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Citations for Sartorius :
101 - 150 of 246 citations for 6 PYRIDIN 2 YLMETHYL PYRIDAZIN 3 OL since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... beads were resuspended in FACS buffer and acquired on flow cytometer iQue®3 (Sartorius). Population gating and analysis of fluorescence intensity were performed with iQue Forecyt software ...
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bioRxiv - Biochemistry 2024Quote: ... and concentrated over a 3 kDa MWCO membrane using a Vivaspin 15 centrifugal device (Sartorius). The final Ws cytochrome c552 fraction was buffer exchanged to 5 mM Na-phosphate pH 7.0 using a desalting PD-10 column (Cytiva) ...
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bioRxiv - Molecular Biology 2022Quote: ... were mixed together as well as eluates from samples (Pho92-HTP x 2 reps + Pho92-HTP ime4Δ/Δ x 2 reps) before concentration with Vivacon® 500 filtration cartridges (10 kDa MWCO; Sartorius, Cat# VN01H02) to a final volume of 120 μL ...
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bioRxiv - Biophysics 2021Quote: ... using Vivaspin 2 columns (10 kDa molecular weight cutoff, Sartorius). The resulting ...
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bioRxiv - Microbiology 2021Quote: ... Fractions of IMAC eluate containing Pdr5 were pooled and concentrated using a Vivaspin 6 centrifugal concentrator unit (Sartorius AG, Göttingen, Germany) with a molecular weight cut-off (MWCO ...
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bioRxiv - Microbiology 2023Quote: ... A sample of 6 liters of air per min was collected during the <10 min exposure on the 47 mm gelatin filter (Sartorius, Germany). Post exposure ...
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bioRxiv - Plant Biology 2021Quote: ... and loaded onto an ultrafiltration column (Vivaspin 3 or 10 kDa MWCO, PES, Sartorius, Palaiseau, France) for concentration and buffer exchange at 4 °C ...
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bioRxiv - Molecular Biology 2019Quote: ... Mixed media were subsequently concentrated through spin columns with a cutoff of 3 kDa (Vivaspin, Sartorius). Mixed proteins were separated on a 4-20% Mini-PROTEAN TGX precast gel (Bio-Rad ...
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bioRxiv - Molecular Biology 2021Quote: ... The protein was concentrated to 3 mg/ml using a 50 kDa cutoff concentrator (Vivaspin, Sartorius). The purified protein was flash frozen in liquid nitrogen and stored at − 80 °C.
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bioRxiv - Developmental Biology 2022Quote: ... EpiLC differentiation medium was added and supplemented with 5µM of Caspase 3/7 Green dye (Sartorius) to detect for apoptosis in live cells ...
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bioRxiv - Microbiology 2023Quote: ... The filtered medium was then concentrated by ultrafiltration using Vivaspin 20 (3 kDa cut-off, Sartorius). Protein concentration was determined using a BCA protein assay kit (Beyotime ...
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bioRxiv - Immunology 2023Quote: ... they were concentrated using Vivaspin centrifugal concentrators with a cut-off of 3 kDa (Sartorius, VS2091) and stored at -20°C ...
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bioRxiv - Microbiology 2024Quote: ... The supernatants containing the fibrils were recovered and concentrated on vivaspin® 3 KDa (Sartorius, VS04T91) at 3,000 g ...
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bioRxiv - Cancer Biology 2021Quote: ... Time-lapse phase-contrast and fluorescent channel images are obtained at 6 hour intervals for up to 24 spheroids for each initial condition using an Incucyte S3 (Sartorius, Goettingen, Germany).
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bioRxiv - Biochemistry 2020Quote: ... The peak fractions containing ANT(9) were pooled and concentrated to 6 mg/ml using a Vivaspin 10 kDa cut-off concentrator (Sartorius AG, Germany).
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bioRxiv - Biophysics 2023Quote: ... proteins were exchanged to labelling buffer (20 mM HEPES, 50 mM NaCl, pH 7.5) using VivaSpin 6 concentrator (Sartorius AG, Gottingen, Germany).
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bioRxiv - Synthetic Biology 2020Quote: ... filtered through a 10 kDa MWCO spin filter (Vivaspin 2; Sartorius), and quantified by Nanodrop OneC ...
