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Citations for Sartorius :
301 - 350 of 389 citations for 7 11 Dimethyldodeca 4 6 10 trien 3 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... Buffer exchange and protein sample concentration were performed using 10 kDa MWCO Vivaspin Turbo centricons (Sartorius, Germany). After IMAC ...
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bioRxiv - Neuroscience 2020Quote: ... after which conditioned media was collected and concentrated 50-fold using 10 kDa cutoff concentrators (Sartorius #14558502). Protein concentration was measured using the Bradford assay ...
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bioRxiv - Biochemistry 2023Quote: ... The fractions containing LPMO were pooled and concentrated using ultrafiltration spin-tubes (10 kDa cut-off, Sartorius). The protein-containing fractions were analyzed by SDS-PAGE.
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bioRxiv - Biochemistry 2023Quote: ... S120A-FsC or L182A-FsC were pooled and concentrated using centrifugal concentrators (10 kDa cut-off, Sartorius). The protein concentration was calculated by measuring A280 using Nanodrop and the theoretical extinction coefficient (ε = 14690 M-1 cm-1) ...
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bioRxiv - Microbiology 2021Quote: ... The samples were weight with scales from KERN ALS 220-4 (KERN & SOHN GmbH, Balingen-Frommern, Germany) and Sartorius Cubis®-series (Sartorius AG, Göttingen, Germany). During the extraction process ...
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bioRxiv - Physiology 2023Quote: ... Bundles were lyophilized in a freeze-dryer (Labconco, Kansas City, MO, USA) for >4 h and weighed on a microbalance (Sartorius Cubis Microbalance, Gottingen, Germany). The rate of mH2O2 emission was calculated using a standard curve under the same assay conditions and then normalized to fibre bundle dry weight.
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bioRxiv - Plant Biology 2020Quote: ... The eluates were concentrated on Vivaspin 500 10 kDa molecular weight cut-off (MWCO) filter/concentrator columns (Sartorius) and the proteins stored at −20°C (1 to 5 mg ml-1 ...
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bioRxiv - Molecular Biology 2021Quote: ... to 0.1 M Glycine / 10 mM Tris / pH 7.5 using a Sartocon Slice ECO (30 kDa MWCO, 0.14 m²) PES membrane (Sartorius). Trimer-containing fractions were pooled and passed over a pre-equilibrated MAbSelect SuRe Protein A (ProA ...
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bioRxiv - Molecular Biology 2021Quote: ... injection grade (≥ 10 volume changes) using a Sartocon Slice ECO (30 kDa MWCO, 0.14 m²) PES membrane (Sartorius). Buffer exchanged DS preparations were further adjusted to a concentration of ∼1 mg x mL-1 in 20 mM Tris / 150 mM NaCl / pH 7.5 ...
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bioRxiv - Microbiology 2022Quote: ... Supernatants were centrifuged at 2000 rpm for 10 minutes and passed through a 0.45 µm syringe filter (Sartorius) to remove debris prior to concentration by ultracentrifugation at 25000 rpm for 2 hours at 4°C ...
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bioRxiv - Cell Biology 2022Quote: ... His-PKR was further concentrated to at least 10 mg/ml using Vivaspin centrifugal concentrator 30 K (Sartorius), snap-frozen in liquid nitrogen and stored at −80 °C.
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... plates were scanned with a 10× objective using the Incucyte ZOOM imaging system (Sartorius, Ann Arbor, MI, USA). Normalized GFP expression (GCU ...
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bioRxiv - Immunology 2022Quote: ... The supernatant was harvested after removing cells by centrifugation at 2,000 g for 10 min and filtered on a Sartobran P sterile capsule of 0.45 μm (Sartorius). The buffer was then exchanged to buffer A (150 mM NaCl ...
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bioRxiv - Microbiology 2023Quote: ... Purified protein fractions were dialyzed against 1X PBS (pH 7.4) using the Vivaspin 10 kDa centrifuge system (Sartorius). Protein quantification was performed in a spectrophotometer at 280 nm and the assays were performed with the enzyme at a concentration of 5.88 μM.
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bioRxiv - Biochemistry 2023Quote: ... Fractions that contained BLMcore and no detectable nuclease activity were concentrated in Vivaspin-20 10 kDa concentrator (Sartorius) to 2 mL and loaded onto the HiPrep 16/60 Sephacryl S-300 HR column (Cytiva ...
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bioRxiv - Cell Biology 2023Quote: ... The cell-free supernatant (CFS) was concentrated to 10 mL with a 100 kDa ultrafiltration cassette (Sartorius, VF05H4) attached to a peristaltic pump ...
