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Citations for Sartorius :
401 - 450 of 499 citations for 6H Dibenzo a g quinolizinium 5 8 13 13a tetrahydro 2 9 dihydroxy 3 10 dimethoxy 7 methyl 7S 13aS since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... plates were scanned with a 10× objective using the Incucyte ZOOM imaging system (Sartorius, Ann Arbor, MI, USA). Normalized GFP expression (GCU ...
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bioRxiv - Microbiology 2023Quote: ... Purified protein fractions were dialyzed against 1X PBS (pH 7.4) using the Vivaspin 10 kDa centrifuge system (Sartorius). Protein quantification was performed in a spectrophotometer at 280 nm and the assays were performed with the enzyme at a concentration of 5.88 μM.
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bioRxiv - Biochemistry 2023Quote: ... Fractions that contained BLMcore and no detectable nuclease activity were concentrated in Vivaspin-20 10 kDa concentrator (Sartorius) to 2 mL and loaded onto the HiPrep 16/60 Sephacryl S-300 HR column (Cytiva ...
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bioRxiv - Cell Biology 2023Quote: ... The cell-free supernatant (CFS) was concentrated to 10 mL with a 100 kDa ultrafiltration cassette (Sartorius, VF05H4) attached to a peristaltic pump ...
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bioRxiv - Molecular Biology 2023Quote: ... pH 6.0) using a centrifugal concentrator with a molecular weight cutoff of 10 kDa (Vivaspin 6, VS0602, Sartorius). The presence of the fucosidase (62 kDa ...
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bioRxiv - Immunology 2023Quote: ... and 37.7H IgG were diluted to concentrations of 10 µg/mL and loaded onto Protein A sensors (Sartorius) to a binding signal of 1.0 nM ...
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bioRxiv - Immunology 2022Quote: ... 200 nM of randomly biotinylated SARS-CoV-2 S or RBD protein antigen was captured using SA biosensors (18-5019, Sartorius). The biosensor was loaded with antigen for 5 min and then moved into the saturating mAbs at a concentration of 100 µg/mL for 10 min ...
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bioRxiv - Cancer Biology 2019Quote: ... The cell monolayer was scraped and migration was monitored via time-lapse image acquisition every 2 hours via the IncuCyte ZOOM imaging platform (Sartorius). Migration rates were calculated as relative wound density over time for wildtype and mutant cell lines ...
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DNA uptake by cell wall-deficient bacteria reveals a putative ancient macromolecule uptake mechanismbioRxiv - Microbiology 2022Quote: ... Fragmented gDNA was obtained by beat-beating the intact gDNA for 12 minutes using 2 mm diameter glass beads in a Mikro-Dismembrator U (Sartorius) at 2000 rpm ...
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bioRxiv - Systems Biology 2020Quote: 2 mL of collected spent media was loaded in Vivaspin 6™ Centrifugal Concentrator 10,000 MWCO PES (Sartorius AG, Germany) and centrifuged at 4000 RPM for 13 minutes to concentrate samples ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Images and confluence data were acquired every 2 h for ~200 h using the IncuCyte Live Cell Analysis Imaging System (Sartorius). The Area Under Curve (AUC ...
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bioRxiv - Biochemistry 2022Quote: The specificity of the binding consisted in the immobilization of 2 μg/mL of purified VHHs on Anti-His coated sensors (His1K, Sartorius) via their His6 tag ...
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bioRxiv - Immunology 2023Quote: ... pseudotyped SARS-CoV-2 virus particles were harvested and filtered over a 0.45 µm nitrocellulose membrane (Sartorius Stedim, Gottingen, Germany). SARS-CoV-2 pseudovirus productions were quantified by RETRO-TEK HIV-1 p24 ELISA according to manufacturer instructions (ZeptoMetrix Corporation).
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bioRxiv - Cell Biology 2023Quote: Isolated ECs were seeded onto 96 well-plates pre-coated with fibronectin in EGM-2 and the proliferation assay was performed for 60 hours in the Incucyte proliferation assay according to (21) (Sartorius). The analysis of the cell proliferation was performed by the Incucyte Basic Analyzer software.
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bioRxiv - Bioengineering 2022Quote: ... Four images were recorded per well every 2 h and analyzed using IncuCyte cell by cell analysis software module (Sartorius). The killing potency of the CAR-T cells was assessed by comparing the percentage of Annexin V positive target cells over time relative to the total number of target cells.
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bioRxiv - Bioengineering 2023Quote: ... This experiment was carried out in a commercial stirred tank bioreactor (STBR) of 2 l total volume (Sartorius AG, Germany) and a working volume of 1 l was used ...