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bioRxiv - Molecular Biology 2021Quote: ... depth filtered supernatants were sterile filtered through a Sartopore 2 (Sartorius) filter unit and stored until further processing at 2-8 °C.
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bioRxiv - Animal Behavior and Cognition 2019Quote: ... but visually identifiable amount (Supplementary Figure 3) by weighing powder on a microbalance (Sartorius BP 210 D). One hour before the release ...
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bioRxiv - Microbiology 2022Quote: ... Homogenates were centrifuged for 3 min at 17,000g and filtered using a 0.8 μm PES filter (Sartorius). Filtrates were treated with micrococcal nuclease (New England Biolabs ...
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bioRxiv - Biochemistry 2023Quote: ... Samples were then diafiltered at 4°C using 3 kDa cutoff VivaSpin®500 centrifuge filters (Sartorius) for 3 cycles of 20 min at 14,000 rpm ...
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bioRxiv - Microbiology 2023Quote: ... Proteolytic DEVD cleaving activity was determined using the Incucyte Caspase-3/7 Red Dye for Apoptosis (Sartorius) as described in the manufacturer’s protocol ...
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bioRxiv - Synthetic Biology 2021Quote: ... The required amount of antibody was diluted in PBS (5ml) and applied to a Vivaspin 6 concentrator (Sartorius; VS0601; MWCO: 10kDa; 5ml volume). After centrifugation (4000 x g ...
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bioRxiv - Biochemistry 2020Quote: ... we concentrated the proteins from the supernate by membrane centrifugation using 5 K molecular weight cutoff Vivaspin units of 6 mL (Sartorius Stedim, Goettingen, Germany). The retentate (~300 μL ...
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bioRxiv - Microbiology 2021Quote: ... cell culture supernatant was centrifuged for 3 min at 17,000 × g and filtered with a 0.8-μm PES filter (Sartorius). The filtrate was treated with benzonase (Novagen ...
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bioRxiv - Microbiology 2020Quote: ... The homogenates were centrifuged for 3 min at 17,000 g and filtered with 0,8 μm PES filters (Sartorius). Filtrates were treated with benzonase (Novagen ...
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bioRxiv - Physiology 2022Quote: ... 2 mM DTT) using a Vivaspin-20 concentrator (catalog no. VS2021, Sartorius). Five buffer exchanges with the storage buffer were performed and purified MBP was then aliquoted and stored at −80°C.
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bioRxiv - Microbiology 2022Quote: ... and further concentrated to ∼100 µL using vivaspin 2 ultrafiltration units (Sartorius). All units had polyether sulphone filters with a molecular weight cut-off of 10 kDa ...
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bioRxiv - Synthetic Biology 2021Quote: ... The required amount of tomato lectin was diluted in PBS (5ml) and applied to a Vivaspin 6 concentrator (Sartorius; VS0601; MWCO: 10kDa; 5ml volume). After centrifugation (4000 x g ...
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bioRxiv - Microbiology 2019Quote: ... and concentrated to a final volume of 500 µL in a 3 kDa MWCO Vivaspin centrifugal filtration unit (Sartorius). The protein was detected by SDS-PAGE (Supplementary Fig ...
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bioRxiv - Neuroscience 2020Quote: ... the iodixanol was removed from the AAV vector containing fraction by 3 rounds of diafiltration using Vivaspin 20 ultrafiltration devices (100’000 MWCO, PES membrane, Sartorius) and 1x phosphate buffered saline (PBS ...
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bioRxiv - Microbiology 2023Quote: ... Cytiva) in 50 mM sodium acetate (pH 5.2) and concentrated using Vivaspin polyethersulfone membrane (3 kDa cut-off, Sartorius). Protein concentrations were determined using the Bradford method (Bio-Rad ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... in the presence of a green mortality probe (1/3000, Incucyte® Caspase-3/7 Green Dye - Sartorius, Germany). Green fluorescence was immediately followed every 90 or 120 minutes with an Incucyte® S3 Live-Cell Analysis system (Sartorius ...
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bioRxiv - Microbiology 2023Quote: ... the samples were centrifuged at 17,000 g for 3 min and 150 µl supernatant of each sample was subsequently filtered through a 0.8 µm filter (Sartorius). This filtrate was treated with a mix of Benzonase (50 U ...