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bioRxiv - Microbiology 2023Quote: ... Culture supernatants were obtained by centrifugation (8,000 g, 10 min) and filtration (0.22 μm filters; Sartorius, Göttingen, Germany). Stx production was tested using Vero cell cytotoxicity assay (supplement) ...
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bioRxiv - Immunology 2023Quote: ... and 37.7H IgG were diluted to concentrations of 10 µg/mL and loaded onto Protein A sensors (Sartorius) to a binding signal of 1.0 nM ...
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bioRxiv - Immunology 2020Quote: ... Frames were captured over a period of 24 hrs at 1 hour intervals from 4 separate 1.75 x 1.29 mm2 regions per well with a 10× objective using IncuCyte S3 live-cell analysis system (Sartorius). Values from all four regions of each well were pooled and averaged across all three replicates ...
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bioRxiv - Immunology 2020Quote: ... Frames were captured over a period of 24hrs at 1-hour intervals from 4 separate 1.75 x 1.29 mm2 regions per well with a 10× objective using IncuCyte S3 live-cell analysis system (Sartorius). Values from all four regions of each well were pooled and averaged across all three replicates ...
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bioRxiv - Biochemistry 2021Quote: ... performed by ultrafiltration through a 10 kDa cut-off polyether sulfone membrane (Vivacell 250, Sartorius Stedim Biotech GmbH, Germany). The buffered supernatant was loaded on a DEAE-20 mL HiPrep FF 16/10 anion exchange chromatography column (GE Healthcare) ...
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bioRxiv - Cancer Biology 2021Quote: ... the 10,000xg supernatant was concentrated down to 1.0 ml with a 10 kDa molecular weight cut-off Vivaflow 50 R tangential flow (TFF) device (Sartorius) and a 10 kDa Amicon Ultra-15 centrifugal filter unit (Merck Millipore ...
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bioRxiv - Microbiology 2021Quote: ... the buffer was exchanged to 50 mM Tris-HCl (pH 7.9) without sucrose using 10 kDa mass cutoff centrifugal filters (Sartorius Vivaspin 500 ...
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bioRxiv - Immunology 2022Quote: ... cell culture supernatants where cleared by centrifugation at 2000 x g for 10 min followed by filtration with a 1.2 μm Minisart NML (Sartorius Stedim Biotech GmbH ...
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bioRxiv - Bioengineering 2022Quote: ... the appropriate volume of stock solution was centrifuged at 3000g at 10 °C in Vivaspin® 20 concentrators (Sartorius Stedim Biotech GmbH ...
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bioRxiv - Microbiology 2022Quote: ... 5 mM MgCl2) and concentrated to ~1 mg/mL by Vivaspin 20 centrifugal filter unit (MWCO 10 kDa) (Sartorius). Sfp synthase and CoA-conjugated dyes were prepared as previously described54 and used to perform the overnight Fab labeling reaction on ice ...
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bioRxiv - Microbiology 2022Quote: ... the combined clarified fluid was directly concentrated to 10-50 fold by Vivaspin 20 Centrifugal Concentrators (Sartorius, Gottingen, Germany) with PES membrane of 100k MW cut off ...
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bioRxiv - Immunology 2022Quote: ... Cells were washed again with PBS before DMEM + 10% FBS was added with 250nM Incucyte® Cytotox Green (Sartorius) +/- 1 μM Imatinib ...
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bioRxiv - Immunology 2023Quote: ... Anti-HA antibodies were diluted in kinetics buffer (PBS with 0.5% serum bovine albumin and 0.01% Tween) to a final concentration of 10 μg/mL before loading onto protein A biosensors (Sartorius) for 200 s ...
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bioRxiv - Microbiology 2023Quote: ... This monodisperse peak was pooled and concentrated to 20 mg/ml with a 10 kDa MWCO ultrafiltration device (Sartorius).
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bioRxiv - Bioengineering 2023Quote: ... Buffer of AFC eluate was then exchanged by AEX equilibration buffer using 10 kDa NMWCO Vivaspin (Sartorius Stedim, Germany) column then applied in AEX resin ...
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bioRxiv - Immunology 2023Quote: ... Anti-HA antibodies were diluted in kinetics buffer (PBS with 0.5% serum bovine albumin and 0.01% Tween) to a final concentration of 10 μg/mL before loading onto protein A biosensors (Sartorius) for 200 s ...
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bioRxiv - Cancer Biology 2023Quote: ... SK-N-MC and ES5838 (4 x 104) cells per well were plated in a 24-well plate and placed in an IncucyteS3 incubator (Sartorius, Essen BioScience, Inc. Ann Arbor, MI). Nine images per well were taken every 6 hrs for seven days ...