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bioRxiv - Molecular Biology 2023Quote: ... The monodisperse peak of the complex was collected and concentrated with a 50 kDa cut-off concentrator (Vivaspin 2, Sartorius) and subsequently used for crystallization in LCP.
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bioRxiv - Cell Biology 2023Quote: ... The remaining vesicle pellets were resuspended in 2 mL sterile 1× PBS and sterile filtered with 0.20 µm syringe filters (Carl Roth, Sartorius VS0271).
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bioRxiv - Biochemistry 2020Quote: ... and the filtrate concentrated using a ultrafiltration apparatus with a 5 kDa molecular mass cut-off (Vivaflow® 200 cassette, Sartorius Stedim Biotech). The concentrate was separated by reversed phase (RP-HPLC ...
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bioRxiv - Biochemistry 2020Quote: ... we concentrated the proteins from the supernate by membrane centrifugation using 5 K molecular weight cutoff Vivaspin units of 6 mL (Sartorius Stedim, Goettingen, Germany). The retentate (~300 μL ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Samples weighing 5-15mg were dispensed in standard aluminum pans (DSC consumables incorporated, Minnesota, USA) using a microbalance (Sartorius 3.6P microbalance, Göttingen, Germany) and sealed using standard aluminum lids ...
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bioRxiv - Genomics 2024Quote: The human T lymphoblast Sup T1 cell line was cultured at 37°C under a 95% air and 5% CO2 atmosphere in Roswell Park Memorial Institute medium (RPMI 1640 medium; Sartorius #01-100-1A) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Immunology 2020Quote: ... Frames were captured over a period of 24 hrs at 1 hour intervals from 4 separate 1.75 x 1.29 mm2 regions per well with a 10× objective using IncuCyte S3 live-cell analysis system (Sartorius). Values from all four regions of each well were pooled and averaged across all three replicates ...
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bioRxiv - Immunology 2020Quote: ... Frames were captured over a period of 24hrs at 1-hour intervals from 4 separate 1.75 x 1.29 mm2 regions per well with a 10× objective using IncuCyte S3 live-cell analysis system (Sartorius). Values from all four regions of each well were pooled and averaged across all three replicates ...
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bioRxiv - Biochemistry 2021Quote: ... performed by ultrafiltration through a 10 kDa cut-off polyether sulfone membrane (Vivacell 250, Sartorius Stedim Biotech GmbH, Germany). The buffered supernatant was loaded on a DEAE-20 mL HiPrep FF 16/10 anion exchange chromatography column (GE Healthcare) ...
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bioRxiv - Cancer Biology 2021Quote: ... the 10,000xg supernatant was concentrated down to 1.0 ml with a 10 kDa molecular weight cut-off Vivaflow 50 R tangential flow (TFF) device (Sartorius) and a 10 kDa Amicon Ultra-15 centrifugal filter unit (Merck Millipore ...
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bioRxiv - Microbiology 2021Quote: ... the buffer was exchanged to 50 mM Tris-HCl (pH 7.9) without sucrose using 10 kDa mass cutoff centrifugal filters (Sartorius Vivaspin 500 ...
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bioRxiv - Bioengineering 2022Quote: ... the appropriate volume of stock solution was centrifuged at 3000g at 10 °C in Vivaspin® 20 concentrators (Sartorius Stedim Biotech GmbH ...
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bioRxiv - Microbiology 2022Quote: ... the combined clarified fluid was directly concentrated to 10-50 fold by Vivaspin 20 Centrifugal Concentrators (Sartorius, Gottingen, Germany) with PES membrane of 100k MW cut off ...
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bioRxiv - Immunology 2022Quote: ... Cells were washed again with PBS before DMEM + 10% FBS was added with 250nM Incucyte® Cytotox Green (Sartorius) +/- 1 μM Imatinib ...
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bioRxiv - Microbiology 2023Quote: ... This monodisperse peak was pooled and concentrated to 20 mg/ml with a 10 kDa MWCO ultrafiltration device (Sartorius).
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bioRxiv - Immunology 2023Quote: ... Anti-HA antibodies were diluted in kinetics buffer (PBS with 0.5% serum bovine albumin and 0.01% Tween) to a final concentration of 10 μg/mL before loading onto protein A biosensors (Sartorius) for 200 s ...
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bioRxiv - Bioengineering 2023Quote: ... Buffer of AFC eluate was then exchanged by AEX equilibration buffer using 10 kDa NMWCO Vivaspin (Sartorius Stedim, Germany) column then applied in AEX resin ...