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bioRxiv - Bioengineering 2019Quote: ... and disrupted with a micro-dismembrator (Sartorius, 2×30 s at 3,000 rpm). Subsequently ...
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bioRxiv - Molecular Biology 2021Quote: ... injection grade and passed through a 0.45 + 0.2 µm Sartopore 2 300 (Sartorius) filter for sterile filtration ...
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bioRxiv - Molecular Biology 2021Quote: ... Buffer exchanged supernatants were filtered using 0.8 + 0.45 µm Sartopore 2 filters (Sartorius) particulate contaminant removal and further sterile filtered using 0.2 µm Sartopore 2 filters (Sartorius) ...
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bioRxiv - Neuroscience 2022Quote: ... then plates were imaged every 2 hours using an Incucyte Zoom system (Sartorius) to quantify the number of fluorescent cells per field of view.
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bioRxiv - Biochemistry 2019Quote: The flow through from the above column was concentrated to 3 mL using a 10 kDa centrifugal concentrator (Sartorius Stedim) and loaded onto a pre-equilibrated (with buffer D ...
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bioRxiv - Evolutionary Biology 2020Quote: ... We further weighted 3-week old plants with a precision balance (Sartorius AC 210 P with accuracy of 0.1 mg) to quantify variation in plant biomass ...
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bioRxiv - Biochemistry 2020Quote: ... were exchanged into phosphate buffer (pH 7.4) using disposable Vivaspin® 500 centrifugal concentrators with an MWCO of 3 kDa (Sartorius) to remove Cu2+ or H202 ...
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bioRxiv - Biophysics 2021Quote: ... The fractions corresponding to hIP3R-3 were combined and concentrated to 4 mg/mL using a 100 kDa centrifugal filter (Sartorius). The concentrated sample was then centrifuged at 70,000 rpm using a S100AT rotor (Thermofisher) ...
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bioRxiv - Biophysics 2021Quote: ... and 0.01 mg/mL 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) were added before concentration in Vivaspin® 100,000 kDa molecular weight cut-off (MWCO) centrifugal concentrators (Sartorius) to a volume of ∼500 μL ...
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bioRxiv - Biochemistry 2020Quote: ... Unreacted propargylamine was removed by size exclusion chromatography and Ub/LC3B-PA was concentrated using VIVASPIN 20 Columns (3 kDa cutoff, Sartorius), flash frozen and stored at −80 °C.
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bioRxiv - Plant Biology 2019Quote: ... The protein without His-Tag was concentrated using a Vivaspin 20 Ultrafiltration Unit (3 kDa molecular weight cutoff, Sartorius, www.sartorius.de). An appropriate volume was applied to a 1 ml loop of a gel filtration column (Superdex75 10/300 GL ...
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bioRxiv - Biochemistry 2021Quote: ... Proteins were additionally purified through a Superdex 200 26/60 size exclusion column (Pharmacia Biotech) and concentrated using a Vivaspin Turbo 3 kDa concentrator (Sartorius). Protein concentrations were measured using a UV/Vis spectrophotometer (GE Healthcare ...
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bioRxiv - Immunology 2021Quote: ... S2K146 and respective UCA Ab were prepared at 3 μg/ml and immobilized on pre-hydrated protein-A biosensors (Sartorius) for 75 sec ...
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bioRxiv - Biochemistry 2023Quote: ... Unreacted propargylamine was removed by size exclusion chromatography and the probes were concentrated using VIVASPIN 20 Columns (3 kDa cutoff, Sartorius), flash frozen and stored at −80 °C ...
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bioRxiv - Synthetic Biology 2022Quote: ... passivated (with 20 μL of 50 mM ABC in 1% sodium deoxycholate or SDC) Vivacon 500 3 kDa molecular weight cutoff (MWCO) filtration units from Sartorius Stedim Biotech ...
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bioRxiv - Biophysics 2023Quote: ... or PPIase) were buffer-exchanged into an NMR buffer using either 5 or 3 kDa MWCO centrifugal concentrators (Vivaspin, Sartorius). The NMR buffer contained 10 mM d4-imidazole at pH 6.6 ...