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bioRxiv - Biochemistry 2020Quote: ... glycerol was added and the protein concentrated to 20 – 30 mg/mL in a 10 kDa MWCO spin concentrator (Vivaspin, Sartorius).
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bioRxiv - Molecular Biology 2020Quote: ... The resulting CM was then concentrated to approximately 10 ml using the Vivaflow 50R tangential flow device (Sartorius, Göttingen, Germany) with a 100 kDa cut-off membrane ...
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bioRxiv - Microbiology 2020Quote: ... Protein samples were quantified by BCA assay (Pierce) and transferred to a 10 kDa MWCO spin filter (Vivaspin 500; Sartorius) before centrifugation at 12,000 x g to collect denatured and reduced proteins atop the filter membrane ...
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bioRxiv - Biochemistry 2022Quote: ... Fractions containing the proteins were identified by SDS-PAGE and concentrated using a Vivaspin Turbo 15 centrifuge filter with a 10 kDa molecular weight cutoff (Sartorius). Glycerol was added to a final concentration of 25% ...
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bioRxiv - Plant Biology 2022Quote: ... The eluate (3x 500 μl) was concentrated by ultrafiltration (Vivaspin concentrators, 10 kDa molecular weight cut-off; Sartorius; Göttingen, Germany) and dialyzed against 50 mM Na2HPO4/NaH2PO4 pH 6.0 ...
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bioRxiv - Bioengineering 2020Quote: ... The fractions containing MTG variants were concentrated to a volume of approximately 5 mL using ultrafiltration membrane of 10 kDa MWCO (Vivaspin Turbo 15, Sartorius). Subsequently ...
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bioRxiv - Cancer Biology 2022Quote: ... Basolateral compartments were filled with culture medium containing 10% FBS and plates were placed in an Incucyte S3 Imaging System (Sartorius). Images of the apical and basolateral sides of the porous membrane were captured every 4 hours over 3 days ...
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bioRxiv - Biochemistry 2020Quote: ... The protein was expressed and purified as previously described.26 The purified protein was concentrated to 10-20 mg/mL using a Vivaspin15R (Sartorius), and purity was estimated to be at least 95% by SDS-PAGE.
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bioRxiv - Biochemistry 2021Quote: ... The protein solution was concentrated to 10 – 20 mg/mL using a Centrisart I MWCO 100 kDa concentrator (Sartorius AG).
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bioRxiv - Molecular Biology 2021Quote: ... Cellular debris were spun down for 10 min at 9500x g and the concentrate was filtered with a glass fiber prefilter (Minis-art, Sartorius). Protein was purified by Nickel-nitrilotriacetic acid (Ni-NTA ...
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bioRxiv - Immunology 2022Quote: ... Bench scale production was done in 10 liters bioreactor glass connected to Biostat BDCU controlling system (Sartorius-Stedim, Bohemia, NY). The cells grew in SFX-insect media (Hyclone ...
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bioRxiv - Microbiology 2022Quote: ... The fractions containing the sample of interest were pooled and concentrated using 10 kDa (MtPAPP) and 30 kDa cut-off centrifugal concentrator (Sartorius). The yield of this purification was ...
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bioRxiv - Microbiology 2023Quote: ... were deposited on a 35 mm x 10 mm polystyrene petri dish (Falcon, Cat. No. 351008) and placed on a microbalance (Sartorius Cubis I ...
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bioRxiv - Genetics 2023Quote: ... 500 cells were seeded in duplicates in 96-well plates and treated with 1 µg/ml dox for 9-10 days and analyzed with Incucyte (Sartorius). Untreated cells served as reference.
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bioRxiv - Microbiology 2023Quote: ... buffer was exchanged to PBS (same volume of the initial serum) using 10 kDa MWCO Vivaspin6 filters (Sartorius, Gӧttingen, Germany) and the resulting preparations were used to measure BG505/T332N neutralization as described above.
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bioRxiv - Biophysics 2023Quote: ... The flow-through volume containing the untagged protein was concentrated to 10 mg/mL using Vivacon500 filters (50 kDa, Sartorius) for further purification using a Superdex 200 Increase 5/150 GL column equilibrated in 50 mM Tris-HCl pH 7.0 ...
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bioRxiv - Plant Biology 2024Quote: ... FLVB and FLVA/B were analyzed by SDS-page and then concentrated by ultra filtration (Sartorius, Vivaspin, 10 kDa cutoff). Analysis of different purification steps and quality of protein purifications was also assessed by Immunoblot analysis with custom-made α-FLVA ...