoad 10/300 Superdex 200 column (GE Healthcare ...
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bioRxiv - Microbiology 2019Quote: ... cells were stained with SYBR Green I (Marie et al., 1997) and large suspended particles were removed by filtration through 5 µm pore-size filters (Sartorius) to avoid clogging of the flow cytometer ...
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bioRxiv - Cell Biology 2021Quote: ... Brightfield images were taken every 2 hours for 5 days and the percentage confluency calculated using an Incucyte Live-Cell analysis system (Sartorius).
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bioRxiv - Biophysics 2022Quote: BLI analyses were performed at 25°C at an acquisition rate of 5 Hz averaged by 20 using an Octet© BLItz System (FortéBio/Sartorius) with assays were designed and setup using Blitz Pro 1.3 Software ...
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bioRxiv - Biochemistry 2020Quote: ... 5% ethylene glycol), followed by buffer exchange to (30 mM Tris/HCl, pH 8.0) with Vivaspin centrifugal concentrator (Sartorius, Germany). The final phosphoprotein concentrations were adjusted to 1.5 mg/ml (80 μM) ...
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bioRxiv - Biochemistry 2021Quote: The dialysed proteins were concentrated to a <5 mL volume using a 30 kDa MWCO Vivaspin 20 centrifugal concentrator (Sartorius) at 5000 g ...
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bioRxiv - Cell Biology 2022Quote: ... individual frames of cells growing under normal culture conditions were acquired every 5 minutes for 24 hours using an IncuCyte ZOOM live-cell analysis system (Sartorius) equipped with a 20 x objective.
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bioRxiv - Bioengineering 2020Quote: ... The fractions containing MTG variants were concentrated to a volume of approximately 5 mL using ultrafiltration membrane of 10 kDa MWCO (Vivaspin Turbo 15, Sartorius). Subsequently ...
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bioRxiv - Biophysics 2020Quote: ... Peak fractions containing purified Cx46/50 were pooled and concentrated to 5–6 mg mL-1 with a centrifugal device (Vivaspin 6; 50-kDa cut-off filter; Sartorius). Protein concentration was determined by UV absorbance at 280 nm ...
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bioRxiv - Immunology 2020Quote: ... The EV pellet was resuspended in 2 ml PBS and stored at −80 prior to further concentration using a 5 kDa MW cut-off vivaspin (Sartorius). The protein content of EVs was quantified using Qubit (Invitrogen ...
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bioRxiv - Biochemistry 2021Quote: ... Biotinylated-NIH-CoVnb-112 was diluted into assay buffer at 5 µg/mL and immobilized onto streptavidin coated biosensors (#18-5019, Sartorius) to a minimum response value of 1 nm on the Octet Red96 System (Sartorius) ...
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bioRxiv - Immunology 2022Quote: ... Biotinylated NPNA peptides were diluted to 5 μg/mL in kinetics buffer (KB) and immobilized onto streptavidin BLI biosensors (Sartorius); His-tagged rsCSP was diluted to ~1 μg/mL in KB and loaded onto Ni-NTA biosensors ...
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bioRxiv - Cancer Biology 2022Quote: ... 5 x 104 cells were seeded in each well of an Incucyte® ImageLock 96-well plate (Sartorius, Catalog #: 4379) in 100 μL/well of either complete RPMI or DMEM ...
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bioRxiv - Biochemistry 2022Quote: ... Fractions containing biotinylated primer bound to monovalent streptavidin were subsequently annealed to a 5’ biotinylated DNA template before binding to streptavidin biosensors (Sartorius). 200 nM of DNA substrates were added in the loading step ...
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bioRxiv - Microbiology 2022Quote: ... and 0.5% nonfat dry milk) to 100 nM and immobilized for 60 s to anti-human IgG Fc capture biosensors (Sartorius). Sensors were transferred to assay buffer for 30 s to set the baseline ...
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bioRxiv - Neuroscience 2022Quote: ... The pooled fractions of the NOTCH3 EGF1-5 WT and R133C were concentrated to 1 mg/mL in a concentration column (Vivaspin 6 10 KDa MWCO, Sartorius) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... fractions containing the protein of interest were pooled together and concentrated using Vivaspin 20 centrifugal concentrators with a 5 kDa cut-off (Sartorius). The concentration of BRD4 BD1 was determined spectrophotometrically at 280 nm using the calculated molar extinction coefficient ...