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bioRxiv - Biophysics 2023Quote: ... The eluted protein was then concentrated to the approximate concentration of 10 mg/ml using a Vivaspin 6 3,000 MWCO PES (Sartorius) membrane ultrafiltration tube.
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bioRxiv - Immunology 2023Quote: ... Anti-HA antibodies were diluted in kinetics buffer (PBS with 0.5% serum bovine albumin and 0.01% Tween) to a final concentration of 10 μg/mL before loading onto protein A biosensors (Sartorius) for 200 s ...
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bioRxiv - Microbiology 2019Quote: ... cells were fixed in 2% paraformaldehyde and GFP positive cells were quantified by flow cytometry (Intellicyt iQue Screener Plus, Sartorius AG). Dose-response neutralization curves were analyzed by non-linear regression with a variable slope (GraphPadPrism v8 ...
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bioRxiv - Microbiology 2020Quote: ... 1% (w/v) polyvinylpyrrolidone and 2% (w/v) sodium dodecyl sulfate after which 200 mg glass beads (0.11 mm Sartorius, Gottingen, Germany) were added and the cells were lysed for 5 min at 2000 rpm in a FastPrep VR-96 instrument (MP Biomedicals ...
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bioRxiv - Immunology 2020Quote: ... and S309 for binding to soluble SARS-CoV-2 S trimer was assessed using the ForteBio Octet HTX (Sartorius Bioanalytical Instruments). All reagents were diluted to 100 nM in PBSF ...
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The Kinase Chemogenomic Set (KCGS): An open science resource for kinase vulnerability identificationbioRxiv - Cell Biology 2019Quote: ... 50 µl media with 2 x compound concentration was added and plates were subsequently placed and monitored in an IncuCyte® (Sartorius) instrument ...
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bioRxiv - Microbiology 2020Quote: Batch and fed-batch bioreactor experiments were performed in 2 L bench top glass fermentors (Biostat B, Sartorius Stedim, Göttingen, Germany) equipped with two 6-blade Rushton impellers ...
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bioRxiv - Molecular Biology 2023Quote: ... The cells were resuspended in 2 mL 50 mM Tris buffer (pH 7.6) and lysed by using a dismembrator (Sartorius, Göttingen, Germany) at 1,600 rpm for 3 min ...
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bioRxiv - Microbiology 2023Quote: ... Cells were fixed with 2% paraformaldehyde and % GFP positive cells were determined using flow cytometry (Intellicyt iQue Screener PLUS, Sartorius AG). Infection was normalized to conditions without antibody ...
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bioRxiv - Plant Biology 2023Quote: ... 250 mM imidazole) and concentrated to 1-2 mL by ultrafiltration using a Vivaspin® Turbo Centrifugal Concentrator (Sartorius, United Kingdom). To remove imidazole ...
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bioRxiv - Microbiology 2023Quote: ... cells were fixed in 2% paraformaldehyde and GFP positive cells were quantified by flow cytometry (Intellicyt iQue Screener Plus, Sartorius AG). For experiments using single dilutions of serum or ExpiCHO-S cell supernatant ...
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bioRxiv - Cell Biology 2023Quote: ... as well as phase) were taken every 2 h over a period of 72 h using the IncuCyte® (Sartorius, Germany). General macroautophagy flux was calculated via the ratio of the total integrated fluorescence intensities of GFP/RFP ...
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bioRxiv - Plant Biology 2024Quote: ... Fractions were pooled and concentrated to a final concentration of 2 mg/ml using a centrifugal filter device (Sartorius, Göttingen, Germany) with a molecular weight cut-off of 50 kDa.
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bioRxiv - Genomics 2021Quote: ... Each diluted blood sample was then filtered using a 5 μm surfactant-free cellulose acetate syringe filter (Minisart NML® syringe filter, Sartorius reference number: 17594K) connected to a 10 mL syringe (Figure 1) ...
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bioRxiv - Biochemistry 2020Quote: ... glycerol was added and the protein concentrated to 20 – 30 mg/mL in a 10 kDa MWCO spin concentrator (Vivaspin, Sartorius).
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bioRxiv - Molecular Biology 2020Quote: ... The resulting CM was then concentrated to approximately 10 ml using the Vivaflow 50R tangential flow device (Sartorius, Göttingen, Germany) with a 100 kDa cut-off membrane ...
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bioRxiv - Microbiology 2020Quote: ... Protein samples were quantified by BCA assay (Pierce) and transferred to a 10 kDa MWCO spin filter (Vivaspin 500; Sartorius) before centrifugation at 12,000 x g to collect denatured and reduced proteins atop the filter membrane ...