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bioRxiv - Cancer Biology 2023Quote: ... fractions containing FKBP12 F36V were pooled together and concentrated using Vivaspin 20 centrifugal concentrators with a 5 kDa cut-off (Sartorius). The concentration of FKBP12 F36V was determined spectrophotometrically at 280 nm using the calculated molar extinction coefficient ...
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bioRxiv - Immunology 2023Quote: ... Mouse antibodies (7.5 μg/mL anti-OVA and 5 μg/mL anti-RBD) were captured on pre-conditioned anti-mouse IgG Fc-capture biosensors (AMC, Sartorius) for 600 s ...
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bioRxiv - Biochemistry 2023Quote: ... every 4 h (10x objective, 5 fields per well) and were analysed using the Incucyte Live-Cell Analysis System (Sartorius) and are represented as RCU x µm²/Well.
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bioRxiv - Immunology 2023Quote: ... The cocultures were incubated for 48 hours in a humidified incubator (37 °C, 5% CO2) equipped with an Incucyte (Sartorius) for continuous monitoring of cell proliferation and target cell killing ...
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bioRxiv - Developmental Biology 2024Quote: ... for a total of 15-20 minutes with trituration every 5 minutes using 200 μl low bind tips (Sartorius, 790101F). Papain was inactivated using an Ovomucoid inhibitor (Worthington Papain Dissociation Kit ...
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bioRxiv - Immunology 2024Quote: ... Biotinylated OspA (5 µg/mL) in PBS containing 2% w/v BSA was captured onto streptavidin biosensors (#18-5019, Sartorius) for 5 min ...
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bioRxiv - Biochemistry 2020Quote: ... SARS-CoV-2 S1 RBD was buffer-exchanged (prior to spectral analysis) using a 5 kDa Vivaspin centrifugal filter (Sartorius, Germany) at 12,000 g ...
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bioRxiv - Biochemistry 2020Quote: ... SARS-CoV-2 S1 RBD was buffer-exchanged (prior to spectral analysis) using a 5 kDa Vivaspin centrifugal filter (Sartorius, Germany) at 12,000 g ...
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bioRxiv - Plant Biology 2021Quote: ... the pH of the hydroponic culture media of the 5 treatments was measured using a pH meter (PB-10, Sartorius, Germany).
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bioRxiv - Plant Biology 2020Quote: ... Material for analyses was obtained from seeds pre-cooled for 15 min in 5-mL Teflon capsules (Sartorius GmbH, Göttingen, Germany), containing a 10 mm-diameter agate bead ...
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bioRxiv - Biochemistry 2022Quote: ... A template with a biotin-TEG at the 5’-end was annealed to the primers (Table 2) and immobilized on a streptavidin-coated biosensor (SAX, Sartorius AG). Primers were added at two-fold molar excess over the template ...
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bioRxiv - Biochemistry 2020Quote: ... SARS-CoV-2 S1 RBD was buffer-exchanged (prior to spectral analysis) using a 5 kDa Vivaspin centrifugal filter (Sartorius, Germany) at 12,000 g ...
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bioRxiv - Biochemistry 2020Quote: ... SARS-CoV-2 S1 RBD was buffer-exchanged (prior to spectral analysis) using a 5 kDa Vivaspin centrifugal filter (Sartorius, Germany) at 12,000 g ...
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bioRxiv - Microbiology 2021Quote: ... using a centrifugation tube with an insert containing a molecular weight cut-off filter (5 kDa, Sartorius Stedim Biotech, Goettingen, Germany). Each HMW fraction was purified by flushing with 20 ml of water ...
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bioRxiv - Microbiology 2021Quote: ... the fixed cells were washed three times with PBS and the cell suspension was filtered with a 5 μm pore size filter (Sartorius, 17594) to remove aggregates ...
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bioRxiv - Bioengineering 2023Quote: Sylgard-184™ silicone elastomer kit PDMS pre-polymer was well mixed (5 mins) with curing agent at a 10:1 w/w ratio using a digital balance (Sartorius BP610) prior to vacuum desiccation and casting at various temperatures (60-90°C).